{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T06:00:06Z","timestamp":1774418406120,"version":"3.50.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pathology - Research and Practice"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.prp.2024.155660","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T10:38:04Z","timestamp":1728988684000},"page":"155660","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Evaluating glycocalyx morphology and composition in frozen and formalin-fixed liver tumor sections"],"prefix":"10.1016","volume":"263","author":[{"given":"Masashi","family":"Kuno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahide","family":"Endo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Hara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Horaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoma","family":"Yokoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keita","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokatsu","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Fukada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chika","family":"Takao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Asai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itaru","family":"Yasufuku","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse Yu","family":"Tajima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Kiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhisa","family":"Matsuhashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.prp.2024.155660_bib1","doi-asserted-by":"crossref","first-page":"5415","DOI":"10.3390\/cancers14215415","article-title":"Glycocalyx acts as a central player in the development of tumor microenvironment by extracellular vesicles for angiogenesis and metastasis","volume":"14","author":"Zeng","year":"2022","journal-title":"Cancers"},{"key":"10.1016\/j.prp.2024.155660_bib2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13054-015-0741-z","article-title":"Glycocalyx and sepsis-induced alterations in vascular permeability","volume":"19","author":"Chelazzi","year":"2015","journal-title":"Crit. Care"},{"key":"10.1016\/j.prp.2024.155660_bib3","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1002\/path.3964","article-title":"Endothelial glycocalyx dysfunction in disease: albuminuria and increased microvascular permeability","volume":"226","author":"Salmon","year":"2012","journal-title":"J. Pathol."},{"key":"10.1016\/j.prp.2024.155660_bib4","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.trsl.2022.03.014","article-title":"Form follows function: the endothelial glycocalyx","volume":"247","author":"Suzuki","year":"2022","journal-title":"Transl. Res."},{"key":"10.1016\/j.prp.2024.155660_bib5","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.3390\/nu15112573","article-title":"Endothelial glycocalyx preservation-impact of nutrition and lifestyle","volume":"15","author":"Francekovi\u0107","year":"2023","journal-title":"Nutrients"},{"key":"10.1016\/j.prp.2024.155660_bib6","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nature13535","article-title":"The cancer glycocalyx mechanically primes integrin-mediated growth and survival","volume":"511","author":"Paszek","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.prp.2024.155660_bib7","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.201910070","article-title":"Don\u2019t sugarcoat it: how glycocalyx composition influences cancer progression","volume":"219","author":"Buffone","year":"2020","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.prp.2024.155660_bib8","doi-asserted-by":"crossref","first-page":"2966","DOI":"10.1111\/cas.14942","article-title":"Regulation of vascular permeability in cancer metastasis","volume":"112","author":"Tomita","year":"2021","journal-title":"Cancer Sci."},{"key":"10.1016\/j.prp.2024.155660_bib9","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.3390\/jcm8091270","article-title":"Human colorectal cancer infrastructure constructed by the glycocalyx","volume":"8","author":"Tachi","year":"2019","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.prp.2024.155660_bib10","article-title":"Glycocalyx analysis of bladder cancer: three-dimensional images in electron microscopy and Vicia villosa lectin as a marker for invasiveness in frozen sections","volume":"11","author":"Enomoto","year":"2023","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.prp.2024.155660_bib11","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.trsl.2022.03.014","article-title":"Form follows function: the endothelial glycocalyx","volume":"247","author":"Suzuki","year":"2022","journal-title":"Transl. Res."},{"key":"10.1016\/j.prp.2024.155660_bib12","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.1365-2761.2007.00846.x","article-title":"Characterization of glycans in the developmental stages of Myxobolus cerebralis (Myxozoa), the causative agent of whirling disease","volume":"30","author":"Kaltner","year":"2007","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.prp.2024.155660_bib13","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to imageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.prp.2024.155660_bib14","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/bmt.2012.244","article-title":"Investigation of the freely available easy-to-use software \u2018EZR\u2019 for medical statistics","volume":"48","author":"Kanda","year":"2013","journal-title":"Bone Marrow Transpl."},{"key":"10.1016\/j.prp.2024.155660_bib15","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1097\/00003226-198804000-00006","article-title":"Lectin binding patterns of the human cornea. Comparison of frozen and paraffin sections","volume":"7","author":"Brandon","year":"1988","journal-title":"Cornea"},{"issue":"67","key":"10.1016\/j.prp.2024.155660_bib16","doi-asserted-by":"crossref","DOI":"10.3791\/3928-v","article-title":"Using unfixed, frozen tissues to study natural mucin distribution","author":"Cohen","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.prp.2024.155660_bib17","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF00716182","article-title":"Carcinoembryonic antigen and lectin binding in the bile canalicular structures of hepatocellular carcinoma","volume":"412","author":"Machinami","year":"1987","journal-title":"Virchows Arch. A Pathol. Anat. Histopathol."},{"key":"10.1016\/j.prp.2024.155660_bib18","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF00490238","article-title":"Changes of glycoconjugates in human hepatocellular carcinoma","volume":"92","author":"Zhang","year":"1989","journal-title":"Histochemistry"},{"key":"10.1016\/j.prp.2024.155660_bib19","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/BF00747740","article-title":"Expression of glycoconjugates in intrahepatic cholangiocellular carcinoma","volume":"415","author":"Zhang","year":"1989","journal-title":"Virchows Arch. A Pathol. Anat. Histopathol."},{"key":"10.1016\/j.prp.2024.155660_bib20","first-page":"566","article-title":"Lectin binding patterns in normal liver, chronic active hepatitis, liver cirrhosis, and hepatocellular carcinoma. An immunohistochemical and immunoelectron microscopic study","volume":"42","author":"Murakami","year":"1992","journal-title":"Acta Pathol. Jpn."},{"key":"10.1016\/j.prp.2024.155660_bib21","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s10719-015-9645-z","article-title":"Alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV","volume":"33","author":"Qin","year":"2016","journal-title":"Glycoconj. J."},{"key":"10.1016\/j.prp.2024.155660_bib22","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1007\/BF02781922","article-title":"Histochemical properties of vascular and sinusoidal endothelial cells in liver diseases","volume":"26","author":"Hattori","year":"1991","journal-title":"Gastroenterol. Jpn."},{"key":"10.1016\/j.prp.2024.155660_bib23","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/BF01675686","article-title":"Proteolysis and lectin histochemistry","volume":"19","author":"Jeffrey","year":"1987","journal-title":"Histochem. J."},{"key":"10.1016\/j.prp.2024.155660_bib24","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF01682750","article-title":"The effects of various fixatives on subsequent lectin binding to tissue sections","volume":"19","author":"Allison","year":"1987","journal-title":"Histochem. J."},{"key":"10.1016\/j.prp.2024.155660_bib25","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/BF01002700","article-title":"Effects of histological processing on lectin binding patterns in oral mucosa and skin","volume":"15","author":"Rittman","year":"1983","journal-title":"Histochem. J."},{"key":"10.1016\/j.prp.2024.155660_bib26","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/BF00489534","article-title":"Lectin-binding spectra in the hyperplastic human tonsil. Effect of formalin fixation and paraffin embedding on lectin affinity of tissue components","volume":"81","author":"Wirbel","year":"1984","journal-title":"Histochemistry"},{"key":"10.1016\/j.prp.2024.155660_bib27","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3389\/fonc.2014.00028","article-title":"Altered tumor-cell glycosylation promotes metastasis","volume":"4","author":"H\u00e4uselmann","year":"2014","journal-title":"Front Oncol."},{"issue":"9","key":"10.1016\/j.prp.2024.155660_bib28","doi-asserted-by":"crossref","DOI":"10.1038\/nrc3982","article-title":"Glycosylation in cancer: mechanisms and clinical implications","volume":"15","author":"Pinho","year":"2015","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.prp.2024.155660_bib29","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1146\/annurev-pathol-012414-040438","article-title":"Protein glycosylation in cancer","volume":"10","author":"Stowell","year":"2015","journal-title":"Annu Rev. Pathol."}],"container-title":["Pathology - Research and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0344033824005715?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0344033824005715?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,23]],"date-time":"2024-11-23T03:54:53Z","timestamp":1732334093000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0344033824005715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":29,"alternative-id":["S0344033824005715"],"URL":"https:\/\/doi.org\/10.1016\/j.prp.2024.155660","relation":{},"ISSN":["0344-0338"],"issn-type":[{"value":"0344-0338","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluating glycocalyx morphology and composition in frozen and formalin-fixed liver tumor sections","name":"articletitle","label":"Article Title"},{"value":"Pathology - Research and Practice","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.prp.2024.155660","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"155660"}}