{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:41:23Z","timestamp":1787503283771,"version":"build-2736575974"},"reference-count":45,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100021025","name":"Ministry of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100021025","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.psj.2025.105840","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T15:42:46Z","timestamp":1757605366000},"page":"105840","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Beyond concentration: the role of inseminated glycerol volume on fertility outcomes in chickens"],"prefix":"10.1016","volume":"104","author":[{"given":"Ming-Yang","family":"Tsai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsi-Wen","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Chun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chiu","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Yi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1438-8950","authenticated-orcid":false,"given":"Hsiu-Lien Herbie","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2025.105840_bib0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-024-51481-9","article-title":"Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination","volume":"15","author":"Abdul Halim","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.psj.2025.105840_bib0002","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/rda.12464","article-title":"Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen\/thawed chicken sperm variables","volume":"50","author":"Abouelezz","year":"2015","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.psj.2025.105840_bib0003","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.anireprosci.2017.07.021","article-title":"Fertility disturbances of dimethylacetamide and glycerol in rooster sperm diluents: discrimination among effects produced pre and post freezing-thawing process","volume":"184","author":"Abouelezz","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0004","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.3390\/antiox11061054","article-title":"Exogenous melatonin ameliorates the negative effect of osmotic stress in human and bovine ovarian stromal cells","volume":"11","author":"Asadi","year":"2022","journal-title":"Antioxidants"},{"key":"10.1016\/j.psj.2025.105840_bib0005","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1089\/rej.2014.1656","article-title":"Cryoprotectant toxicity: facts, issues, and questions","volume":"18","author":"Best","year":"2015","journal-title":"Rejuvenation Res."},{"key":"10.1016\/j.psj.2025.105840_bib0006","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2021.106845","article-title":"Assessing different liquid-storage temperatures for rooster spermatozoa","volume":"233","author":"Blank","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0007","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1017\/S175173110700081X","article-title":"Specific features of in vivo and in vitro sperm storage in birds","volume":"1","author":"Blesbois","year":"2007","journal-title":"Animal"},{"key":"10.1016\/j.psj.2025.105840_bib0008","unstructured":"Boes, J., P. Boettcher, M. Honkatukia, and eds. 2023. Innovations in Cryoconservation of Animal Genetic Resources \u2013 Practical Guide. FAO Animal Production and Health Guidelines, No. 33, Rome."},{"key":"10.1016\/j.psj.2025.105840_bib0009","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3382\/ps.0160019","article-title":"The collection of spermatozoa from the domestic fowl and turkey","volume":"16","author":"Burrows","year":"1937","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0010","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1561144","article-title":"Characterization of the vitrification parameters for oviduct aggregates in Camelus dromedarius","volume":"12","author":"El-Sokary","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0011","first-page":"25","article-title":"A review article of artificial insemination in poultry. World\u2019s","volume":"6","author":"Getachew","year":"2016","journal-title":"Vet. J."},{"key":"10.1016\/j.psj.2025.105840_bib0012","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1093\/humupd\/dml035","article-title":"Function of aquaporins in female and male reproductive systems","volume":"12","author":"Huang","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.psj.2025.105840_bib0013","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1071\/RD22219","article-title":"Sperm cryopreservation: current status and future developments","volume":"35","author":"Hungerford","year":"2022","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.psj.2025.105840_bib0014","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0119388","article-title":"Expression pattern of inflammatory response genes and their regulatory micrornas in bovine oviductal cells in response to lipopolysaccharide: implication for early embryonic development","volume":"10","author":"Ibrahim","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.psj.2025.105840_bib0015","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1051\/rnd:19810806","article-title":"An attempt to improve the fertility of stored fowl semen with certain additives in a basic diluent","volume":"21","author":"Lake","year":"1981","journal-title":"Reprod. Nutr. Dev."},{"key":"10.1016\/j.psj.2025.105840_bib0016","doi-asserted-by":"crossref","first-page":"333","DOI":"10.3382\/ps.0670333","article-title":"Influence of storage container for frozen-thawed chicken semen and frequency of insemination on fertility and its duration","volume":"67","author":"Latorre","year":"1988","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0017","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1080\/00439339.2022.1998816","article-title":"Chicken semen cryopreservation: importance of cryoprotectants","volume":"78","author":"Lin","year":"2021","journal-title":"Worlds Poult. Sci. J."},{"key":"10.1016\/j.psj.2025.105840_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.cryobiol.2023.104567","article-title":"A simple and fast alternative method to remove glycerol from chicken semen after cryopreservation","volume":"112","author":"Lin","year":"2023","journal-title":"Cryobiology"},{"key":"10.1016\/j.psj.2025.105840_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.103448","article-title":"Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol","volume":"103","author":"Lin","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2023.107330","article-title":"Is glycerol a good cryoprotectant for sperm cells? New exploration of its toxicity using avian model","volume":"258","author":"Lin","year":"2023","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0021","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1093\/ps\/83.9.1594","article-title":"An effective method for improving the fertility of glycerol-exposed poultry semen","volume":"83","author":"Long","year":"2004","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0022","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1016\/j.theriogenology.2011.10.033","article-title":"Toxicity of glycerol for the stallion spermatozoa: effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential","volume":"77","author":"Mac\u00edas Garc\u00eda","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.psj.2025.105840_bib0023","doi-asserted-by":"crossref","first-page":"6212","DOI":"10.1016\/j.psj.2020.08.041","article-title":"Poloxamer 188 exerts a cryoprotective effect on rooster sperm and allows decreasing glycerol concentration in the freezing extender","volume":"99","author":"Mehdipour","year":"2020","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0024","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.cryobiol.2006.01.003","article-title":"The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation","volume":"52","author":"Morris","year":"2006","journal-title":"Cryobiology"},{"key":"10.1016\/j.psj.2025.105840_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104003","article-title":"Oxidative stress in poultry production","volume":"103","author":"Oke","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0026","doi-asserted-by":"crossref","first-page":"4716","DOI":"10.3390\/cimb45060300","article-title":"Sperm cryopreservation today: approaches, efficiency, and pitfalls","volume":"45","author":"Ozimic","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.psj.2025.105840_bib0027","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/167949b0","article-title":"Functional survival of fowl spermatozoa after freezing at \u221279 \u00b0C","volume":"167","author":"Polge","year":"1951","journal-title":"Nature"},{"key":"10.1016\/j.psj.2025.105840_bib0028","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.3390\/cells12152003","article-title":"Aquaporin-7-mediated glycerol permeability is linked to human sperm motility in asthenozoospermia and during sperm capacitation","volume":"12","author":"Ribeiro","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.psj.2025.105840_bib0029","doi-asserted-by":"crossref","first-page":"15","DOI":"10.7831\/ras.8.0_15","article-title":"Motility, viability and fertilizing ability of avian sperm stored under in vitro conditions","volume":"8","author":"Sarkar","year":"2020","journal-title":"Rev. Agric. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0030","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/0093-691X(95)00119-S","article-title":"Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa","volume":"43","author":"Seigneurin","year":"1995","journal-title":"Theriogenology"},{"key":"10.1016\/j.psj.2025.105840_bib0031","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1369\/jhc.2008.952499","article-title":"Immunolocalization of aquaporin 1, 5, and 9 in the female pig reproductive system","volume":"57","author":"Skowronski","year":"2009","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.psj.2025.105840_bib0032","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40104-022-00768-2","article-title":"Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges","volume":"13","author":"Sun","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.psj.2025.105840_bib0033","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-023-48550-2","article-title":"Arresting calcium-regulated sperm metabolic dynamics enables prolonged fertility in poultry liquid semen storage","volume":"13","author":"Sushadi","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.psj.2025.105840_bib0034","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2141\/jpsa.0120083","article-title":"Conservation of avian genetic resources","volume":"50","author":"Tajima","year":"2013","journal-title":"J. Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0035","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.3390\/ijms21082777","article-title":"Changes in aquaporin 1, 5 and 9 gene expression in the porcine oviduct according to estrous cycle and early pregnancy","volume":"21","author":"Tanski","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0036","doi-asserted-by":"crossref","first-page":"447","DOI":"10.3382\/ps\/pey360","article-title":"Chicken semen cryopreservation and use for the restoration of rare genetic resources","volume":"98","author":"Th\u00e9lie","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0037","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1093\/ps\/78.4.586","article-title":"Comparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa","volume":"78","author":"Tselutin","year":"1999","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0038","article-title":"The physiological and evolutionary ecology of sperm thermal performance","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Physiol."},{"key":"10.1016\/j.psj.2025.105840_bib0039","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.3390\/ani10081266","article-title":"Impact of heat stress on poultry health and performances, and potential mitigation strategies","volume":"10","author":"Wasti","year":"2020","journal-title":"Animals"},{"key":"10.1016\/j.psj.2025.105840_bib0040","doi-asserted-by":"crossref","first-page":"924","DOI":"10.3382\/ps.0560924","article-title":"Duration of the antifertility effect on glycerol in the chicken vagina","volume":"56","author":"Westfall","year":"1977","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0041","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/978-1-0716-0783-1_16","article-title":"Cryopreservation of avian semen","volume":"2180","author":"Woelders","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.psj.2025.105840_bib0042","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.3382\/ps.0631475","article-title":"Effect of selection for fertility of frozen-thawed semen in chickens on the fertility of fresh and stored semen","volume":"63","author":"Yousif","year":"1984","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.105840_bib0043","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/S0015-0282(16)56532-7","article-title":"Human oviductal fluid prolongs sperm survival","volume":"61","author":"Zhu","year":"1994","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.psj.2025.105840_bib0044","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3390\/agriculture13020445","article-title":"Chicken sperm cryopreservation: review of techniques, freezing damage, and freezability mechanisms","volume":"13","author":"Zong","year":"2023","journal-title":"Agriculture"},{"key":"10.1016\/j.psj.2025.105840_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.101840","article-title":"Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen","volume":"101","author":"Zong","year":"2022","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125010818?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125010818?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T03:19:56Z","timestamp":1773112796000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579125010818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":45,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S0032579125010818"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2025.105840","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Beyond concentration: the role of inseminated glycerol volume on fertility outcomes in chickens","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2025.105840","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"105840"}}