{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T10:52:02Z","timestamp":1792579922271,"version":"4.5.0"},"reference-count":57,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004434","name":"University of Florence","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Toxicology"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1016\/j.reprotox.2021.10.004","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T20:35:44Z","timestamp":1633984544000},"page":"51-60","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"special_numbering":"C","title":["Novel methods to detect ROS in viable spermatozoa of native semen samples"],"prefix":"10.1016","volume":"106","author":[{"given":"Lucrezia","family":"Riley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumaima","family":"Ammar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6192-6902","authenticated-orcid":false,"given":"Tommaso","family":"Mello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4027-4693","authenticated-orcid":false,"given":"Lisa","family":"Giovannelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Vignozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Muratori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.reprotox.2021.10.004_bib0005","article-title":"Novel concepts in the aetiology of male reproductive impairment","volume":"5","author":"Tournaye","year":"2017","journal-title":"Lancet Diabetes Endocrinol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0010","series-title":"EAU Guidel. Male Infertil.","article-title":"European association of urology guidelines 2017 on male infertility","author":"Jungwirth","year":"2017"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0015","doi-asserted-by":"crossref","DOI":"10.1097\/01.XHA.0000397686.82729.09","article-title":"Unexplained male infertility: potential causes and management","volume":"1","author":"Hamada","year":"2011","journal-title":"Hum. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0020","doi-asserted-by":"crossref","DOI":"10.3390\/antiox9020111","article-title":"The importance of oxidative stress in determining the functionality of mammalian spermatozoa: a two-edged sword","volume":"9","author":"Aitken","year":"2020","journal-title":"Antioxidants"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0025","doi-asserted-by":"crossref","DOI":"10.1007\/s00018-019-03253-8","article-title":"Oxidative stress and male infertility: current knowledge of pathophysiology and role of antioxidant therapy in disease management","volume":"77","author":"Barati","year":"2020","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0030","doi-asserted-by":"crossref","DOI":"10.3390\/antiox10010092","article-title":"Mitochondrial reactive oxygen species (ROS) production alters sperm quality","volume":"10","author":"Chianese","year":"2021","journal-title":"Antioxidants"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0035","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2119\/molmed.2014.00158","article-title":"Investigation on the origin of sperm DNA fragmentation: role of apoptosis, immaturity and oxidative stress","volume":"21","author":"Muratori","year":"2015","journal-title":"Mol. Med."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0040","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-21664-1_6","article-title":"Sperm DNA fragmentation: consequences for reproduction","author":"Simon","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0045","doi-asserted-by":"crossref","DOI":"10.3390\/diseases5010009","article-title":"The effect of natural antioxidants and phytocompounds on seminal oxidative stress","volume":"5","author":"Adewoyin","year":"2017","journal-title":"Diseases"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0050","doi-asserted-by":"crossref","DOI":"10.1186\/s12958-018-0431-1","article-title":"Radiations and male fertility","volume":"16","author":"Kesari","year":"2018","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0055","doi-asserted-by":"crossref","DOI":"10.2164\/jandrol.111.013193","article-title":"Diabetes mellitus and minireview sperm parameters","volume":"33","author":"La Vignera","year":"2012","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.urology.2008.07.053","article-title":"Role of oxidative stress in pathogenesis of varicocele and infertility","author":"Agarwal","year":"2009","journal-title":"Urology"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0065","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.gyobfe.2009.03.029","article-title":"Teratozoospermia at the time of intracytoplasmic morphologically selected sperm injection (IMSI)","volume":"37","author":"Belloc","year":"2009","journal-title":"Gynecol. Obstet. Fertil."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0070","article-title":"Antioxidants for male subfertility","volume":"2019","author":"Smits","year":"2019","journal-title":"Cochrane Database Syst. Rev."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0075","article-title":"Laboratory assessment of oxidative stress in semen","volume":"16","author":"Agarwal","year":"2018","journal-title":"Arab J. Urol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0080","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M112.366690","article-title":"Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase","volume":"287","author":"Aitken","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0085","doi-asserted-by":"crossref","DOI":"10.1111\/j.2047-2927.2012.00056.x","article-title":"On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid","volume":"1","author":"Aitken","year":"2013","journal-title":"Andrology"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.freeradbiomed.2015.01.015","article-title":"Boronate probes for the detection of hydrogen peroxide release from human spermatozoa","volume":"81","author":"Purdey","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0095","article-title":"Fluorescent probes for the detection of reactive oxygen species in human spermatozoa","volume":"22","author":"Escada-Rebelo","year":"2020","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0100","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/dey221","article-title":"Peroxiredoxin 6 is the primary antioxidant enzyme for the maintenance of viability and DNA integrity in human spermatozoa","volume":"33","author":"Fernandez","year":"2018","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0105","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/dev015","article-title":"Mitochondrial permeability transition increases reactive oxygen species production and induces DNA fragmentation in human spermatozoa","volume":"30","author":"Treulen","year":"2015","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0110","series-title":"World Health Organization Laboratory Manual for the Examination and Processing of Human Semen","author":"WHO","year":"2010"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymeth.2016.06.008","article-title":"Quantifying ROS levels using CM-H2DCFDA and HyPer","volume":"109","author":"Oparka","year":"2016","journal-title":"Methods"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0120","doi-asserted-by":"crossref","DOI":"10.1093\/molehr\/gam046","article-title":"Characterization of M540 bodies in human semen: evidence that they are apoptotic bodies","author":"Marchiani","year":"2007","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0125","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/j.2047-2927.2014.00208.x","article-title":"Characterization and sorting of flow cytometric populations in human semen","volume":"2","author":"Marchiani","year":"2014","journal-title":"Andrology"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0130","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1002\/j.1939-4640.2000.tb02136.x","article-title":"Uteroglobin and transglutaminase modulate human sperm functions","volume":"21","author":"Luconi","year":"2000","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0135","doi-asserted-by":"crossref","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0140","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-62703-038-0_13","article-title":"Sperm DNA damage measured by comet assay","volume":"927","author":"Simon","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0145","doi-asserted-by":"crossref","DOI":"10.1002\/j.1939-4640.2003.tb02641.x","article-title":"The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation","volume":"24","author":"Fern\u00e1ndez","year":"2003","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0150","doi-asserted-by":"crossref","DOI":"10.1002\/j.1939-4640.2004.tb02858.x","article-title":"Annexin V binding and merocyanine staining fail to detect human sperm capacitation","volume":"25","author":"Muratori","year":"2004","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0155","doi-asserted-by":"crossref","DOI":"10.1111\/j.1365-2605.2010.01118.x","article-title":"Sumo1-ylation of human spermatozoa and its relationship with semen quality","volume":"34","author":"Marchiani","year":"2011","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.taap.2012.04.030","article-title":"Rapid generation of mitochondrial superoxide induces mitochondrion-dependent but caspase-independent cell death in hippocampal neuronal cells that morphologically resembles necroptosis","volume":"262","author":"Fukui","year":"2012","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0165","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/deu204","article-title":"Potential importance of transition metals in the induction of DNA damage by sperm preparation media","volume":"29","author":"Aitken","year":"2014","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0170","doi-asserted-by":"crossref","DOI":"10.1097\/MD.0000000000003624","article-title":"Variation of DNA fragmentation levels during density gradient sperm selection for assisted reproduction techniques: a possible new male predictive parameter of pregnancy?","volume":"95","author":"Muratori","year":"2016","journal-title":"Medicine (Baltimore)"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0175","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-43981-2","article-title":"Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa","volume":"9","author":"Muratori","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0180","doi-asserted-by":"crossref","DOI":"10.1093\/humrep\/deq214","article-title":"Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria","volume":"25","author":"Aitken","year":"2010","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0185","doi-asserted-by":"crossref","DOI":"10.1159\/000475016","article-title":"A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species","volume":"28","author":"Shekarriz","year":"1995","journal-title":"Eur. Urol."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0190","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0006-291X(03)00641-7","article-title":"Method to overcome photoreaction, a serious drawback to the use of dichlorofluorescin in evaluation of reactive oxygen species","volume":"304","author":"Afzal","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0195","doi-asserted-by":"crossref","first-page":"28161","DOI":"10.1074\/jbc.274.40.28161","article-title":"Phenoxyl free radical formation during the oxidation of the fluorescent dye 2\u2019,7\u2019-dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements","volume":"274","author":"Rota","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0200","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0891-5849(98)00174-9","article-title":"Photoreduction of the fluorescent dye 2\u2019-7\u2019-dichlorofluorescein: a spin trapping and direct electron spin resonance study with implications for oxidative stress measurements","volume":"26","author":"Marchesi","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.theriogenology.2012.05.008","article-title":"Improvement of boar sperm cryosurvival by using single-layer colloid centrifugation prior freezing","volume":"78","author":"Martinez-Alborcia","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0210","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0036550","article-title":"Detrimental effects of non-functional spermatozoa on the freezability of functional spermatozoa from Boar Ejaculate","volume":"7","author":"Martinez-Alborcia","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0215","doi-asserted-by":"crossref","DOI":"10.1071\/RD12204","article-title":"The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function","volume":"25","author":"Kiernan","year":"2013","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0220","doi-asserted-by":"crossref","DOI":"10.1071\/RD14043","article-title":"Free-radical production after post-thaw incubation of ram spermatozoa is related to decreased in vivo fertility","volume":"27","author":"Del Olmo","year":"2015","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0225","doi-asserted-by":"crossref","DOI":"10.3168\/jds.2012-6484","article-title":"Reducing sperm concentration is critical to limiting the oxidative stress challenge in liquid bull semen","volume":"96","author":"Murphy","year":"2013","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0230","article-title":"Evaluation of intracellular location of reactive oxygen species in solea senegalensis spermatozoa","volume":"2018","author":"Valcarce","year":"2018","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0235","doi-asserted-by":"crossref","DOI":"10.1530\/REP-08-0357","article-title":"Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa","volume":"137","author":"Mart\u00ednez-Pastor","year":"2009","journal-title":"Reproduction"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0240","doi-asserted-by":"crossref","DOI":"10.1007\/s10561-020-09828-1","article-title":"The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11\u2013q13 region in human spermatozoa","volume":"21","author":"Khosravizadeh","year":"2020","journal-title":"Cell Tissue Bank."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0245","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/8213071","article-title":"Sperm oxidative stress is detrimental to embryo development: a dose-dependent study model and a new and more sensitive oxidative status evaluation","volume":"2016","author":"De Castro","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0250","article-title":"Ram semen quality can be assessed by flow cytometry several hours after post-fixation","author":"Va\u0161\u00ed\u010dek","year":"2020","journal-title":"Zygote"},{"key":"10.1016\/j.reprotox.2021.10.004_bib0255","doi-asserted-by":"crossref","DOI":"10.1038\/nprot.2007.327","article-title":"Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy","volume":"2","author":"Mukhopadhyay","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0260","doi-asserted-by":"crossref","first-page":"15038","DOI":"10.1073\/pnas.0601945103","article-title":"Selective fluorescent imaging of superoxide in vivo using ethidium-based probes","volume":"103","author":"Robinson","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0265","doi-asserted-by":"crossref","DOI":"10.1210\/jc.2007-2616","article-title":"Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa","author":"Koppers","year":"2008","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0270","doi-asserted-by":"crossref","DOI":"10.1007\/s10815-017-0912-8","article-title":"Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future","volume":"34","author":"Gosalvez","year":"2017","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0275","doi-asserted-by":"crossref","DOI":"10.1002\/j.1939-4640.2003.tb02670.x","article-title":"Spontaneous DNA fragmentation in swim-up selected human spermatozoa during long term incubation","volume":"24","author":"Muratori","year":"2003","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0280","doi-asserted-by":"crossref","DOI":"10.1002\/j.1939-4640.1987.tb00973.x","article-title":"Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa superoxide dismutase as major enzyme protectant against oxygen toxicity","volume":"8","author":"Alvarez","year":"1987","journal-title":"J. Androl."},{"key":"10.1016\/j.reprotox.2021.10.004_bib0285","doi-asserted-by":"crossref","DOI":"10.1016\/S0015-0282(16)58233-8","article-title":"Impact of in vitro fertilization culture media on peroxidative damage to human spermatozoa","volume":"65","author":"Gomez","year":"1996","journal-title":"Fertil. Steril."}],"container-title":["Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089062382100160X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089062382100160X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:56:34Z","timestamp":1760190994000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S089062382100160X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":57,"alternative-id":["S089062382100160X"],"URL":"https:\/\/doi.org\/10.1016\/j.reprotox.2021.10.004","relation":{},"ISSN":["0890-6238"],"issn-type":[{"value":"0890-6238","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Novel methods to detect ROS in viable spermatozoa of native semen samples","name":"articletitle","label":"Article Title"},{"value":"Reproductive Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reprotox.2021.10.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}