{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:25:03Z","timestamp":1785684303852,"version":"3.56.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Feinberg School of Medicine, Northwestern University\u2019s Department of Pathology"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pathology - Research and Practice"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.prp.2020.153105","type":"journal-article","created":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T21:36:00Z","timestamp":1594503360000},"page":"153105","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["The diagnostic utility of PRAME and p16 in distinguishing nodal nevi from nodal metastatic melanoma"],"prefix":"10.1016","volume":"216","author":[{"given":"Sharlene Helene C.","family":"See","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian S.","family":"Finkelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjana V.","family":"Yeldandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.prp.2020.153105_bib0005","series-title":"Epidemiology, and End Results Program (SEER). Cancer stat Facts: Melanoma of the Skin","year":"2019"},{"key":"10.1016\/j.prp.2020.153105_bib0010","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/modpathol.2012.132","article-title":"Diagnostic utility of neural stem and progenitor cell markers nestin and SOX2 in distinguishing nodal melanocytic nevi from metastatic melanomas","volume":"26","author":"Chen","year":"2013","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0015","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s00428-003-0897-9","article-title":"Immunostaining for the tumour suppressor gene p16 product is a useful marker to differentiate melanoma metastasis from lymph-node nevus","volume":"443","author":"Mihic-Probst","year":"2003","journal-title":"Virch. Arch."},{"key":"10.1016\/j.prp.2020.153105_bib0020","doi-asserted-by":"crossref","first-page":"83","DOI":"10.5858\/arpa.2014-0075-RA","article-title":"Immunohistochemistry in dermatopathology","volume":"139","author":"Ferringer","year":"2015","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.prp.2020.153105_bib0025","doi-asserted-by":"crossref","first-page":"853","DOI":"10.5858\/2009-0717-RAR.1","article-title":"Immunohistochemistry of melanocytic proliferations","volume":"135","author":"Prieto","year":"2011","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.prp.2020.153105_bib0030","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1097\/00000478-200305000-00011","article-title":"Intraparenchymal nevus cell aggregates in lymph nodes: a possible diagnostic pitfall with malignant melanoma and carcinoma","volume":"27","author":"Biddle","year":"2003","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0035","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH729>3.0.CO;2-D","article-title":"Expression of gp100, MART-1, tyrosinase, and S100 in paraffin-embedded primary melanomas and locoregional, lymph node, and visceral metastases: implications for diagnosis and immunotherapy. A study conducted by the EORTC Melanoma Cooperative Group","volume":"193","author":"de Vries","year":"2001","journal-title":"J. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0040","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/j.1600-0560.2008.01154.x","article-title":"p16 expression differentiates between desmoplastic Spitz nevus and desmoplastic melanoma","volume":"36","author":"Hilliard","year":"2009","journal-title":"J. Cutan. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0045","first-page":"2713","article-title":"Loss of expression of the p16\/cyclin-dependent kinase inhibitor 2 tumor suppressor gene in melanocytic lesions correlates with invasive stage of tumor progression","volume":"55","author":"Reed","year":"1995","journal-title":"Cancer Res."},{"key":"10.1016\/j.prp.2020.153105_bib0050","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.prp.2015.01.003","article-title":"Lymph node melanocytic nevi: pathogenesis and differential diagnoses, with special reference to p16 reactivity","volume":"211","author":"Piana","year":"2015","journal-title":"Pathol. Res. Pract."},{"key":"10.1016\/j.prp.2020.153105_bib0055","doi-asserted-by":"crossref","first-page":"815","DOI":"10.5858\/arpa.2017-0435-RA","article-title":"Immunohistochemical expression of p16 in melanocytic lesions: an updated review and meta-analysis","volume":"142","author":"Koh","year":"2018","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.prp.2020.153105_bib0060","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S1074-7613(00)80426-4","article-title":"Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK receptor","volume":"6","author":"Ikeda","year":"1997","journal-title":"Immunity."},{"key":"10.1016\/j.prp.2020.153105_bib0065","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1158\/1078-0432.CCR-15-2071","article-title":"PRAME as an independent biomarker for metastasis in uveal melanoma","volume":"22","author":"Field","year":"2016","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.prp.2020.153105_bib0070","doi-asserted-by":"crossref","first-page":"59209","DOI":"10.18632\/oncotarget.10962","article-title":"Epigenetic reprogramming and aberrant expression of PRAME are associated with increased metastatic risk in Class 1 and Class 2 uveal melanomas","volume":"7","author":"Field","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.prp.2020.153105_bib0075","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1097\/PAS.0000000000001134","article-title":"PRAME expression in melanocytic tumors","volume":"42","author":"Lezcano","year":"2018","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0080","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1002\/path.2229","article-title":"Systematic search for the best gene expression markers for melanoma micrometastasis detection","volume":"213","author":"Soikkeli","year":"2007","journal-title":"J. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0085","doi-asserted-by":"crossref","first-page":"567","DOI":"10.2307\/2533958","article-title":"Nonparametric analysis of clustered ROC curve data","volume":"53","author":"Obuchowski","year":"1997","journal-title":"Biometrics"},{"key":"10.1016\/j.prp.2020.153105_bib0090","series-title":"R: a Language and Environment for Statistical Computing","author":"Core Team","year":"2017"},{"key":"10.1016\/j.prp.2020.153105_bib0095","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1097\/00000478-199607000-00006","article-title":"Nodal nevi and cutaneous melanomas","volume":"20","author":"Carson","year":"1996","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0100","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/S0344-0338(85)80204-1","article-title":"Nevus cells in axillary lymph nodes from radical mastectomy specimens","volume":"179","author":"Andreola","year":"1985","journal-title":"Pathol. Res. Pract."},{"key":"10.1016\/j.prp.2020.153105_bib0105","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1093\/ajcp\/102.1.102","article-title":"Benign melanocytic nevus cells in axillary lymph nodes. A prospective incidence and immunohistochemical study with literature review","volume":"102","author":"Bautista","year":"1994","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0110","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1097\/00000478-200210000-00012","article-title":"Expression of melanocyte differentiation antigens and Ki-67 in nodal nevi and comparison of Ki-67 expression with metastatic melanoma","volume":"26","author":"Lohmann","year":"2002","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0115","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/cup.13412","article-title":"Diagnostic utility of dual 5-hydroxymethylcytosine\/Melan-A immunohistochemistry in differentiating nodal nevus from metastatic melanoma: an effective first-line test for the workup of sentinel lymph node specimen","volume":"46","author":"Siref","year":"2019","journal-title":"J. Cutan. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0120","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/modpathol.2014.99","article-title":"5-hydroxymethylcytosine expression in metastatic melanoma versus nodal nevus in sentinel lymph node biopsies","volume":"28","author":"Lee","year":"2015","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0125","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1097\/DAD.0000000000000939","article-title":"Fatty acid synthase and acetyl-CoA carboxylase are expressed in nodal metastatic melanoma but not in benign intracapsular nodal nevi","volume":"40","author":"Saab","year":"2018","journal-title":"Am. J. Dermatopathol."},{"key":"10.1016\/j.prp.2020.153105_bib0130","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1097\/DAD.0b013e318272c104","article-title":"Reticulin and NM23 staining in interpretation of lymph node nevus rests","volume":"35","author":"Kanner","year":"2013","journal-title":"Am. J. Dermatopathol."},{"key":"10.1016\/j.prp.2020.153105_bib0135","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1097\/PAS.0000000000001393","article-title":"Immunohistochemistry for PRAME in the distinction of nodal nevi from metastatic melanoma","volume":"44","author":"Lezcano","year":"2020","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0140","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1097\/PAS.0b013e3182237985","article-title":"Benign epithelial inclusions in axillary lymph nodes: report of 18 cases and review of the literature","volume":"35","author":"Fellegara","year":"2011","journal-title":"Am. J. Surg. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0145","article-title":"Expression of estrogen receptors in Spitz and Reed nevi","author":"Panasiti","year":"2019","journal-title":"G. Ital. Dermatol. Venereol."},{"key":"10.1016\/j.prp.2020.153105_bib0150","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/j.1600-0625.2007.00667.x","article-title":"Estrogen receptor beta expression in nevi during pregnancy","volume":"17","author":"Nading","year":"2008","journal-title":"Exp. Dermatol."},{"key":"10.1016\/j.prp.2020.153105_bib0155","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3109\/00313027409077346","article-title":"Naevus cells in lymph nodes","volume":"6","author":"McCarthy","year":"1974","journal-title":"Pathology"},{"key":"10.1016\/j.prp.2020.153105_bib0160","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1093\/ajcp\/55.1.88","article-title":"Primary nevus of a lymph node","volume":"55","author":"Hart","year":"1971","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.prp.2020.153105_bib0165","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1097\/00000478-198702000-00008","article-title":"Malignant melanoma primary in the lymph node: the case of the missing link","volume":"11","author":"Shenoy","year":"1987","journal-title":"Am. J. Surg. Pathol."}],"container-title":["Pathology - Research and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0344033820319609?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0344033820319609?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T06:46:05Z","timestamp":1759733165000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0344033820319609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["S0344033820319609"],"URL":"https:\/\/doi.org\/10.1016\/j.prp.2020.153105","relation":{},"ISSN":["0344-0338"],"issn-type":[{"value":"0344-0338","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The diagnostic utility of PRAME and p16 in distinguishing nodal nevi from nodal metastatic melanoma","name":"articletitle","label":"Article Title"},{"value":"Pathology - Research and Practice","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.prp.2020.153105","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"153105"}}