{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:29:30Z","timestamp":1792441770145,"version":"4.5.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Halchemix"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1016\/j.psj.2019.10.064","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T17:10:20Z","timestamp":1579713020000},"page":"1444-1453","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Egg production and quality responses to increasing isoleucine supplementation in Shaver white hens fed a low crude protein corn-soybean meal diet fortified with synthetic amino acids between 20 and 46\u00a0weeks of age"],"prefix":"10.1016","volume":"99","author":[{"given":"Ilona A.","family":"Parenteau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin","family":"Stevenson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah G.","family":"Kiarie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2019.10.064_bib1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1080\/00071660902951321","article-title":"Comparison of amino acid digestibility of feed ingredients in broilers, laying hens and caecectomised roosters","volume":"50","author":"Adedokun","year":"2009","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib2","doi-asserted-by":"crossref","first-page":"420","DOI":"10.3382\/ps.2013-03495","article-title":"Standardized ileal amino acid digestibility of meat and bone meal and soybean meal in laying hens and broilers","volume":"93","author":"Adedokun","year":"2014","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib3","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.3382\/ps\/pev226","article-title":"Standardized ileal amino acid digestibility of corn, corn distillers\u2019 dried grains with solubles, wheat middlings, and bakery by-products in broilers and laying hens","volume":"94","author":"Adedokun","year":"2015","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib4","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1080\/00071668.2016.1161727","article-title":"Increasing persistency in lay and stabilising egg quality in longer laying cycles. What are the challenges?","volume":"57","author":"Bain","year":"2016","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/ps\/81.4.485","article-title":"Ideal ratio (relative to lysine) of tryptophan, threonine, isoleucine, and valine for chicks during the second and third weeks posthatch","volume":"81","author":"Baker","year":"2002","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib6","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3382\/japr.2009-00093","article-title":"Supplementing l-valine and l-isoleucine in low-protein corn and soybean meal all-vegetable diets for broilers","volume":"19","author":"Berres","year":"2010","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2019.10.064_bib7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/japr\/8.1.25","article-title":"A quantitative assessment of reduced protein diets and supplements to improve nitrogen utilization","volume":"8","author":"Blair","year":"1999","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2019.10.064_bib8","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3382\/ps.0430396","article-title":"Studies with corn-soya laying diets: 7. Limiting amino acids in a 60:40 blend of corn and soybean protein","volume":"43","author":"Bray","year":"1964","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib9","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3382\/ps.0480674","article-title":"Studies with corn-soya laying diets 8. Requirements for limiting amino acids\u2014the basal diet and the requirements for isoleucine, lysine and tryptophan","volume":"48","author":"Bray","year":"1969","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib10","doi-asserted-by":"crossref","first-page":"744","DOI":"10.3382\/ps.2007-00412","article-title":"Ideal ratios of isoleucine, methionine, methionine plus cystine, threonine, tryptophan, and valine relative to lysine for white leghorn-type laying hens of twenty-eight to thirty-four weeks of age","volume":"87","author":"Bregendahl","year":"2008","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3382\/japr.2012-00609","article-title":"Effect of a reduced crude protein, amino acid-balanced diet on hen performance, production costs, and ammonia emissions in a commercial laying hen flock","volume":"22","author":"Burley","year":"2013","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2019.10.064_bib52","series-title":"CCAC guidelines on: the care and use of farm animals in research, teaching and testing","year":"2009"},{"key":"10.1016\/j.psj.2019.10.064_bib12","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S1516-635X2005000300007","article-title":"Commercial laying hen diets formulated according to different recommendations of total and digestible amino acids","volume":"7","author":"Casartelli","year":"2005","journal-title":"Braz. J. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib13","series-title":"Aminozurenbohoefte van leghennen en vleeskuikens [Amino Acid Requirements for Laying Hens and Broiler Chickens]","year":"1996"},{"key":"10.1016\/j.psj.2019.10.064_bib14","first-page":"13","article-title":"Ideal amino acid profile for layers examined","volume":"71","author":"Coon","year":"1999","journal-title":"Feedstuffs"},{"key":"10.1016\/j.psj.2019.10.064_bib15","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.3382\/ps.2009-00109","article-title":"Limitations of dietary isoleucine and valine in broiler chick diets1","volume":"88","author":"Corzo","year":"2009","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib53","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.3382\/ps\/pew163","article-title":"Effects of dietary L-isoleucine on laying performance and immunomodulation of laying hens","volume":"95","author":"Dong","year":"2016","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib18","series-title":"Proceedings of Intermountain Nutrition Conference","first-page":"193","article-title":"Amino acid nutrition of commercial pullets and layers","author":"Elliot","year":"2008"},{"key":"10.1016\/j.psj.2019.10.064_bib19","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1093\/japr\/2.3.273","article-title":"Performance of commercial laying hens fed various supplemental amino acids in a corn-soybean meal diet","volume":"2","author":"Harms","year":"1993","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2019.10.064_bib20","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.3382\/ps.0721892","article-title":"Optimizing egg mass with amino acid supplementation of a low-protein diet","volume":"72","author":"Harms","year":"1993","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib21","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1093\/ps\/79.8.1154","article-title":"Evaluation of the isoleucine requirement of the commercial layer in a corn-soybean meal diet","volume":"79","author":"Harms","year":"2000","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib51","series-title":"Nutrition Management Guide. 2011 ed","year":"2011"},{"key":"10.1016\/j.psj.2019.10.064_bib22","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/ps\/85.4.625","article-title":"Comparison of apparent ileal amino acid digestibility of feed ingredients measured with broilers, layers and roosters","volume":"85","author":"Huang","year":"2006","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib23","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1080\/00071669108417372","article-title":"Optimum isoleucine requirement of laying hens and the effect of age","volume":"32","author":"Huyghebaert","year":"1991","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib25","series-title":"Proceedings of the 26th Annual Northwest Animal Nutrition Conference. Henschel, K.K., ed","first-page":"159","article-title":"Protein and amino acid nutrition of laying hens and broilers","author":"Jensen","year":"1991"},{"key":"10.1016\/j.psj.2019.10.064_bib26","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3382\/japr.2013-00831","article-title":"Evaluation of amino-acid supplemented diets varying in protein levels for laying hens","volume":"23","author":"Ji","year":"2014","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2019.10.064_bib27","doi-asserted-by":"crossref","first-page":"905","DOI":"10.3382\/ps.0710905","article-title":"Performance of growing pullets and laying hens fed low-protein, amino acid-supplemented diets","volume":"71","author":"Keshavarz","year":"1992","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib28","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.3382\/ps.2011-01400","article-title":"Comparison of amino acid digestibility of feedstuffs determined with the precision-fed cecectomized rooster assay and the standardized ileal amino acid digestibility assay","volume":"90","author":"Kim","year":"2011","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib29","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.3382\/ps.2011-01861","article-title":"Comparison of amino acid digestibility coefficients for soybean meal, canola meal, fish meal, and meat and bone meal among 3 different bioassays","volume":"91","author":"Kim","year":"2012","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib30","doi-asserted-by":"crossref","first-page":"3141","DOI":"10.3382\/ps.2012-02418","article-title":"Comparison of amino acid digestibility coefficients for corn, corn gluten meal, and corn distillers dried grains with solubles among 3 different bioassays","volume":"91","author":"Kim","year":"2012","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib31","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1079\/WPS20000018","article-title":"Ammonia and poultry welfare: a review","volume":"56","author":"Kristensen","year":"2000","journal-title":"Worlds Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib32","series-title":"Commercial Poultry Production","author":"Leeson","year":"2005"},{"key":"10.1016\/j.psj.2019.10.064_bib33","doi-asserted-by":"crossref","first-page":"842","DOI":"10.3382\/ps\/pev380","article-title":"Maintenance valine, isoleucine and tryptophan requirements for poultry","volume":"95","author":"de Lima","year":"2016","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.anifeedsci.2018.02.001","article-title":"The optimal digestible valine, isoleucine and tryptophan intakes of broiler breeder hens for rate of lay","volume":"238","author":"de Lima","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.1016\/j.psj.2019.10.064_bib35","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1590\/S1516-35982012000500032","article-title":"Determination of digestible isoleucine: lysine ratio in diets for laying hens aged 42-58 weeks","volume":"41","author":"Mello","year":"2012","journal-title":"Rev. Bras. Zootec."},{"key":"10.1016\/j.psj.2019.10.064_bib36","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/00071660120048474","article-title":"Nitrogen retention and performance of brown laying hens on diets with different protein content and constant concentration of amino acids and energy","volume":"42","author":"Meluzzi","year":"2001","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib37","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.3382\/ps.0331201","article-title":"The isoleucine requirements of the laying hen","volume":"33","author":"Miller","year":"1954","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib38","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/00071668808417030","article-title":"Partitioning of the response to protein between egg number and egg weight","volume":"29","author":"Morris","year":"1988","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib39","doi-asserted-by":"crossref","first-page":"392","DOI":"10.2141\/jpsa.0140022","article-title":"Influence of dietary calcium concentration on the digestion of nutrients along the intestinal tract of broiler chickens","volume":"51","author":"Mutucumarana","year":"2014","journal-title":"J. Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib40","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.3382\/ps\/pey118","article-title":"Egg production and quality responses of adding up to 7.5% defatted black soldier fly larvae meal in a corn\u2013soybean meal diet fed to Shaver White Leghorns from wk 19 to 27 of age","volume":"97","author":"Mwaniki","year":"2018","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib41","series-title":"Nutrient Requirements of Poultry","year":"1994"},{"key":"10.1016\/j.psj.2019.10.064_bib42","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.5433\/1679-0359.2015v36n3p1735","article-title":"Tryptophan, threonine and isoleucine supplementation in low-protein diets for commercial laying hens","volume":"36","author":"Ospina Rojas","year":"2015","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"10.1016\/j.psj.2019.10.064_bib43","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1093\/ps\/82.5.815","article-title":"Requirement of tryptophan in relation to the supply of large neutral amino acids in laying hens","volume":"82","author":"Peganova","year":"2003","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib44","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1093\/ps\/86.8.1716","article-title":"Effects of dietary fiber and reduced crude protein on nitrogen balance and egg production in laying hens","volume":"86","author":"Roberts","year":"2007","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib45","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1590\/S1516-35982013001100003","article-title":"Ideal digestible isoleucine: digestible lysine ratio in diets for laying hens aged 24-40 weeks","volume":"42","author":"Rocha","year":"2013","journal-title":"Rev. Bras. Zootec."},{"key":"10.1016\/j.psj.2019.10.064_bib46","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1093\/ps\/81.12.1869","article-title":"Re-evaluation of the isoleucine requirement of the commercial layer","volume":"81","author":"Shivazad","year":"2002","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib47","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1093\/ps\/81.7.1038","article-title":"Influence of supplemental lysine, isoleucine, threonine, tryptophan and total sulfur amino acids on egg weight of Hyline W-36 hens","volume":"81","author":"Sohail","year":"2002","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib48","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.3382\/ps.0721473","article-title":"Reducing nitrogen excretion of the laying hen by feeding lower crude protein diets","volume":"72","author":"Summers","year":"1993","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2019.10.064_bib49","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s00484-014-0870-0","article-title":"Response of laying hens to feeding low-protein amino acid-supplemented diets under high ambient temperature: performance, egg quality, leukocyte profile, blood lipids and excreta pH","volume":"59","author":"Torki","year":"2015","journal-title":"Int. J. Biometeorol."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119577793?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119577793?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T22:24:45Z","timestamp":1758493485000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579119577793"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["S0032579119577793"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2019.10.064","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Egg production and quality responses to increasing isoleucine supplementation in Shaver white hens fed a low crude protein corn-soybean meal diet fortified with synthetic amino acids between 20 and 46\u00a0weeks of age","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2019.10.064","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}]}}