{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:04:57Z","timestamp":1784689497220,"version":"3.55.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004835","name":"Zhejiang University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004835","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972630"],"award-info":[{"award-number":["31972630"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022314","name":"Center for Veterinary Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100022314","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.psj.2026.107120","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:27:29Z","timestamp":1778858849000},"page":"107120","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polysaccharide from Atractylodes macrocephala Koidz. protects against avian pathogenic Escherichia coli-induced intestinal barrier Dysfunction via Suppressing PI3K\/Akt-Mediated Claudin-2 upregulation"],"prefix":"10.1016","volume":"105","author":[{"given":"Shuijing","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiusheng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.107120_bib0001","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1648736","article-title":"Baicalin-copper complex alleviates intestinal damage in avian pathogenic Escherichia coli-infected chicks by targeting the AKT\/NF-\u03baB pathway","volume":"12","author":"Cao","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0002","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105369","article-title":"Co-infection with Proteus mirabilis and extraintestinal pathogenic Escherichia coli aggravated liver injury in chickens by disrupting the jejunal barrier","volume":"104","author":"Cen","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2024.113014","article-title":"Polysaccharide of atractylodes macrocephala koidz alleviates NAFLD-induced hepatic inflammation in mice by modulating the TLR4\/MyD88\/NF-\u03bab pathway","volume":"141","author":"Chen","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.psj.2026.107120_bib0004","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/s41385-018-0118-0","article-title":"IL-10 signaling prevents gluten-dependent intraepithelial CD4+ cytotoxic T lymphocyte infiltration and epithelial damage in the small intestine","volume":"12","author":"Costes","year":"2019","journal-title":"Mucosal Immunol."},{"key":"10.1016\/j.psj.2026.107120_bib0005","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1080\/15548627.2021.2016233","article-title":"AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability","volume":"18","author":"Ganapathy","year":"2022","journal-title":"Autophagy"},{"key":"10.1016\/j.psj.2026.107120_bib0006","doi-asserted-by":"crossref","first-page":"464","DOI":"10.4103\/ijmr.IJMR_3514_20","article-title":"Role of alternatives to antibiotics in mitigating the antimicrobial resistance crisis","volume":"156","author":"Gupta","year":"2022","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.psj.2026.107120_bib0007","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/s41575-022-00675-x","article-title":"The role of goblet cells and mucus in intestinal homeostasis","volume":"19","author":"Gustafsson","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.psj.2026.107120_bib0008","doi-asserted-by":"crossref","first-page":"28225","DOI":"10.1021\/acs.jafc.4c08816","article-title":"Plant-derived polysaccharides benefit weaned piglets by regulating intestinal microbiota: a review","volume":"72","author":"Huang","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.psj.2026.107120_bib0009","article-title":"Cynaroside ameliorates TNBS-induced colitis by inhibiting intestinal epithelial cell apoptosis via the PI3K\/AKT signalling pathway","volume":"15","author":"Huang","year":"2024","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.psj.2026.107120_bib0010","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1016\/j.bbamem.2015.07.003","article-title":"Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells","volume":"1848","author":"Ikari","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.psj.2026.107120_bib0011","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.148855","article-title":"Polysaccharides of atractylodes macrocephala koidz effectively improve the EpCAM\u00b1 genotype-related inflammatory bowel diseases via modulating gut microbiota","volume":"333","author":"Kong","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.psj.2026.107120_bib0012","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/j.1749-6632.2012.06555.x","article-title":"Charge-selective claudin channels","volume":"1257","author":"Krug","year":"2012","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0014","article-title":"PAMK relieves LPS-induced enteritis and improves intestinal flora disorder in Goslings. Evid.based complement","volume":"2021","author":"Li","year":"2021","journal-title":"Altern. Med. ECAM"},{"key":"10.1016\/j.psj.2026.107120_bib0015","series-title":"Master Thesis","article-title":"The preventive effect of the fermented pulsatilla compound by bacillus subtilis on broiler chickens infected with escherichia coli","author":"Lu","year":"2023"},{"key":"10.1016\/j.psj.2026.107120_bib0016","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105160","article-title":"Protective effects of polysaccharide of atractylodes macrocephala Koidz and Jiawei Si-jun-zi decoction on gut health and immune function in cyclophosphamide-treated chicks","volume":"104","author":"Lu","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0017","doi-asserted-by":"crossref","DOI":"10.4161\/21688370.2014.977176","article-title":"Claudin-2 as a mediator of leaky gut barrier during intestinal inflammation","volume":"3","author":"Luettig","year":"2015","journal-title":"Tissue Barrier."},{"key":"10.1016\/j.psj.2026.107120_bib0018","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1002\/mnfr.201600274","article-title":"The ginger component 6-shogaol prevents TNF-\u03b1-induced barrier loss via inhibition of PI3K\/akt and NF-\u03bab signaling","volume":"60","author":"Luettig","year":"2016","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.psj.2026.107120_bib0019","first-page":"4103","article-title":"Advances on the experimental infectious models of avian pathologenic Escherichia coli","volume":"47","author":"Ma","year":"2020","journal-title":"China Anim. Husb. Vet. Med."},{"key":"10.1016\/j.psj.2026.107120_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104359","article-title":"Avian pathogenic Escherichia coli (APEC): current insights and future challenges","volume":"103","author":"Nawaz","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0021","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2217877121","article-title":"Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis","volume":"121","author":"Oami","year":"2024","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.psj.2026.107120_bib0022","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1017\/S146625232300004X","article-title":"Alternatives to antibiotics in veterinary medicine: considerations for the management of Johne\u2019s disease","volume":"24","author":"O\u2019Connell","year":"2023","journal-title":"Anim. Health Res. Rev."},{"key":"10.1016\/j.psj.2026.107120_bib0023","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2022.115578","article-title":"Bidirectional effects and mechanisms of traditional Chinese medicine","volume":"298","author":"Peng","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.psj.2026.107120_bib0024","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1111\/jpn.12644","article-title":"Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R + IPEC-J2 cell through the PI3 k\/akt\/mTOR\/p70S6K signalling pathway","volume":"101","author":"Qi","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"10.1016\/j.psj.2026.107120_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.146197","article-title":"Plant polysaccharides mitigate ionizing radiation injury via multi-tissue protection and multi-target mechanisms: a review","volume":"320","author":"Ruiqing","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.psj.2026.107120_bib0026","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2024.121660","article-title":"Combined exposure of emamectin benzoate and microplastics induces tight junction disorder, immune disorder and inflammation in carp midgut via lysosome\/ROS\/ferroptosis pathway","volume":"257","author":"Shi","year":"2024","journal-title":"Water Res."},{"key":"10.1016\/j.psj.2026.107120_bib0027","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1016\/S0140-6736(24)01076-6","article-title":"Antimicrobial resistance: an agenda for all","volume":"403","year":"2024","journal-title":"Lancet"},{"key":"10.1016\/j.psj.2026.107120_bib0028","series-title":"Master Thesis","article-title":"Effect of astragalus polysaccharide on chick bacterial enteritis","author":"Tian","year":"2021"},{"key":"10.1016\/j.psj.2026.107120_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.106049","article-title":"Analysis of avian pathogenic Escherichia Coli (APEC) and its antimicrobial resistance risk characteristics and critical control points in laying hens","volume":"104","author":"Tian","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0030","doi-asserted-by":"crossref","first-page":"5655","DOI":"10.3390\/ijms20225655","article-title":"Claudin-2: roles beyond permeability functions","volume":"20","author":"Venugopal","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0031","series-title":"Master Thesis","article-title":"Application of pomegranate peel on growth performance of broilers and its resistance to chickens with colibacillosis","author":"Wang","year":"2023"},{"key":"10.1016\/j.psj.2026.107120_bib0032","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1002\/jcb.23067","article-title":"Non-proteolytic house dust mite allergen, der p 2, upregulated expression of tight junction molecule claudin-2 associated with Akt\/GSK-3\u03b2\/\u03b2-catenin signaling pathway","volume":"112","author":"Wang","year":"2011","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.psj.2026.107120_bib0033","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105005","article-title":"Chlorogenic acid mitigates avian pathogenic Escherichia coli-induced intestinal barrier damage in broiler chickens via anti-inflammatory and antioxidant effects","volume":"104","author":"Wei","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0034","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2020.529657","article-title":"Polysaccharide of atractylodes macrocephala koidz (PAMK) alleviates cyclophosphamide-induced immunosuppression in mice by upregulating CD28\/IP3R\/plc\u03b3-1\/AP-1\/NFAT signal pathway","volume":"11","author":"Xiang","year":"2020","journal-title":"Front Pharmacol."},{"key":"10.1016\/j.psj.2026.107120_bib0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2021.118821","article-title":"The potential roles of natural plant polysaccharides in inflammatory bowel disease: a review","volume":"277","author":"Yang","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.psj.2026.107120_bib0036","doi-asserted-by":"crossref","first-page":"8346","DOI":"10.1002\/jsfa.14417","article-title":"Protective effects of plant polysaccharides on intestinal health via targeted regulation of gut microbiota","volume":"105","author":"Ye","year":"2025","journal-title":"J. Sci. Food Agric."},{"key":"10.1016\/j.psj.2026.107120_bib0037","doi-asserted-by":"crossref","first-page":"731","DOI":"10.5713\/ajas.15.0415","article-title":"The effects of glucagon-like peptide-2 on the tight junction and barrier function in IPEC-J2 cells through phosphatidylinositol 3-kinase-protein kinase B-mammalian target of rapamycin signaling pathway","volume":"29","author":"Yu","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0038","doi-asserted-by":"crossref","DOI":"10.1093\/jas\/skaf327","article-title":"Zinc-loaded montmorillonite alleviates avian pathogenic Escherichia coli-induced intestinal barrier damage in broiler chickens","volume":"103","author":"Zha","year":"2025","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.psj.2026.107120_bib0039","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1186\/s12967-024-05168-w","article-title":"Blockade of PI3K\/AKT signaling pathway by Astragaloside IV attenuates ulcerative colitis via improving the intestinal epithelial barrier","volume":"22","author":"Zhang","year":"2024","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.psj.2026.107120_bib0040","series-title":"The Intervention Effect and Mechanism of Theaflavin-3\u2019-gallate on ETEC Infected IPEC-J2 Cells","author":"Zhao","year":"2023"},{"key":"10.1016\/j.psj.2026.107120_bib0041","article-title":"Gegen Qinlian decoction maintains colonic mucosal homeostasis in acute\/chronic ulcerative colitis via bidirectionally modulating dysregulated Notch signaling","volume":"68","author":"Zhao","year":"2020","journal-title":"Phytomedicine Int. J. Phytother. Phytopharm."},{"key":"10.1016\/j.psj.2026.107120_bib0042","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2024.1402465","article-title":"Bacteroides fragilis capsular polysaccharide A ameliorates ulcerative colitis in rat by recovering intestinal barrier integrity and restoring gut microbiota","volume":"15","author":"Zhong","year":"2024","journal-title":"Front. Pharmacol."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126007492?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126007492?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:43:17Z","timestamp":1784688197000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126007492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["S0032579126007492"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.107120","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Polysaccharide from Atractylodes macrocephala Koidz. protects against avian pathogenic Escherichia coli-induced intestinal barrier Dysfunction via Suppressing PI3K\/Akt-Mediated Claudin-2 upregulation","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.107120","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"107120"}}