{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:21:55Z","timestamp":1793276515142,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2000804"],"award-info":[{"award-number":["2023YFD2000804"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172688"],"award-info":[{"award-number":["32172688"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.psj.2026.107182","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T23:43:45Z","timestamp":1779752625000},"page":"107182","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Influence of age on eggshell quality in ducks: An analysis based on calcium metabolism, gut microbiota, and uterine transcriptome"],"prefix":"10.1016","volume":"105","author":[{"given":"Xihuai","family":"Xiong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6271-6184","authenticated-orcid":false,"given":"Fangxiong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6434-553X","authenticated-orcid":false,"given":"Yansen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.psj.2026.107182_bib0001","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.3382\/ps.0731590","article-title":"Duodenal calcium uptake, femur ash, and eggshell quality decline with age and increase following Molt1","volume":"73","author":"Al-batshan","year":"1994","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0002","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1093\/jn\/117.10.1775","article-title":"Vitamin D metabolism and calbindin (calcium-binding protein) in aged laying hens","volume":"117","author":"Bar","year":"1987","journal-title":"J. Nutr."},{"key":"10.1016\/j.psj.2026.107182_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101287","article-title":"Research note: changes in eggshell quality and microstructure related to hen age during a production cycle","volume":"100","author":"Benavides-Reyes","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0004","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics."},{"key":"10.1016\/j.psj.2026.107182_bib0005","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijfoodmicro.2010.06.029","article-title":"Recovery of Salmonella and Escherichia coli from commercial egg shells and effect of translucency on bacterial penetration in eggs","volume":"142","author":"Chousalkar","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.psj.2026.107182_bib0006","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.102008","article-title":"Intestinal microbiota of layer hens and its association with egg quality and safety","volume":"101","author":"Dai","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0007","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.3390\/foods12061340","article-title":"Non-destructive eggshell strength assessment using Hertz Contact theory\u2014part II: implementation and validation","volume":"12","author":"De Ketelaere","year":"2023","journal-title":"Foods."},{"key":"10.1016\/j.psj.2026.107182_bib0008","author":"Deng"},{"key":"10.1016\/j.psj.2026.107182_bib0009","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics."},{"key":"10.1016\/j.psj.2026.107182_bib0010","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.theriogenology.2023.04.007","article-title":"Aging-associated increased nitric oxide production is a potential cause of inferior eggshell quality produced by aged laying hens","volume":"205","author":"Elhamouly","year":"2023","journal-title":"Theriogenology"},{"key":"10.1016\/j.psj.2026.107182_bib0011","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1038\/nm.4385","article-title":"Targeting cellular senescence prevents age-related bone loss in mice","volume":"23","author":"Farr","year":"2017","journal-title":"Nat. Med."},{"key":"10.1016\/j.psj.2026.107182_bib0012","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1186\/s12864-020-07177-7","article-title":"Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens","volume":"21","author":"Feng","year":"2020","journal-title":"BMC. Genomics."},{"key":"10.1016\/j.psj.2026.107182_bib0013","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104661","article-title":"TMT-based quantitative proteomic analysis reveals age-related changes in eggshell matrix proteins and their correlation with eggshell quality in Xinyang blue-shelled laying hens","volume":"104","author":"Fu","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0014","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40104-023-00986-2","article-title":"Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens","volume":"15","author":"Fu","year":"2024","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.1016\/j.psj.2026.107182_bib0015","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.aninu.2024.04.026","article-title":"Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders","volume":"19","author":"Gao","year":"2024","journal-title":"Anim. Nutr."},{"key":"10.1016\/j.psj.2026.107182_bib0016","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12860-021-00350-0","article-title":"Avian eggshell biomineralization: an update on its structure, mineralogy and protein tool kit","volume":"22","author":"Gautron","year":"2021","journal-title":"BMC. Mol. Cell Biol."},{"key":"10.1016\/j.psj.2026.107182_bib0017","article-title":"Gene cloning of S100\u03b2 and NGF and localization of their expression in the small intestine of broilers of various ages","volume":"15","author":"Han","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.psj.2026.107182_bib0018","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1089\/cmb.2017.0096","article-title":"cutPrimers: a new tool for accurate cutting of primers from reads of targeted next generation sequencing","volume":"24","author":"Kechin","year":"2017","journal-title":"J. Comput. Biol."},{"key":"10.1016\/j.psj.2026.107182_bib0019","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1017\/S0043933912000086","article-title":"Concepts and methods for understanding bone metabolism in laying hens","volume":"68","author":"Kim","year":"2012","journal-title":"World\u2019s Poultry Sci. Journal"},{"key":"10.1016\/j.psj.2026.107182_bib0020","author":"Li"},{"key":"10.1016\/j.psj.2026.107182_bib0021","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics."},{"key":"10.1016\/j.psj.2026.107182_bib0022","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.5713\/ab.22.0003","article-title":"Interactive effect of dietary levels of calcium and 25-hydroxy vitamin D3 on the performance, serum biochemical concentration and digestibility of laying hens from 61 to 70 weeks of age","volume":"35","author":"Lim","year":"2022","journal-title":"Anim. Biosci."},{"key":"10.1016\/j.psj.2026.107182_bib0023","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.3382\/ps\/pew222","article-title":"Genetic parameters of body weight, egg production, and shell quality traits in the Shan Ma laying duck (Anas platyrhynchos)","volume":"95","author":"Lin","year":"2016","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0024","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.crpv.2004.08.002","article-title":"Avian eggshell mineralization: biochemical and functional characterization of matrix proteins","volume":"3","author":"Nys","year":"2004","journal-title":"Comptes Rendus Palevol"},{"key":"10.1016\/j.psj.2026.107182_bib0025","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-99566-5","article-title":"Jejunal transcriptomic profiling of two layer strains throughout the entire production period","volume":"11","author":"Omotoso","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.psj.2026.107182_bib0026","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.5851\/kosfa.2018.e41","article-title":"The influence of hen aging on eggshell ultrastructure and shell mineral components","volume":"38","author":"Park","year":"2018","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"10.1016\/j.psj.2026.107182_bib0027","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.gendis.2019.12.005","article-title":"Microbiome data analysis with applications to pre-clinical studies using QIIME2: statistical considerations","volume":"8","author":"Rai","year":"2021","journal-title":"Genes. Dis."},{"key":"10.1016\/j.psj.2026.107182_bib0028","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics."},{"key":"10.1016\/j.psj.2026.107182_bib0029","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-35203-y","article-title":"RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization","volume":"8","author":"Sah","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.psj.2026.107182_bib0030","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2023.1112499","article-title":"Physiological regulation of calcium and phosphorus utilization in laying hens","volume":"14","author":"Sinclair-Black","year":"2023","journal-title":"Front. Physiol."},{"key":"10.1016\/j.psj.2026.107182_bib0031","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105558","article-title":"Investigating the correlation between phenotype, cecal microbiome, and serum metabolome in laying ducks with different shell strength","volume":"104","author":"Sun","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0032","series-title":"Improving the Safety and Quality of Eggs and Egg Products.","first-page":"300","article-title":"Effect of hen age, moult, laying environment and egg storage on egg quality","author":"Travel","year":"2011"},{"key":"10.1016\/j.psj.2026.107182_bib0033","article-title":"Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production","volume":"100","author":"Wang","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0034","doi-asserted-by":"crossref","first-page":"575","DOI":"10.3390\/metabo11090575","article-title":"Uterine metabolomic analysis for the regulation of eggshell calcification in chickens","volume":"11","author":"Wang","year":"2021","journal-title":"Metabolites."},{"key":"10.1016\/j.psj.2026.107182_bib0035","doi-asserted-by":"crossref","DOI":"10.1186\/s40793-020-00358-7","article-title":"Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences","volume":"15","author":"Wemheuer","year":"2020","journal-title":"Environ. Microbiome"},{"key":"10.1016\/j.psj.2026.107182_bib0036","doi-asserted-by":"crossref","first-page":"3086","DOI":"10.3390\/ani15213086","article-title":"Improved eggshell quality in aged hens through circadian gut microbiota and metabolite changes induced by a 28-h ahemeral light cycle","volume":"15","author":"Xu","year":"2025","journal-title":"Animals"},{"key":"10.1016\/j.psj.2026.107182_bib0037","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s11033-014-3772-7","article-title":"A novel SMAD family protein, SMAD9 is involved in follicular initiation and changes egg yield of geese via synonymous mutations in exon1 and intron2","volume":"42","author":"Xu","year":"2015","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.psj.2026.107182_bib0038","article-title":"Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage","volume":"14","author":"Xu","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.psj.2026.107182_bib0039","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2025.105111","article-title":"The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell","volume":"104","author":"Yang","year":"2025","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0040","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.theriogenology.2018.11.022","article-title":"A potential role for SMAD9 in goose follicular selection through regulation of mRNA levels of luteinizing hormone receptor","volume":"135","author":"Yu","year":"2019","journal-title":"Theriogenology"},{"key":"10.1016\/j.psj.2026.107182_bib0041","article-title":"Age-related changes in eggshell physical properties, ultrastructure, calcium metabolism-related serum indices, and gene expression in eggshell gland during eggshell formation in commercial laying ducks","volume":"101","author":"Zhang","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0042","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.103832","article-title":"Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks","volume":"103","author":"Zhu","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.psj.2026.107182_bib0043","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.5713\/ajas.16.0470","article-title":"Ovarian transcriptomic analysis of Shan Ma ducks at peak and late stages of egg production","volume":"30","author":"Zhu","year":"2017","journal-title":"Asian-Australas. J. Anim. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126008138?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579126008138?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T11:43:36Z","timestamp":1787571816000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579126008138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S0032579126008138"],"URL":"https:\/\/doi.org\/10.1016\/j.psj.2026.107182","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Influence of age on eggshell quality in ducks: An analysis based on calcium metabolism, gut microbiota, and uterine transcriptome","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.psj.2026.107182","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Poultry Science Association Inc.","name":"copyright","label":"Copyright"}],"article-number":"107182"}}