{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:27:31Z","timestamp":1790281651009,"version":"4.1.0"},"reference-count":27,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Vetagro S.p.A."}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.psj.2020.07.035","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T21:23:21Z","timestamp":1597181001000},"page":"5350-5355","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["In\u00a0vitro anticoccidial activity of thymol, carvacrol, and saponins"],"prefix":"10.1016","volume":"99","author":[{"given":"Martina","family":"Felici","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Tugnoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Ghiselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Massi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Tosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Fiorentini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Piva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Grilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2020.07.035_bib1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1017\/S0043933912000268","article-title":"Botanicals: an alternative approach for the control of avian coccidiosis","volume":"68","author":"Abbas","year":"2012","journal-title":"Worlds Poult. Sci. J."},{"key":"10.1016\/j.psj.2020.07.035_bib2","doi-asserted-by":"crossref","first-page":"3913","DOI":"10.1007\/s00436-015-4637-2","article-title":"In\u00a0vitro efficacy of allicin on chicken Eimeria tenella sporozoites","volume":"114","author":"Alnassan","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.psj.2020.07.035_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0020-7519(00)00150-8","article-title":"Cell: sporozoite interactions and invasion by apicomplexan parasites of the genus Eimeria","volume":"31","author":"Augustine","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.psj.2020.07.035_bib4","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1550-7408.2001.tb00300.x","article-title":"Invasion of different cell types by sporozoites of Eimeria species and effects of Monoclonal Antibodv 1209-C2 on invasion of cells bv sporozoites of several apicomplexan parasites","volume":"48","author":"Augustine","year":"2001","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.psj.2020.07.035_bib5","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1080\/00071668.2013.849795","article-title":"An update on approaches to controlling coccidia in poultry using botanical extracts","volume":"54","author":"Bozkurt","year":"2013","journal-title":"Br. Poult. Sci."},{"key":"10.1016\/j.psj.2020.07.035_bib6","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.vetpar.2012.09.001","article-title":"In\u00a0vitro inhibition of Eimeria tenella invasion of epithelial cells by phytochemicals","volume":"191","author":"Burt","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2020.07.035_bib7","series-title":"Farm Animals Disease. Recent Omic Trends and New Strategies of Treatment","first-page":"135","article-title":"Natural compounds as an alternative to control farm diseases: avian coccidiosis","author":"Cobaxin-C\u00e1rdenas","year":"2018"},{"key":"10.1016\/j.psj.2020.07.035_bib8","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.meatsci.2004.04.010","article-title":"Detection of meat species using TaqMan real-time PCR assays","volume":"68","author":"Dooley","year":"2004","journal-title":"Meat Sci."},{"key":"10.1016\/j.psj.2020.07.035_bib9","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1079\/BJN2002725","article-title":"The biological action of saponins in animal systems: a review","volume":"88","author":"Francis","year":"2002","journal-title":"Br. J. Nutr."},{"key":"10.1016\/j.psj.2020.07.035_bib10","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/0003942031000107299","article-title":"Effect of dietary supplementation with oregano essential oil on performance of broilers after experimental infection with Eimeria tenella","volume":"57","author":"Giannenas","year":"2003","journal-title":"Arch. Anim. Nutr."},{"key":"10.1016\/j.psj.2020.07.035_bib11","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.vetpar.2016.08.020","article-title":"In\u00a0vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenella","volume":"228","author":"Jitviriyanon","year":"2016","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2020.07.035_bib12","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1637\/8351-050908-Reg.1","article-title":"Detection of five avian Eimeria species by species-specific real-time Polymerase Chain reaction assay","volume":"52","author":"Kawahara","year":"2008","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2020.07.035_bib13","first-page":"1","article-title":"Effects of vaccination against coccidiosis, with and without a specific herbal essential oil blend, on performance, oocyst excretion and serum IBD titers of broilers reared on litter","volume":"11","author":"K\u00fc\u00e7\u00fckyilmaz","year":"2012","journal-title":"Ital. J. Anim. Sci."},{"key":"10.1016\/j.psj.2020.07.035_bib14","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.psj.2020.07.035_bib15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/03079457.2016.1150587","article-title":"Necrotic enteritis predisposing factors in broiler chickens","volume":"45","author":"Moore","year":"2016","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2020.07.035_bib16","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.3390\/ph6121451","article-title":"Effect of essential oils on pathogenic Bacteria","volume":"6","author":"Nazzaro","year":"2013","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.psj.2020.07.035_bib17","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/01652176.2011.605247","article-title":"Coccidiosis in poultry: anticoccidial products, vaccines and other prevention strategies","volume":"31","author":"Peek","year":"2011","journal-title":"Vet. Q."},{"key":"10.1016\/j.psj.2020.07.035_bib18","doi-asserted-by":"crossref","first-page":"430610","DOI":"10.1155\/2015\/430610","article-title":"Control of avian coccidiosis: future and present natural alternatives","volume":"2015","author":"Quiroz-Casta\u00f1eda","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.psj.2020.07.035_bib19","series-title":"The Principles of Humane Experimental Technique","author":"Russel","year":"1959"},{"key":"10.1016\/j.psj.2020.07.035_bib20","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4014\/jmb.1608.08024","article-title":"Antimicrobial activity of basil, oregano, and thyme essential oils","volume":"27","author":"Sakkas","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.psj.2020.07.035_bib21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s10974-007-9113-x","article-title":"Effect of natural phenol derivatives on skeletal type sarcoplasmic reticulum Ca2+-ATPase and ryanodine receptor","volume":"28","author":"S\u00e1rk\u00f6zi","year":"2007","journal-title":"J. Muscle Res. Cell Motil."},{"key":"10.1016\/j.psj.2020.07.035_bib22","series-title":"Guidelines on Techniques in Coccidiosis Research","first-page":"1","article-title":"Eimeria species and strains of chickens","author":"Shirley","year":"1995"},{"key":"10.1016\/j.psj.2020.07.035_bib23","doi-asserted-by":"crossref","first-page":"13","DOI":"10.9790\/2380-07111317","article-title":"Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers","volume":"7","author":"Siddiki","year":"2014","journal-title":"IOSR J. Agric. Vet. Sci."},{"key":"10.1016\/j.psj.2020.07.035_bib24","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1177\/0394632016671728","article-title":"Critical evaluation of challenges and future use of animals in experimentation for biomedical research","volume":"29","author":"Singh","year":"2016","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"10.1016\/j.psj.2020.07.035_bib25","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.vetpar.2016.12.005","article-title":"Anticoccidial efficacy testing: in\u00a0vitro Eimeria tenella assays as replacement for animal experiments","volume":"233","author":"Thabet","year":"2017","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2020.07.035_bib26","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s00436-003-0846-1","article-title":"Comparative development of Eimeria tenella (Apicomplexa) in host cells in\u00a0vitro","volume":"90","author":"Tierney","year":"2003","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.psj.2020.07.035_bib27","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.parint.2014.01.006","article-title":"Detection of four important Eimeria species by multiplex PCR in a single assay","volume":"63","author":"You","year":"2014","journal-title":"Parasitol. Int."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579120305083?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579120305083?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T22:32:35Z","timestamp":1758493955000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579120305083"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":27,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["S0032579120305083"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2020.07.035","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0vitro anticoccidial activity of thymol, carvacrol, and saponins","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2020.07.035","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}]}}