{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:17:05Z","timestamp":1783754225191,"version":"3.55.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 110-2636-B-006-009"],"award-info":[{"award-number":["NSTC 110-2636-B-006-009"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112-2636-B-006-008"],"award-info":[{"award-number":["NSTC 112-2636-B-006-008"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 111-2314-B-006-086-MY3"],"award-info":[{"award-number":["NSTC 111-2314-B-006-086-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 113-2636-B-006-005"],"award-info":[{"award-number":["NSTC 113-2636-B-006-005"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 111-2636-B-006-012"],"award-info":[{"award-number":["NSTC 111-2636-B-006-012"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 109-2636-B-006-006"],"award-info":[{"award-number":["NSTC 109-2636-B-006-006"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","rbmojournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive BioMedicine Online"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.rbmo.2025.105233","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T22:26:45Z","timestamp":1755988005000},"page":"105233","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Attributes of a large cohort of human seminal extracellular vesicles with varied sperm features"],"prefix":"10.1016","volume":"52","author":[{"given":"Yu-Sheng","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsing-Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Min","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu-Chiung","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chiao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung-Yen","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9967-5037","authenticated-orcid":false,"given":"Shih-Chieh","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.rbmo.2025.105233_bib0001","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1093\/humrep\/dey072","article-title":"Exosomal micrornas in seminal plasma are markers of the origin of azoospermia and can predict the presence of sperm in testicular tissue","volume":"33","author":"Barcel\u00f3","year":"2018","journal-title":"Hum Reprod"},{"key":"10.1016\/j.rbmo.2025.105233_bib0002","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1111\/andr.12199","article-title":"Total motile sperm count has a superior predictive value over the who 2010 cut-off values for the outcomes of intracytoplasmic sperm injection cycles","volume":"4","author":"Borges","year":"2016","journal-title":"Andrology"},{"key":"10.1016\/j.rbmo.2025.105233_bib0003","doi-asserted-by":"crossref","first-page":"7022","DOI":"10.3390\/ijms21197022","article-title":"Exosome composition and seminal plasma proteome: A promising source of biomarkers of male infertility","volume":"21","author":"Candenas","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.rbmo.2025.105233_bib0004","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1007\/978-3-030-67171-6_19","article-title":"The role of extracellular vesicles in sperm function and male fertility","author":"Foot","year":"2021","journal-title":"Subcell Biochem"},{"key":"10.1016\/j.rbmo.2025.105233_bib0005","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/andr.12496","article-title":"Human prostasomes from normozoospermic and non-normozoospermic men show a differential protein expression pattern","volume":"6","author":"Garcia-Rodriguez","year":"2018","journal-title":"Andrology"},{"key":"10.1016\/j.rbmo.2025.105233_bib0006","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1093\/humrep\/dev058","article-title":"Total motile sperm count: A better indicator for the severity of male factor infertility than the who sperm classification system","volume":"30","author":"Hamilton","year":"2015","journal-title":"Hum Reprod"},{"key":"10.1016\/j.rbmo.2025.105233_bib0007","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v4.28680","article-title":"Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy","volume":"4","author":"Hoog","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.rbmo.2025.105233_bib0008","doi-asserted-by":"crossref","first-page":"5377","DOI":"10.3390\/ijms21155377","article-title":"The role of the epididymis and the contribution of epididymosomes to mammalian reproduction","volume":"21","author":"James","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.rbmo.2025.105233_bib0009","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0015-0282(02)03059-5","article-title":"Gene-based screening for y chromosome deletions in taiwanese men presenting with spermatogenic failure","volume":"77","author":"Lin","year":"2002","journal-title":"Fertil Steril"},{"key":"10.1016\/j.rbmo.2025.105233_bib0010","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1093\/humrep\/dead015","article-title":"The emerging role of extracellular vesicles in the testis","volume":"38","author":"Ma","year":"2023","journal-title":"Hum Reprod"},{"key":"10.1016\/j.rbmo.2025.105233_bib0011","first-page":"182","article-title":"Extracellular vesicles: Roles in gamete maturation, fertilization and embryo implantation","volume":"22","author":"Machtinger","year":"2016","journal-title":"Hum. Reprod. Update."},{"key":"10.1016\/j.rbmo.2025.105233_bib0012","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1093\/humrep\/dez114","article-title":"Proteomic analysis reveals the negative modulator of sperm function glycodelin as over-represented in semen exosomes isolated from asthenozoospermic patients","volume":"34","author":"Murdica","year":"2019","journal-title":"Hum Reprod"},{"key":"10.1016\/j.rbmo.2025.105233_bib0013","doi-asserted-by":"crossref","DOI":"10.1016\/j.fertnstert.2019.01.030","article-title":"Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation","volume":"111","author":"Murdica","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.rbmo.2025.105233_bib0014","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1016\/j.fertnstert.2021.01.014","article-title":"Clarifying the relationship between total motile sperm counts and intrauterine insemination pregnancy rates","volume":"115","author":"Muthigi","year":"2021","journal-title":"Fertil Steril"},{"key":"10.1016\/j.rbmo.2025.105233_bib0015","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41580-020-0251-y","article-title":"Rna delivery by extracellular vesicles in mammalian cells and its applications","volume":"21","author":"O\u2019brien","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.rbmo.2025.105233_bib0016","unstructured":"Organization, W.H., 2021. Who laboratory manual for the examination and processing of human semen. Who laboratory manual for the examination and processing of human semen."},{"key":"10.1016\/j.rbmo.2025.105233_bib0017","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.2001595","article-title":"Ca2+ signaling tools acquired from prostasomes are required for progesterone-induced sperm motility","volume":"4","author":"Park","year":"2011","journal-title":"Sci. Signal."},{"key":"10.1016\/j.rbmo.2025.105233_bib0018","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24054818","article-title":"Seminal extracellular vesicles and their involvement in male (in)fertility: A systematic review","volume":"24","author":"Parra","year":"2023","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.rbmo.2025.105233_bib0019","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1002\/pros.20860","article-title":"Structural heterogeneity and protein composition of exosome-like vesicles (prostasomes) in human semen","volume":"69","author":"Poliakov","year":"2009","journal-title":"The Prostate"},{"key":"10.1016\/j.rbmo.2025.105233_bib0020","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.fertnstert.2016.07.1101","article-title":"Seminal fluid and fertility in women","volume":"106","author":"Robertson","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.rbmo.2025.105233_bib0021","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1093\/molehr\/6.10.883","article-title":"Prostasomes inhibit the nadph oxidase activity of human neutrophils","volume":"6","author":"Saez","year":"2000","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.rbmo.2025.105233_bib0022","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1152\/physrev.00013.2018","article-title":"The female response to seminal fluid","volume":"100","author":"Schjenken","year":"2020","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.rbmo.2025.105233_bib0023","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1097\/JU.0000000000001521","article-title":"Diagnosis and treatment of infertility in men: Aua\/asrm guideline part i","volume":"205","author":"Schlegel","year":"2021","journal-title":"J Urol"},{"key":"10.1016\/j.rbmo.2025.105233_bib0024","doi-asserted-by":"crossref","first-page":"e7","DOI":"10.1016\/j.fertnstert.2019.02.044","article-title":"Understanding the role of seminal fluid exosomes within the male reproductive tract","volume":"111","author":"Stewart","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.rbmo.2025.105233_bib0025","doi-asserted-by":"crossref","DOI":"10.1111\/aji.13338","article-title":"Roles of male reproductive tract extracellular vesicles in reproduction","volume":"85","author":"Tamessar","year":"2021","journal-title":"Am J Reprod Immunol"},{"key":"10.1016\/j.rbmo.2025.105233_bib0026","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.urology.2019.06.038","article-title":"Total motile sperm count trend over time: Evaluation of semen analyses from 119,972 men from subfertile couples","volume":"132","author":"Tiegs","year":"2019","journal-title":"Urology"},{"key":"10.1016\/j.rbmo.2025.105233_bib0027","doi-asserted-by":"crossref","DOI":"10.4161\/spmg.28218","article-title":"Review of azoospermia","author":"Wosnitzer","year":"2014","journal-title":"Spermatogenesis."},{"key":"10.1016\/j.rbmo.2025.105233_bib0028","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3390\/photonics11020101","article-title":"The advances and applications of characterization technique for exosomes: From dynamic light scattering to super-resolution imaging technology","volume":"11","author":"Wu","year":"2024","journal-title":"Photonics"},{"key":"10.1016\/j.rbmo.2025.105233_bib0029","doi-asserted-by":"crossref","first-page":"e423","DOI":"10.1002\/ctm2.423","article-title":"Quantitative proteomic biomarkers from extracellular vesicles of human seminal plasma in the differential diagnosis of azoospermia","volume":"11","author":"Yao","year":"2021","journal-title":"Clin Transl Med"}],"container-title":["Reproductive BioMedicine Online"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1472648325004407?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1472648325004407?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:08:35Z","timestamp":1783753715000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1472648325004407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S1472648325004407"],"URL":"https:\/\/doi.org\/10.1016\/j.rbmo.2025.105233","relation":{},"ISSN":["1472-6483"],"issn-type":[{"value":"1472-6483","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Attributes of a large cohort of human seminal extracellular vesicles with varied sperm features","name":"articletitle","label":"Article Title"},{"value":"Reproductive BioMedicine Online","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rbmo.2025.105233","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105233"}}