{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T17:16:07Z","timestamp":1767892567281,"version":"3.49.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2017-67015-26793"],"award-info":[{"award-number":["2017-67015-26793"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.psj.2025.105112","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T01:42:37Z","timestamp":1743990157000},"page":"105112","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Construction of a Bacillus subtilis-based expression system for Eimeria acervulina profilin"],"prefix":"10.1016","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2520-9746","authenticated-orcid":false,"given":"Alfredo","family":"Panebra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngsub","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7755-6216","authenticated-orcid":false,"given":"Hyun S.","family":"Lillehoj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.psj.2025.105112_bib0001","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s43170-021-00056-5","article-title":"Controlling the causative agents of coccidiosis in domestic chickens; an eye on the past and considerations for the future","volume":"2","author":"Attree","year":"2021","journal-title":"CABI Agric. Biosci."},{"key":"10.1016\/j.psj.2025.105112_bib0002","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/978-1-61779-182-6_11","article-title":"Synchronization of Bacillus subtilis cells by spore germination and outgrowth","volume":"761","author":"Banfalvi","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.psj.2025.105112_bib0003","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.meegid.2017.10.009","article-title":"Recombinant anticoccidial vaccines - a cup half full?","volume":"55","author":"Blake","year":"2017","journal-title":"Infect. Genet. Evol."},{"issue":"1","key":"10.1016\/j.psj.2025.105112_bib0004","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pt.2013.10.003","article-title":"Securing poultry production from the ever-present Eimeria challenge","volume":"30","author":"Blake","year":"2014","journal-title":"Trends Parasitol."},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0005","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.3390\/life13061295","volume":"13","author":"Britez","year":"2023","journal-title":"Life"},{"key":"10.1016\/j.psj.2025.105112_bib0006","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1111\/jam.14192","article-title":"Engineering Bacillus for efficient production of heterologous protein: current progress, challenge and prospect","volume":"126","author":"Cai","year":"2019","journal-title":"J. App. Microbiol."},{"issue":"16","key":"10.1016\/j.psj.2025.105112_bib0007","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1128\/AEM.00580-08","article-title":"Bacillus subtilis spores germinate in the chicken gastrointestinal tract","volume":"74","author":"Cartman","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"issue":"Pt 2","key":"10.1016\/j.psj.2025.105112_bib0008","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1099\/mic.0.022939-0","article-title":"Phagocytosis, germination and killing of Bacillus subtilis spores presenting heterologous antigens in human macrophages","volume":"155","author":"Ceragioli","year":"2009","journal-title":"Microbiology"},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0009","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s11274-024-03997-x","article-title":"Bacillus subtilis: current and future modification strategies as a protein secreting factory","volume":"40","author":"Chen","year":"2024","journal-title":"World J. Microbiol. Biotechnol."},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0010","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1016\/j.psj.2020.03.014","article-title":"Immune response and protective efficacy of recombinant Enterococcus faecalis displaying dendritic cell-targeting peptide fused with Eimeria tenella 3-1E protein","volume":"99","author":"Chen","year":"2020","journal-title":"Poult. Sci."},{"issue":"9","key":"10.1016\/j.psj.2025.105112_bib0011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0025233","article-title":"Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages","volume":"6","author":"Chow","year":"2011","journal-title":"PLoS One"},{"issue":"10","key":"10.1016\/j.psj.2025.105112_bib0012","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11274-018-2531-7","article-title":"Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond","volume":"34","author":"Cui","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"issue":"12","key":"10.1016\/j.psj.2025.105112_bib0013","doi-asserted-by":"crossref","first-page":"6939","DOI":"10.1128\/IAI.72.12.6939-6944.2004","article-title":"Protective immunity against Eimeria acervulina following in ovo immunization with a recombinant subunit vaccine and cytokine genes","volume":"72","author":"Ding","year":"2004","journal-title":"Infect. Immun."},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0014","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1089\/omi.2010.0069","article-title":"Transcriptional regulation in yeast during diauxic shift and stationary phase","volume":"14","author":"Galdieri","year":"2010","journal-title":"OMICS"},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0015","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1128\/JB.01668-08","article-title":"Isolation and characterization of super dormant spores of Bacillus species","volume":"191","author":"Ghosh","year":"2009","journal-title":"J. Bacteriol."},{"issue":"9","key":"10.1016\/j.psj.2025.105112_bib0016","doi-asserted-by":"crossref","first-page":"lxad213","DOI":"10.1093\/jambio\/lxad213","article-title":"Probiotics as an alternative to antibiotics in modulating the intestinal microbiota and performance of broiler chickens","volume":"134","author":"Grozina","year":"2023","journal-title":"J. Appl. Microbiol."},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0017","first-page":"e105","volume":"33","author":"Goo","year":"2023","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.psj.2025.105112_bib0018","doi-asserted-by":"crossref","unstructured":"Guo M., Li M., Zhang C., Zhang X., and Wu Y.. 2020. Dietary administration of the Bacillus subtilis enhances immune responses and disease resistance in chickens. 11:1-11. 10.3389\/fmicb.2020.01768.","DOI":"10.3389\/fmicb.2020.01768"},{"issue":"18","key":"10.1016\/j.psj.2025.105112_bib0019","doi-asserted-by":"crossref","DOI":"10.1128\/mra.01380-18","article-title":"Complete genome sequence of Bacillus subtilis strain WB800N, an extracellular protease-deficient derivative of strain 168","volume":"7","author":"Jeong","year":"2018","journal-title":"Microbiol. Resour. Announc."},{"key":"10.1016\/j.psj.2025.105112_bib0020","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1007\/s10295-014-1506-4","article-title":"Molecular engineering of secretory machinery components for high\u2011level secretion of proteins in Bacillus species","volume":"41","author":"Kang","year":"2014","journal-title":"J. Ind. Microbiol. Biotechnol."},{"issue":"9","key":"10.1016\/j.psj.2025.105112_bib0021","doi-asserted-by":"crossref","first-page":"3664","DOI":"10.1128\/iai.60.9.3664-3672.1992","article-title":"Macrophage response to bacteria: induction of marked secretory and cellular activities by lipoteichoic acids","volume":"60","author":"Keller","year":"1992","journal-title":"Infect. Immunity"},{"key":"10.1016\/j.psj.2025.105112_bib0022","first-page":"2373","article-title":"Shifts from glucose to certain secondary carbon-sources result in activation of the extracytoplasmic function sigma factor \u03c3E in Salmonella enterica serovar Typhimurium","volume":"151","author":"Kenyon","year":"2005","journal-title":"Microbiology (N. Y)"},{"key":"10.1016\/j.psj.2025.105112_bib0023","unstructured":"Kolodkin-Gal I. 2015. Colony PCR for Bacillus subtilis. iGEM Technion_Israel 2015. http:\/\/2015.igem.org\/Team:Technion_Israel."},{"issue":"3","key":"10.1016\/j.psj.2025.105112_bib0024","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0378-1135(03)00077-4","article-title":"Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype enteritidis and Clostridium perfringens in young chickens","volume":"94","author":"La Ragione","year":"2003","journal-title":"Vet. Microbiol."},{"issue":"2","key":"10.1016\/j.psj.2025.105112_bib0025","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3390\/microorganisms11020269","article-title":"Probiotic Bacillus strains enhance T cell responses in chicken","volume":"11","author":"Larsberg","year":"2023","journal-title":"Microorganisms"},{"issue":"7","key":"10.1016\/j.psj.2025.105112_bib0026","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.3382\/ps.2013-03809","article-title":"Evaluation of germination, distribution, and persistence of Bacillus subtilis spores through the gastrointestinal tract of chickens","volume":"93","author":"Latorre","year":"2014","journal-title":"Poult. Sci."},{"issue":"6","key":"10.1016\/j.psj.2025.105112_bib0027","doi-asserted-by":"crossref","first-page":"e105","DOI":"10.1016\/j.cimid.2010.06.001","article-title":"Effect of Bacillus-based direct-fed microbials on Eimeria maxima infection in broiler chickens","volume":"33","author":"Lee","year":"2010","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"issue":"8","key":"10.1016\/j.psj.2025.105112_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.102790","article-title":"Development of a new immunodiagnostic tool for poultry coccidiosis using an antigen-capture sandwich assay based on monoclonal antibodies detecting an immunodominant antigen of Eimeria","volume":"102","author":"Lee","year":"2023","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105112_bib0031","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2307\/1592553","article-title":"A recombinant Eimeria protein inducing interferon-\u03b3 production. Comparison of different gene expression systems and immunization strategies for vaccination against coccidiosis","volume":"44","author":"Lillehoj","year":"2000","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2025.105112_bib0032","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1017\/S1466252300000050","article-title":"Vaccine for avian enteropathogens eimeria, cryptosporidium and Salmonella","volume":"1","author":"Lillehoj","year":"2000","journal-title":"Anim. Health Res. Rev."},{"issue":"9","key":"10.1016\/j.psj.2025.105112_bib0034","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.1007\/s00436-015-4539-3","article-title":"Protective immunity against eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing eimeria tenella 3-1E protein","volume":"114","author":"Lin","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.psj.2025.105112_bib0035","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/2549541","article-title":"Supplemental Bacillus subtilis PB6 improves growth performance and gut health in broilers challenged with Clostridium perfringens","volume":"2021","author":"Liu","year":"2021","journal-title":"J. Immunol. Res."},{"issue":"1","key":"10.1016\/j.psj.2025.105112_bib0036","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1186\/s12917-018-1665-z","article-title":"Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima","volume":"14","author":"Liu","year":"2018","journal-title":"BMC Vet. Res."},{"issue":"4","key":"10.1016\/j.psj.2025.105112_bib0037","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2000","journal-title":"Methods"},{"key":"10.1016\/j.psj.2025.105112_bib0038","doi-asserted-by":"crossref","first-page":"4161","DOI":"10.1007\/s00436-013-3607-9","article-title":"Protective effects of oral immunization with live lactococcus lactis expressing Eimeria tenella 3-1E protein","volume":"112","author":"Ma","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.psj.2025.105112_bib0039","series-title":"Molecular Biological Methods for Bacillus","first-page":"391","article-title":"Sporulation, germination and outgrowth","author":"Nicholson","year":"1990"},{"issue":"9","key":"10.1016\/j.psj.2025.105112_bib0040","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1128\/JB.182.9.2513-2519.2000","article-title":"Role of ger proteins in nutrient and non-nutrient triggering of spore germination in Bacillus subtilis","volume":"182","author":"Paidhungat","year":"2000","journal-title":"J. Bacteriol."},{"issue":"10","key":"10.1016\/j.psj.2025.105112_bib0041","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.102945","article-title":"Lactobacillus plantarum surface.displayed eimeria tenella profilin antigens with FliC flagellin elicit protection against coccidiosis in chickens","volume":"102","author":"Pan","year":"2023","journal-title":"Poult. Sci."},{"issue":"3","key":"10.1016\/j.psj.2025.105112_bib0042","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2020.12.037","article-title":"Research note: characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7","volume":"100","author":"Panebra","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105112_bib0043","first-page":"115","article-title":"Mucosal vaccine delivery by non.recombinant spores of Bacillus subtilis","volume":"13","author":"Ricca","year":"2014","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.psj.2025.105112_bib0044","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241310880","article-title":"The bacterial spore as a mucosal vaccine delivery system","volume":"24","author":"Saggese","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.psj.2025.105112_bib0045","first-page":"285.330","article-title":"The biology of avian eimeria with emphasis on their control by vaccination","volume":"60","author":"Shirley","year":"2005","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.psj.2025.105112_bib0046","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.3389\/fimmu.2017.01982","article-title":"A novel vaccine delivery model of the apicomplexan Eimeria tenella expressing Eimeria maxima antigen protects chickens against infection of the two parasites","volume":"8","author":"Tang","year":"2018","journal-title":"Front. Immunol."},{"issue":"4","key":"10.1016\/j.psj.2025.105112_bib0047","doi-asserted-by":"crossref","first-page":"317.53","DOI":"10.1080\/03079450220148988","article-title":"Anticoccidial vaccines for broiler chickens: pathways to success","volume":"31","author":"Williams","year":"2002","journal-title":"Avian Pathol."},{"issue":"4","key":"10.1016\/j.psj.2025.105112_bib0048","doi-asserted-by":"crossref","first-page":"775.802","DOI":"10.1637\/0005-2086(2002)046[0775:FYOAVF]2.0.CO;2","article-title":"Fifty years of anticoccidial vaccines for poultry (1952.2002)","volume":"46","author":"Williams","year":"2002","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2025.105112_bib0049","first-page":"1335.1345","article-title":"Dissection of the expression signals of the spoUA gene of Bacillus subtilis: glucose represses sporulation.specific expression","volume":"135","author":"Yamashita","year":"1989","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/j.psj.2025.105112_bib0050","doi-asserted-by":"crossref","first-page":"303.324","DOI":"10.1016\/S0145-305X(99)00080-4","article-title":"Intestinal immune responses to coccidiosis","volume":"24","author":"Yun","year":"2000","journal-title":"Dev. Comp. Immunol."},{"issue":"3","key":"10.1016\/j.psj.2025.105112_bib0052","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2020.12.032","article-title":"Dietary supplementation with Bacillus subtilis promotes growth performance of broilers by altering the dominant microbial community","volume":"100","author":"Zhang","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105112_bib0053","doi-asserted-by":"crossref","first-page":"4238","DOI":"10.1038\/s41467-024-48591-9","article-title":"Shaping of microbial phenotypes by trade.offs","volume":"15","author":"Zhu","year":"2024","journal-title":"Nat. Commun."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125003517?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125003517?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T02:27:09Z","timestamp":1749004029000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579125003517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S0032579125003517"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2025.105112","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction of a Bacillus subtilis-based expression system for Eimeria acervulina profilin","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2025.105112","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"105112"}}