{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:34:17Z","timestamp":1777905257272,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015335","name":"Modern Agricultural Technology Industry System of Shandong province","doi-asserted-by":"publisher","award":["2023HBSTX4 - 04"],"award-info":[{"award-number":["2023HBSTX4 - 04"]}],"id":[{"id":"10.13039\/501100015335","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.psj.2025.106126","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T09:09:30Z","timestamp":1763629770000},"page":"106126","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid nucleic acid detection of Mycoplasma synoviae using dual-mode RAA\u2013CRISPR\/Cas12a system"],"prefix":"10.1016","volume":"105","author":[{"given":"Qiao","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunqing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongrong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bokai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinguo","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingping","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2025.106126_bib0001","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.102889","article-title":"Multidrug resistant and multivirulent avian bacterial pathogens: tackling experimental leg disorders using phytobiotics and antibiotics alone or in combination","volume":"102","author":"Awad","year":"2023","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0002","doi-asserted-by":"crossref","first-page":"3134","DOI":"10.3382\/ps.2013-03332","article-title":"Effect of Mycoplasma synoviae and lentogenic Newcastle disease virus coinfection on cytokine and chemokine gene expression in chicken embryos","volume":"92","author":"Bolha","year":"2013","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0003","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1111\/tbed.14302","article-title":"Pooled molecular occurrence of Mycoplasma gallisepticum and Mycoplasma synoviae in poultry: a systematic review and meta-analysis","volume":"69","author":"Chaidez-Ibarra","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.psj.2025.106126_bib0004","article-title":"From lab to field: innovative RPA\u2012CRISPR\/Cas12a platform for early short-beak and dwarfism syndrome virus nucleic acids detection","volume":"104","author":"Chen","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0005","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1080\/03079450802484987","article-title":"Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms","volume":"37","author":"Feberwee","year":"2008","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0006","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1080\/03079450802662772","article-title":"Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies","volume":"38","author":"Feberwee","year":"2009","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0007","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.psj.2025.106126_bib0008","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.113994","article-title":"A one-pot CRISPR\/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics","volume":"202","author":"Hu","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.psj.2025.106126_bib0009","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s12985-015-0255-y","article-title":"A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae","volume":"12","author":"Huang","year":"2015","journal-title":"Virol. J."},{"key":"10.1016\/j.psj.2025.106126_bib0010","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.2460\/ajvr.1972.33.10.2037","article-title":"Decrease of antibody response to Mycoplasma synoviae in chickens infected with Marek's disease herpesvirus","volume":"33","author":"Kleven","year":"1972","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.psj.2025.106126_bib0011","doi-asserted-by":"crossref","first-page":"126","DOI":"10.2307\/1588963","article-title":"Influence of strain of Mycoplasma synoviae and route of infection on development of synovitis or airsacculitis in broilers","volume":"19","author":"Kleven","year":"1975","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2025.106126_bib0012","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/03079457.2014.881049","article-title":"Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum? A viewpoint from the Netherlands","volume":"43","author":"Landman","year":"2014","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0013","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/03079457.2011.652595","article-title":"Longitudinal field study on the occurrence of Mycoplasma synoviae in Dutch turkey flocks with lameness and experimental induction of the condition","volume":"41","author":"Landman","year":"2012","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0014","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/s41422-018-0022-x","article-title":"CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA","volume":"28","author":"Li","year":"2018","journal-title":"Cell Res."},{"key":"10.1016\/j.psj.2025.106126_bib0015","article-title":"Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes","volume":"11","author":"Li","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.psj.2025.106126_bib0016","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1177\/030098589803500303","article-title":"Pathogenicity of Mycoplasma synoviae in broiler chickens","volume":"35","author":"Lockaby","year":"1998","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0017","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1080\/03079457.2016.1145354","article-title":"Prevalence of Mycoplasma gallisepticum and Mycoplasma synoviae in commercial poultry, racing pigeons and wild birds in Belgium","volume":"45","author":"Michiels","year":"2016","journal-title":"Avian Pathol."},{"key":"10.1016\/j.psj.2025.106126_bib0018","first-page":"239","article-title":"Diagnosis of avian mycoplasmas: a comparison between PCR and culture technique","volume":"73","author":"Muhammad","year":"2018","journal-title":"Arch. Razi. Inst."},{"key":"10.1016\/j.psj.2025.106126_bib0019","doi-asserted-by":"crossref","first-page":"4906","DOI":"10.1038\/s41467-020-18615-1","article-title":"Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection","volume":"11","author":"Nguyen","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.psj.2025.106126_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105552","article-title":"Prevalence and antimicrobial susceptibility of Mycoplasma gallisepticum and Mycoplasma synoviae isolated from the central peninsular Malaysia","volume":"104","author":"Rufai","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0021","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101522","article-title":"Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019","volume":"101","author":"Sui","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0022","article-title":"Fast and sensitive differential diagnosis of pseudorabies virus-infected versus pseudorabies virus-vaccinated swine using CRISPR-Cas12a","volume":"12","author":"Wang","year":"2024","journal-title":"Microbiol. Spectr."},{"issue":"2","key":"10.1016\/j.psj.2025.106126_bib0023","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3390\/v14020179","article-title":"Rapid detection of genotype II African Swine Fever virus using CRISPR Cas13a-based lateral flow strip","volume":"14","author":"Wei","year":"2022","journal-title":"Viruses."},{"key":"10.1016\/j.psj.2025.106126_bib0024","article-title":"Epidemiological investigations and multilocus sequence typing of Mycoplasma synoviae isolates from chicken farms in China","volume":"102","author":"Wei","year":"2023","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.103995","article-title":"Research note: real-time fluorescence-based recombinase-aided amplification for rapid detection of Mycoplasma synoviae","volume":"103","author":"Xia","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0026","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1080\/10495398.2021.1908316","article-title":"Insights on Mycoplasma gallisepticum and Mycoplasma synoviae infection in poultry: a systematic review","volume":"33","author":"Yadav","year":"2022","journal-title":"Anim. Biotechnol."},{"key":"10.1016\/j.psj.2025.106126_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115839","article-title":"The design strategies for CRISPR-based biosensing: target recognition, signal conversion, and signal amplification","volume":"246","author":"Yin","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.psj.2025.106126_bib0028","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104048","article-title":"A rapid, ultrasensitive, and highly specific method for detecting fowl adenovirus serotype 4 based on the LAMP-CRISPR\/Cas12a system","volume":"103","author":"Yu","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2025.106126_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105860","article-title":"Research note: prevalence and vlhA gene genotyping analysis of Mycoplasma synoviae in poultry from 2021 to 2023 in central China","volume":"104","author":"Zhang","year":"2025","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125013665?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579125013665?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T16:00:09Z","timestamp":1773331209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579125013665"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S0032579125013665"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2025.106126","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid nucleic acid detection of Mycoplasma synoviae using dual-mode RAA\u2013CRISPR\/Cas12a system","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2025.106126","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"106126"}}