{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T23:45:45Z","timestamp":1782603945615,"version":"3.54.5"},"reference-count":29,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302738"],"award-info":[{"award-number":["32302738"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1300100"],"award-info":[{"award-number":["2022YFD1300100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["C2024402048"],"award-info":[{"award-number":["C2024402048"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.psj.2026.106821","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T10:07:11Z","timestamp":1773828431000},"page":"106821","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the uterine transcriptional mechanisms underlying eggshell strength through multi-stage analysis in laying hens"],"prefix":"10.1016","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0223-4751","authenticated-orcid":false,"given":"Liyuan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3833-459X","authenticated-orcid":false,"given":"Yanheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanwei","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiong","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6328-9660","authenticated-orcid":false,"given":"Xiaohui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahui","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.106821_bib0001","unstructured":"Andrews, S. 2010. FastQC: a quality control tool for high throughput sequence data. Available online at: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"10.1016\/j.psj.2026.106821_bib0002","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34,","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.psj.2026.106821_bib0003","article-title":"Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens","volume":"11","author":"Chen","year":"2021","journal-title":"Sci. Rep.-Uk"},{"key":"10.1016\/j.psj.2026.106821_bib0004","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.psj.2026.106821_bib0005","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.aninu.2023.03.004","article-title":"Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens","volume":"13","author":"Feng","year":"2023","journal-title":"Anim. Nutr."},{"key":"10.1016\/j.psj.2026.106821_bib0006","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1186\/s12864-020-07177-7","article-title":"Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens","volume":"21","author":"Feng","year":"2020","journal-title":"BMC Genom."},{"key":"10.1016\/j.psj.2026.106821_bib0007","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40104-023-00986-2","article-title":"Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens","volume":"15","author":"Fu","year":"2024","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.psj.2026.106821_bib0008","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.877739","article-title":"Genome-wide association study of egg-laying traits and egg quality in LingKun chickens","volume":"9","author":"Gao","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0009","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12860-021-00350-0","article-title":"Avian eggshell biomineralization: an update on its structure, mineralogy and protein tool kit","volume":"22","author":"Gautron","year":"2021","journal-title":"BMC Mol. Cell Biol."},{"key":"10.1016\/j.psj.2026.106821_bib0010","doi-asserted-by":"crossref","first-page":"105329","DOI":"10.1016\/j.psj.2025.105329","article-title":"Transcriptomic analysis of the liver, jejunum, and uterus in different production stages of laying hens","volume":"104","author":"Han","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0011","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.aninu.2025.03.024","article-title":"Disequilibrium feeding pattern more consistent with egg-laying physiology improves eggshell quality of laying hens during late laying period","volume":"23","author":"Hu","year":"2025","journal-title":"Anim. Nutr."},{"key":"10.1016\/j.psj.2026.106821_bib0012","doi-asserted-by":"crossref","first-page":"960","DOI":"10.3390\/antiox14080960","article-title":"Puerarin enhances eggshell quality by mitigating uterine senescence in late-phase laying breeder hens","volume":"14","author":"Huang","year":"2025","journal-title":"Antioxid.-Basel"},{"key":"10.1016\/j.psj.2026.106821_bib0013","first-page":"41","article-title":"Genomic-wide association study for egg weight-related traits in Rhode Island red breed using bayesian methods","volume":"11","author":"Khaltabadi Farahani","year":"2022","journal-title":"Anim. Prod. Res."},{"key":"10.1016\/j.psj.2026.106821_bib0014","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"Langfelder","year":"2008","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.psj.2026.106821_bib0015","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.psj.2026.106821_bib0016","article-title":"Effects of 28 h ahemeral light cycle on production performance, egg quality, blood parameters, and uterine characteristics of hens during the late laying period","volume":"103","author":"Liu","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0017","article-title":"Long-24-h ahemeral light cycle improved eggshell quality of hens in late laying period","volume":"104","author":"Liu","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0018","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.psj.2026.106821_bib0019","doi-asserted-by":"crossref","unstructured":"Nys, Y., and N. L.e Roy. 2018. Calcium homeostasis and eggshell biomineralization in female chicken. Pages 361\u2013382 in Vitamin dElsevier.","DOI":"10.1016\/B978-0-12-809965-0.00022-7"},{"key":"10.1016\/j.psj.2026.106821_bib0020","article-title":"RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization","volume":"8","author":"Sah","year":"2018","journal-title":"Sci. Rep.-Uk"},{"key":"10.1016\/j.psj.2026.106821_bib0021","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1002\/pmic.201300286","article-title":"Differential label-free quantitative proteomic analysis of avian eggshell matrix and uterine fluid proteins associated with eggshell mechanical property","volume":"13","author":"Sun","year":"2013","journal-title":"Proteomics"},{"key":"10.1016\/j.psj.2026.106821_bib0022","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innov. (Camb)"},{"key":"10.1016\/j.psj.2026.106821_bib0023","article-title":"Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality","volume":"13","author":"Wu","year":"2022","journal-title":"Front. Genet."},{"key":"10.1016\/j.psj.2026.106821_bib0024","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101098","article-title":"Prolonged scotophase within a 24 hour light regime improves eggshell quality by enhancing calcium deposition in laying hens","volume":"100","author":"Xin","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105111","article-title":"The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell","volume":"104","author":"Yang","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0027","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1186\/s12864-019-6017-2","article-title":"The differences of gonadal hormones and uterine transcriptome during shell calcification of hens laying hard or weak-shelled eggs","volume":"20","author":"Zhang","year":"2019","journal-title":"Bmc Genom."},{"key":"10.1016\/j.psj.2026.106821_bib0026","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105246","article-title":"Genetic markers associated with bone strength and density in Rhode Island Red laying hens","volume":"104","author":"Yue","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.106821_bib0028","first-page":"1396","article-title":"Multi-omics reveals key cell types and gene families regulating eggshell strength in chicken uteri","volume":"46","author":"Zhang","year":"2025","journal-title":"Zool. Res."},{"key":"10.1016\/j.psj.2026.106821_bib0029","article-title":"Age-related changes in eggshell physical properties, ultrastructure, calcium metabolism-related serum indices, and gene expression in eggshell gland during eggshell formation in commercial laying ducks","volume":"101","author":"Zhang","year":"2022","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126004505?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126004505?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T23:15:00Z","timestamp":1782602100000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126004505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S0032579126004505"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.106821","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Deciphering the uterine transcriptional mechanisms underlying eggshell strength through multi-stage analysis in laying hens","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.106821","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"106821"}}