{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:29:45Z","timestamp":1778693385321,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Biology"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.repbio.2018.04.001","type":"journal-article","created":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T22:53:28Z","timestamp":1524696808000},"page":"177-181","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Sperm chromatin alterations in fertile and subfertile bulls"],"prefix":"10.1016","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4114-4655","authenticated-orcid":false,"given":"Elisson Ter\u00eancio","family":"Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Vieira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7690-301X","authenticated-orcid":false,"given":"Bruno Augusto Nassif","family":"Traven\u00e7olo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-8054","authenticated-orcid":false,"given":"Benner Geraldo","family":"Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Em\u00edlio","family":"Beletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.repbio.2018.04.001_bib0005","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/10520290410001700774","article-title":"A computational approach to the characterization of bovine sperm chromatin alterations","volume":"79","author":"Beletti","year":"2004","journal-title":"Biotech Histochem"},{"key":"10.1016\/j.repbio.2018.04.001_bib0010","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.theriogenology.2003.10.016","article-title":"Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology","volume":"62","author":"Beletti","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0015","first-page":"85","article-title":"Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa","volume":"22","author":"Beletti","year":"2005","journal-title":"Braz J Morphol Sci"},{"key":"10.1016\/j.repbio.2018.04.001_bib0020","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1098\/rstb.2009.0196","article-title":"Transcription and post-transcriptional regulation of spermatogenesis","volume":"365","author":"Bettegowda","year":"2010","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"10.1016\/j.repbio.2018.04.001_bib0025","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/molehr\/4.2.127","article-title":"In-situ competition between protamine and fluorochromes for sperm DNA","volume":"4","author":"Bizzaro","year":"1998","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.repbio.2018.04.001_bib0030","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.theriogenology.2017.11.006","article-title":"Effect of bovine sperm chromatin integrity evaluated using three different methods on in vitro fertility","volume":"107","author":"Castro","year":"2018","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0035","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/and.12250","article-title":"Localisation and quantification of alkali-labile sites in human spermatozoa by DNA breakage detection-fluorescence in situ hybridisation","volume":"47","author":"Cort\u00e9s-Guti\u00e9rrez","year":"2015","journal-title":"Andrologia"},{"key":"10.1016\/j.repbio.2018.04.001_bib0040","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1242\/bio.2012844","article-title":"Chromatin remodelling initiation during human spermiogenesis","volume":"1","author":"De Vries","year":"2012","journal-title":"Biol Open"},{"key":"10.1016\/j.repbio.2018.04.001_bib0045","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1095\/biolreprod.114.124255","article-title":"Sperm protamine-status correlates to the fertility of breeding bulls","volume":"92","author":"Dogan","year":"2015","journal-title":"Biol Reprod"},{"key":"10.1016\/j.repbio.2018.04.001_bib0050","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/j.1939-4640.2002.tb02599.x","article-title":"Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques","volume":"23","author":"Evenson","year":"2002","journal-title":"J Androl"},{"key":"10.1016\/j.repbio.2018.04.001_bib0055","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/j.1939-4640.2003.tb02641.x","article-title":"The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation","volume":"24","author":"Fern\u00e1ndez","year":"2003","journal-title":"J Androl"},{"key":"10.1016\/j.repbio.2018.04.001_bib0060","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.theriogenology.2004.09.023","article-title":"Sperm factors related to in vitro and in vivo porcine fertility","volume":"63","author":"Gadea","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0065","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/01485010117363","article-title":"Predictive value of sperm chromatin condensation (aniline blue staining) in the assessment of male fertility","volume":"46","author":"Hammadeh","year":"2001","journal-title":"Arch Androl"},{"key":"10.1016\/j.repbio.2018.04.001_bib0070","first-page":"125","article-title":"Connexin-43: A possible mediator of heat stress effects on ram Sertoli cells","volume":"6","author":"Hassanpour","year":"2015","journal-title":"Vet Res Forum"},{"key":"10.1016\/j.repbio.2018.04.001_bib0075","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.theriogenology.2016.08.017","article-title":"Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF","volume":"87","author":"Kipper","year":"2017","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0080","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.theriogenology.2016.03.015","article-title":"Selected sperm traits are simultaneously altered after scrotal heat stress and play specific roles in in vitro fertilization and embryonic development","volume":"86","author":"Lucio","year":"2016","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0085","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.theriogenology.2011.01.022","article-title":"Sperm morphometric subpopulations are differentially distributed in rams with different maturity age in cryopreserved ejaculates","volume":"76","author":"Mart\u00ed","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0090","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1530\/REP-09-0281","article-title":"packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics","volume":"139","author":"Miller","year":"2010","journal-title":"Reproduction"},{"key":"10.1016\/j.repbio.2018.04.001_bib0095","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.theriogenology.2012.02.009","article-title":"Restoration of seminal plasma to stallion spermatozoa selected by colloid centrifugation increases sperm progressive motility but is detrimental to chromatin integrity","volume":"78","author":"Morrell","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0100","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/j.theriogenology.2013.02.011","article-title":"Sperm chromatin structure and sperm morphology: their association with fertility in AI-dairy Ayrshire sires","volume":"79","author":"Nagy","year":"2013","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0105","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/humupd\/dml009","article-title":"Protamines and male infertility","volume":"12","author":"Oliva","year":"2006","journal-title":"Hum Reprod Update"},{"key":"10.1016\/j.repbio.2018.04.001_bib0110","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.anireprosci.2013.01.010","article-title":"Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program","volume":"137","author":"Oliveira","year":"2013","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.repbio.2018.04.001_bib0115","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/j.1939-4640.2001.tb02219.x","article-title":"Relationship of bull fertility to sperm nuclear shape","volume":"22","author":"Ostermeier","year":"2001","journal-title":"J Androl"},{"key":"10.1016\/j.repbio.2018.04.001_bib0120","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1095\/biolreprod.115.137646","article-title":"Absence of peroxiredoxin 6 amplifies the effect of oxidant stress on mobility and SCSA\/CMA3 defined chromatin quality and impairs fertilizing ability of mouse spermatozoa","volume":"94","author":"Ozkosem","year":"2016","journal-title":"Biol Reprod"},{"key":"10.1016\/j.repbio.2018.04.001_bib0125","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.theriogenology.2011.05.031","article-title":"Scrotal insulation and its relationship to abnormal morphology, chromatin protamination and nuclear shape of spermatozoa in Holstein-Friesian and Belgian Blue bulls","volume":"76","author":"Rahman","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0130","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1111\/j.2047-2927.2013.00111.x","article-title":"Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay","volume":"1","author":"Ribas-Maynou","year":"2013","journal-title":"Andrology"},{"key":"10.1016\/j.repbio.2018.04.001_bib0135","first-page":"148","article-title":"Semen evaluation techniques and their relationship with fertility","volume":"10","author":"Rodr\u00edguez-Mart\u00ednez","year":"2013","journal-title":"Anim Reprod"},{"key":"10.1016\/j.repbio.2018.04.001_bib0140","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1093\/humrep\/des471","article-title":"The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme","volume":"28","author":"Rogenhofer","year":"2013","journal-title":"Hum Reprod"},{"key":"10.1016\/j.repbio.2018.04.001_bib0145","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-1-62703-038-0_12","article-title":"fragmentation analysis using the TUNEL assay","volume":"927","author":"Sharma","year":"2013","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.repbio.2018.04.001_bib0150","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0015-0282(16)47963-X","article-title":"A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence","volume":"42","author":"Tejada","year":"1984","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2018.04.001_bib0155","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.fertnstert.2013.01.100","article-title":"Evaluation of sperm head shape at high magnification revealed correlation of sperm DNA fragmentation with aberrant head ellipticity and angularity","volume":"99","author":"Utsuno","year":"2013","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2018.04.001_bib0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2015.03.002","article-title":"Computer assisted sperm morphometry in mammals: a review","volume":"156","author":"Y\u00e1niz","year":"2015","journal-title":"Anim Reprod Sci"}],"container-title":["Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X17303479?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X17303479?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:18:22Z","timestamp":1761646702000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1642431X17303479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["S1642431X17303479"],"URL":"https:\/\/doi.org\/10.1016\/j.repbio.2018.04.001","relation":{},"ISSN":["1642-431X"],"issn-type":[{"value":"1642-431X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sperm chromatin alterations in fertile and subfertile bulls","name":"articletitle","label":"Article Title"},{"value":"Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.repbio.2018.04.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}