{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T17:39:34Z","timestamp":1781545174982,"version":"3.54.5"},"reference-count":51,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.psj.2026.106623","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:01:06Z","timestamp":1770912066000},"page":"106623","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dietary evaluation of Pleurotus ostreatus spent mushroom substrate on productivity, nutrient digestibility, egg quality, antioxidant status, and ovarian cytoprotective responses in laying hens"],"prefix":"10.1016","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0499-1564","authenticated-orcid":false,"given":"Ioannis","family":"Brouklogiannis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9786-7353","authenticated-orcid":false,"given":"Georgios","family":"Koutrotsios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios I.","family":"Zervakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7926-5604","authenticated-orcid":false,"given":"Konstantinos C.","family":"Mountzouris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.106623_bib0001","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1637147","article-title":"Utilizing brown mushroom stem waste as an eco-friendly alternative to soybean meal in layer chick nutrition","volume":"12","author":"Abdel-Wareth","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0002","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.bbadis.2016.11.005","article-title":"Nrf2 signaling pathway: pivotal roles in inflammation","volume":"1863","author":"Ahmed","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"10.1016\/j.psj.2026.106623_bib0003","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3390\/ani13020294","article-title":"Phytogenic effects on layer production performance and cytoprotective response in the duodenum","volume":"13","author":"Anagnostopoulos","year":"2023","journal-title":"Animals"},{"key":"10.1016\/j.psj.2026.106623_bib0004","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.toxlet.2015.05.020","article-title":"Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)","volume":"237","author":"Antos","year":"2015","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.psj.2026.106623_bib0005","first-page":"183","article-title":"Effects of dietary spent mushroom (Cordyceps militaris) substrate supplements on productive performance and egg yolk cholesterol of laying hens","volume":"1","author":"Arjin","year":"2015","journal-title":"Thai J. Anim. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0006","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.anifeedsci.2017.06.001","article-title":"The use of basidiomycota mushrooms in poultry nutrition\u2014A review","volume":"230","author":"Bederska-\u0141ojewska","year":"2017","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.1016\/j.psj.2026.106623_bib0007","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3390\/foods9040535","article-title":"Pleurotus mushrooms content in glucans and ergosterol assessed by ATR-FTIR spectroscopy and multivariate analysis","volume":"9","author":"Bekiaris","year":"2020","journal-title":"Foods"},{"key":"10.1016\/j.psj.2026.106623_bib0008","first-page":"143","article-title":"The effect of feeding rosemary, oregano, saffron and \u03b1-tocopheryl acetate on hen performance and oxidative stability of eggs","volume":"35","author":"Botsoglou","year":"2005","journal-title":"S. Afr. J. Anim. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.102508","article-title":"Dietary phytogenic inclusion level affects production performance and expression of ovarian cytoprotective genes in laying hens","volume":"102","author":"Brouklogiannis","year":"2023","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0010","doi-asserted-by":"crossref","DOI":"10.1080\/10495398.2025.2463995","article-title":"Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance","volume":"36","author":"Brouklogiannis","year":"2025","journal-title":"Anim. Biotechnol."},{"key":"10.1016\/j.psj.2026.106623_bib0011","doi-asserted-by":"crossref","DOI":"10.1016\/j.livsci.2025.105749","article-title":"Hepatic cytoprotective and immunomodulatory responses to dietary phytogenic inclusion level in laying hens","volume":"298","author":"Brouklogiannis","year":"2025","journal-title":"Livest. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0012","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3184\/175815511X12919999481888","article-title":"Beta-glucans as immunomodulators in poultry: use and potential applications","volume":"3","author":"Cox","year":"2010","journal-title":"Avian Biol. Res."},{"key":"10.1016\/j.psj.2026.106623_bib0013","doi-asserted-by":"crossref","DOI":"10.1016\/j.animal.2022.100543","article-title":"Review: implication of redox imbalance in animal health and performance at critical periods, insights from different farm species","volume":"16","author":"Durand","year":"2022","journal-title":"Animal"},{"key":"10.1016\/j.psj.2026.106623_bib0014","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.3390\/ani11041022","article-title":"Dietary pomegranate by-product alleviated oxidative stress induced by dexamethasone in laying hens in the pre-peak period","volume":"11","author":"Eid","year":"2021","journal-title":"Animals"},{"key":"10.1016\/j.psj.2026.106623_bib0015","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1600-065X.2009.00793.x","article-title":"\u03b2-glucan recognition by the innate immune system","volume":"230","author":"Goodridge","year":"2009","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.psj.2026.106623_bib0016","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1002\/bbb.2544","article-title":"Progress in lignocellulosic biomass valorization for biofuels and value-added chemical production in the EU: a focus on thermochemical conversion processes","volume":"18","author":"G\u00fcle\u00e7","year":"2024","journal-title":"Biofuels Bioprod. Bioref."},{"key":"10.1016\/j.psj.2026.106623_bib0017","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2007-8-2-r19","article-title":"qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data","volume":"8","author":"Hellemans","year":"2008","journal-title":"Genome Biol"},{"key":"10.1016\/j.psj.2026.106623_bib0018","first-page":"146","article-title":"Effect of shiitake mushroom [Lentinula edodes (Berk.) Pegler] on laying performance, egg quality, fatty acid composition and cholesterol concentration of eggs in layer chickens","volume":"6","author":"Hwang","year":"2012","journal-title":"J. Med. Plants Res."},{"key":"10.1016\/j.psj.2026.106623_bib0019","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3844\/ajisp.2017.45.49","article-title":"Glucans and the poultry immune system","volume":"13","author":"Jacob","year":"2017","journal-title":"Am. J. Immunol."},{"key":"10.1016\/j.psj.2026.106623_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104320","article-title":"The influence of dietary supplementation with oyster mushroom waste on laying hens\u2019 performance, egg quality and immune parameters","volume":"103","author":"Karageorgou","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0021","doi-asserted-by":"crossref","first-page":"350","DOI":"10.14480\/JM.2014.12.4.350","article-title":"Effect of dietary fermented spent mushroom substrates on laying hens","volume":"12","author":"Kim","year":"2014","journal-title":"J. Mushroom"},{"key":"10.1016\/j.psj.2026.106623_bib0022","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.3382\/ps\/pey087","article-title":"Inflammatory phenotypes in the intestine of poultry: not all inflammation is created equal","volume":"97","author":"Kogut","year":"2018","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0023","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.foodchem.2014.03.121","article-title":"Bioconversion of lignocellulosic residues by agrocybe cylindracea and pleurotus ostreatus mushroom fungi-assessment of their effect on the final product and spent substrate properties","volume":"161","author":"Koutrotsios","year":"2014","journal-title":"Food Chem"},{"key":"10.1016\/j.psj.2026.106623_bib0024","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1007\/s11274-017-2262-1","article-title":"Bioactive compounds and antioxidant activity exhibit high intraspecific variability in Pleurotus ostreatus mushrooms and correlate well with cultivation performance parameters","volume":"33","author":"Koutrotsios","year":"2017","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.psj.2026.106623_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biopen.2018.05.001","article-title":"AhR signaling pathways and regulatory functions","volume":"7","author":"Larigot","year":"2018","journal-title":"Biochim. Open"},{"key":"10.1016\/j.psj.2026.106623_bib0026","doi-asserted-by":"crossref","first-page":"4240","DOI":"10.3382\/ps\/pey407","article-title":"From oxidative stress to inflammation: redox balance and immune system","volume":"98","author":"Lauridsen","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0027","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.3382\/ps.2009-00063","article-title":"Effect of irradiation on foaming properties of egg white proteins","volume":"88","author":"Liu","year":"2009","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0028","doi-asserted-by":"crossref","DOI":"10.1080\/21655979.2023.2252138","article-title":"Spent substrate from mushroom cultivation: exploitation potential toward various applications and value-added products","volume":"14","author":"Mart\u00edn","year":"2023","journal-title":"Bioengineered"},{"key":"10.1016\/j.psj.2026.106623_bib0029","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3382\/japr.2011-00477","article-title":"Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics","volume":"21","author":"Mateos","year":"2012","journal-title":"J. Appl. Poult. Res."},{"key":"10.1016\/j.psj.2026.106623_bib0030","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1093\/ps\/84.11.1791","article-title":"Effect of irradiating shell eggs on quality attributes and functional properties of yolk and white","volume":"84","author":"Min","year":"2005","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0031","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cr.2010.178","article-title":"Crosstalk of reactive oxygen species and NF-\u03bab signaling","volume":"21","author":"Morgan","year":"2011","journal-title":"Cell Res"},{"key":"10.1016\/j.psj.2026.106623_bib0032","doi-asserted-by":"crossref","DOI":"10.1016\/j.livsci.2024.105525","article-title":"Phytogenics as natural gut health management tools for sustainable poultry production","volume":"286","author":"Mountzouris","year":"2024","journal-title":"Livest. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0033","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3382\/ps.0190163","article-title":"The porosity of the egg-shell in relation to hatchability","volume":"19","author":"Mueller","year":"1940","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0034","doi-asserted-by":"crossref","DOI":"10.1088\/1755-1315\/478\/1\/012085","article-title":"Effect of white oyster mushroom (Pleurotus ostreatus) flour addition on laying hens performance","volume":"478","author":"Natsir","year":"2020","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0035","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.3923\/ijps.2009.1052.1057","article-title":"Potential of a wild medicinal mushroom, ganoderma Sp., as feed supplement in chicken diet: effect on performance and health of pullets","volume":"8","author":"Ogbe","year":"2009","journal-title":"Int. J. Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0036","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.3382\/ps\/pey588","article-title":"Modulation of broiler gut microbiota and gene expression of toll-like receptors and tight junction proteins by diet type and inclusion of phytogenics","volume":"98","author":"Paraskeuas","year":"2019","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0037","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.3390\/ani13193096","article-title":"Dietary inclusion level effects of yoghurt acid whey powder on performance, digestibility of nutrients and meat quality of broilers","volume":"13","author":"Paraskeuas","year":"2023","journal-title":"Animals"},{"key":"10.1016\/j.psj.2026.106623_bib0038","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.psj.2026.106623_bib0039","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/s00253-012-4446-9","article-title":"Potential uses of spent mushroom substrate and its associated lignocellulosic enzymes","volume":"96","author":"Phan","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.psj.2026.106623_bib0040","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/0048-4059(72)90043-4","article-title":"A rapid method for the chemical estimation of filamentous fungi in plant tissue","volume":"2","author":"Ride","year":"1972","journal-title":"Physiol. Plant Pathol."},{"key":"10.1016\/j.psj.2026.106623_bib0041","series-title":"Edible and Medicinal Mushrooms: Technology and Applications","first-page":"5","article-title":"Current overview of mushroom production in the world","author":"Royse","year":"2017"},{"key":"10.1016\/j.psj.2026.106623_bib0042","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1369-703X(00)00090-5","article-title":"Glucosamine measurement as indirect method for biomass estimation of cunninghamella elegans grown in solid state cultivation conditions","volume":"7","author":"Scotti","year":"2001","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.psj.2026.106623_bib0043","doi-asserted-by":"crossref","first-page":"3730","DOI":"10.1039\/D4SU00342J","article-title":"Lignocellulosic biomass valorisation: a review of feedstocks, processes and potential value chains and their implications for the decision-making process","volume":"2","author":"Segers","year":"2024","journal-title":"RSC Sustain."},{"key":"10.1016\/j.psj.2026.106623_bib0044","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3390\/ani11010181","article-title":"Effects of dietary fiber on nutrients utilization and gut health of poultry: a review of challenges and opportunities","volume":"11","author":"Singh","year":"2021","journal-title":"Animals"},{"key":"10.1016\/j.psj.2026.106623_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105571","article-title":"Utilizing white mushroom stem powder as a sustainable substitute for soybean meal in layer chick diets","volume":"104","author":"Stamps","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0046","doi-asserted-by":"crossref","first-page":"663","DOI":"10.3390\/agriculture14050663","article-title":"Pleurotus ostreatus mushroom: a promising feed supplement in poultry farming","volume":"14","author":"T\u00f6r\u0151s","year":"2024","journal-title":"Agriculture"},{"key":"10.1016\/j.psj.2026.106623_bib0047","first-page":"243","article-title":"Potential roles of the aryl hydrocarbon receptor in female reproductive senescence","volume":"4","author":"Valdez","year":"2004","journal-title":"Reprod. Biol."},{"key":"10.1016\/j.psj.2026.106623_bib0048","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.3390\/ijms18122772","article-title":"Nrf2, the master regulator of anti-oxidative responses","volume":"18","author":"Vomund","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.psj.2026.106623_bib0049","doi-asserted-by":"crossref","first-page":"256","DOI":"10.5713\/ajas.19.0853","article-title":"Effects of the Agaricus bisporus stem residue on performance, nutrients digestibility and antioxidant activity of laying hens and its effects on egg storage","volume":"34","author":"Yang","year":"2021","journal-title":"Anim. Biosci."},{"key":"10.1016\/j.psj.2026.106623_bib0050","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.1080\/09168451.2017.1383846","article-title":"Effect of dietary fermented mushroom bed on egg production in laying hens","volume":"81","author":"Yoshida","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.psj.2026.106623_bib0051","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.102033","article-title":"Shudi Erzi San relieves ovary aging in laying hens","volume":"101","author":"Zhang","year":"2022","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126002506?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126002506?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T14:31:15Z","timestamp":1777300275000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126002506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["S0032579126002506"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.106623","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dietary evaluation of Pleurotus ostreatus spent mushroom substrate on productivity, nutrient digestibility, egg quality, antioxidant status, and ovarian cytoprotective responses in laying hens","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.106623","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"106623"}}