{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:15:55Z","timestamp":1759796155394,"version":"build-2065373602"},"reference-count":24,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100012774","name":"Innovationsfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pathology - Research and Practice"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.prp.2020.153225","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T22:55:20Z","timestamp":1600469720000},"page":"153225","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of digital image analysis on histological images of a murine embryoid body model for monitoring endothelial differentiation"],"prefix":"10.1016","volume":"216","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3696-8203","authenticated-orcid":false,"given":"Nabi","family":"Mousavi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie Birkvig","family":"Raft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9353-6389","authenticated-orcid":false,"given":"Sarah Line Bring","family":"Truelsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Timmermans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Thastrup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Heegaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.prp.2020.153225_bib0005","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/BF03401776","article-title":"Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers","volume":"6","author":"Itskovitz-Eldor","year":"2000","journal-title":"Mol. Med."},{"key":"10.1016\/j.prp.2020.153225_bib0010","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1038\/ng1668","article-title":"Current issues in mouse genome engineering","volume":"37","author":"Glaser","year":"2005","journal-title":"Nat. Genet."},{"key":"10.1016\/j.prp.2020.153225_bib0015","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/labinvest.3780252","article-title":"In vitro models of vasculogenesis and angiogenesis","volume":"81","author":"Vailhe","year":"2001","journal-title":"Lab. Invest."},{"key":"10.1016\/j.prp.2020.153225_bib0020","first-page":"473","article-title":"Hematopoietic commitment during embryonic stem cell differentiation in culture","volume":"13","author":"Keller","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.prp.2020.153225_bib0025","first-page":"27","article-title":"The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium","volume":"87","author":"Doetschman","year":"1985","journal-title":"J. Embryol. Exp. Morphol."},{"key":"10.1016\/j.prp.2020.153225_bib0030","first-page":"1301","article-title":"The embryoid body as a novel in vitro assay system for antiangiogenic agents","volume":"78","author":"Wartenberg","year":"1998","journal-title":"Lab. Invest."},{"key":"10.1016\/j.prp.2020.153225_bib0035","doi-asserted-by":"crossref","first-page":"3424","DOI":"10.1182\/blood.V88.9.3424.bloodjournal8893424","article-title":"Embryonic stem cells differentiate in vitro to endothelial cells through successive maturation steps","volume":"88","author":"Vittet","year":"1996","journal-title":"Blood"},{"key":"10.1016\/j.prp.2020.153225_bib0040","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1634\/stemcells.19-3-193","article-title":"Multilineage differentiation from human embryonic stem cell lines","volume":"19","author":"Odorico","year":"2001","journal-title":"Stem Cells"},{"key":"10.1016\/j.prp.2020.153225_bib0045","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1083\/jcb.200701146","article-title":"Building blood vessels--stem cell models in vascular biology","volume":"177","author":"Jakobsson","year":"2007","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.prp.2020.153225_bib0050","doi-asserted-by":"crossref","DOI":"10.1007\/s10278-020-00351-z","article-title":"Whole slide imaging (WSI) in pathology: current perspectives and future directions","author":"Kumar","year":"2020","journal-title":"J. Digit. Imaging"},{"key":"10.1016\/j.prp.2020.153225_bib0055","series-title":"Comparative Anatomy and Histology","first-page":"53","article-title":"4 - microscopy, immuno-histochemistry, digital imaging, and quantitative microscopy","author":"Goodwin","year":"2018"},{"key":"10.1016\/j.prp.2020.153225_bib0060","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1111\/his.13305","article-title":"Application of automated image analysis reduces the workload of manual screening of sentinel lymph node biopsies in breast cancer","volume":"71","author":"Holten-Rossing","year":"2017","journal-title":"Histopathology"},{"key":"10.1016\/j.prp.2020.153225_bib0065","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1242\/dev.102.3.471","article-title":"Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies","volume":"102","author":"Risau","year":"1988","journal-title":"Development"},{"key":"10.1016\/j.prp.2020.153225_bib0070","first-page":"995","article-title":"Tumor-induced angiogenesis studied in confrontation cultures of multicellular tumor spheroids and embryoid bodies grown from pluripotent embryonic stem cells","volume":"15","author":"Wartenberg","year":"2001","journal-title":"FASEB"},{"key":"10.1016\/j.prp.2020.153225_bib0075","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1242\/dev.114.2.303","article-title":"Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development","volume":"114","author":"Wang","year":"1992","journal-title":"Development"},{"key":"10.1016\/j.prp.2020.153225_bib0080","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1073\/pnas.032074999","article-title":"Endothelial cells derived from human embryonic stem cells","volume":"99","author":"Levenberg","year":"2002","journal-title":"Proc. Nat. Acad. Sci. U. S. A."},{"key":"10.1016\/j.prp.2020.153225_bib0085","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1177\/0192623315605933","article-title":"Utilizing whole slide images for pathology peer review and working groups","volume":"43","author":"Malarkey","year":"2015","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.prp.2020.153225_bib0090","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/s10238-015-0379-9","article-title":"Interval sentinel lymph nodes in melanoma: a digital pathology analysis of Ki67 expression and microvascular density","volume":"16","author":"Marinaccio","year":"2016","journal-title":"Clin. Exp. Med."},{"key":"10.1016\/j.prp.2020.153225_bib0095","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1177\/0300985813503570","article-title":"Whole-slide imaging and automated image analysis: considerations and opportunities in the practice of pathology","volume":"51","author":"Webster","year":"2014","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.prp.2020.153225_bib0100","doi-asserted-by":"crossref","first-page":"19163","DOI":"10.18632\/oncotarget.4383","article-title":"Continuous representation of tumor microvessel density and detection of angiogenic hotspots in histological whole-slide images","volume":"6","author":"Kather","year":"2015","journal-title":"Oncotarget"},{"key":"10.1016\/j.prp.2020.153225_bib0105","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1006\/mvre.1999.2233","article-title":"Quantitative assessment of angiogenesis and tumor vessel architecture by computer-assisted digital image analysis: effects of VEGF-toxin conjugate on tumor microvessel density","volume":"59","author":"Wild","year":"2000","journal-title":"Microvasc. Res."},{"key":"10.1016\/j.prp.2020.153225_bib0110","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s10456-004-8911-7","article-title":"Hanging-drop multicellular spheroids as a model of tumour angiogenesis","volume":"7","author":"Timmins","year":"2004","journal-title":"Angiogenesis"},{"key":"10.1016\/j.prp.2020.153225_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00418-014-1258-2","article-title":"Image analysis of immunohistochemistry is superior to visual scoring as shown for patient outcome of esophageal adenocarcinoma","volume":"143","author":"Feuchtinger","year":"2015","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.prp.2020.153225_bib0120","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1002\/ijc.29904","article-title":"Microvessel density and endothelial cell proliferation levels in colorectal liver metastases from patients given neo-adjuvant cytotoxic chemotherapy and bevacizumab","volume":"138","author":"Eefsen","year":"2016","journal-title":"Int. J. Cancer"}],"container-title":["Pathology - Research and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S034403382032080X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S034403382032080X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T06:47:39Z","timestamp":1759733259000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S034403382032080X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["S034403382032080X"],"URL":"https:\/\/doi.org\/10.1016\/j.prp.2020.153225","relation":{},"ISSN":["0344-0338"],"issn-type":[{"type":"print","value":"0344-0338"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Application of digital image analysis on histological images of a murine embryoid body model for monitoring endothelial differentiation","name":"articletitle","label":"Article Title"},{"value":"Pathology - Research and Practice","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.prp.2020.153225","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"153225"}}