{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:07:43Z","timestamp":1791198463755,"version":"4.1.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018530","name":"Major Science and Technology Projects in Anhui Province","doi-asserted-by":"publisher","award":["202003a06020012"],"award-info":[{"award-number":["202003a06020012"]}],"id":[{"id":"10.13039\/501100018530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.psj.2024.104452","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:35:11Z","timestamp":1730334911000},"page":"104452","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development and evaluation of three multienzyme isothermal rapid amplification assays for fowl adenovirus serotype 4"],"prefix":"10.1016","volume":"103","author":[{"given":"Yin","family":"Dai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyi","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fazhi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqin","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2781-0102","authenticated-orcid":false,"given":"Huiqin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieru","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1163-1305","authenticated-orcid":false,"given":"Dongdong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehuai","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocheng","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2024.104452_bib0001","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1136\/vr.128.25.591","article-title":"Studies on the aetiology of hydropericardium syndrome (Angara disease) in broilers","volume":"128","author":"Afzal","year":"1991","journal-title":"Vet. Rec."},{"key":"10.1016\/j.psj.2024.104452_bib0002","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s12917-024-04081-0","article-title":"Molecular characterisation of fowl adenovirus associated with hydropericardium hepatitis syndrome in broiler and layer breeders in Azerbaijan","volume":"20","author":"Bayraktar","year":"2024","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.psj.2024.104452_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.103144","article-title":"Multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for visible detection of chicken chaphamaparvovirus","volume":"102","author":"Cui","year":"2023","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0004","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jviromet.2018.09.005","article-title":"Development and evaluation of a droplet digital PCR assay for the detection of fowl adenovirus serotypes 4 and 10 in attenuated vaccines","volume":"265","author":"Dong","year":"2019","journal-title":"J. Virol. Methods."},{"key":"10.1016\/j.psj.2024.104452_bib0005","article-title":"Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: A comprehensive review","volume":"9","author":"El-Shall","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0006","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1080\/03079457.2022.2159326","article-title":"A multiplex fluorescence-based loop-mediated isothermal amplification assay for identifying chicken parvovirus, chicken infectious anaemia virus, and fowl aviadenovirus serotype 4","volume":"52","author":"Fan","year":"2023","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2024.104452_bib0007","first-page":"154","article-title":"Pathology and molecular epidemiology of fowl adenovirus serotype 4 outbreaks in broiler chicken in Abu Dhabi Emirate","volume":"23","author":"Ishag","year":"2022","journal-title":"UAE. Vet. Sci"},{"key":"10.1016\/j.psj.2024.104452_bib0008","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.1059934","article-title":"Development of a multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for bovine coronavirus detection","volume":"9","author":"Ji","year":"2023","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0009","doi-asserted-by":"crossref","first-page":"2630","DOI":"10.3382\/ps\/pex087","article-title":"Fowl adenovirus serotype 4: epidemiology, pathogenesis, diagnostic detection, and vaccine strategies","volume":"96","author":"Li","year":"2017","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0010","article-title":"Rapid detection of porcine circovirus type 4 via multienzyme isothermal rapid amplification","volume":"9","author":"Li","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0011","article-title":"Advances in vaccine development of the emerging novel genotype fowl adenovirus 4","volume":"13","author":"Liu","year":"2022","journal-title":"Front. Immunol"},{"key":"10.1016\/j.psj.2024.104452_bib0012","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1186\/s13568-021-01309-2","article-title":"Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4","volume":"11","author":"Liu","year":"2021","journal-title":"AMB Express"},{"key":"10.1016\/j.psj.2024.104452_bib0013","doi-asserted-by":"crossref","first-page":"803","DOI":"10.3382\/ps\/pex338","article-title":"Molecular epidemiology and phylogenetic analysis of fowl adenoviruses caused hydropericardium outbreak in China during 2015","volume":"97","author":"Niu","year":"2018","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0014","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3390\/pathogens13010057","article-title":"Development of multienzyme isothermal rapid amplification (MIRA) combined with lateral-flow dipstick (LFD) assay to detect species-specific tlh and pathogenic trh and tdh genes of Vibrio parahaemolyticus","volume":"13","author":"Park","year":"2024","journal-title":"Pathogens"},{"key":"10.1016\/j.psj.2024.104452_bib0015","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jviromet.2014.06.017","article-title":"Recombinant hexon antigen based single serum dilution ELISA for rapid serological profiling against fowl adenovirus-4 causing hydropericardium syndrome in chickens","volume":"207","author":"Rajasekhar","year":"2014","journal-title":"J. Virol. Methods."},{"key":"10.1016\/j.psj.2024.104452_bib0016","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s11262-019-01676-w","article-title":"Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens","volume":"55","author":"Ren","year":"2019","journal-title":"Virus Genes"},{"key":"10.1016\/j.psj.2024.104452_bib0017","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.vetimm.2007.09.017","article-title":"Fowl adenovirus (FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection","volume":"121","author":"Schonewille","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2024.104452_bib0018","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.7754\/Clin.Lab.2021.210239","article-title":"Duplex reverse transcription multienzyme isothermal rapid amplification assays for detecting SARS-CoV-2","volume":"67","author":"Shang","year":"2021","journal-title":"Clin. Lab."},{"key":"10.1016\/j.psj.2024.104452_bib0019","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1637\/0005-2086-65.3.385","article-title":"Molecular detection of a novel fowl adenovirus serotype-4 (FadV-4) from an outbreak of hepatitis hydropericardium syndrome in commercial broiler chickens in Egypt","volume":"65","author":"Sultan","year":"2021","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2024.104452_bib0020","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2021.763079","article-title":"Simple and Feasible detection of hepatitis B virus via combination of multienzyme isothermal rapid amplification and lateral flow dipstick strip","volume":"8","author":"Sun","year":"2021","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.psj.2024.104452_bib0021","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1080\/21691401.2023.2203198","article-title":"Simple and feasible detection of hepatitis a virus using reverse transcription multienzyme isothermal rapid amplification and lateral flow dipsticks without standard PCR laboratory","volume":"51","author":"Sun","year":"2023","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"10.1016\/j.psj.2024.104452_bib0022","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1637\/aviandiseases-D-21-00086","article-title":"The first occurrence of hepatitis-hydropericardium syndrome in iran and effective applied control measures in the affected commercial broiler flock","volume":"66","author":"Toroghi","year":"2022","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2024.104452_bib0023","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1080\/03079457.2016.1278428","article-title":"Development of a TaqMan-based real-time PCR assay for rapid and specific detection of fowl aviadenovirus serotype 4","volume":"46","author":"Wang","year":"2017","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2024.104452_bib0024","article-title":"Pathogenicity and epidemiological survey of fowl adenovirus in shandong province from 2021 to 2022","volume":"14","author":"Wang","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.psj.2024.104452_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virusres.2017.12.003","article-title":"Immune protection efficacy of FAdV-4 surface proteins fiber-1, fiber-2, hexon and penton base","volume":"245","author":"Wang","year":"2018","journal-title":"Virus Res."},{"key":"10.1016\/j.psj.2024.104452_bib0026","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1637\/aviandiseases-D-19-00127","article-title":"Epizootiology, clinical signs, and phylogenetic analysis of fowl adenovirus in chicken farms in Indonesia from 2018 to 2019","volume":"63","author":"Wibowo","year":"2019","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2024.104452_bib0027","first-page":"191","article-title":"The development of a multienzyme isothermal rapid amplification assay to visually detect duck hepatitis B Virus","volume":"11","author":"Xu","year":"2024","journal-title":"Vet. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0028","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104381","article-title":"Molecular characterization and pathogenicity of highly pathogenic fowl adenovirus serotype 4 isolated from laying flock with hydropericardium-hepatitis syndrome","volume":"147","author":"Yin","year":"2020","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.psj.2024.104452_bib0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.103729","article-title":"Pyrococcus furiosus argonaute combined with loop-mediated isothermal amplification for rapid, ultrasensitive, and visual detection of fowl adenovirus serotype 4","volume":"103","author":"Yu","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0031","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s13205-019-2006-z","article-title":"A simple and rapid diagnostic method to detect new goose astrovirus using reverse-transcription loop-mediated isothermal amplification","volume":"10","author":"Yu","year":"2020","journal-title":"3 Biotech"},{"key":"10.1016\/j.psj.2024.104452_bib0032","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1186\/s12917-019-2015-5","article-title":"LAMP real-time turbidity detection for fowl adenovirus","volume":"15","author":"Yuan","year":"2019","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.psj.2024.104452_bib0033","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jviromet.2019.04.026","article-title":"A loop-mediated isothermal amplification coupling with a lateral flow dipstick for rapid and specific detection of fowl adenovirus serotype-4","volume":"270","author":"Zhai","year":"2019","journal-title":"J. Virol. Methods."},{"key":"10.1016\/j.psj.2024.104452_bib0034","article-title":"Prevalence, genomic characteristics, and pathogenicity of fowl adenovirus 2 in Southern China","volume":"103","author":"Zhang","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2024.104452_bib0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109285","article-title":"An inactivated vaccine based on artificial non-pathogenic fowl adenovirus 4 protects chickens against hepatitis-hydropericardium syndrome","volume":"264","author":"Zhang","year":"2022","journal-title":"Vet. Microbiol."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124010307?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579124010307?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T20:44:32Z","timestamp":1776458672000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579124010307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["S0032579124010307"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2024.104452","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and evaluation of three multienzyme isothermal rapid amplification assays for fowl adenovirus serotype 4","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2024.104452","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"104452"}}