{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:58:44Z","timestamp":1791129524533,"version":"4.1.0"},"reference-count":23,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trends in Parasitology"],"published-print":{"date-parts":[[2007,8]]},"DOI":"10.1016\/j.pt.2007.06.002","type":"journal-article","created":{"date-parts":[[2007,6,20]],"date-time":"2007-06-20T11:19:19Z","timestamp":1182338359000},"page":"344-347","source":"Crossref","is-referenced-by-count":47,"title":["Cryptosporidium oocysts: challenging adversaries?"],"prefix":"10.1016","volume":"23","author":[{"given":"Lucy J.","family":"Robertson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f8rn K.","family":"Gjerde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pt.2007.06.002_bib1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1017\/S0031182006001491","article-title":"Critical processes affecting Cryptosporidium oocyst survival in the environment","volume":"134","author":"King","year":"2007","journal-title":"Parasitology"},{"key":"10.1016\/j.pt.2007.06.002_bib2","unstructured":"Jenkins, M.B. et al. (1998) Authors\u2019 Reply to Letter to the Editor, Viability of Cryptosporidium parvum oocysts: assessment by the dye permeability assay, from L.J. Robertson et al. Appl. Environ. Microbiol. 64, 3544\u20133545"},{"key":"10.1016\/j.pt.2007.06.002_bib3","series-title":"Cryptosporidium: from Molecules to Disease","first-page":"3","article-title":"Cryptosporidium: they probably taste like chicken","author":"Upton","year":"2003"},{"key":"10.1016\/j.pt.2007.06.002_bib4","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.pt.2006.07.004","article-title":"The coccidian oocyst: a tough nut to crack!","volume":"22","author":"Belli","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.pt.2007.06.002_bib5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1017\/S0031182096008761","article-title":"Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual parasite development","volume":"114","author":"Spano","year":"1997","journal-title":"Parasitology"},{"key":"10.1016\/j.pt.2007.06.002_bib6","series-title":"Climate Change 2001: The Scientific Basis","author":"IPCC","year":"2001"},{"key":"10.1016\/j.pt.2007.06.002_bib7","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1098\/rspb.2004.3043","article-title":"Pathogen adaption to seasonal forcing and climate change","volume":"272","author":"Koelle","year":"2005","journal-title":"Proc. R. Soc. Lond. B. Biol. Sci."},{"key":"10.1016\/j.pt.2007.06.002_bib8","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.1128\/AEM.68.8.3809-3817.2002","article-title":"Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum","volume":"68","author":"Rochelle","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib9","unstructured":"Rochelle, P.A. et al. (2005) Comparing Cell Culture and Mouse Assays for Measuring Infectivity of Cryptosporidium. AwwaRF Report 91027F, IWA Publishing"},{"key":"10.1016\/j.pt.2007.06.002_bib10","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1128\/AEM.71.3.1495-1500.2005","article-title":"Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection","volume":"71","author":"Di Giovanni","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib11","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1128\/AEM.62.9.3385-3390.1996","article-title":"Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR","volume":"62","author":"Stinear","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib12","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1128\/AEM.65.4.1584-1588.1999","article-title":"beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability","volume":"65","author":"Widmer","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib13","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0167-7012(00)00198-6","article-title":"Estimating viability of Cryptosporidium parvum oocysts using reverse transcriptase-polymerase chain reaction (RT-PCR) directed at mRNA encoding amyloglucosidase","volume":"43","author":"Jenkins","year":"2000","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.pt.2007.06.002_bib14","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1046\/j.1462-2920.2003.00442.x","article-title":"An immunomagnetic separation\u2013reverse transcription polymerase chain reaction (IMS-RT-PCR) test for sensitive and rapid detection of viable waterborne Cryptosporidium parvum","volume":"5","author":"Hallier-Soulier","year":"2003","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib15","first-page":"1","article-title":"Comparison of tests for viable and infectious Cryptosporidium parvum oocysts","volume":"89","author":"Jenkins","year":"2003","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.pt.2007.06.002_bib16","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1046\/j.1365-2672.1998.853496.x","article-title":"The use of a ribosomal RNA targeted oligonucleotide probe for fluorescent labelling of viable Cryptosporidium parvum oocysts","volume":"85","author":"Vesey","year":"1998","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib17","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1365-2672.2004.02459.x","article-title":"Environmental inactivation of Cryptosporidium oocysts in catchment soils","volume":"98","author":"Davies","year":"2005","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib18","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0378-1097(03)00538-X","article-title":"Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts","volume":"226","author":"Fontaine","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.pt.2007.06.002_bib19","unstructured":"McClellan, P. (1998) Cryptosporidium and Giardia: a picture of uncertainty (Chapter 8). In: Sydney Water Inquiry (Fifth Report, Final Report Volume 2), New South Wales Premier's Department (http:\/\/www.premiers.nsw.gov.au\/our_library\/archives\/sydwater\/5threp\/r5chapter8.html)"},{"key":"10.1016\/j.pt.2007.06.002_bib20","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s00436-006-0276-y","article-title":"Fluorescent in situ hybridization as a tool to retrospectively identify Cryptosporidium parvum and Giardia lamblia in samples from terrestrial mammalian wildlife","volume":"100","author":"Bednarska","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.pt.2007.06.002_bib21","doi-asserted-by":"crossref","first-page":"4514","DOI":"10.1128\/AEM.61.12.4514-4516.1995","article-title":"Detection of viable Cryptosporidium parvum oocysts by PCR","volume":"61","author":"Wagner-Wiening","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pt.2007.06.002_bib22","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S0020-7519(97)00033-7","article-title":"Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability","volume":"27","author":"Belosevic","year":"1997","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.pt.2007.06.002_bib23","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/AEM.58.11.3488-3493.1992","article-title":"Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes","volume":"58","author":"Campbell","year":"1992","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Trends in Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1471492207001419?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1471492207001419?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T05:46:24Z","timestamp":1587620784000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1471492207001419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["S1471492207001419"],"URL":"https:\/\/doi.org\/10.1016\/j.pt.2007.06.002","relation":{},"ISSN":["1471-4922"],"issn-type":[{"value":"1471-4922","type":"print"}],"subject":[],"published":{"date-parts":[[2007,8]]}}}