{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T20:57:44Z","timestamp":1792357064645,"version":"4.4.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:00:00Z","timestamp":1768608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32502995"],"award-info":[{"award-number":["32502995"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013805","name":"Shanxi Agricultural University","doi-asserted-by":"publisher","award":["J202111305"],"award-info":[{"award-number":["J202111305"]}],"id":[{"id":"10.13039\/501100013805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013317","name":"Shanxi Provincial Key Research and Development Project","doi-asserted-by":"publisher","award":["2022ZDYF126"],"award-info":[{"award-number":["2022ZDYF126"]}],"id":[{"id":"10.13039\/501100013317","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.psj.2026.106466","type":"journal-article","created":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T15:40:41Z","timestamp":1768750841000},"page":"106466","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["The construction of recombinant DNA vaccine pVAX-ROP27-IL-2-IFN-\u03b3 and its immune enhancement effect against Eimeria tenella"],"prefix":"10.1016","volume":"105","author":[{"given":"Zhibin","family":"Niu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewei","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuihao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengbo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-gang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhen","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxue","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1676-9917","authenticated-orcid":false,"given":"Xiaoling","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.106466_bib0001","doi-asserted-by":"crossref","first-page":"e1469","DOI":"10.1002\/vms3.1469","article-title":"The global prevalence and associated risk factors of Eimeria infection in domestic chickens: a systematic review and meta-analysis","volume":"10","author":"Badri","year":"2024","journal-title":"Vet. Med. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0002","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.3382\/ps\/pew159","article-title":"Immune responses to improving welfare","volume":"95","author":"Berghman","year":"2016","journal-title":"Poult. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0003","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1007\/s00436-022-07499-3","article-title":"Characterization of the Eimeria tenella rhoptry protein with a nuclear localization sequence (EtROP30)","volume":"121","author":"Bingxiang","year":"2022","journal-title":"Parasitol. Res. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0004","article-title":"Kinetics of the cellular and transcriptomic response to eimeria maxima in relatively resistant and susceptible chicken lines","volume":"12","author":"Bremner","year":"2021","journal-title":"Front. Immunol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0005","first-page":"39","article-title":"Effects of eimeria tenella on cecal luminal and mucosal microbiota in broiler chickens","volume":"66","author":"Campos","year":"2022","journal-title":"Avian. Dis. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0006","article-title":"Effect of natural garlic essential oil on chickens with artificially infected Eimeria tenella","volume":"300","author":"Chang","year":"2021","journal-title":"Vet. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0007","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/S0020-7519(98)00024-1","article-title":"Evaluation of the efficacy of anticoccidial drugs against Eimeria species in the fowl","volume":"28","author":"Chapman","year":"1998","journal-title":"Int. J. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0008","article-title":"IFN-\u03b3 inhibitory molecules derived from Eimeria maxima inhibit IL-12 secretion by modulating MAPK pathways in chicken macrophages","volume":"103","author":"Chen","year":"2024","journal-title":"Poult. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0009","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s13567-023-01253-y","article-title":"Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens","volume":"54","author":"Chen","year":"2023","journal-title":"Vet. Res. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0010","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1080\/03079459008418702","article-title":"Relationship of coccidial lesion scores and weight gain in infections of eimeria acervulina, E. maxima and E. tenella in broilers","volume":"19","author":"Conway","year":"1990","journal-title":"Avian. Pathol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0011","article-title":"Eimeria tenella ROP kinase EtROP1 induces G0\/G1 cell cycle arrest and inhibits host cell apoptosis","volume":"21","author":"Diallo","year":"2019","journal-title":"Cell Microbiol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0012","article-title":"Transgenic Eimeria parasite: a potential control strategy for chicken coccidiosis","volume":"205","author":"Fatoba","year":"2020","journal-title":"Acta Trop [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0013","first-page":"2033","article-title":"Maternal antibody persistence: a neglected life-history trait with implications from albatross conservation to comparative immunology","volume":"279","author":"Garnier","year":"2012","journal-title":"Proc. Biol. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0014","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.coi.2020.01.006","article-title":"DNA vaccines: prime time is now","volume":"65","author":"Gary","year":"2020","journal-title":"Curr. Opin. Immunol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0015","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.vetpar.2010.11.041","article-title":"Vaccination of chickens with DNA vaccine expressing eimeria tenella MZ5-7 against coccidiosis","volume":"177","author":"Geriletu","year":"2011","journal-title":"Vet. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0016","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0304-4017(00)00275-2","article-title":"Inhibition of Eimeria tenella replication after recombinant IFN-gamma activation in chicken macrophages, fibroblasts and epithelial cells","volume":"92","author":"H\u00e9riveau","year":"2000","journal-title":"Vet. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0017","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0165-2427(01)00414-7","article-title":"The emerging role of avian cytokines as immunotherapeutics and vaccine adjuvants","volume":"85","author":"Hilton","year":"2002","journal-title":"Vet. Immunol. Immunopathol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0018","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/0014-4894(70)90063-9","article-title":"Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens","volume":"28","author":"Johnson","year":"1970","journal-title":"Exp. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0019","article-title":"Immunohistochemical identification of T and B lymphocytes in formalin-fixed paraffin-embedded chicken tissues using commercial antibodies","volume":"228","author":"Kurokawa","year":"2020","journal-title":"Vet. Immunol. Immunopathol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0020","article-title":"Recombinant ubiquitin-conjugating enzyme of Eimeria maxima induces immunogenic maturation in chicken splenic-derived dendritic cells and drives Th1 polarization in-vitro","volume":"143","author":"Lakho","year":"2020","journal-title":"Microb. Pathog. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0021","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s00436-020-07016-4","article-title":"Actin-depolymerizing factor from Eimeria tenella promotes immunogenic function of chicken dendritic cells","volume":"120","author":"Lakho","year":"2021","journal-title":"Parasitol. Res. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0022","article-title":"Co-administration of chicken IL-7 or NK-lysin peptide 2 enhances the efficacy of Eimeria elongation factor-1alpha vaccination against Eimeria maxima infection in broiler chickens","volume":"101","author":"Lee","year":"2022","journal-title":"Poult. Sci. [J]"},{"issue":"22","key":"10.1016\/j.psj.2026.106466_bib0023","doi-asserted-by":"crossref","first-page":"3500","DOI":"10.3390\/ani13223500","article-title":"The Immunoprotective Effect of ROP27 Protein of Eimeria tenella","volume":"13","author":"Li","year":"2023","journal-title":"Animals (Basel)"},{"key":"10.1016\/j.psj.2026.106466_bib0024","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.pt.2023.09.002","article-title":"Live attenuated anticoccidial vaccines for chickens","volume":"39","author":"Liu","year":"2023","journal-title":"Trends Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0025","first-page":"201","article-title":"A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis","volume":"6","author":"Long","year":"1976","journal-title":"Folia. Vet. Lat. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0026","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.exppara.2010.07.015","article-title":"Vaccination of chickens with DNA vaccine encoding eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge","volume":"127","author":"Ma","year":"2011","journal-title":"Exp. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0027","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1016\/S0264-410X(97)00088-1","article-title":"DNA vaccination with cytokine fusion constructs biases the immune response to ovalbumin","volume":"15","author":"Maecker","year":"1997","journal-title":"Vaccine [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0028","article-title":"DNA vaccines: history, molecular mechanisms and future perspectives","volume":"435","author":"Pagliari","year":"2023","journal-title":"J. Mol. Biol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0029","article-title":"Relationship between Eimeria tenella associated-early clinical signs and molecular changes in the intestinal barrier function","volume":"240","author":"Pham","year":"2021","journal-title":"Vet. Immunol. Immunopathol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0030","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.vetimm.2011.07.006","article-title":"Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-\u03b3 and IL-4 genes","volume":"144","author":"Sawant","year":"2011","journal-title":"Vet. Immunol. Immunopathol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0031","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.rvsc.2010.05.003","article-title":"Construction of DNA vaccines encoding eimeria acervulina cSZ-2 with chicken IL-2 and IFN-gamma and their efficacy against poultry coccidiosis","volume":"90","author":"Shah","year":"2011","journal-title":"Res. Vet. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0032","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1007\/s00436-010-1940-9","article-title":"The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis","volume":"107","author":"Shah","year":"2010","journal-title":"Parasitol. Res. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0033","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.exppara.2009.11.010","article-title":"Cross immunity of DNA vaccine pVAX1-cSZ2-IL-2 to Eimeria tenella, E. necatrix and E. maxima","volume":"124","author":"Shah","year":"2010","journal-title":"Exp. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0034","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.rvsc.2012.11.006","article-title":"The protective efficacy of chimeric SO7\/IL-2 DNA vaccine against coccidiosis in chickens","volume":"94","author":"Song","year":"2013","journal-title":"Res. Vet. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0035","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.vetpar.2015.08.026","article-title":"Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine","volume":"212","author":"Song","year":"2015","journal-title":"Vet. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0036","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3389\/fvets.2020.00101","article-title":"Poultry coccidiosis: design and interpretation of vaccine studies","volume":"7","author":"Soutter","year":"2020","journal-title":"Front. Vet. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0037","article-title":"Immune synergistic mechanism of recombinant plasmid adjuvant containing chicken IL-4 and IL-2 fusion genes on chicken coccidia live vaccine","volume":"103","author":"Tan","year":"2024","journal-title":"Poult. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0038","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.pt.2010.04.004","article-title":"Role of antibody in immunity and control of chicken coccidiosis","volume":"26","author":"Wallach","year":"2010","journal-title":"Trends Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0040","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3347\/kjp.2014.52.3.251","article-title":"A novel recombinant BCG vaccine encoding eimeria tenella rhomboid and chicken IL-2 induces protective immunity against coccidiosis","volume":"52","author":"Wang","year":"2014","journal-title":"Korean J. Parasitol. [J]"},{"issue":"9","key":"10.1016\/j.psj.2026.106466_bib0039","first-page":"465","article-title":"Identification and Characterization of Eimeria tenella Rhoptry Protein 35 (EtROP35)","volume":"9","author":"Wang","year":"2022","journal-title":"Vet Sci"},{"key":"10.1016\/j.psj.2026.106466_bib0041","article-title":"Molecular characterization of methionine aminopeptidase1 from Eimeria tenella","volume":"90","author":"Xiao","year":"2023","journal-title":"Eur. J. Protistol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0042","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.vetpar.2008.05.025","article-title":"Vaccination of chickens with a chimeric DNA vaccine encoding eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis","volume":"156","author":"Xu","year":"2008","journal-title":"Vet. Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0043","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1637\/7535-032706R1.1","article-title":"Protective immunity enhanced by chimeric DNA prime-protein booster strategy against Eimeria tenella challenge","volume":"50","author":"Xu","year":"2006","journal-title":"Avian. Dis. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0044","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13567-022-01045-w","article-title":"A multiepitope vaccine encoding four eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens","volume":"53","author":"Yu","year":"2022","journal-title":"Vet. Res. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0045","doi-asserted-by":"crossref","first-page":"745","DOI":"10.2478\/s11686-019-00090-4","article-title":"Optimization of immunization procedure for Eimeria tenella DNA vaccine pVAX1-pEtK2-IL-2 and its stability","volume":"64","author":"Zhang","year":"2019","journal-title":"Acta Parasitol. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0046","article-title":"Development and evaluation of protective immunity of a ROP27 DNA vaccine against Eimeria tenella in chickens","volume":"104","author":"Zhao","year":"2025","journal-title":"Poult. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0047","article-title":"Prevention and control of chicken coccidiosis construction of recombinant lactococcus lactis expressing chicken IL-4 and IL-2 fusion protein and its immune synergistic effect on chicken coccidia live vaccine","volume":"102","author":"Zheng","year":"2023","journal-title":"Poult. Sci. [J]"},{"key":"10.1016\/j.psj.2026.106466_bib0048","article-title":"Impacts of Eimeria coinfection on growth performance, intestinal health and immune responses of broiler chickens","volume":"322","author":"Zhou","year":"2023","journal-title":"Vet. Parasitol. [J]"}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126000933?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126000933?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:57:43Z","timestamp":1777449463000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126000933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S0032579126000933"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.106466","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The construction of recombinant DNA vaccine pVAX-ROP27-IL-2-IFN-\u03b3 and its immune enhancement effect against Eimeria tenella","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.106466","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"106466"}}