{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T12:30:00Z","timestamp":1773232200079,"version":"3.50.1"},"reference-count":50,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT BID 2019\\u20131180 \/ PICT-STARTUP-2022\\u20130008"],"award-info":[{"award-number":["PICT BID 2019\\u20131180 \/ PICT-STARTUP-2022\\u20130008"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 0761"],"award-info":[{"award-number":["PIP 0761"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003033","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021778","name":"Agencia Nacional De Promocion Cientifica Y Tecnologica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Biology"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.repbio.2025.101087","type":"journal-article","created":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T05:39:28Z","timestamp":1758951568000},"page":"101087","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity"],"prefix":"10.1016","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3280-3476","authenticated-orcid":false,"given":"Anabella Andrea","family":"Campagna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Carolina","family":"Fabra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelia","family":"Nikoloff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9901-9227","authenticated-orcid":false,"given":"Ana Cristina","family":"Carranza-Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.repbio.2025.101087_bib1","first-page":"869","article-title":"Environment to embryo: intersections of contaminant exposure and preimplantation embryo development in agricultural animals","volume":"107","author":"McGraw","year":"2022","journal-title":"Biol Reprod"},{"key":"10.1016\/j.repbio.2025.101087_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2019.106251","article-title":"Pregnancy loss in beef cattle: a meta-analysis","volume":"212","author":"Reese","year":"2020","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.repbio.2025.101087_bib3","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1007\/s11356-019-06980-4","article-title":"Impact of environmental contaminants on reproductive health of Male domestic ruminants: a review","volume":"27","author":"Guvvala","year":"2020","journal-title":"Environ Sci Pollut Res"},{"key":"10.1016\/j.repbio.2025.101087_bib4","doi-asserted-by":"crossref","first-page":"198","DOI":"10.3390\/jpm14020198","article-title":"Decrease in sperm parameters in the 21st century: obesity, lifestyle, or environmental factors? An updated narrative review","volume":"14","author":"Sciorio","year":"2024","journal-title":"J Pers Med"},{"key":"10.1016\/j.repbio.2025.101087_bib5","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0890-6238(03)00031-5","article-title":"The presence of environmental pollutants in the semen of farm animals (bull, ram, goat, and boar)","volume":"17","author":"Kamarianos","year":"2003","journal-title":"Reprod Toxicol"},{"key":"10.1016\/j.repbio.2025.101087_bib6","doi-asserted-by":"crossref","DOI":"10.1590\/1984-3143-ar2020-0041","article-title":"Effect of environmental contamination on female and Male gametes \u2013 a lesson from bovines","volume":"17","author":"Roth","year":"2020","journal-title":"Anim Reprod"},{"key":"10.1016\/j.repbio.2025.101087_bib7","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1146\/annurev-animal-021022-040629","article-title":"Sperm in the mammalian female reproductive tract: surfing through the tract to try to beat the odds","volume":"12","author":"Miller","year":"2024","journal-title":"Annu Rev Anim Biosci"},{"key":"10.1016\/j.repbio.2025.101087_bib8","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1051\/vetres:2002039","article-title":"Helminthic control on grazing ruminants and environmental risks in South America","volume":"33","author":"Suarez","year":"2002","journal-title":"Vet Res"},{"key":"10.1016\/j.repbio.2025.101087_bib9","first-page":"131","author":"Drobnis","year":"2017","journal-title":"Antimicrob Male Reprod"},{"key":"10.1016\/j.repbio.2025.101087_bib10","doi-asserted-by":"crossref","first-page":"113","DOI":"10.46311\/2318-0579.53.eUJ1428","article-title":"Efic\u00e1cia do controle qu\u00edmico de carrapatos rhipicephalus (Boophilus) microplus em bovinos leiteiros com uso de fluazuron: relato de caso","volume":"53","author":"Couto Filho","year":"2017","journal-title":"Rev Uning\u00e1"},{"key":"10.1016\/j.repbio.2025.101087_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/2815461","article-title":"Novel acaricidal drug fluazuron causes immunotoxicity via selective depletion of lymphocytes t CD8","volume":"2019","author":"Ribeiro","year":"2019","journal-title":"EvidBased Complement Altern Med"},{"key":"10.1016\/j.repbio.2025.101087_bib12","volume":"57","author":"WHO","year":"2009","journal-title":"Toxicol Eval Certain Vet Drug Residues Food"},{"key":"10.1016\/j.repbio.2025.101087_bib13","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1186\/s12917-021-03090-7","article-title":"Is the suckling period and application pattern relevant for fluazuron against tick infestation in cows and their suckling calves?","volume":"17","author":"Su\u00e1rez","year":"2021","journal-title":"BMC Vet Res"},{"key":"10.1016\/j.repbio.2025.101087_bib14","series-title":"Food and Nutrition Paper","first-page":"18","author":"Tantiyaswasdikul","year":"1997"},{"key":"10.1016\/j.repbio.2025.101087_bib15","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.theriogenology.2024.07.015","article-title":"Impact of fluazuron on oocyte maturation: May the antiparasitic affect bovine reproduction?","volume":"227","author":"Campagna","year":"2024","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib16","doi-asserted-by":"crossref","first-page":"4735","DOI":"10.7150\/thno.21662","article-title":"The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study","volume":"7","author":"Kang","year":"2017","journal-title":"Theranostics"},{"key":"10.1016\/j.repbio.2025.101087_bib17","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1530\/rep.1.01136","article-title":"A bovine oviduct epithelial cell suspension culture system suitable for studying embryo\u2013maternal interactions: morphological and functional characterization","volume":"132","author":"Rottmayer","year":"2006","journal-title":"Reproduction"},{"key":"10.1016\/j.repbio.2025.101087_bib18","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.theriogenology.2009.09.036","article-title":"In vitro systems for intercepting early embryo-maternal cross-talk in the bovine oviduct","volume":"73","author":"Ulbrich","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib19","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/0093-691X(86)90143-3","article-title":"Bovine in vitro fertilization with frozen-thawed semen","volume":"25","author":"Parrish","year":"1986","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.theriogenology.2013.08.005","article-title":"Bovine in vitro fertilization: in vitro oocyte maturation and sperm capacitation with heparin","volume":"81","author":"Parrish","year":"2014","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib21","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0093-691X(99)00162-4","article-title":"High bovine blastocyst development in a static in vitro production system using sofaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins","volume":"52","author":"Holm","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.mrgentox.2004.05.018","article-title":"Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP","volume":"563","author":"Kosmider","year":"2004","journal-title":"Mutat Res\/Genet Toxicol Environ Mutagen"},{"key":"10.1016\/j.repbio.2025.101087_bib23","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0014-4827(88)90265-0","article-title":"A simple technique for quantitation of low levels of DNA damage in individual cells","volume":"175","author":"Singh","year":"1988","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.repbio.2025.101087_bib24","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3390\/toxics12040270","article-title":"The comet assay as a tool in human biomonitoring studies of environmental and occupational exposure to Chemicals\u2014A systematic scoping review","volume":"12","author":"Ladeira","year":"2024","journal-title":"Toxics"},{"key":"10.1016\/j.repbio.2025.101087_bib25","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S1383-5718(99)00042-X","article-title":"Evaluation of DNA damage by the comet assay in shoe workers exposed to toluene and other organic solvents","volume":"441","author":"Pitarque","year":"1999","journal-title":"Mutat Res\/Genet Toxicol Environ Mutagen"},{"key":"10.1016\/j.repbio.2025.101087_bib26","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.vetpar.2005.07.004","article-title":"Tick, fly, and mosquito control\u2014lessons from the past, solutions for the future","volume":"132","author":"Peter","year":"2005","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.repbio.2025.101087_bib27","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1590\/S1984-29612014042","article-title":"Reassessment of the potential economic impact of cattle parasites in Brazil","volume":"23","author":"Grisi","year":"2014","journal-title":"Rev Bras De Parasitol Veter\u00e1ria"},{"key":"10.1016\/j.repbio.2025.101087_bib28","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.20506\/rst.13.4.816","article-title":"Insecticides and acaricides: resistance and environmental impact","volume":"13","author":"Kunz","year":"1994","journal-title":"Rev Sci Et Tech De l\u2019OIE"},{"key":"10.1016\/j.repbio.2025.101087_bib29","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.vetpar.2014.01.012","article-title":"First report of fluazuron resistance in rhipicephalus microplus: a field tick population resistant to six classes of acaricides","volume":"201","author":"Reck","year":"2014","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.repbio.2025.101087_bib30","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s10493-022-00713-y","article-title":"Resistance of the cattle tick rhipicephalus (Boophilus) microplus to fluazuron in Argentina","volume":"86","author":"Torrents","year":"2022","journal-title":"Exp Appl Acarol"},{"key":"10.1016\/j.repbio.2025.101087_bib31","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.theriogenology.2016.04.052","article-title":"Managing the reproductive performance of beef cows","volume":"86","author":"Diskin","year":"2016","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib32","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.theriogenology.2018.08.021","article-title":"Resynchronization improves reproductive efficiency of suckled bos Taurus beef cows subjected to spring-summer or autumn-winter breeding season in south Brazil","volume":"122","author":"Pessoa","year":"2018","journal-title":"Theriogenology"},{"issue":"9","key":"10.1016\/j.repbio.2025.101087_bib33","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1016\/j.theriogenology.2016.02.014","article-title":"Effects of label-dose permethrin administration in yearling beef cattle: I. Bull reproductive function and testicular histopathology","volume":"85","author":"Dohlman","year":"2016","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib34","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0093-691X(03)00174-2","article-title":"Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate","volume":"60","author":"Brito","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib35","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.3390\/ani15121808","article-title":"Sperm membrane: molecular implications and strategies for cryopreservation in productive species","volume":"15","author":"Castro","year":"2025","journal-title":"Animals"},{"key":"10.1016\/j.repbio.2025.101087_bib36","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0378-4320(01)00148-8","article-title":"The effect of sperm preparation and co-incubation time on in vitro fertilization of bos indicus oocytes","volume":"69","author":"Dode","year":"2002","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.repbio.2025.101087_bib37","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1292\/jvms.13-0607","article-title":"Estimation of the optimal timing of fertilization for embryo development of in vitro-matured bovine oocytes based on The Times of nuclear maturation and sperm penetration","volume":"76","author":"Koyama","year":"2014","journal-title":"J Vet Med Sci"},{"key":"10.1016\/j.repbio.2025.101087_bib38","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.theriogenology.2023.01.019","article-title":"In vitro adverse effects of amitraz on semen quality: consequences in bovine embryo development","volume":"199","author":"Carranza-Martin","year":"2023","journal-title":"Theriogenology"},{"key":"10.1016\/j.repbio.2025.101087_bib39","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1080\/10934529.2010.506092","article-title":"In vitro copper toxicity on rabbit spermatozoa motility, morphology and cell membrane integrity","volume":"45","author":"Roychoudhury","year":"2010","journal-title":"J Environ Sci Health Part A"},{"key":"10.1016\/j.repbio.2025.101087_bib40","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1080\/10934529.2010.500909","article-title":"Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrity in vitro","volume":"45","author":"Roychoudhury","year":"2010","journal-title":"J Environ Sci Health Part A"},{"key":"10.1016\/j.repbio.2025.101087_bib41","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0015-0282(03)00596-X","article-title":"Pronuclear morphology and chromosomal abnormalities as scoring criteria for embryo selection","volume":"80","author":"Gianaroli","year":"2003","journal-title":"Fertil Steril"},{"key":"10.1016\/j.repbio.2025.101087_bib42","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/mrd.1120240203","article-title":"Development of human Male pronucleus: ultrastructure and timing","volume":"24","author":"Tesarik","year":"1989","journal-title":"Gamete Res"},{"key":"10.1016\/j.repbio.2025.101087_bib43","first-page":"169","author":"Lamba","year":"2023","journal-title":"role Polariz Early Heterog Mamm first Cell fate Decis"},{"key":"10.1016\/j.repbio.2025.101087_bib44","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1093\/molehr\/gav068","article-title":"Polarity and cell division orientation in the cleavage embryo: from worm to human","volume":"22","author":"Ajduk","year":"2016","journal-title":"Mol Hum Reprod"},{"key":"10.1016\/j.repbio.2025.101087_bib45","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/S1472-6483(10)61651-3","article-title":"Pronuclear morphology predicts embryo development and chromosome constitution","volume":"8","author":"Balaban","year":"2004","journal-title":"Reprod Biomed Online"},{"key":"10.1016\/j.repbio.2025.101087_bib46","doi-asserted-by":"crossref","first-page":"802","DOI":"10.3390\/ani11030802","article-title":"Chromosome abnormalities and fertility in domestic bovids: a review","volume":"11","author":"Iannuzzi","year":"2021","journal-title":"Animals"},{"key":"10.1016\/j.repbio.2025.101087_bib47","first-page":"1","author":"Sutovsky","year":"1999","journal-title":"Paternal Contrib Mamm Zygote Fertil Sperm Egg Fusion"},{"key":"10.1016\/j.repbio.2025.101087_bib48","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1093\/humrep\/dem385","article-title":"The role of sperm proteasomes during sperm aster formation and early zygote development: implications for fertilization failure in humans","volume":"23","author":"Rawe","year":"2008","journal-title":"Hum Reprod"},{"key":"10.1016\/j.repbio.2025.101087_bib49","doi-asserted-by":"crossref","DOI":"10.1530\/REP-24-0008","article-title":"Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics","volume":"167","author":"Senn","year":"2024","journal-title":"Reproduction"},{"key":"10.1016\/j.repbio.2025.101087_bib50","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1051\/parasite\/2019026","article-title":"Benzoylphenyl ureas as veterinary antiparasitics. An overview and outlook with emphasis on efficacy, usage and resistance","volume":"26","author":"Junquera","year":"2019","journal-title":"Parasite"}],"container-title":["Reproductive Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X25000944?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1642431X25000944?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T15:48:54Z","timestamp":1773157734000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1642431X25000944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S1642431X25000944"],"URL":"https:\/\/doi.org\/10.1016\/j.repbio.2025.101087","relation":{},"ISSN":["1642-431X"],"issn-type":[{"value":"1642-431X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity","name":"articletitle","label":"Article Title"},{"value":"Reproductive Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.repbio.2025.101087","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"101087"}}