{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:46:09Z","timestamp":1791107169136,"version":"4.1.0"},"reference-count":52,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":380,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protist"],"published-print":{"date-parts":[[2012,7]]},"DOI":"10.1016\/j.protis.2011.10.010","type":"journal-article","created":{"date-parts":[[2011,12,18]],"date-time":"2011-12-18T21:31:56Z","timestamp":1324243916000},"page":"602-615","source":"Crossref","is-referenced-by-count":29,"title":["A Trypanosoma brucei Protein Required for Maintenance of the Flagellum Attachment Zone and Flagellar Pocket ER Domains"],"prefix":"10.1016","volume":"163","author":[{"given":"Sylvain","family":"Lacomble","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Vaughan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Deghelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Fernandes","family":"Moreira-Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Gull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.protis.2011.10.010_bib0005","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1242\/jcs.035626","article-title":"Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei","volume":"121","author":"Absalon","year":"2008","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0010","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1101\/gr.115089.110","article-title":"High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome","volume":"21","author":"Alsford","year":"2011","journal-title":"Genome Res"},{"key":"10.1016\/j.protis.2011.10.010_bib0015","doi-asserted-by":"crossref","first-page":"5934","DOI":"10.1074\/jbc.M409566200","article-title":"Differential regulation of endoplasmic reticulum structure through VAP-Nir protein interaction","volume":"280","author":"Amarilio","year":"2005","journal-title":"J Biol Chem"},{"key":"10.1016\/j.protis.2011.10.010_bib0020","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(96)02598-4","article-title":"A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei","volume":"77","author":"Bastin","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.protis.2011.10.010_bib0025","doi-asserted-by":"crossref","first-page":"e342","DOI":"10.1371\/journal.pbio.0020342","article-title":"Gene recruitment of the activated INO1 locus to the nuclear membrane","volume":"2","author":"Brickner","year":"2004","journal-title":"PLoS Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0030","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/nature04541","article-title":"Flagellar motility is required for the viability of the bloodstream trypanosome","volume":"440","author":"Broadhead","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.protis.2011.10.010_bib0035","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1242\/jcs.03203","article-title":"Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation","volume":"119","author":"Davidge","year":"2006","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0040","doi-asserted-by":"crossref","first-page":"e8468","DOI":"10.1371\/journal.pone.0008468","article-title":"Chaperone requirements for biosynthesis of the trypanosome variant surface glycoprotein","volume":"5","author":"Field","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.protis.2011.10.010_bib0045","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1128\/EC.00110-07","article-title":"RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection","volume":"6","author":"Griffiths","year":"2007","journal-title":"Eukaryot Cell"},{"key":"10.1016\/j.protis.2011.10.010_bib0050","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1038\/emboj.2009.203","article-title":"Distinct donor and acceptor specificities of Trypanosoma brucei oligosaccharyltransferases","volume":"28","author":"Izquierdo","year":"2009","journal-title":"EMBO J"},{"key":"10.1016\/j.protis.2011.10.010_bib0055","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1093\/jb\/mvg068","article-title":"Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism","volume":"133","author":"Kagiwada","year":"2003","journal-title":"J Biochem"},{"key":"10.1016\/j.protis.2011.10.010_bib0060","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1128\/JB.180.7.1700-1708.1998","article-title":"The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism","volume":"180","author":"Kagiwada","year":"1998","journal-title":"J Bacteriol"},{"key":"10.1016\/j.protis.2011.10.010_bib0065","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A combined transmembrane topology and signal peptide prediction method","volume":"338","author":"Kall","year":"2004","journal-title":"J Mol Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0070","doi-asserted-by":"crossref","first-page":"30223","DOI":"10.1074\/jbc.M605049200","article-title":"Characterization of amyotrophic lateral sclerosis-linked P56S mutation of vesicle-associated membrane protein-associated protein B (VAPB\/ALS8)","volume":"281","author":"Kanekura","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.protis.2011.10.010_bib0075","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-1-59745-251-9_3","article-title":"Multiple alignment of DNA sequences with MAFFT","volume":"537","author":"Katoh","year":"2009","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0080","doi-asserted-by":"crossref","first-page":"30279","DOI":"10.1074\/jbc.M605032200","article-title":"Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT","volume":"281","author":"Kawano","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.protis.2011.10.010_bib0085","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.molbiopara.2007.03.012","article-title":"Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci","volume":"154","author":"Kelly","year":"2007","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.protis.2011.10.010_bib0090","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.1093\/emboj\/cdg518","article-title":"Novel roles for the flagellum in cell morphogenesis and cytokinesis of trypanosomes","volume":"22","author":"Kohl","year":"2003","journal-title":"EMBO J"},{"key":"10.1016\/j.protis.2011.10.010_bib0095","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1550-7408.1999.tb04592.x","article-title":"Assembly of the paraflagellar rod and the flagellum attachment zone complex during the Trypanosoma brucei cell cycle","volume":"46","author":"Kohl","year":"1999","journal-title":"J Eukaryot Microbiol"},{"key":"10.1016\/j.protis.2011.10.010_bib0100","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1186\/1471-2164-9-298","article-title":"The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages","volume":"9","author":"Koumandou","year":"2008","journal-title":"BMC Genomics"},{"key":"10.1016\/j.protis.2011.10.010_bib0105","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"Kremer","year":"1996","journal-title":"J Struct Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0110","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1242\/jcs.045740","article-title":"Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography","volume":"122","author":"Lacomble","year":"2009","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0115","doi-asserted-by":"crossref","first-page":"e7685","DOI":"10.1371\/journal.pone.0007685","article-title":"A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod","volume":"4","author":"Lacomble","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.protis.2011.10.010_bib0120","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1242\/jcs.074161","article-title":"Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei","volume":"123","author":"Lacomble","year":"2010","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0125","doi-asserted-by":"crossref","first-page":"17580","DOI":"10.1074\/jbc.M200873200","article-title":"Trypanosoma brucei FLA1 is required for flagellum attachment and cytokinesis","volume":"277","author":"LaCount","year":"2002","journal-title":"J Biol Chem"},{"key":"10.1016\/j.protis.2011.10.010_bib0130","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.tcb.2008.03.006","article-title":"The VAP protein family: from cellular functions to motor neuron disease","volume":"18","author":"Lev","year":"2008","journal-title":"Trends Cell Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0135","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1083\/jcb.200708205","article-title":"Inheritance of cortical ER in yeast is required for normal septin organization","volume":"179","author":"Loewen","year":"2007","journal-title":"J Cell Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0140","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1093\/oxfordjournals.jbchem.a124889","article-title":"Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae","volume":"118","author":"Nikawa","year":"1995","journal-title":"J Biochem"},{"key":"10.1016\/j.protis.2011.10.010_bib0145","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s00439-005-0031-y","article-title":"A common founder for amyotrophic lateral sclerosis type 8 (ALS8) in the Brazilian population","volume":"118","author":"Nishimura","year":"2005","journal-title":"Hum Genet"},{"key":"10.1016\/j.protis.2011.10.010_bib0150","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1086\/425287","article-title":"A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis","volume":"75","author":"Nishimura","year":"2004","journal-title":"Am J Hum Genet"},{"key":"10.1016\/j.protis.2011.10.010_bib0155","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1006\/bbrc.1998.9876","article-title":"Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins","volume":"254","author":"Nishimura","year":"1999","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.protis.2011.10.010_bib0160","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0166-6851(96)02741-7","article-title":"Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein","volume":"82","author":"Nozaki","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.protis.2011.10.010_bib0165","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1091\/mbc.E02-08-0525","article-title":"A high-order trans-membrane structural linkage is responsible for mitochondrial genome positioning and segregation by flagellar basal bodies in trypanosomes","volume":"14","author":"Ogbadoyi","year":"2003","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.protis.2011.10.010_bib0170","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0896-6273(02)00769-9","article-title":"Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner","volume":"35","author":"Pennetta","year":"2002","journal-title":"Neuron"},{"key":"10.1016\/j.protis.2011.10.010_bib0175","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijpara.2009.10.005","article-title":"The paraflagellar rod of kinetoplastid parasites: from structure to components and function","volume":"40","author":"Portman","year":"2010","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.protis.2011.10.010_bib0180","doi-asserted-by":"crossref","first-page":"3052","DOI":"10.1242\/jcs.028696","article-title":"FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation","volume":"121","author":"Prosser","year":"2008","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0185","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1128\/EC.00298-10","article-title":"Structure-function analysis of dynein light chain 1 identifies viable motility mutants in bloodstream-form Trypanosoma brucei","volume":"10","author":"Ralston","year":"2011","journal-title":"Eukaryot Cell"},{"key":"10.1016\/j.protis.2011.10.010_bib0190","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0955-0674(95)80039-5","article-title":"Nematode sperm locomotion","volume":"7","author":"Roberts","year":"1995","journal-title":"Curr Opin Cell Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0195","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/352731a0","article-title":"Basal body movements as a mechanism for mitochondrial genome segregation in the trypanosome cell cycle","volume":"352","author":"Robinson","year":"1991","journal-title":"Nature"},{"key":"10.1016\/j.protis.2011.10.010_bib0200","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1083\/jcb.128.6.1163","article-title":"Microtubule polarity and dynamics in the control of organelle positioning, segregation, and cytokinesis in the trypanosome cell cycle","volume":"128","author":"Robinson","year":"1995","journal-title":"J Cell Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0205","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jsb.2005.11.008","article-title":"The flagellar attachment zone of Trypanosoma cruzi epimastigote forms","volume":"154","author":"Rocha","year":"2006","journal-title":"J Struct Biol"},{"key":"10.1016\/j.protis.2011.10.010_bib0210","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1091\/mbc.E09-07-0542","article-title":"Streamlined architecture and glycosylphosphatidylinositol-dependent trafficking in the early secretory pathway of African trypanosomes","volume":"20","author":"Sevova","year":"2009","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.protis.2011.10.010_bib0215","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1098\/rstb.1989.0037","article-title":"The cell division cycle of Trypanosoma brucei brucei: timing of event markers and cytoskeletal modulations","volume":"323","author":"Sherwin","year":"1989","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0220","doi-asserted-by":"crossref","first-page":"23636","DOI":"10.1074\/jbc.M803600200","article-title":"Phosphatidylethanolamine in Trypanosoma brucei is organized in two separate pools and is synthesized exclusively by the Kennedy pathway","volume":"283","author":"Signorell","year":"2008","journal-title":"J Biol Chem"},{"key":"10.1016\/j.protis.2011.10.010_bib0225","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1073\/pnas.97.3.1101","article-title":"Mouse VAP33 is associated with the endoplasmic reticulum and microtubules","volume":"97","author":"Skehel","year":"2000","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.protis.2011.10.010_bib0230","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1126\/science.7667638","article-title":"A VAMP-binding protein from Aplysia required for neurotransmitter release","volume":"269","author":"Skehel","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.protis.2011.10.010_bib0235","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1111\/j.1550-7408.1969.tb02302.x","article-title":"A new organelle of bloodstream salivarian trypanosomes","volume":"16","author":"Taylor","year":"1969","journal-title":"J Protozool"},{"key":"10.1016\/j.protis.2011.10.010_bib0240","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/jcs.74.1.1","article-title":"Differentiation in Trypanosoma brucei: host-parasite cell junctions and their persistence during acquisition of the variable antigen coat","volume":"74","author":"Tetley","year":"1985","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0245","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.mib.2010.05.006","article-title":"Assembly of the flagellum and its role in cell morphogenesis in Trypanosoma brucei","volume":"13","author":"Vaughan","year":"2010","journal-title":"Curr Opin Microbiol"},{"key":"10.1016\/j.protis.2011.10.010_bib0250","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.protis.2007.08.005","article-title":"A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei","volume":"159","author":"Vaughan","year":"2008","journal-title":"Protist"},{"key":"10.1016\/j.protis.2011.10.010_bib0255","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1242\/jcs.5.1.163","article-title":"On the surface coat and flagellar adhesion in trypanosomes","volume":"5","author":"Vickerman","year":"1969","journal-title":"J Cell Sci"},{"key":"10.1016\/j.protis.2011.10.010_bib0260","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1242\/jcs.95.1.49","article-title":"Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei","volume":"95","author":"Woodward","year":"1990","journal-title":"J Cell Sci"}],"container-title":["Protist"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1434461011001180?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1434461011001180?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T23:57:40Z","timestamp":1640044660000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1434461011001180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["S1434461011001180"],"URL":"https:\/\/doi.org\/10.1016\/j.protis.2011.10.010","relation":{},"ISSN":["1434-4610"],"issn-type":[{"value":"1434-4610","type":"print"}],"subject":[],"published":{"date-parts":[[2012,7]]}}}