{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:03:07Z","timestamp":1772816587915,"version":"3.50.1"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"am","delay-in-days":294,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Toxicology"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.reprotox.2023.108363","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T12:37:02Z","timestamp":1678883822000},"page":"108363","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Ex vivo effects of 17\u03b2-estradiol on the prepubertal rat testis"],"prefix":"10.1016","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5430-8946","authenticated-orcid":false,"given":"Marion","family":"Rouge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u00ebva","family":"Drouault","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Hanoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christelle","family":"Delalande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Boura\u00efma-Lelong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.reprotox.2023.108363_bib1","doi-asserted-by":"crossref","first-page":"3898","DOI":"10.1210\/endo.141.10.7723","article-title":"Comparative effects of neonatal exposure of male rats to potent and weak (environmental) estrogens on spermatogenesis at puberty and the relationship to adult testis size and fertility: evidence for stimulatory effects of low estrogen levels","volume":"141","author":"Atanassova","year":"2000","journal-title":"Endocrinology"},{"key":"10.1016\/j.reprotox.2023.108363_bib2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/1477-7827-8-72","article-title":"Effect of high intratesticular estrogen on global gene expression and testicular cell number in rats","volume":"8","author":"Balasinor","year":"2010","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib3","doi-asserted-by":"crossref","first-page":"16878","DOI":"10.1038\/s41598-017-17204-5","article-title":"QuPath: Open source software for digital pathology image analysis","volume":"7","author":"Bankhead","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.reprotox.2023.108363_bib4","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0960-0760(95)00022-R","article-title":"Differentiation of adult Leydig cells","volume":"53","author":"Benton","year":"1995","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.reprotox.2023.108363_bib5","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1093\/toxsci\/54.1.138","article-title":"The estrogen receptor relative binding affinities of 188 natural and xenochemicals: structural diversity of ligands","volume":"54","author":"Blair","year":"2000","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.reprotox.2023.108363_bib6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1530\/JME-11-0105","article-title":"17\u03b2-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules","volume":"48","author":"Bois","year":"2012","journal-title":"J. Mol. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1677\/JME-10-0041","article-title":"Age- and cell-related gene expression of aromatase and estrogen receptors in the rat testis","volume":"45","author":"Bois","year":"2010","journal-title":"J. Mol. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib8","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.reprotox.2022.06.004","article-title":"Effects of bisphenol A and estradiol in adult rat testis after prepubertal and pubertal exposure","volume":"111","author":"Brouard","year":"2022","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.reprotox.2023.108363_bib9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.reprotox.2016.05.003","article-title":"Differential effects of bisphenol A and estradiol on rat spermatogenesis\u2019 establishment","volume":"63","author":"Brouard","year":"2016","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.reprotox.2023.108363_bib10","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1046\/j.1365-2605.1999.00172.x","article-title":"Sources of oestrogen in the testis and reproductive tract of the male","volume":"22","author":"Carreau","year":"1999","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.reprotox.2023.108363_bib11","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.mce.2005.11.021","article-title":"Aromatase and estrogen receptors in male reproduction","volume":"246","author":"Carreau","year":"2006","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib12","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1098\/rstb.2009.0113","article-title":"Aromatase, oestrogens and human male reproduction","volume":"365","author":"Carreau","year":"2010","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"10.1016\/j.reprotox.2023.108363_bib13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mce.2009.01.023","article-title":"Leydig cells: from stem cells to aging","volume":"306","author":"Chen","year":"2009","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1124\/pr.110.002790","article-title":"The blood-testis barrier and its implications for male contraception","volume":"64","author":"Cheng","year":"2012","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.reprotox.2023.108363_bib15","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.3390\/cells9092115","article-title":"Role of GPER-mediated signaling in testicular functions and tumorigenesis","volume":"9","author":"Chimento","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.reprotox.2023.108363_bib16","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.mce.2010.01.035","article-title":"17\u03b2-Estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER\u03b1","volume":"320","author":"Chimento","year":"2010","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib17","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1111\/j.1365-2605.2010.01100.x","article-title":"Gper and ESRs are expressed in rat round spermatids and mediate oestrogen-dependent rapid pathways modulating expression of cyclin B1 and Bax","volume":"34","author":"Chimento","year":"2011","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.reprotox.2023.108363_bib18","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1152\/physrev.00018.2016","article-title":"Estrogens in male physiology","volume":"97","author":"Cooke","year":"2017","journal-title":"Physiol. Rev."},{"issue":"8","key":"10.1016\/j.reprotox.2023.108363_bib19","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1210\/endo.141.8.7596","article-title":"Estrogenic induction of spermatogenesis in the hypogonadal mouse","volume":"141","author":"Ebling","year":"2000","journal-title":"Endocrinoloy"},{"key":"10.1016\/j.reprotox.2023.108363_bib20","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1677\/joe.0.1530485","article-title":"Immunolocalisation of oestrogen receptor-\u03b1 within the testis and excurrent ducts of the rat and marmoset monkey from perinatal life to adulthood","volume":"153","author":"Fisher","year":"1997","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0002-9440(10)64736-0","article-title":"Overexpression of aromatase leads to development of testicular leydig cell tumors","volume":"156","author":"Fowler","year":"2000","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.reprotox.2023.108363_bib22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.acthis.2004.04.002","article-title":"Dose- and photoperiod-dependent effects of 17\u03b2-estradiol and the anti-estrogen ICI 182,780 on testicular structure, acceleration of spermatogenesis, and aromatase immunoexpression in immature bank voles","volume":"106","author":"Gancarczyk","year":"2004","journal-title":"Acta Histochem"},{"key":"10.1016\/j.reprotox.2023.108363_bib23","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1210\/en.2002-220710","article-title":"Sertoli and germ cell development in hypogonadal (hpg) mice expressing transgenic follicle-stimulating hormone alone or in combination with testosterone","volume":"144","author":"Haywood","year":"2003","journal-title":"Endocrinology"},{"key":"10.1016\/j.reprotox.2023.108363_bib24","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/jemt.20783","article-title":"Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes","volume":"73","author":"Hermo","year":"2010","journal-title":"Microsc. Res. Tech."},{"key":"10.1016\/j.reprotox.2023.108363_bib25","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0303-7207(01)00412-9","article-title":"Oestrogen, its receptors and function in the male reproductive tract \u2014 a review","volume":"178","author":"Hess","year":"2001","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib26","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1210\/er.2018-00087","article-title":"Estrogen receptors: new directions in the new millennium","volume":"39","author":"Hewitt","year":"2018","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.reprotox.2023.108363_bib27","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13293-019-0219-9","article-title":"Estrogen receptor profiles across tissues from male and female Rattus norvegicus","volume":"10","author":"Hutson","year":"2019","journal-title":"Biol. Sex. Differ."},{"key":"10.1016\/j.reprotox.2023.108363_bib28","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1210\/endo-122-1-34","article-title":"Induction of precocious maturation of spermatogenesis in infant rats by human menopausal gonadotropin and inhibition by simultaneous administration of gonadotropins and testosterone","volume":"122","author":"Kula","year":"1988","journal-title":"Endocrinology"},{"key":"10.1016\/j.reprotox.2023.108363_bib29","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0303-7207(01)00415-4","article-title":"Estradiol enhances the stimulatory effect of FSH on testicular maturation and contributes to precocious initiation of spermatogenesis","volume":"178","author":"Kula","year":"2001","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.reprotox.2023.108363_bib30","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1071\/RD08144","article-title":"Oestradiol-induced spermatogenesis requires a functional androgen receptor","volume":"20","author":"Lim","year":"2008","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.reprotox.2023.108363_bib31","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/3855","article-title":"Cyclin A1 is required for meiosis in the male mouse","volume":"20","author":"Liu","year":"1998","journal-title":"Nat. Genet."},{"key":"10.1016\/j.reprotox.2023.108363_bib32","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1095\/biolreprod59.1.84","article-title":"Developmental schedule of the postnatal rat testis determined by flow cytometry","volume":"59","author":"Malkov","year":"1998","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.reprotox.2023.108363_bib33","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4161\/spmg.1.3.17998","article-title":"A study to assess the assembly of a functional blood-testis barrier in developing rat testes","volume":"1","author":"Mok","year":"2011","journal-title":"Spermatogenesis"},{"issue":"3","key":"10.1016\/j.reprotox.2023.108363_bib34","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1210\/edrv.22.3.0431","article-title":"Estrogen and spermatogenesis","volume":"22","author":"O\u2019Donnell","year":"2001","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.reprotox.2023.108363_bib35","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/ar.1092030408","article-title":"Proliferation of sertoli cells in fetal and postnatal rats: a quantitative autoradiographic study","volume":"203","author":"Orth","year":"1982","journal-title":"Anat. Rec."},{"key":"10.1016\/j.reprotox.2023.108363_bib36","first-page":"1261","article-title":"Localization of androgen and estrogen receptors in rat and primate tissues","author":"Pelletier","year":"2000","journal-title":"Histol. Histopathol."},{"key":"10.1016\/j.reprotox.2023.108363_bib37","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1177\/0192623314547279","article-title":"Postnatal development of the testis in the rat: morphologic study and correlation of morphology to neuroendocrine parameters","volume":"43","author":"Picut","year":"2015","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.reprotox.2023.108363_bib38","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1126\/science.1106943","article-title":"A transmembrane intracellular estrogen receptor mediates rapid cell signaling","volume":"307","author":"Revankar","year":"2005","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.reprotox.2023.108363_bib39","first-page":"1266","article-title":"Clusterin signals via ApoER2\/VLDLR and induces meiosis of male germ cells","volume":"9","author":"Riaz","year":"2017","journal-title":"Am. J. Transl. Res."},{"key":"10.1016\/j.reprotox.2023.108363_bib40","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1046\/j.1439-0272.2003.00540.x","article-title":"Development of the foetal and neonatal testis","volume":"35","author":"Rouiller-Fabre","year":"2003","journal-title":"Andrologia"},{"key":"10.1016\/j.reprotox.2023.108363_bib41","first-page":"265","article-title":"Cell detection with star-convex polygons","volume":"11071","author":"Schmidt","year":"2018","journal-title":"ArXiv180603535 Cs"},{"key":"10.1016\/j.reprotox.2023.108363_bib42","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1210\/endo-76-6-1184","article-title":"Effect of estrogen or testosterone on initiation and maintenance of spermatogenesis in the rat","volume":"76","author":"Steinberger","year":"1965","journal-title":"Endocrinology"},{"key":"10.1016\/j.reprotox.2023.108363_bib43","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1210\/en.2008-1311","article-title":"Increased exposure to estrogens disturbs maturation, steroidogenesis, and cholesterol homeostasis via estrogen receptor \u03b1 in adult mouse leydig cells","volume":"150","author":"Strauss","year":"2009","journal-title":"Endocrinology"},{"issue":"1","key":"10.1016\/j.reprotox.2023.108363_bib44","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1242\/dev.122.1.53","article-title":"A distinct cyclin A is expressed in germ cells in the mouse","volume":"122","author":"Sweeney","year":"1996","journal-title":"Development"},{"key":"10.1016\/j.reprotox.2023.108363_bib45","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1210\/en.2004-1064","article-title":"Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells","volume":"146","author":"Thomas","year":"2005","journal-title":"Endocrinology"},{"key":"10.1016\/j.reprotox.2023.108363_bib46","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0092425","article-title":"Activation of GPER-1 estradiol receptor downregulates production of testosterone in isolated rat leydig cells and adult human testis","volume":"9","author":"Vaucher","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.reprotox.2023.108363_bib47","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bbrc.2017.02.092","article-title":"Effect of tamoxifen on spermatogenesis and testicular steroidogenesis","volume":"486","author":"Verma","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.reprotox.2023.108363_bib48","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.repbio.2013.07.005","article-title":"Estradiol and testosterone inhibit rat seminiferous tubule development in a hormone-specific way","volume":"13","author":"Walczak-J\u0119drzejowska","year":"2013","journal-title":"Reprod. Biol."},{"key":"10.1016\/j.reprotox.2023.108363_bib49","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1111\/j.1365-2605.2004.00480.x","article-title":"The A-type cyclins and the meiotic cell cycle in mammalian male germ cells","volume":"27","author":"Wolgemuth","year":"2004","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.reprotox.2023.108363_bib50","doi-asserted-by":"crossref","first-page":"430","DOI":"10.3389\/fphys.2017.00430","article-title":"Insights into the development of the adult leydig cell lineage from stem leydig cells","volume":"8","author":"Ye","year":"2017","journal-title":"Front. Physiol."},{"key":"10.1016\/j.reprotox.2023.108363_bib51","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1007\/s00018-019-03101-9","article-title":"The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis","volume":"76","author":"Zhou","year":"2019","journal-title":"Cell. Mol. Life Sci."}],"container-title":["Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623823000369?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623823000369?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:59:10Z","timestamp":1760191150000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890623823000369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":51,"alternative-id":["S0890623823000369"],"URL":"https:\/\/doi.org\/10.1016\/j.reprotox.2023.108363","relation":{},"ISSN":["0890-6238"],"issn-type":[{"value":"0890-6238","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ex vivo effects of 17\u03b2-estradiol on the prepubertal rat testis","name":"articletitle","label":"Article Title"},{"value":"Reproductive Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reprotox.2023.108363","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108363"}}