{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:15:56Z","timestamp":1788401756833,"version":"build-2803163510"},"reference-count":18,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T00:00:00Z","timestamp":1710720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA NIFA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.psj.2024.103681","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T23:01:43Z","timestamp":1711062103000},"page":"103681","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of real-time PCR assay for quantitative detection of Clostridium septicum"],"prefix":"10.1016","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6308-0878","authenticated-orcid":false,"given":"Bishnu","family":"Adhikari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2416-2747","authenticated-orcid":false,"given":"Guillermo","family":"Tellez-Isaias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tieshan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Wooming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Min","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2024.103681_bib0001","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2020.596933","article-title":"Comprehensive survey of the litter bacterial communities in commercial turkey farms","volume":"7","author":"Adhikari","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2024.103681_bib0002","doi-asserted-by":"crossref","first-page":"12296","DOI":"10.1021\/jf304690e","article-title":"Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues","volume":"60","author":"Cao","year":"2012","journal-title":"J. Agric. Food Chem"},{"key":"10.1016\/j.psj.2024.103681_bib0003","doi-asserted-by":"crossref","first-page":"736","DOI":"10.2307\/1592289","article-title":"Excessive mortality in market-age turkeys associated with cellulitis","volume":"40","author":"Carr","year":"1996","journal-title":"Avian Dis"},{"key":"10.1016\/j.psj.2024.103681_bib0004","doi-asserted-by":"crossref","first-page":"299","DOI":"10.2174\/156652408784533751","article-title":"The effects of physical and psychological stress on the gastrointestinal tract: Lessons from animal models","volume":"8","author":"Caso","year":"2008","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.psj.2024.103681_bib0005","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1637\/9147-111309-Review.1","article-title":"Clostridial dermatitis and cellulitis: An emerging disease of turkeys","volume":"54","author":"Clark","year":"2010","journal-title":"Avian Dis"},{"key":"10.1016\/j.psj.2024.103681_bib0006","doi-asserted-by":"crossref","first-page":"274","DOI":"10.2174\/156652408784533760","article-title":"Pathophysiological mechanisms of stress-induced intestina damage","volume":"8","author":"Gareau","year":"2008","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.psj.2024.103681_bib0008","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1177\/1040638717742435","article-title":"Gangrenous dermatitis in chickens and turkeys","volume":"30","author":"Gornatti-Churria","year":"2018","journal-title":"J. Vet. Diagn. Invest"},{"key":"10.1016\/j.psj.2024.103681_bib0009","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1128\/JCM.01975-09","article-title":"Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis","volume":"48","author":"Halm","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.psj.2024.103681_bib0010","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1637\/10522-030113-Reg.1","article-title":"Dexamethasone immunosuppression resulting in turkey clostridial dermatitis: A retrospective analysis of seven studies, 1998\u20132009","volume":"57","author":"Huff","year":"2013","journal-title":"Avian Dis"},{"key":"10.1016\/j.psj.2024.103681_bib0011","doi-asserted-by":"crossref","DOI":"10.1128\/mSystems.00095-16","article-title":"Impact of sample type and DNA isolation procedure on genomic inference of microbiome composition","volume":"1","author":"Knudsen","year":"2016","journal-title":"Msystems"},{"key":"10.1016\/j.psj.2024.103681_bib0014","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.mcp.2010.03.003","article-title":"Development and validation of a multiplex real-time PCR for detection of Clostridium chauvoei and Clostridium septicum","volume":"24","author":"Lange","year":"2010","journal-title":"Mol. Cell. Probes."},{"key":"10.1016\/j.psj.2024.103681_bib0015","doi-asserted-by":"crossref","first-page":"104","DOI":"10.3382\/japr\/pfv065","article-title":"Incidence of clostridial dermatitis (cellulitis) and factors for development of the disease in turkeys","volume":"25","author":"Lighty","year":"2016","journal-title":"J. Appl. Poult. Res"},{"key":"10.1016\/j.psj.2024.103681_bib0016","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/gt.2016.2","article-title":"Establishment of two quantitative nested qPCR assays targeting the human EPO transgene","volume":"23","author":"Neuberger","year":"2016","journal-title":"Gene Ther"},{"key":"10.1016\/j.psj.2024.103681_bib0017","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.mcp.2010.04.001","article-title":"Quantitative real-time PCR assay for Clostridium septicum in poultry gangrenous dermatitis associated samples","volume":"24","author":"Neumann","year":"2010","journal-title":"Mol. Cell. Probes."},{"key":"10.1016\/j.psj.2024.103681_bib0018","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.anaerobe.2009.01.005","article-title":"MLST analysis reveals a highly conserved core genome among poultry isolates of Clostridium septicum","volume":"15","author":"Neumann","year":"2009","journal-title":"Anaerobe"},{"key":"10.1016\/j.psj.2024.103681_bib0019","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1177\/104063870902100313","article-title":"Evidence for Clostridium septicum as a primary cause of cellulitis in commercial turkeys","volume":"21","author":"Tellez","year":"2009","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.psj.2024.103681_bib0020","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1637\/9009-080309-Reg.1","article-title":"Role of Clostridium perfringens and Clostridium septicum in causing turkey cellulitis","volume":"54","author":"Thachil","year":"2010","journal-title":"Avian Dis"},{"key":"10.1016\/j.psj.2024.103681_bib0021","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1186\/1475-2875-13-393","article-title":"A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots","volume":"13","author":"Tran","year":"2014","journal-title":"Malar. J."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124002621?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124002621?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T14:21:18Z","timestamp":1727274078000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579124002621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S0032579124002621"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2024.103681","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of real-time PCR assay for quantitative detection of Clostridium septicum","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2024.103681","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"103681"}}