{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:34:19Z","timestamp":1773300859768,"version":"3.50.1"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"KCVI funding"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Prostaglandins & Other Lipid Mediators"],"published-print":{"date-parts":[[2017,9]]},"DOI":"10.1016\/j.prostaglandins.2016.09.002","type":"journal-article","created":{"date-parts":[[2016,9,18]],"date-time":"2016-09-18T16:16:11Z","timestamp":1474215371000},"page":"31-40","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["Functional screening for G protein-coupled receptor targets of 14,15-epoxyeicosatrienoic acid"],"prefix":"10.1016","volume":"132","author":[{"given":"Xuehong","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zu-yuan","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuchun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan W.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangshu","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjiv","family":"Kaul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P.","family":"Barnes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabil J.","family":"Alkayed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0005","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1161\/01.RES.83.9.932","article-title":"Epoxyeicosatrienoic acids and dihydroxyeicosatrienoic acids are potent vasodilators in the canine coronary microcirculation","volume":"83","author":"Oltman","year":"1998","journal-title":"Circ. Res."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.prostaglandins.2014.05.001","article-title":"Vasodilatory effect of 14,15-epoxyeicosatrienoic acid on mesenteric arteries in hypertensive and aged rats","volume":"112","author":"Yang","year":"2014","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0015","doi-asserted-by":"crossref","first-page":"3840","DOI":"10.1021\/bi102070w","article-title":"20-Iodo-14,15-epoxyeicosa-8(Z)-enoyl-3-azidophenylsulfonamide: photoaffinity labeling of a 14,15-epoxyeicosatrienoic acid receptor","volume":"50","author":"Chen","year":"2011","journal-title":"Biochemistry"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0020","doi-asserted-by":"crossref","first-page":"H337","DOI":"10.1152\/ajpheart.00831.2001","article-title":"Comparison of vasodilatory properties of 14,15-EET analogs: structural requirements for dilation","volume":"284","author":"Falck","year":"2003","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0025","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.phrs.2003.09.014","article-title":"14,15-EET analogs: characterization of structural requirements for agonist and antagonist activity in bovine coronary arteries","volume":"49","author":"Gauthier","year":"2004","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0030","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.prostaglandins.2010.06.004","article-title":"14,15-Epoxyeicosatrienoic acid induces vasorelaxation through the prostaglandin EP(2) receptors in rat mesenteric artery","volume":"93","author":"Yang","year":"2010","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0035","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.biocel.2014.02.020","article-title":"Inhibitory effect of 14,15-EET on endothelial senescence through activation of mTOR complex 2\/Akt signaling pathways","volume":"50","author":"Yang","year":"2014","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0040","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1124\/jpet.107.129577","article-title":"Characterization of epoxyeicosatrienoic acid binding site in U937 membranes using a novel radiolabeled agonist, 20-125i-14, 15-epoxyeicosa-8(Z)-enoic acid","volume":"324","author":"Yang","year":"2008","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0045","doi-asserted-by":"crossref","first-page":"H2430","DOI":"10.1152\/ajpheart.2001.280.6.H2430","article-title":"EET homologs potently dilate coronary microvessels and activate BK(Ca) channels","volume":"280","author":"Zhang","year":"2001","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0050","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.prostaglandins.2009.06.004","article-title":"Epoxyeicosanoid signaling in CNS function and disease","volume":"91","author":"Iliff","year":"2010","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0055","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1124\/jpet.109.157818","article-title":"20-125Iodo-14,15-epoxyeicosa-5(Z)-enoic acid: a high-affinity radioligand used to characterize the epoxyeicosatrienoic acid antagonist binding site","volume":"331","author":"Chen","year":"2009","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0060","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0090-6980(00)00079-4","article-title":"Mechanism and signal transduction of 14 (R), 15 (S)-epoxyeicosatrienoic acid (14,15-EET) binding in guinea pig monocytes","volume":"62","author":"Wong","year":"2000","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0065","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0929-7855(97)00005-9","article-title":"Post-receptor signal transduction and regulation of 14(R),15(S)-epoxyeicosatrienoic acid (14,15-EET) binding in U-937 cells","volume":"16","author":"Wong","year":"1997","journal-title":"J. Lipid Mediat. Cell Signal."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0070","first-page":"199","article-title":"14(R),15(S)-epoxyeicosatrienoic acid (14(R),15(S)-EET) receptor in guinea pig mononuclear cell membranes","volume":"6","author":"Wong","year":"1993","journal-title":"J. Lipid Mediat."},{"issue":"Suppl. 10","key":"10.1016\/j.prostaglandins.2016.09.002_bib0075","first-page":"179","article-title":"CFTR activation: stimulatory and inhibitory phosphorylation sites in the R domain","author":"Wilkinson","year":"1994","journal-title":"Pediatr. Pulmonol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0080","doi-asserted-by":"crossref","first-page":"S77","DOI":"10.1152\/physrev.1999.79.1.S77","article-title":"Control of CFTR channel gating by phosphorylation and nucleotide hydrolysis","volume":"79","author":"Gadsby","year":"1999","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0085","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1006\/abio.1995.1248","article-title":"Detection of adenylate cyclase-coupled receptors in Xenopus oocytes by coexpression with cystic fibrosis transmembrane conductance regulator","volume":"227","author":"Grygorczyk","year":"1995","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0090","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1124\/jpet.114.213116","article-title":"Ractopamine, a livestock feed additive, is a full agonist at trace amine-associated receptor 1","volume":"350","author":"Liu","year":"2014","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0095","first-page":"233","article-title":"Receptors that couple to 2 classes of G proteins increase cAMP and activate CFTR expressed in Xenopus oocytes","volume":"1","author":"Uezono","year":"1993","journal-title":"Recept. Channels"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0100","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.steroids.2006.11.006","article-title":"G beta gamma signaling reduces intracellular cAMP to promote meiotic progression in mouse oocytes","volume":"72","author":"Gill","year":"2007","journal-title":"Steroids"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0105","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/00397911.2014.956369","article-title":"Synthesis of 14,15-EET from arachidonic acid using urea-hydrogen peroxide as the oxidant","volume":"45","author":"Xie","year":"2015","journal-title":"Synth. Commun."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0110","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1388-1981(99)00164-X","article-title":"The utilization of recombinant prostanoid receptors to determine the affinities and selectivities of prostaglandins and related analogs","volume":"1483","author":"Abramovitz","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0115","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1159\/000057320","article-title":"Diversity of G protein-coupled receptor signaling pathways to ERK\/MAP kinase","volume":"11","author":"Belcheva","year":"2002","journal-title":"Neurosignals"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0120","article-title":"Assay Guidance Manual","author":"Garbison","year":"2004"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0125","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1038\/358761a0","article-title":"Processing of mutant cystic fibrosis transmembrane conductance regulator is temperature-sensitive [see comments]","volume":"358","author":"Denning","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0130","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1124\/mol.51.2.343","article-title":"The long cytoplasmic carboxyl terminus of the prostaglandin E2 receptor EP4 subtype is essential for agonist-induced desensitization","volume":"51","author":"Bastepe","year":"1997","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0135","doi-asserted-by":"crossref","first-page":"11873","DOI":"10.1016\/S0021-9258(17)32654-6","article-title":"Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype","volume":"269","author":"Bastien","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0140","doi-asserted-by":"crossref","first-page":"11613","DOI":"10.1074\/jbc.R600038200","article-title":"Prostaglandin E. receptors","volume":"282","author":"Sugimoto","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0145","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/sj.bjp.0701708","article-title":"Characterization of the recombinant human prostanoid DP receptor and identification of L-644, 698, a novel selective DP agonist","volume":"123","author":"Wright","year":"1998","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0150","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/S0026-895X(25)10545-2","article-title":"Molecular cloning and expression of multiple isoforms of human prostaglandin E receptor EP3 subtype generated by alternative messenger RNA splicing: multiple second messenger systems and tissue-specific distributions","volume":"48","author":"Kotani","year":"1995","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0155","doi-asserted-by":"crossref","first-page":"493","DOI":"10.3109\/10799893.2015.1015738","article-title":"ANP and CNP activate CFTR expressed in Xenopus laevis oocytes by direct activation of PKA","volume":"35","author":"Stahl","year":"2015","journal-title":"J. Recept. Signal Transduct. Res."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0160","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1161\/01.RES.0000018162.87285.F8","article-title":"14,15-Epoxyeicosa-5(Z)-enoic acid: a selective epoxyeicosatrienoic acid antagonist that inhibits endothelium-dependent hyperpolarization and relaxation in coronary arteries","volume":"90","author":"Gauthier","year":"2002","journal-title":"Circ. Res."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0165","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.ejphar.2004.09.062","article-title":"Not so EEZE: the \u2018EDHF\u2019 antagonist 14, 15 epoxyeicosa-5(Z)-enoic acid has vasodilator properties in mesenteric arteries","volume":"506","author":"Harrington","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0170","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1172\/JCI0216112","article-title":"Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion","volume":"110","author":"Ma","year":"2002","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0175","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1248\/yakushi.123.837","article-title":"Cooperation of two subtypes of PGE2 receptor, Gi coupled EP3 and Gs coupled EP2 or EP4 subtype","volume":"123","author":"Hatae","year":"2003","journal-title":"Yakugaku Zasshi: J. Pharm. Soc. Japan"},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0180","doi-asserted-by":"crossref","first-page":"18910","DOI":"10.1074\/jbc.270.32.18910","article-title":"Molecular cloning and characterization of the human prostanoid DP receptor","volume":"270","author":"Boie","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0185","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1042\/bj3510095","article-title":"Specificity and mechanism of action of some commonly used protein kinase inhibitors","volume":"351","author":"Davies","year":"2000","journal-title":"Biochem. J."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0190","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/j.1527-3466.2006.00261.x","article-title":"The many faces of H89: a review","volume":"24","author":"Lochner","year":"2006","journal-title":"Cardiovasc. Drug Rev."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0195","doi-asserted-by":"crossref","first-page":"re4","DOI":"10.1126\/scisignal.122re4","article-title":"Pharmacological PKA inhibition: all may not be what it seems","volume":"1","author":"Murray","year":"2008","journal-title":"Sci. Signal."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0200","doi-asserted-by":"crossref","first-page":"2632","DOI":"10.1016\/S0021-9258(17)41991-0","article-title":"Cloning and expression of a cDNA for the human prostanoid FP receptor","volume":"269","author":"Abramovitz","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0205","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1016\/0006-291X(92)91877-S","article-title":"Arachidonic acid epoxygenase: structural characterization and quantification of epoxyeicosatrienoates in plasma","volume":"182","author":"Karara","year":"1992","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0210","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jchromb.2008.10.035","article-title":"Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC\/MS in the NICI mode","volume":"876","author":"Goulitquer","year":"2008","journal-title":"J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0215","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.bbrc.2007.05.145","article-title":"Indomethacin decreases EP2 prostanoid receptor expression in colon cancer cells","volume":"359","author":"Fujino","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.prostaglandins.2016.09.002_bib0220","doi-asserted-by":"crossref","first-page":"2831","DOI":"10.4049\/jimmunol.167.5.2831","article-title":"Roles of cyclooxygenase (COX)-1 and COX-2 in prostanoid production by human endothelial cells: selective up-regulation of prostacyclin synthesis by COX-2","volume":"167","author":"Caughey","year":"2001","journal-title":"J. Immunol. (Baltim., Md.: 1950)"}],"container-title":["Prostaglandins & Other Lipid Mediators"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1098882316301095?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1098882316301095?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T19:46:56Z","timestamp":1749584816000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1098882316301095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":44,"alternative-id":["S1098882316301095"],"URL":"https:\/\/doi.org\/10.1016\/j.prostaglandins.2016.09.002","relation":{},"ISSN":["1098-8823"],"issn-type":[{"value":"1098-8823","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functional screening for G protein-coupled receptor targets of 14,15-epoxyeicosatrienoic acid","name":"articletitle","label":"Article Title"},{"value":"Prostaglandins & Other Lipid Mediators","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.prostaglandins.2016.09.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}