{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:30:55Z","timestamp":1792373455453,"version":"4.4.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800200"],"award-info":[{"award-number":["2023YFD1800200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012438","name":"Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System","doi-asserted-by":"publisher","award":["SCCXTD-2021-18"],"award-info":[{"award-number":["SCCXTD-2021-18"]}],"id":[{"id":"10.13039\/501100012438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-42-17"],"award-info":[{"award-number":["CARS-42-17"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.psj.2026.106651","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T16:49:02Z","timestamp":1771433342000},"page":"106651","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Attenuation mechanisms and vaccine potential of the serial passage\u2013derived Pasteurella multocida strain PMZ8 in ducks"],"prefix":"10.1016","volume":"105","author":[{"given":"Xiangfei","family":"Ji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xumin","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingshu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.106651_bib0001","doi-asserted-by":"crossref","first-page":"590","DOI":"10.2307\/1589017","article-title":"Tissue irritation induced in turkeys by fowl cholera bacterins","volume":"18","author":"Ahmed","year":"1974","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2026.106651_bib0002","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.envres.2018.11.040","article-title":"Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: a review","volume":"169","author":"Ben","year":"2019","journal-title":"Environ. Res."},{"key":"10.1016\/j.psj.2026.106651_bib0003","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/0003-2697(62)90095-7","article-title":"A modified uronic acid carbazole reaction","volume":"4","author":"Bitter","year":"1962","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.psj.2026.106651_bib0004","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1751-0813.1995.tb15033.x","article-title":"Characterisation of pasteurella multocida isolated from fowl cholera outbreaks on turkey farms","volume":"72","author":"Blackall","year":"1995","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.psj.2026.106651_bib0005","doi-asserted-by":"crossref","first-page":"6124","DOI":"10.1128\/AEM.00809-09","article-title":"New plasmid tools for genetic analysis of Actinobacillus pleuropneumoniae and other pasteurellaceae","volume":"75","author":"Boss\u00e9","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0006","doi-asserted-by":"crossref","first-page":"16","DOI":"10.2307\/1590943","article-title":"Epidemiology and financial impact of fowl cholera in turkeys: a retrospective analysis","volume":"32","author":"Carpenter","year":"1988","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2026.106651_bib0007","first-page":"473","article-title":"Dissociation and virulence in strains of Pasteurella Multocida isolated from a variety of lesions","volume":"17","author":"Carter","year":"1953","journal-title":"Can. J. Comp. Med. Vet. Sci."},{"key":"10.1016\/j.psj.2026.106651_bib0008","doi-asserted-by":"crossref","first-page":"2751","DOI":"10.1016\/j.vaccine.2004.11.036","article-title":"Vaccination against fowl cholera with acapsular Pasteurella multocida A:1","volume":"23","author":"Chung","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.psj.2026.106651_bib0009","doi-asserted-by":"crossref","first-page":"2487","DOI":"10.1128\/IAI.69.4.2487-2492.2001","article-title":"Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A","volume":"69","author":"Chung","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.psj.2026.106651_bib0010","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1007\/s000180050461","article-title":"Hyaluronan synthases: fascinating glycosyltransferases from vertebrates, bacterial pathogens, and algal viruses","volume":"56","author":"DeAngelis","year":"1999","journal-title":"Cell Mol. Life Sci."},{"key":"10.1016\/j.psj.2026.106651_bib0011","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.tim.2013.11.008","article-title":"Bacterial virulence and fis: adapting regulatory networks to the host environment","volume":"22","author":"Duprey","year":"2014","journal-title":"Trends. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0012","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0378-1119(97)00619-7","article-title":"Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression","volume":"207","author":"Edwards","year":"1998","journal-title":"Gene"},{"key":"10.1016\/j.psj.2026.106651_bib0013","doi-asserted-by":"crossref","first-page":"7727","DOI":"10.1074\/jbc.M115.701540","article-title":"Structural studies of lipopolysaccharide-defective mutants from Brucella melitensis identify a core oligosaccharide critical in virulence","volume":"291","author":"Fontana","year":"2016","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.psj.2026.106651_bib0014","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104380","article-title":"Molecular pathogenesis of the hyaluronic acid capsule of Pasteurella multocida","volume":"149","author":"Guan","year":"2020","journal-title":"Microb. Pathogen."},{"key":"10.1016\/j.psj.2026.106651_bib0015","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0378-1135(89)90030-8","article-title":"Serum resistance is correlated with encapsulation of avian strains of pasteurella multocida","volume":"21","author":"Hansen","year":"1989","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0016","doi-asserted-by":"crossref","first-page":"254","DOI":"10.3390\/toxins9080254","article-title":"The myriad properties of Pasteurella multocida lipopolysaccharide","volume":"9","author":"Harper","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.psj.2026.106651_bib0017","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.vetmic.2011.05.022","article-title":"Pasteurella multocida lipopolysaccharide: the long and the short of it","volume":"153","author":"Harper","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0018","doi-asserted-by":"crossref","first-page":"7384","DOI":"10.1128\/JB.00948-07","article-title":"Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence","volume":"189","author":"Harper","year":"2007","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.psj.2026.106651_bib0019","doi-asserted-by":"crossref","first-page":"3436","DOI":"10.1128\/IAI.72.6.3436-3443.2004","article-title":"A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence","volume":"72","author":"Harper","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.psj.2026.106651_bib0020","doi-asserted-by":"crossref","first-page":"925","DOI":"10.2307\/1588773","article-title":"Fowl cholera: gel diffusion precipitin test for serotyping pasteruella multocida from avian species","volume":"16","author":"Heddleston","year":"1972","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2026.106651_bib0021","doi-asserted-by":"crossref","first-page":"317","DOI":"10.2307\/1592184","article-title":"Comparison of live avirulent PM-1 and CU fowl cholera vaccines in turkeys","volume":"41","author":"Hopkins","year":"1997","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2026.106651_bib0022","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.3390\/microorganisms13051024","article-title":"Characterization of a capsule-deficient Pasteurella multocida isolated from Cygnus melancoryphus: genomic, phenotypic, and virulence insights","volume":"13","author":"Jiang","year":"2025","journal-title":"Microorganisms."},{"key":"10.1016\/j.psj.2026.106651_bib0023","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.1128\/JCM.43.6.2959-2961.2005","article-title":"Molecular epidemiology investigation of outbreaks of fowl cholera in geographically related poultry flocks","volume":"43","author":"Kardos","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0024","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0165-022X(93)90024-I","article-title":"Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels","volume":"26","author":"Kittelberger","year":"1993","journal-title":"J. Biochem. Bioph. Methods"},{"key":"10.1016\/j.psj.2026.106651_bib0025","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.2307\/1590284","article-title":"Efficacy of broth-grown pasteurella multocida bacterins in ducklings","volume":"28","author":"Layton","year":"1984","journal-title":"Avian Dis."},{"key":"10.1016\/j.psj.2026.106651_bib0026","doi-asserted-by":"crossref","first-page":"e3548","DOI":"10.21769\/BioProtoc.3548","article-title":"Contemporaneous measurement of outer and inner membrane permeability in gram-negative bacteria","volume":"10","author":"Ma","year":"2020","journal-title":"Bio-protocol"},{"key":"10.1016\/j.psj.2026.106651_bib0027","first-page":"140","article-title":"Pasteurella multocida vaccine candidates: a systematic review","volume":"12","author":"Mostaan","year":"2020","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"10.1016\/j.psj.2026.106651_bib0028","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1016\/S0264-410X(98)00046-2","article-title":"Inactivated whole-cell bacterial vaccines: current status and novel strategies","volume":"16","author":"Pace","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.psj.2026.106651_bib0029","doi-asserted-by":"crossref","DOI":"10.1128\/MMBR.00014-19","article-title":"Pasteurella multocida: genotypes and genomics","volume":"83","author":"Peng","year":"2019","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.psj.2026.106651_bib0030","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01843-17","article-title":"Capsular polysaccharide interferes with biofilm formation by pasteurella multocida serogroup a (Y Rikihisa, Ed.)","author":"Petruzzi","year":"2017","journal-title":"mBio."},{"key":"10.1016\/j.psj.2026.106651_bib0031","doi-asserted-by":"crossref","first-page":"7581","DOI":"10.1128\/jb.179.23.7581-7586.1997","article-title":"A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives","volume":"179","author":"Rubir\u00e9s","year":"1997","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.psj.2026.106651_bib0032","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1111\/febs.17061","article-title":"Ophthalmic acid is a glutathione regulating tripeptide","volume":"291","author":"Schomakers","year":"2024","journal-title":"FEBS. J."},{"key":"10.1016\/j.psj.2026.106651_bib0033","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000750","article-title":"Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors","volume":"6","author":"Steen","year":"2010","journal-title":"PLoS. Pathog."},{"key":"10.1016\/j.psj.2026.106651_bib0034","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1128\/IAI.70.7.3355-3362.2002","article-title":"Protective immunity conferred by attenuated aroA derivatives of Pasteurella multocida B:2 strains in a mouse model of hemorrhagic septicemia","volume":"70","author":"Tabatabaei","year":"2002","journal-title":"Infect. Immun."},{"key":"10.1016\/j.psj.2026.106651_bib0035","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/s12917-025-04769-x","article-title":"Deleting fis downregulates virulence and effectively protects Pasteurella multocida infection in mice","volume":"21","author":"Wang","year":"2025","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.psj.2026.106651_bib0036","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CMR.00024-13","article-title":"Pasteurella multocida: from zoonosis to cellular microbiology","volume":"26","author":"Wilson","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.psj.2026.106651_bib0037","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1637327","article-title":"Roles of SPI-2 T3SS effectors in virulence of salmonella choleraesuis and construction of a triple-gene mutant vaccine strain","volume":"12","author":"Xu","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2026.106651_bib0038","doi-asserted-by":"crossref","first-page":"391","DOI":"10.3389\/fcimb.2017.00391","article-title":"Two novel salmonella bivalent vaccines confer dual protection against two salmonella serovars in mice","volume":"7","author":"Zhao","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.psj.2026.106651_bib0039","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13567-021-00910-4","article-title":"The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks","volume":"52","author":"Zhao","year":"2021","journal-title":"Vet. Res."},{"key":"10.1016\/j.psj.2026.106651_bib0040","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s13567-022-01035-y","article-title":"Immunogenicity and protection of a pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks","volume":"53","author":"Zhao","year":"2022","journal-title":"Vet. Res."},{"key":"10.1016\/j.psj.2026.106651_bib0041","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.vaccine.2020.12.002","article-title":"Immunogenicity and protection efficacy of a salmonella enterica serovar typhimurium fnr, arcA and fliC mutant","volume":"39","author":"Zhao","year":"2021","journal-title":"Vaccine"}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126002786?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126002786?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:43:13Z","timestamp":1777441393000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126002786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["S0032579126002786"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.106651","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Attenuation mechanisms and vaccine potential of the serial passage\u2013derived Pasteurella multocida strain PMZ8 in ducks","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.106651","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"106651"}}