{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:07:22Z","timestamp":1785773242136,"version":"3.56.0"},"reference-count":42,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","award":["JSSCTD202224"],"award-info":[{"award-number":["JSSCTD202224"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273016"],"award-info":[{"award-number":["32273016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012154","name":"Graduate Research and Innovation Projects of Jiangsu Province","doi-asserted-by":"publisher","award":["SJCX24-2311"],"award-info":[{"award-number":["SJCX24-2311"]}],"id":[{"id":"10.13039\/501100012154","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.psj.2025.105524","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:59:51Z","timestamp":1752573591000},"page":"105524","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of an ELISPOT assay for numerating IFN-\u03b3-secreting T cells in chicken using novel monoclonal antibodies"],"prefix":"10.1016","volume":"104","author":[{"given":"Zhou","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huining","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjie","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4407-1911","authenticated-orcid":false,"given":"Shaobin","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2025.105524_bib0001","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.vetimm.2017.10.001","article-title":"Quantification and phenotypic characterisation of peripheral IFN-\u03b3 producing leucocytes in chickens vaccinated against Newcastle disease","volume":"193-194","author":"Andersen","year":"2017","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2025.105524_bib0002","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1016\/j.dci.2008.05.007","article-title":"ELISPOT and intracellular cytokine staining: novel assays for quantifying T cell responses in the chicken","volume":"32","author":"Ariaans","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0003","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-1-0716-1771-7_5","article-title":"Detection of cytokine-secreting cells by enzyme-linked immunospot (ELISpot)","volume":"2386","author":"Axelsson","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.psj.2025.105524_bib0004","article-title":"Differential virus-specific IFN-gamma producing T cell responses to Marek's disease virus in chickens with B19 and B21 MHC haplotypes","volume":"12","author":"Boodhoo","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0005","article-title":"Marek's disease virus-specific T cells proliferate, express antiviral cytokines but have impaired degranulation response","volume":"13","author":"Boodhoo","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0006","first-page":"134","article-title":"Development of a double-monoclonal antibody sandwich ELISA: tool for chicken interferon-\u03b3 detection ex vivo","volume":"80","author":"Dai","year":"2016","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.psj.2025.105524_bib0007","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.psj.2020.09.080","article-title":"Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen-free chicken","volume":"100","author":"Dai","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105524_bib0008","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1158\/2326-6066.CIR-18-0129","article-title":"The mutation-associated neoantigen functional expansion of specific T cells (MANAFEST) assay: a sensitive platform for monitoring antitumor immunity","volume":"6","author":"Danilova","year":"2018","journal-title":"Cancer Immunol. Res."},{"key":"10.1016\/j.psj.2025.105524_bib0009","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1089\/jir.1995.15.939","article-title":"Cloning and expression of the chicken interferon-gamma gene","volume":"15","author":"Digby","year":"1995","journal-title":"J. Interferon Cytokine Res."},{"key":"10.1016\/j.psj.2025.105524_bib0010","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1089\/jir.2018.0034","article-title":"Recombinant duck interferon gamma inhibits H5N1 influenza virus replication in vitro and in vivo","volume":"38","author":"Gao","year":"2018","journal-title":"J. Interferon Cytokine Res."},{"key":"10.1016\/j.psj.2025.105524_bib0011","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/jgm.1432","article-title":"Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination","volume":"12","author":"Guan","year":"2010","journal-title":"J. Gene Med."},{"key":"10.1016\/j.psj.2025.105524_bib0012","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01322-23","article-title":"Co-administration of chicken IL-2 alleviates clinical signs and replication of the ILTV chicken embryo origin vaccine by pre-activating natural killer cells and cytotoxic T lymphocytes","volume":"97","author":"Hao","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.psj.2025.105524_bib0013","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.645426","article-title":"An anti-tumor vaccine against Marek's disease virus induces differential activation and memory response of gammadelta T cells and CD8 T cells in chickens","volume":"12","author":"Hao","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0014","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.794514","article-title":"The evaluation of cellular immunity to avian viral diseases: methods, applications, and challenges","volume":"12","author":"Hao","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.psj.2025.105524_bib0015","first-page":"1","article-title":"Effects of interferon-\u03b3 knockdown on vaccine-induced immunity against Marek's disease in chickens","volume":"79","author":"Haq","year":"2015","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.psj.2025.105524_bib0016","doi-asserted-by":"crossref","DOI":"10.1016\/j.jvacx.2021.100128","article-title":"B cells do not play a role in vaccine-mediated immunity against Marek's disease","volume":"10","author":"Heidari","year":"2022","journal-title":"Vaccine X"},{"key":"10.1016\/j.psj.2025.105524_bib0017","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3390\/v15030648","article-title":"Role of T cells in vaccine-mediated immunity against Marek's disease","volume":"15","author":"Heidari","year":"2023","journal-title":"Viruses"},{"key":"10.1016\/j.psj.2025.105524_bib0018","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0304-4017(00)00275-2","article-title":"Inhibition of Eimeria tenella replication after recombinant IFN-gamma activation in chicken macrophages, fibroblasts and epithelial cells","volume":"92","author":"H\u00e9riveau","year":"2000","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.psj.2025.105524_bib0019","doi-asserted-by":"crossref","first-page":"6585","DOI":"10.1016\/j.vaccine.2017.10.010","article-title":"Newcastle disease virus (NDV) recombinant expressing the hemagglutinin of H7N9 avian influenza virus protects chickens against NDV and highly pathogenic avian influenza A (H7N9) virus challenges","volume":"35","author":"Hu","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.psj.2025.105524_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107395","article-title":"Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 avian influenza virus (AIV)","volume":"300","author":"Jia","year":"2024","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2025.105524_bib0021","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.3390\/ani12213040","article-title":"Optimization of capture ELISAs for chicken cytokines using commercially available antibodies","volume":"12","author":"Krzysica","year":"2022","journal-title":"Animals"},{"key":"10.1016\/j.psj.2025.105524_bib0022","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s13567-019-0726-z","article-title":"Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken","volume":"50","author":"Lagler","year":"2019","journal-title":"Vet. Res."},{"key":"10.1016\/j.psj.2025.105524_bib0023","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103949","article-title":"Comparative investigation of IFN-\u03b3-producing T cells in chickens and turkeys following vaccination and infection with the extracellular parasite Histomonas meleagridis","volume":"116","author":"Lagler","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0024","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0165-2427(00)00165-3","article-title":"Production of antibodies against chicken interferon-gamma: demonstration of neutralizing activity and development of a quantitative ELISA","volume":"74","author":"Lambrecht","year":"2000","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2025.105524_bib0025","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0165-2427(99)00080-X","article-title":"Comparison of biological activities of natural and recombinant chicken interferon-gamma","volume":"70","author":"Lambrecht","year":"1999","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2025.105524_bib0026","doi-asserted-by":"crossref","first-page":"e33","DOI":"10.1038\/emi.2016.27","article-title":"DNA immunization as a technology platform for monoclonal antibody induction","volume":"5","author":"Liu","year":"2016","journal-title":"Emerg. Microbes Infect."},{"key":"10.1016\/j.psj.2025.105524_bib0027","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1089\/hum.2018.077","article-title":"Using DNA immunization to elicit monoclonal antibodies in mice, rabbits, and humans","volume":"29","author":"Liu","year":"2018","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.psj.2025.105524_bib0028","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1089\/jir.1995.15.933","article-title":"Production of interferon-gamma by chicken T cells","volume":"15","author":"Lowenthal","year":"1995","journal-title":"J. Interferon Cytokine Res."},{"key":"10.1016\/j.psj.2025.105524_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetimm.2024.110802","article-title":"Glycerides of lauric acid supplementation in the chicken diet enhances the humoral and cellular immune response to infectious bronchitis virus","volume":"274","author":"Mellouk","year":"2024","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2025.105524_bib0030","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1089\/hyb.2008.0098","article-title":"Three different immunogen preparation strategies for production of CD4 monoclonal antibodies","volume":"28","author":"Pata","year":"2009","journal-title":"Hybridoma"},{"key":"10.1016\/j.psj.2025.105524_bib0031","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00667-21","article-title":"Identification of novel T-cell epitopes on infectious Bronchitis virus N protein and development of a multi-epitope vaccine","volume":"95","author":"Qin","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.psj.2025.105524_bib0032","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0031953","article-title":"Identification of novel avian influenza virus derived CD8+ T-cell epitopes","volume":"7","author":"Reemers","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.psj.2025.105524_bib0033","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0022-1759(97)00184-1","article-title":"Evaluation of the interferon-gamma ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood","volume":"210","author":"Schmittel","year":"1997","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.psj.2025.105524_bib0034","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/978-1-4939-2815-6_16","article-title":"The ex vivo IFN-\u03b3 enzyme-linked immunospot (ELISpot) assay","volume":"1325","author":"Sedegah","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.psj.2025.105524_bib0035","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1637\/11965-090118-Reg.1","article-title":"Single immunization with Newcastle disease virus-vectored H7N9 vaccine confers a complete protection against challenge with highly pathogenic Avian influenza H7N9 virus","volume":"63","author":"Shi","year":"2019","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2025.105524_bib0036","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0165-2427(97)00034-2","article-title":"Expression and functional characterization of recombinant chicken interferon-gamma","volume":"58","author":"Song","year":"1997","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.psj.2025.105524_bib0037","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.micpath.2013.05.009","article-title":"Expression of interferon gamma by a highly virulent strain of Newcastle disease virus decreases its pathogenicity in chickens","volume":"61-62","author":"Susta","year":"2013","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.psj.2025.105524_bib0038","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1100594","article-title":"Persistent memory despite rapid contraction of circulating T Cell responses to SARS-CoV-2 mRNA vaccination","volume":"14","author":"Taus","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.psj.2025.105524_bib0039","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.3390\/v14061343","article-title":"Development of a monoclonal antibody to pig CD69 reveals early activation of T cells in pig after PRRSV and ASFV infection","volume":"14","author":"Tian","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.psj.2025.105524_bib0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01652176.2023.2228360","article-title":"The immune system of chicken and its response to H9N2 avian influenza virus","volume":"43","author":"Yang","year":"2023","journal-title":"Vet. Q."},{"key":"10.1016\/j.psj.2025.105524_bib0041","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/abbs\/gmz158","article-title":"IFN-\u03b3 establishes interferon-stimulated gene-mediated antiviral state against Newcastle disease virus in chicken fibroblasts","volume":"52","author":"Yang","year":"2020","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"10.1016\/j.psj.2025.105524_bib0042","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0165-2427(00)00146-X","article-title":"Chicken IFN-gamma monoclonal antibodies and their application in enzyme-linked immunosorbent assay","volume":"73","author":"Yun","year":"2000","journal-title":"Vet. Immunol. Immunopathol."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125007679?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125007679?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:19:52Z","timestamp":1777461592000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579125007679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["S0032579125007679"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2025.105524","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of an ELISPOT assay for numerating IFN-\u03b3-secreting T cells in chicken using novel monoclonal antibodies","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2025.105524","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"105524"}}