{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T07:28:03Z","timestamp":1793604483787,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012659","name":"Foundation for Innovative Research Groups of the National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.psj.2024.103482","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T19:06:50Z","timestamp":1706123210000},"page":"103482","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":36,"title":["Sodium butyrate alleviates free fatty acid-induced steatosis in primary chicken hepatocytes via the AMPK\/PPAR\u03b1 pathway"],"prefix":"10.1016","volume":"103","author":[{"given":"Jiayi","family":"Ding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuyue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huabin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoquan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2024.103482_bib0001","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jnutbio.2019.05.006","article-title":"Gamma-glutamyl carboxylated Gas6 mediates the beneficial effect of vitamin K on lowering hyperlipidemia via regulating the AMPK\/SREBP1\/PPAR\u03b1 signaling cascade of lipid metabolism","volume":"70","author":"Bordoloi","year":"2019","journal-title":"J. Nutr. Biochem."},{"key":"10.1016\/j.psj.2024.103482_bib0002","doi-asserted-by":"crossref","first-page":"23786","DOI":"10.1074\/jbc.273.37.23786","article-title":"Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor alpha","volume":"273","author":"Brandt","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0003","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1038\/ijo.2015.84","article-title":"The role of short chain fatty acids in appetite regulation and energy homeostasis","volume":"39","author":"Byrne","year":"2015","journal-title":"Int. J. Obes. (Lond.)"},{"key":"10.1016\/j.psj.2024.103482_bib0004","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1073\/pnas.1322269111","article-title":"The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition","volume":"111","author":"Chang","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.psj.2024.103482_bib0005","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.canlet.2019.11.019","article-title":"Clostridium butyricum, a butyrate-producing probiotic, inhibits intestinal tumor development through modulating Wnt signaling and gut microbiota","volume":"469","author":"Chen","year":"2020","journal-title":"Cancer Lett."},{"key":"10.1016\/j.psj.2024.103482_bib0006","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.bbrc.2009.11.029","article-title":"Molecular mechanism for the regulation of human ACC2 through phosphorylation by AMPK","volume":"391","author":"Cho","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.psj.2024.103482_bib0007","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.cmet.2011.02.018","article-title":"The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon","volume":"13","author":"Donohoe","year":"2011","journal-title":"Cell. Metab."},{"key":"10.1016\/j.psj.2024.103482_bib0008","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.997018","article-title":"Gut dysbiosis in nonalcoholic fatty liver disease: pathogenesis, diagnosis, and therapeutic implications","volume":"12","author":"Fang","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"Suppl 2","key":"10.1016\/j.psj.2024.103482_bib0009","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1463-1326.2010.01275.x","article-title":"Hepatic steatosis: a role for de novo lipogenesis and the transcription factor SREBP-1c","volume":"12","author":"Ferr\u00e9","year":"2010","journal-title":"Diabetes Obes. Metab."},{"key":"10.1016\/j.psj.2024.103482_bib0010","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.3382\/ps\/pey586","article-title":"Effects of fatty liver hemorrhagic syndrome on the AMP-activated protein kinase signaling pathway in laying hens","volume":"98","author":"Gao","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.103482_bib0011","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2021.590638","article-title":"Serum metabolomic profiling to reveal potential biomarkers for the diagnosis of fatty liver hemorrhagic syndrome in laying hens","volume":"12","author":"Guo","year":"2021","journal-title":"Front. Physiol."},{"key":"10.1016\/j.psj.2024.103482_bib0012","doi-asserted-by":"crossref","first-page":"28918","DOI":"10.1074\/jbc.M910350199","article-title":"Defect in peroxisome proliferator-activated receptor alpha-inducible fatty acid oxidation determines the severity of hepatic steatosis in response to fasting","volume":"275","author":"Hashimoto","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0013","doi-asserted-by":"crossref","first-page":"13470","DOI":"10.1074\/jbc.270.22.13470","article-title":"Mode of action of peroxisome proliferators as hypolipidemic drugs. Suppression of apolipoprotein C-III","volume":"270","author":"Hertz","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0014","doi-asserted-by":"crossref","first-page":"56071","DOI":"10.18632\/oncotarget.11267","article-title":"Butyrate alleviates high fat diet-induced obesity through activation of adiponectin-mediated pathway and stimulation of mitochondrial function in the skeletal muscle of mice","volume":"7","author":"Hong","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.psj.2024.103482_bib0015","series-title":"The investigation on relationship between AMPK signal pathway in lipid metabolism in primary hepatocytes from laying hens","author":"Huang","year":"2019"},{"key":"10.1016\/j.psj.2024.103482_bib0016","doi-asserted-by":"crossref","first-page":"825","DOI":"10.3390\/metabo12090825","article-title":"Inhibition of hepatic AMPK pathway contributes to free fatty acids-induced fatty liver disease in laying hen","volume":"12","author":"Huang","year":"2022","journal-title":"Metabolites"},{"key":"10.1016\/j.psj.2024.103482_bib0017","first-page":"1677","article-title":"Butyrate stimulates hepatic gluconeogenesis in mouse primary hepatocytes","volume":"17","author":"Ji","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"10.1016\/j.psj.2024.103482_bib0018","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.cmet.2017.07.009","article-title":"Acetyl CoA carboxylase inhibition reduces hepatic steatosis but elevates plasma triglycerides in mice and humans: a bedside to bench investigation","volume":"26","author":"Kim","year":"2017","journal-title":"Cell. Metab."},{"key":"10.1016\/j.psj.2024.103482_bib0019","doi-asserted-by":"crossref","first-page":"8030","DOI":"10.1073\/pnas.1016088108","article-title":"Short-chain fatty acids and ketones directly regulate sympathetic nervous system via G protein-coupled receptor 41 (GPR41)","volume":"108","author":"Kimura","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.psj.2024.103482_bib0020","doi-asserted-by":"crossref","first-page":"9520","DOI":"10.1074\/jbc.M111421200","article-title":"Diminished hepatic response to fasting\/refeeding and liver X receptor agonists in mice with selective deficiency of sterol regulatory element-binding protein-1c","volume":"277","author":"Liang","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0021","article-title":"Butyrate and propionate protect against diet-induced obesity and regulate gut hormones via free fatty acid receptor 3-independent mechanisms","volume":"7","author":"Lin","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.psj.2024.103482_bib0022","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s10020-020-00227-6","article-title":"Hepatocyte high-mobility group box 1 protects against steatosis and cellular stress during high fat diet feeding","volume":"26","author":"Lin","year":"2020","journal-title":"Mol. Med."},{"key":"10.1016\/j.psj.2024.103482_bib0023","article-title":"Protective effects of Abrus cantoniensis Hance on the fatty liver hemorrhagic syndrome in laying hens based on liver metabolomics and gut microbiota","volume":"9","author":"Liu","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2024.103482_bib0024","doi-asserted-by":"crossref","first-page":"5209","DOI":"10.3390\/ijms23095209","article-title":"Chronic intermittent hypoxia exposure alternative to exercise alleviates high-fat-diet-induced obesity and fatty liver","volume":"23","author":"Luo","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.psj.2024.103482_bib0025","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.3389\/fimmu.2018.01832","article-title":"Butyrate and dietary soluble fiber improve neuroinflammation associated with aging in mice","volume":"9","author":"Matt","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.psj.2024.103482_bib0026","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101320","article-title":"Untargeted and targeted metabolomics profiling reveals the underlying pathogenesis and abnormal arachidonic acid metabolism in laying hens with fatty liver hemorrhagic syndrome","volume":"100","author":"Meng","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.103482_bib0027","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/j.1432-1033.1988.tb14201.x","article-title":"Identification by amino acid sequencing of three major regulatory phosphorylation sites on rat acetyl-CoA carboxylase","volume":"175","author":"Munday","year":"1988","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.psj.2024.103482_bib0028","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/nature12656","article-title":"A small-molecule AdipoR agonist for type 2 diabetes and short life in obesity","volume":"503","author":"Okada-Iwabu","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.psj.2024.103482_bib0029","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1093\/ajcn\/nqac217","article-title":"The human gut microbiota and glucose metabolism: a scoping review of key bacteria and the potential role of SCFAs","volume":"116","author":"Palmn\u00e4s-B\u00e9dard","year":"2022","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.psj.2024.103482_bib0030","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.jhep.2014.10.039","article-title":"Molecular mechanism of PPAR\u03b1 action and its impact on lipid metabolism, inflammation and fibrosis in non-alcoholic fatty liver disease","volume":"62","author":"Pawlak","year":"2015","journal-title":"J. Hepatol."},{"key":"10.1016\/j.psj.2024.103482_bib0031","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature11450","article-title":"A metagenome-wide association study of gut microbiota in type 2 diabetes","volume":"490","author":"Qin","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.psj.2024.103482_bib0032","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1111\/j.1440-1746.2008.05733.x","article-title":"Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes","volume":"24","author":"Ricchi","year":"2009","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.psj.2024.103482_bib0033","doi-asserted-by":"crossref","first-page":"9453","DOI":"10.3390\/nu7115475","article-title":"The subtle balance between lipolysis and lipogenesis: a critical point in metabolic homeostasis","volume":"7","author":"Saponaro","year":"2015","journal-title":"Nutrients"},{"key":"10.1016\/j.psj.2024.103482_bib0034","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.1002\/j.1460-2075.1996.tb00918.x","article-title":"PPARalpha and PPARgamma activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene","volume":"15","author":"Schoonjans","year":"1996","journal-title":"EMBO J."},{"key":"10.1016\/j.psj.2024.103482_bib0035","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1126\/science.1120781","article-title":"The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin","volume":"310","author":"Shaw","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.psj.2024.103482_bib0036","doi-asserted-by":"crossref","first-page":"27029","DOI":"10.1074\/jbc.M202638200","article-title":"The role of SREBP-1c in nutritional regulation of lipogenic enzyme gene expression","volume":"277","author":"Stoeckman","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0037","series-title":"Effect of butyrate on alleviating high-fat diet-induced NAFLD and its mechanisms in rats","author":"Sun","year":"2018"},{"key":"10.1016\/j.psj.2024.103482_bib0038","doi-asserted-by":"crossref","first-page":"7633","DOI":"10.1021\/acs.jafc.8b01189","article-title":"Sodium butyrate ameliorates high-fat-diet-induced non-alcoholic fatty liver disease through peroxisome proliferator-activated receptor \u03b1-mediated activation of \u03b2 oxidation and suppression of inflammation","volume":"66","author":"Sun","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0039","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s10528-020-09971-0","article-title":"Suppression of miR-30a-3p attenuates hepatic steatosis in non-alcoholic fatty liver disease","volume":"58","author":"Wang","year":"2020","journal-title":"Biochem. Genet."},{"key":"10.1016\/j.psj.2024.103482_bib0040","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12944-017-0464-z","article-title":"Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1\/AMPK\u03b1\/SREBP1 signaling pathway","volume":"16","author":"Wang","year":"2017","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.psj.2024.103482_bib0041","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/03079457.2019.1698712","article-title":"Abnormal expression of liver autophagy and apoptosis-related mRNA in fatty liver haemorrhagic syndrome and improvement function of resveratrol in laying hens","volume":"49","author":"Wang","year":"2020","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2024.103482_bib0042","doi-asserted-by":"crossref","first-page":"50237","DOI":"10.1074\/jbc.M201208200","article-title":"Peroxisome proliferator-activated receptor alpha (PPARalpha) influences substrate utilization for hepatic glucose production","volume":"277","author":"Xu","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2024.103482_bib0043","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s40104-023-00900-w","article-title":"Ginkgo biloba extract alleviates fatty liver hemorrhagic syndrome in laying hens via reshaping gut microbiota","volume":"14","author":"Yang","year":"2023","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.psj.2024.103482_bib0044","doi-asserted-by":"crossref","first-page":"27572","DOI":"10.1038\/srep27572","article-title":"Accelerated dysbiosis of gut microbiota during aggravation of DSS-induced colitis by a butyrate-producing bacterium","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.psj.2024.103482_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2021.112029","article-title":"Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPAR\u03b1\/SREBP-1c\/FAS\/GPAT\/AMPK signal pathway","volume":"149","author":"Zhang","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.psj.2024.103482_bib0046","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/j.jcmgh.2021.05.006","article-title":"Sodium butyrate supplementation inhibits hepatic steatosis by stimulating liver kinase B1 and insulin-induced gene","volume":"12","author":"Zhao","year":"2021","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.psj.2024.103482_bib0047","article-title":"Gut microbiota and serum metabolite potential interactions in growing layer hens exposed to high-ambient temperature","volume":"9","author":"Zhou","year":"2022","journal-title":"Front. Nutr."},{"key":"10.1016\/j.psj.2024.103482_bib0048","doi-asserted-by":"crossref","first-page":"10141","DOI":"10.1038\/s41598-019-46183-y","article-title":"Insulin resistance and metabonomics analysis of fatty liver haemorrhagic syndrome in laying hens induced by a high-energy low-protein diet","volume":"9","author":"Zhuang","year":"2019","journal-title":"Sci. Rep."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124000610?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124000610?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T23:00:49Z","timestamp":1758495649000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579124000610"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S0032579124000610"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2024.103482","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sodium butyrate alleviates free fatty acid-induced steatosis in primary chicken hepatocytes via the AMPK\/PPAR\u03b1 pathway","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2024.103482","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"103482"}}