{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:37:53Z","timestamp":1777894673596,"version":"3.51.4"},"reference-count":42,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["31-1\/2017\/PDFSS-2017-18-RAJ-13366"],"award-info":[{"award-number":["31-1\/2017\/PDFSS-2017-18-RAJ-13366"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007229","name":"Harvard University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007229","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006919","name":"Massachusetts Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006919","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["RBMH\/Adhoc\/7\/2019-20"],"award-info":[{"award-number":["RBMH\/Adhoc\/7\/2019-20"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Biology"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.repbio.2021.100539","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T22:26:57Z","timestamp":1627424817000},"page":"100539","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Label-free proteomics of spermatozoa identifies candidate protein markers of idiopathic recurrent pregnancy loss"],"prefix":"10.1016","volume":"21","author":[{"given":"Sarla","family":"Naglot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil Kumar","family":"Tomar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neeta","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savita","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.repbio.2021.100539_bib0005","first-page":"247","article-title":"WHO: recommended definitions, terminology and format for statistical tables related to the perinatal period and use of a new certificate for cause of perinatal deaths. Modifications recommended by FIGO as amended October 14, 1976","volume":"56","author":"WHO","year":"1977","journal-title":"Acta Obstet Gynecol Scand"},{"key":"10.1016\/j.repbio.2021.100539_bib0010","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1097\/00003081-199409000-00016","article-title":"Recurrent miscarriage: epidemiologic factors, definitions, and incidence","volume":"37","author":"Hatasaka","year":"1994","journal-title":"Clin Obstet Gynecol"},{"key":"10.1016\/j.repbio.2021.100539_bib0015","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1055\/s-2000-13733","article-title":"Recurrent pregnancy loss: summary and clinical recommendations","volume":"18","author":"Lee","year":"2000","journal-title":"Semin Reprod Med"},{"key":"10.1016\/j.repbio.2021.100539_bib0020","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1097\/GRF.0b013e31802f1c28","article-title":"Evaluation and management of recurrent early pregnancy loss","volume":"50","author":"Stephenson","year":"2007","journal-title":"Clin Obstet Gynecol"},{"key":"10.1016\/j.repbio.2021.100539_bib0025","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1023\/A:1020315529090","article-title":"Implications of sperm chromosome abnormalities in recurrent miscarriage","volume":"16","author":"Rubio","year":"1999","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.repbio.2021.100539_bib0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000068533","article-title":"A novel family-specific translocation t(2;20)(p24.1;q13.1) associated with recurrent abortions: molecular characterization and segregation analysis in male meiosis","volume":"98","author":"Trappe","year":"2002","journal-title":"Cytogenet Genome Res"},{"key":"10.1016\/j.repbio.2021.100539_bib0035","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.fertnstert.2015.09.041","article-title":"Relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss","volume":"105","author":"Zidi-Jrah","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0040","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/S0015-0282(99)00495-1","article-title":"Embryonic karyotype of abortuses in relation to the number of previous miscarriages","volume":"73","author":"Ogasawara","year":"2000","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0045","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s10815-011-9647-0","article-title":"Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion","volume":"29","author":"Absalan","year":"2012","journal-title":"J Assist Reprod Genet"},{"key":"10.1016\/j.repbio.2021.100539_bib0050","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/humupd\/3.4.393","article-title":"The effect of male factors in repeated spontaneous abortion: lesson from in-vitro fertilization and intracytoplasmic sperm injection","volume":"3","author":"Hamamah","year":"1997","journal-title":"Hum Reprod Update"},{"key":"10.1016\/j.repbio.2021.100539_bib0055","doi-asserted-by":"crossref","first-page":"352","DOI":"10.3109\/19396368.2013.829536","article-title":"Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?","volume":"59","author":"Kiani-Esfahani","year":"2013","journal-title":"Syst Biol Reprod Med"},{"key":"10.1016\/j.repbio.2021.100539_bib0060","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3109\/19396368.2016.1148798","article-title":"Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: possible role in recurrent pregnancy loss","volume":"62","author":"Mohanty","year":"2016","journal-title":"Syst Biol Reprod Med"},{"key":"10.1016\/j.repbio.2021.100539_bib0065","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/mrd.90016","article-title":"Aberrant methylation patterns at the two-cell stage as an indicator of early developmental failure","volume":"63","author":"Shi","year":"2002","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.repbio.2021.100539_bib0070","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0015-0282(16)44528-0","article-title":"Genes, chromosomes, and reproductive failure","volume":"33","author":"Simpson","year":"1980","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0075","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/S0015-0282(98)00192-7","article-title":"Spermatozoa with chromosomal abnormalities may result in a higher rate of recurrent abortion","volume":"70","author":"Giorlandino","year":"1998","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0080","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1071\/R98030","article-title":"Increased chromosome abnormalities in human preimplantation embryos after in-vitro fertilization in patients with recurrent miscarriage","volume":"10","author":"Simon","year":"1998","journal-title":"Reprod Fertil Dev"},{"key":"10.1016\/j.repbio.2021.100539_bib0085","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/01485010290099390","article-title":"Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss","volume":"49","author":"Carrell","year":"2003","journal-title":"Arch Androl"},{"key":"10.1016\/j.repbio.2021.100539_bib0090","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2217\/bmm.10.100","article-title":"Differential proteomics of sperm: insights, challenges and future prospects","volume":"4","author":"Tomar","year":"2010","journal-title":"Biomark Med"},{"key":"10.1016\/j.repbio.2021.100539_bib0095","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.repbio.2021.100539_bib0100","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang da","year":"2009","journal-title":"Nat Protoc"},{"key":"10.1016\/j.repbio.2021.100539_bib0105","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gky1131","article-title":"STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.repbio.2021.100539_bib0110","doi-asserted-by":"crossref","first-page":"W214","DOI":"10.1093\/nar\/gkq537","article-title":"The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function","volume":"38","author":"Warde-Farley","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.repbio.2021.100539_bib0115","doi-asserted-by":"crossref","first-page":"7787","DOI":"10.1038\/s41598-020-64759-x","article-title":"Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways","volume":"10","author":"Saraswat","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.repbio.2021.100539_bib0120","doi-asserted-by":"crossref","first-page":"188","DOI":"10.3389\/fcell.2019.00188","article-title":"The role of sperm centrioles in human reproduction - the known and the unknown","volume":"7","author":"Avidor-Reiss","year":"2019","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.repbio.2021.100539_bib0125","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.urology.2011.05.049","article-title":"Semen parameters and sperm DNA fragmentation as causes of recurrent pregnancy loss","volume":"78","author":"Brahem","year":"2011","journal-title":"Urology"},{"key":"10.1016\/j.repbio.2021.100539_bib0130","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12610-018-0070-6","article-title":"Sperm aneuploidy and DNA fragmentation in unexplained recurrent pregnancy loss: a multicenter case-control study","volume":"28","author":"Esquerre-Lamare","year":"2018","journal-title":"Basic Clin Androl"},{"key":"10.1016\/j.repbio.2021.100539_bib0135","doi-asserted-by":"crossref","DOI":"10.21037\/tau.2017.06.15","article-title":"Infertility, recurrent pregnancy loss and sperm DNA fragmentation, have we found the missing link?","volume":"6","author":"Harlev","year":"2017","journal-title":"Transl Androl Urol"},{"key":"10.1016\/j.repbio.2021.100539_bib0140","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.repbio.2018.11.002","article-title":"The relationship between sperm DNA fragmentation, free radicals and antioxidant capacity with idiopathic repeated pregnancy loss","volume":"18","author":"Kamkar","year":"2018","journal-title":"Reprod Biol"},{"key":"10.1016\/j.repbio.2021.100539_bib0145","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.fertnstert.2015.10.033","article-title":"Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study","volume":"105","author":"Bareh","year":"2016","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0150","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.fertnstert.2019.03.003","article-title":"Sperm DNA fragmentation and recurrent pregnancy loss: a systematic review and meta-analysis","volume":"112","author":"McQueen","year":"2019","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2021.100539_bib0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.jogoh.2020.101740","article-title":"Sperm DNA fragmentation index with unexplained recurrent spontaneous abortion: a systematic review and meta-analysis","author":"Yifu","year":"2020","journal-title":"J Gynecol Obstet Hum Reprod"},{"key":"10.1016\/j.repbio.2021.100539_bib0160","first-page":"505","article-title":"Effects of varicocele repair on spontaneous first trimester miscarriage: a randomized clinical trial","volume":"9","author":"Mansour Ghanaie","year":"2012","journal-title":"Urol J"},{"key":"10.1016\/j.repbio.2021.100539_bib0165","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.rbmo.2012.05.002","article-title":"Relationship between varicocele and sperm DNA damage and the effect of varicocele repair: a meta-analysis","volume":"25","author":"Wang","year":"2012","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.repbio.2021.100539_bib0170","doi-asserted-by":"crossref","first-page":"103","DOI":"10.5534\/wjmh.190034","article-title":"Proteomic signatures in spermatozoa reveal the role of paternal factors in recurrent pregnancy loss","volume":"38","author":"Mohanty","year":"2020","journal-title":"World J Mens Health"},{"key":"10.1016\/j.repbio.2021.100539_bib0175","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s12958-019-0496-5","article-title":"Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss","volume":"17","author":"Xue","year":"2019","journal-title":"Reprod Biol Endocrinol"},{"key":"10.1016\/j.repbio.2021.100539_bib0180","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1095\/biolreprod59.5.1037","article-title":"Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa","volume":"59","author":"Aitken","year":"1998","journal-title":"Biol Reprod"},{"key":"10.1016\/j.repbio.2021.100539_bib0185","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s12272-015-0550-6","article-title":"ROS and energy metabolism in cancer cells: alliance for fast growth","volume":"38","author":"Kang","year":"2015","journal-title":"Arch Pharm Res"},{"key":"10.1016\/j.repbio.2021.100539_bib0190","first-page":"267","article-title":"Idiopathic recurrent pregnancy loss: role of paternal factors; a pilot study","volume":"12","author":"Imam","year":"2011","journal-title":"J Reprod Infertil"},{"key":"10.1016\/j.repbio.2021.100539_bib0195","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.lfs.2017.08.029","article-title":"Autophagy: the spotlight for cellular stress responses","volume":"188","author":"Ravanan","year":"2017","journal-title":"Life Sci"},{"key":"10.1016\/j.repbio.2021.100539_bib0200","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3389\/fnmol.2017.00170","article-title":"Autophagy and its impact on neurodegenerative diseases: new roles for TDP-43 and C9orf72","volume":"10","author":"Budini","year":"2017","journal-title":"Front Mol Neurosci"},{"key":"10.1016\/j.repbio.2021.100539_bib0205","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1093\/humrep\/deq098","article-title":"Y chromosome microdeletions, sperm DNA fragmentation and sperm oxidative stress as causes of recurrent spontaneous abortion of unknown etiology","volume":"25","author":"Bellver","year":"2010","journal-title":"Hum Reprod"},{"key":"10.1016\/j.repbio.2021.100539_bib0210","first-page":"550","article-title":"Sperm DNA damage & oxidative stress in recurrent spontaneous abortion (RSA)","volume":"133","author":"Shamsi","year":"2011","journal-title":"Indian J Med Res"}],"container-title":["Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X21000607?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X21000607?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:28:55Z","timestamp":1777620535000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1642431X21000607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["S1642431X21000607"],"URL":"https:\/\/doi.org\/10.1016\/j.repbio.2021.100539","relation":{},"ISSN":["1642-431X"],"issn-type":[{"value":"1642-431X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Label-free proteomics of spermatozoa identifies candidate protein markers of idiopathic recurrent pregnancy loss","name":"articletitle","label":"Article Title"},{"value":"Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.repbio.2021.100539","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"100539"}}