{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T21:11:05Z","timestamp":1718313065593},"reference-count":41,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,11,21]],"date-time":"2015-11-21T00:00:00Z","timestamp":1448064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014250","name":"Fondo Tecnol\u00f3gico Argentino","doi-asserted-by":"crossref","award":["ANR600 NA 218\/08"],"award-info":[{"award-number":["ANR600 NA 218\/08"]}],"id":[{"id":"10.13039\/501100014250","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.es","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Revista Argentina de Microbiolog\u00eda"],"published-print":{"date-parts":[[2016,1]]},"DOI":"10.1016\/j.ram.2015.10.004","type":"journal-article","created":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T06:06:57Z","timestamp":1452838017000},"page":"5-14","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding"],"prefix":"10.1016","volume":"48","author":[{"given":"Mar\u00eda Elisa","family":"Pavan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban Enrique","family":"Pavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabi\u00e1n Mart\u00edn","family":"Cair\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Julia","family":"Pettinari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ram.2015.10.004_bib0210","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1006\/bbrc.2001.5337","article-title":"Rapid purification of recombinant anthrax-protective antigen under nondenaturing conditions","volume":"286","author":"Ahuja","year":"2001","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.ram.2015.10.004_bib0215","doi-asserted-by":"crossref","first-page":"9375","DOI":"10.1039\/c3cp50207d","article-title":"Reversibility in protein folding: effect of \u03b2-cyclodextrin on bovine serum albumin unfolded by sodium dodecyl sulphate","volume":"15","author":"Anand","year":"2013","journal-title":"Phys Chem Chem Phys"},{"key":"10.1016\/j.ram.2015.10.004_bib0220","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1128\/CVI.05556-11","article-title":"Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracis","volume":"19","author":"Bellanti","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.ram.2015.10.004_bib0225","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.ram.2015.10.004_bib0230","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.pep.2009.02.004","article-title":"Cooperative effects of urea and l-arginine on protein refolding","volume":"66","author":"Chen","year":"2009","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0235","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.phrp.2012.07.006","article-title":"Serological correlate of protection in guinea pigs for a recombinant protective antigen anthrax vaccine produced from Bacillus brevis","volume":"3","author":"Chun","year":"2012","journal-title":"Osong Public Health Res Perspect"},{"key":"10.1016\/j.ram.2015.10.004_bib0240","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.mam.2009.08.006","article-title":"Anthrax vaccination strategies","volume":"30","author":"Cybulski","year":"2009","journal-title":"Mol Aspects Med"},{"key":"10.1016\/j.ram.2015.10.004_bib0245","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.ab.2008.11.016","article-title":"A nondetergent sulfobetaine improves protein unfolding reversibility in microcalorimetric studies","volume":"385","author":"D\u2019Amico","year":"2009","journal-title":"Anal Biochem"},{"key":"10.1016\/j.ram.2015.10.004_bib0250","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.pep.2010.09.008","article-title":"A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions","volume":"75","author":"Dechavanne","year":"2011","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0255","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.biologicals.2012.10.002","article-title":"Ability of ELISA and a toxin neutralization assay to detect changes in immunogenicity of a recombinant Bacillus anthracis protective antigen vaccine upon storage","volume":"41","author":"Dom\u00ednguez-Castillo","year":"2013","journal-title":"Biologicals"},{"key":"10.1016\/j.ram.2015.10.004_bib0260","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0301-4622(02)00299-5","article-title":"Physical-chemical features of non-detergent sulfobetaines active as protein-folding helpers","volume":"100","author":"Expert-Bezan\u00e7on","year":"2003","journal-title":"Biophys Chem"},{"key":"10.1016\/j.ram.2015.10.004_bib0265","series-title":"The proteomics protocols handbook","first-page":"571","article-title":"Protein identification and analysis tools on the ExPASy server","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.ram.2015.10.004_bib0270","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2174\/187220812799789172","article-title":"Non-chromatographic strategies for protein refolding","volume":"6","author":"Gautam","year":"2012","journal-title":"Recent Pat Biotechnol"},{"key":"10.1016\/j.ram.2015.10.004_bib0275","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/1348-0421.12014","article-title":"A field usable qualitative anti-protective antigen enzyme-linked immunosorbent assay for serodiagnosis of human anthrax","volume":"57","author":"Ghosh","year":"2013","journal-title":"Microbiol Immunol"},{"key":"10.1016\/j.ram.2015.10.004_bib0280","first-page":"394","article-title":"Immunity to anthrax: protective antigen present in cell-free culture filtrates","volume":"27","author":"Gladstone","year":"1946","journal-title":"Br J Exp Pathol"},{"key":"10.1016\/j.ram.2015.10.004_bib0285","doi-asserted-by":"crossref","first-page":"721","DOI":"10.7589\/0090-3558-44.3.721","article-title":"Naturally acquired anthrax antibodies in a cheetah (Acinonyxjubatus) in Botswana","volume":"44","author":"Good","year":"2008","journal-title":"J Wildl Dis"},{"key":"10.1016\/j.ram.2015.10.004_bib0290","doi-asserted-by":"crossref","first-page":"3679","DOI":"10.1016\/j.vaccine.2007.01.056","article-title":"Monitoring of ELISA-reactive antibodies against anthrax protective antigen (PA), lethal factor (LF), and toxin-neutralising antibodies in serum of individuals vaccinated against anthrax with the PA-based UK anthrax vaccine","volume":"25","author":"Grunow","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.ram.2015.10.004_bib0295","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1006\/prep.1999.1066","article-title":"Expression and purification of the recombinant protective antigen of Bacillus anthracis","volume":"16","author":"Gupta","year":"1999","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0300","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.pep.2005.04.009","article-title":"Scalable purification of Bacillus anthracis protective antigen from Escherichia coli","volume":"45","author":"Gwinn","year":"2006","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0305","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.pep.2004.08.007","article-title":"Production and purification of Bacillus anthracis protective antigen from Escherichia coli","volume":"38","author":"Laird","year":"2004","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0310","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.1016\/j.vaccine.2005.12.028","article-title":"Duration of protection of rabbits after vaccination with Bacillus anthracis recombinant protective antigen vaccine","volume":"24","author":"Little","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.ram.2015.10.004_bib0315","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1042\/BA20070245","article-title":"High-level expression and single-step purification of recombinant Bacillus anthracis protective antigen from Escherichia coli","volume":"52","author":"Lu","year":"2009","journal-title":"Biotechnol Appl Biochem"},{"key":"10.1016\/j.ram.2015.10.004_bib0320","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.4161\/hv.25337","article-title":"Protective-antigen (PA) based anthrax vaccines confer protection against inhalation anthrax by precluding the establishment of a systemic infection","volume":"9","author":"Merkel","year":"2013","journal-title":"Hum Vaccin Immunother"},{"key":"10.1016\/j.ram.2015.10.004_bib0325","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ab.2010.02.017","article-title":"Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1","volume":"401","author":"Michalke","year":"2010","journal-title":"Anal Biochem"},{"key":"10.1016\/j.ram.2015.10.004_bib0330","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1186\/1746-6148-9-265","article-title":"Quantitative anti-PA IgG ELISA; assessment and comparability with the anthrax toxin neutralization assay in goats","volume":"9","author":"Ndumnego","year":"2013","journal-title":"BMC Vet Res"},{"key":"10.1016\/j.ram.2015.10.004_bib0335","first-page":"77","article-title":"Estudio molecular de cepas argentinas de Bacillus anthracis","volume":"39","author":"Pavan","year":"2007","journal-title":"Rev Argent Microbiol"},{"key":"10.1016\/j.ram.2015.10.004_bib0340","first-page":"294","article-title":"Bacillus anthracis: una mirada molecular a un pat\u00f3geno c\u00e9lebre","volume":"43","author":"Pavan","year":"2011","journal-title":"Rev Argent Microbiol"},{"key":"10.1016\/j.ram.2015.10.004_bib0345","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1042\/bj2520753","article-title":"Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography","volume":"252","author":"Quinn","year":"1988","journal-title":"Biochem J"},{"key":"10.1016\/j.ram.2015.10.004_bib0350","doi-asserted-by":"crossref","first-page":"8543","DOI":"10.1093\/nar\/17.21.8543","article-title":"A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA","volume":"17","author":"Rychlik","year":"1989","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.ram.2015.10.004_bib0355","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.mam.2009.06.004","article-title":"Dr. Jekyll and Mr Hyde: a short history of anthrax","volume":"30","author":"Schwartz","year":"2009","journal-title":"Mol Aspects Med"},{"key":"10.1016\/j.ram.2015.10.004_bib0360","doi-asserted-by":"crossref","first-page":"5374","DOI":"10.1016\/j.vaccine.2007.05.003","article-title":"Evaluation of immune response to orally administered Sterne strain 34F2 anthrax vaccine","volume":"25","author":"Shakya","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.ram.2015.10.004_bib0365","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1006\/prep.1996.0005","article-title":"Expression and purification of anthrax toxin protective antigen from Escherichia coli","volume":"7","author":"Sharma","year":"1996","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0370","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1006\/prep.2001.1416","article-title":"Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2","volume":"22","author":"Sijwali","year":"2001","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.ram.2015.10.004_bib0375","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1263\/jbb.99.303","article-title":"Solubilization and refolding of bacterial inclusion body proteins","volume":"99","author":"Singh","year":"2005","journal-title":"J Biosci Bioeng"},{"key":"10.1016\/j.ram.2015.10.004_bib0380","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4102\/ojvr.v75i2.6","article-title":"Naturally acquired antibodies to Bacillus anthracis protective antigen in vultures of southern Africa","volume":"75","author":"Turnbull","year":"2008","journal-title":"Onderstepoort J Vet Res"},{"key":"10.1016\/j.ram.2015.10.004_bib0385","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1110\/ps.04806004","article-title":"High-throughput automated refolding screening of inclusion bodies","volume":"13","author":"Vincentelli","year":"2004","journal-title":"Protein Sci"},{"key":"10.1016\/j.ram.2015.10.004_bib0390","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ymeth.2011.08.010","article-title":"High-throughput protein expression screening and purification in Escherichia coli","volume":"55","author":"Vincentelli","year":"2011","journal-title":"Methods"},{"key":"10.1016\/j.ram.2015.10.004_bib0395","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1110\/ps.051433205","article-title":"Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions","volume":"14","author":"Willis","year":"2005","journal-title":"Protein Sci"},{"key":"10.1016\/j.ram.2015.10.004_bib0400","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3390\/biom4010235","article-title":"Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies","volume":"4","author":"Yamaguchi","year":"2014","journal-title":"Biomolecules"},{"key":"10.1016\/j.ram.2015.10.004_bib0405","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/biot.201200025","article-title":"Protein refolding using chemical refolding additives","volume":"8","author":"Yamaguchi","year":"2013","journal-title":"Biotechnol J"},{"key":"10.1016\/j.ram.2015.10.004_bib0410","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/bmc.2800","article-title":"Refolding of detergent-denatured lysozyme using \u03b2-cyclodextrin-assisted ion exchange chromatography","volume":"27","author":"Zhang","year":"2013","journal-title":"Biomed Chromatogr"}],"container-title":["Revista Argentina de Microbiolog\u00eda"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0325754115001509?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0325754115001509?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T20:32:27Z","timestamp":1718310747000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0325754115001509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["S0325754115001509"],"URL":"https:\/\/doi.org\/10.1016\/j.ram.2015.10.004","relation":{},"ISSN":["0325-7541"],"issn-type":[{"value":"0325-7541","type":"print"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding","name":"articletitle","label":"Article Title"},{"value":"Revista Argentina de Microbiolog\u00eda","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ram.2015.10.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Asociaci\u00f3n Argentina de Microbiolog\u00eda. Published by Elsevier Espa\u00f1a S.L.","name":"copyright","label":"Copyright"}]}}