{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:08:38Z","timestamp":1792796918090,"version":"4.5.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1016\/j.psj.2023.103204","type":"journal-article","created":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T09:54:16Z","timestamp":1697363656000},"page":"103204","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immune synergistic mechanism of recombinant plasmid adjuvant containing chicken IL-4 and IL-2 fusion genes on chicken coccidia live vaccine"],"prefix":"10.1016","volume":"103","author":[{"given":"Fan","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longlong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhen","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2249-0592","authenticated-orcid":false,"given":"Mingxue","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2023.103204_bib0001","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1093\/ps\/76.8.1150","article-title":"Assessment of recombinant bovine somatotropin as an immunomodulator during avian coccidiosis: immunization with living oocysts","volume":"76","author":"Allen","year":"1997","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0002","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1471-2164-13-21","article-title":"Characterisation of full-length cDNA sequences provides insights into the Eimeria tenella transcriptome","volume":"13","author":"Amiruddin","year":"2012","journal-title":"BMC Genom."},{"key":"10.1016\/j.psj.2023.103204_bib0003","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.3390\/genes11091103","article-title":"Exploring Eimeria genomes to understand population biology: recent progress and future opportunities","volume":"11","author":"Blake","year":"2020","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.psj.2023.103204_bib0004","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-3024.1997.d01-191.x","article-title":"Peripheral blood lymphocytes from Eimeria tenella infected chickens produce gamma-interferon after stimulation in vitro","volume":"19","author":"Breed","year":"1997","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.psj.2023.103204_bib0005","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.ijpddr.2014.10.002","article-title":"Vaccination of chickens against coccidiosis ameliorates drug resistance in commercial poultry production","volume":"4","author":"Chapman","year":"2014","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.psj.2023.103204_bib0006","doi-asserted-by":"crossref","first-page":"7204","DOI":"10.18632\/oncotarget.23208","article-title":"Inflammatory responses and inflammation-associated diseases in organs","volume":"9","author":"Chen","year":"2018","journal-title":"Oncotarget"},{"key":"10.1016\/j.psj.2023.103204_bib0007","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2009.02.001","article-title":"Host response to simultaneous infections with Eimeria acervulina, maxima and tenella: a cumulation of single responses","volume":"162","author":"Cornelissen","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2023.103204_bib0008","doi-asserted-by":"crossref","first-page":"3818","DOI":"10.1016\/j.vaccine.2011.03.022","article-title":"Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC-derived exosomes","volume":"29","author":"Del Cacho","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.psj.2023.103204_bib0009","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.vetmic.2019.01.008","article-title":"Adjuvant effects of interleukin-2 co-expression with VP60 in an oral vaccine delivered by attenuated Salmonella typhimurium against rabbit hemorrhagic disease","volume":"230","author":"Deng","year":"2019","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.psj.2023.103204_bib0010","doi-asserted-by":"crossref","first-page":"56","DOI":"10.3382\/ps.2007-00328","article-title":"Hematology and enzyme histochemistry of the peripheral blood leucocytes in rock partridges (Alectoris graeca)","volume":"87","author":"Donmez","year":"2008","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0011","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S1074-7613(02)00471-5","article-title":"IFN-gamma represses IL-4 expression via IRF-1 and IRF-2","volume":"17","author":"Elser","year":"2002","journal-title":"Immunity"},{"key":"10.1016\/j.psj.2023.103204_bib0012","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.1016\/j.psj.2020.08.022","article-title":"Interleukin-2 shows high adjuvanticity for an inactivated vaccine against duck Tembusu virus disease","volume":"99","author":"Gao","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0013","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1093\/ps\/83.7.1124","article-title":"Effects of mushroom and herb polysaccharides on cellular and humoral immune responses of Eimeria tenella-infected chickens","volume":"83","author":"Guo","year":"2004","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0014","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.1007\/s00436-017-5636-2","article-title":"Experimental Toxoplasma gondii and Eimeria tenella co-infection in chickens","volume":"116","author":"Hiob","year":"2017","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.psj.2023.103204_bib0015","doi-asserted-by":"crossref","first-page":"476","DOI":"10.3390\/v11050476","article-title":"Co-expression of chicken IL-2 and IL-7 enhances the immunogenicity and protective efficacy of a VP2-expressing DNA vaccine against IBDV in chickens","volume":"11","author":"Huo","year":"2019","journal-title":"Viruses"},{"key":"10.1016\/j.psj.2023.103204_bib0016","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.intimp.2012.08.009","article-title":"Role of interplay between IL-4 and IFN-gamma in the in regulating M1 macrophage polarization induced by Nattectin","volume":"14","author":"Ishizuka","year":"2012","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.psj.2023.103204_bib0017","first-page":"18","article-title":"Synergistic effect of recombinant fusion gene adjuvant of chIL-4 and chlL-2 on chicken coccidiosis live vaccine","volume":"645","author":"Kailing","year":"2022","journal-title":"Heilongjiang Anim. Sci. Vet. Med."},{"key":"10.1016\/j.psj.2023.103204_bib0018","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.vetpar.2015.11.011","article-title":"Effect of Acetamizuril on enolase in second-generation merozoites of Eimeria tenella","volume":"215","author":"Liu","year":"2016","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2023.103204_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2021.105116","article-title":"Evaluation of chickens infected with a recombinant virulent NDV clone expressing chicken IL4","volume":"159","author":"Marcano","year":"2021","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.psj.2023.103204_bib0020","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0213911","article-title":"Induction and maintenance of bi-functional (IFN-gamma\u00a0+\u00a0IL-2+ and IL-2+ TNF-alpha+) T cell responses by DNA prime MVA boosted subtype C prophylactic vaccine tested in a Phase I trial in India","volume":"14","author":"Munusamy Ponnan","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.psj.2023.103204_bib0021","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101549","article-title":"Glycyrrhiza polysaccharides can improve and prolong the response of chickens to the Newcastle disease vaccine","volume":"101","author":"Wu","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0022","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.101791","article-title":"Poly (lactic-co-glycolic acid) nanoparticle-based vaccines delivery systems as a novel adjuvant for H9N2 antigen enhance immune responses","volume":"101","author":"Wusiman","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0023","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.beha.2016.08.028","article-title":"Clonal B-cell lymphocytosis of marginal zone origin","volume":"30","author":"Xochelli","year":"2017","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"10.1016\/j.psj.2023.103204_bib0024","doi-asserted-by":"crossref","first-page":"32679","DOI":"10.1073\/pnas.2018497117","article-title":"IL-4\/STAT6 signaling facilitates innate hematoma resolution and neurological recovery after hemorrhagic stroke in mice","volume":"117","author":"Xu","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.psj.2023.103204_bib0025","first-page":"954","article-title":"Cytokines IL-2,IL-12 and IL-18 enhance the protective immunity of ESAT-6 DNA vaccine against Mycobacterium tuberculosis","volume":"35","author":"Yin","year":"2019","journal-title":"Immunol. J."},{"key":"10.1016\/j.psj.2023.103204_bib0027","doi-asserted-by":"crossref","first-page":"859","DOI":"10.2307\/1592424","article-title":"In vivo role of tumor necrosis-like factor in Eimeria tenella infection","volume":"39","author":"Zhang","year":"1995","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2023.103204_bib0026","doi-asserted-by":"crossref","first-page":"4384","DOI":"10.3382\/ps\/pez218","article-title":"Comparison of the host cells apoptosis induced by precocious strains and virulent strains of Eimeria tenella","volume":"98","author":"Zhang","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2023.103204_bib0028","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.102530","article-title":"Prevention and control of chicken coccidiosis construction of recombinant Lactococcus lactis expressing chicken IL-4 and IL-2 fusion protein and its immune synergistic effect on chicken coccidia live vaccine","volume":"102","author":"Zheng","year":"2023","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003257912300723X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003257912300723X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T22:54:30Z","timestamp":1758495270000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S003257912300723X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["S003257912300723X"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2023.103204","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immune synergistic mechanism of recombinant plasmid adjuvant containing chicken IL-4 and IL-2 fusion genes on chicken coccidia live vaccine","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2023.103204","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"103204"}}