{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T06:28:22Z","timestamp":1766557702876,"version":"build-2065373602"},"reference-count":30,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Toxicology"],"published-print":{"date-parts":[[2011,7]]},"DOI":"10.1016\/j.reprotox.2011.05.004","type":"journal-article","created":{"date-parts":[[2011,5,23]],"date-time":"2011-05-23T01:06:09Z","timestamp":1306112769000},"page":"106-111","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"title":["Incomplete protection of genetic integrity of mature spermatozoa against oxidative stress"],"prefix":"10.1016","volume":"32","author":[{"given":"Joost O.","family":"Linschooten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Laubenthal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Cemeli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolf","family":"Baumgartner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ville E.","family":"Sipinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunnar","family":"Brunborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido R.M.M.","family":"Haenen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleni","family":"Fthenou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob J.","family":"Bried\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik J.","family":"van Schooten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger W.L.","family":"Godschalk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.reprotox.2011.05.004_bib0005","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/mutage\/geq024","article-title":"In vitro evaluation of baseline and induced DNA damage in human sperm exposed to benzo[a]pyrene or its metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide, using the comet assay","volume":"25","author":"Sipinen","year":"2010","journal-title":"Mutagenesis"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0010","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0015-0282(02)03294-6","article-title":"Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study","volume":"78","author":"Saleh","year":"2002","journal-title":"Fertil Steril"},{"issue":"1\u20132","key":"10.1016\/j.reprotox.2011.05.004_bib0015","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.mrfmmm.2008.12.014","article-title":"Use of spermatozoal mRNA profiles to study gene-environment interactions in human germ cells","volume":"667","author":"Linschooten","year":"2009","journal-title":"Mutat Res"},{"issue":"6","key":"10.1016\/j.reprotox.2011.05.004_bib0020","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0090-4295(96)00313-5","article-title":"Role of reactive oxygen species in male infertility","volume":"48","author":"Sharma","year":"1996","journal-title":"Urology"},{"issue":"2","key":"10.1016\/j.reprotox.2011.05.004_bib0025","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1046\/j.1365-2605.1997.00044.x","article-title":"Reactive oxygen species and human spermatozoa: physiology and pathology","volume":"20","author":"Griveau","year":"1997","journal-title":"Int J Androl"},{"issue":"4","key":"10.1016\/j.reprotox.2011.05.004_bib0030","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/S0094-0143(02)00081-2","article-title":"Role of oxidants in male infertility: rationale, significance, and treatment","volume":"29","author":"Agarwal","year":"2002","journal-title":"Urol Clin North Am"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1530\/jrf.0.1150001","article-title":"The Amoroso Lecture. The human spermatozoon \u2013 a cell in crisis?","volume":"115","author":"Aitken","year":"1999","journal-title":"J Reprod Fertil"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0040","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1530\/ror.0.0040031","article-title":"Origin of DNA damage in ejaculated human spermatozoa","volume":"4","author":"Sakkas","year":"1999","journal-title":"Rev Reprod"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0045","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.fertnstert.2004.06.056","article-title":"Impact of sperm morphology on DNA damage caused by oxidative stress induced by beta-nicotinamide adenine dinucleotide phosphate","volume":"83","author":"Said","year":"2005","journal-title":"Fertil Steril"},{"issue":"2","key":"10.1016\/j.reprotox.2011.05.004_bib0050","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0027-5107(95)00251-0","article-title":"Smoking and low antioxidant levels increase oxidative damage to sperm DNA","volume":"351","author":"Fraga","year":"1996","journal-title":"Mutat Res"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0055","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/humupd\/dml057","article-title":"Altered protamine expression and diminished spermatogenesis: what is the link?","volume":"13","author":"Carrell","year":"2007","journal-title":"Hum Reprod Update"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0060","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/hep.23668","article-title":"Mitochondrial superoxide dismutase and glutathione peroxidase in idiosyncratic drug-induced liver injury","volume":"52","author":"Lucena","year":"2010","journal-title":"Hepatology"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0065","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0014-4827(88)90265-0","article-title":"A simple technique for quantitation of low levels of DNA damage in individual cells","volume":"175","author":"Singh","year":"1988","journal-title":"Exp Cell Res"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0070","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.rmed.2008.10.007","article-title":"Antioxidant status associated with inflammation in sarcoidosis: a potential role for antioxidants","volume":"103","author":"Boots","year":"2009","journal-title":"Respir Med"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0075","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0378-4347(00)83313-7","article-title":"Fluorometric determination of total vitamin C in whole blood by high-performance liquid chromatography with pre-column derivatization","volume":"305","author":"Speek","year":"1984","journal-title":"J Chromatogr"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0080","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/01485010701216526","article-title":"The preparation of human spermatozoal RNA for clinical analysis","volume":"53","author":"Goodrich","year":"2007","journal-title":"Arch Androl"},{"issue":"2","key":"10.1016\/j.reprotox.2011.05.004_bib0085","doi-asserted-by":"crossref","first-page":"144","DOI":"10.3164\/jcbn.08-251","article-title":"Ascorbic acid in human seminal plasma: determination and its relationship to sperm quality","volume":"45","author":"Colagar","year":"2009","journal-title":"J Clin Biochem Nutr"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0155","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/aja.2010.68","article-title":"Apoptosis and DNA damage in human spermatozoa","volume":"13","author":"Aitken","year":"2011","journal-title":"Asian J Androl."},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0095","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/molehr\/gap059","article-title":"On the possible origins of DNA damage in human spermatozoa","volume":"16","author":"Aitken","year":"2010","journal-title":"Mol Hum Reprod"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0100","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1097\/GCO.0b013e3282fc9c1e","article-title":"Cigarette smoking and reproductive function","volume":"20","author":"Soares","year":"2008","journal-title":"Curr Opin Obstet Gynecol"},{"issue":"11","key":"10.1016\/j.reprotox.2011.05.004_bib0105","doi-asserted-by":"crossref","first-page":"5103","DOI":"10.1158\/0008-5472.CAN-07-0279","article-title":"Mainstream tobacco smoke causes paternal germ-line DNA mutation","volume":"67","author":"Yauk","year":"2007","journal-title":"Cancer Res"},{"issue":"2","key":"10.1016\/j.reprotox.2011.05.004_bib0110","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.fertnstert.2008.07.1715","article-title":"Role of male factor in early recurrent embryo loss: do antioxidants have any effect?","volume":"92","author":"Gil-Villa","year":"2009","journal-title":"Fertil Steril"},{"issue":"24","key":"10.1016\/j.reprotox.2011.05.004_bib0115","doi-asserted-by":"crossref","first-page":"11003","DOI":"10.1073\/pnas.88.24.11003","article-title":"Ascorbic acid protects against endogenous oxidative DNA damage in human sperm","volume":"88","author":"Fraga","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"10.1016\/j.reprotox.2011.05.004_bib0120","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1093\/humrep\/deh725","article-title":"Antioxidant intake is associated with semen quality in healthy men","volume":"20","author":"Eskenazi","year":"2005","journal-title":"Hum Reprod"},{"issue":"6","key":"10.1016\/j.reprotox.2011.05.004_bib0125","doi-asserted-by":"crossref","first-page":"654","DOI":"10.2164\/jandrol.05016","article-title":"Prevention of oxidative stress injury to sperm","volume":"26","author":"Agarwal","year":"2005","journal-title":"J Androl"},{"issue":"1","key":"10.1016\/j.reprotox.2011.05.004_bib0130","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0891-5849(93)90508-R","article-title":"Ascorbate free radical as a marker of oxidative stress: an EPR study","volume":"14","author":"Buettner","year":"1993","journal-title":"Free Radic Biol Med"},{"issue":"5","key":"10.1016\/j.reprotox.2011.05.004_bib0135","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1590\/S1677-55382007000500002","article-title":"Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis","volume":"33","author":"Cocuzza","year":"2007","journal-title":"Int Braz J Urol"},{"issue":"3","key":"10.1016\/j.reprotox.2011.05.004_bib0140","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0015-0282(03)00756-8","article-title":"Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility","volume":"80","author":"Wang","year":"2003","journal-title":"Fertil Steril"},{"issue":"10","key":"10.1016\/j.reprotox.2011.05.004_bib0145","doi-asserted-by":"crossref","first-page":"4675","DOI":"10.1073\/pnas.0907705107","article-title":"Negative feedback control of HIF-1 through REDD1-regulated ROS suppresses tumorigenesis","volume":"107","author":"Horak","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"issue":"8","key":"10.1016\/j.reprotox.2011.05.004_bib0150","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1210\/jc.2007-2616","article-title":"Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa","volume":"93","author":"Koppers","year":"2008","journal-title":"J Clin Endocrinol Metab"}],"container-title":["Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623811001535?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623811001535?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:31:41Z","timestamp":1760189501000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890623811001535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["S0890623811001535"],"URL":"https:\/\/doi.org\/10.1016\/j.reprotox.2011.05.004","relation":{},"ISSN":["0890-6238"],"issn-type":[{"type":"print","value":"0890-6238"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Incomplete protection of genetic integrity of mature spermatozoa against oxidative stress","name":"articletitle","label":"Article Title"},{"value":"Reproductive Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reprotox.2011.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}