{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:04:32Z","timestamp":1792681472935,"version":"4.5.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Toxicology"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.reprotox.2025.108980","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T11:17:12Z","timestamp":1751023032000},"page":"108980","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Deep RNA analyses for BeWo cells and placentae of HIV positive pregnant women treated with lopinavir\/ritonavir"],"prefix":"10.1016","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0681-5123","authenticated-orcid":false,"given":"Shi","family":"Zou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuechen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinli","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songjie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"48","key":"10.1016\/j.reprotox.2025.108980_bib1","article-title":"HIV infections and HIV testing during pregnancy, Germany, 1993 to 2016","volume":"24","author":"Marcus","year":"2019","journal-title":"Eur. Surveill. Bull. Eur. sur Les. Mal. Transm. = Eur. Commun. Dis. Bull."},{"issue":"1","key":"10.1016\/j.reprotox.2025.108980_bib2","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1186\/s12879-020-05516-3","article-title":"Preventing mother to child transmission of HIV: lessons learned from China","volume":"20","author":"Dong","year":"2020","journal-title":"BMC Infect. Dis."},{"issue":"10","key":"10.1016\/j.reprotox.2025.108980_bib3","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1111\/1471-0528.12012","article-title":"Predictors of elective pregnancy termination among women diagnosed with HIV during pregnancy in two regions of China, 2004-2010","volume":"120","author":"Liang","year":"2013","journal-title":"BJOG Int. J. Obstet. Gynaecol."},{"issue":"47","key":"10.1016\/j.reprotox.2025.108980_bib4","doi-asserted-by":"crossref","DOI":"10.3346\/jkms.2018.33.e296","article-title":"Pregnancy rates and outcomes of HIV-infected women in Korea","volume":"33","author":"Choi","year":"2018","journal-title":"J. Korean Med. Sci."},{"issue":"9","key":"10.1016\/j.reprotox.2025.108980_bib5","doi-asserted-by":"crossref","first-page":"e385","DOI":"10.1016\/S2352-3018(15)00079-X","article-title":"Ukraine European Collaborative Study in E: pregnancy outcomes in HIV-positive women in Ukraine, 2000-12 (European Collaborative Study in EuroCoord): an observational cohort study","volume":"2","author":"Bagkeris","year":"2015","journal-title":"Lancet HIV"},{"issue":"3","key":"10.1016\/j.reprotox.2025.108980_bib6","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1080\/09540121.2022.2039358","article-title":"Adverse pregnancy outcomes among pregnant women living with HIV in Hubei province, China: prevalence and risk factors","volume":"35","author":"Wu","year":"2023","journal-title":"AIDS Care"},{"issue":"11","key":"10.1016\/j.reprotox.2025.108980_bib7","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s40262-014-0185-7","article-title":"Protecting the fetus against HIV infection: a systematic review of placental transfer of antiretrovirals","volume":"53","author":"McCormack","year":"2014","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.reprotox.2025.108980_bib8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s12879-016-1400-y","article-title":"Maternal and foetal outcomes among 4118 women with HIV infection treated with lopinavir\/ritonavir during pregnancy: analysis of population-based surveillance data from the national study of HIV in pregnancy and childhood in the United Kingdom and Ireland","volume":"16","author":"Tookey","year":"2016","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.reprotox.2025.108980_bib9","unstructured":"China HCotPsRo: Code of Practice for prevention of mother-to-child transmission of HIV, Syphilis and Hepatitis B (2020 edition). November 25, 2020. Available at: http:\/\/www.nhc.gov.cn\/fys\/s3581\/202011\/fc7b46b2b48b45a69bd390ae3a62d065.shtml."},{"issue":"3","key":"10.1016\/j.reprotox.2025.108980_bib10","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1002\/ijgo.12532","article-title":"Risk factors associated with preterm delivery and low delivery weight among HIV-exposed neonates in China","volume":"142","author":"Wang","year":"2018","journal-title":"Int. J. Gynaecol. Obstet. Off. Organ Int. Fed. Gynaecol. Obstet."},{"key":"10.1016\/j.reprotox.2025.108980_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2023.1158962","article-title":"Adverse pregnancy outcomes associated with antiretroviral therapy initiated before pregnancy and during pregnancy: a retrospective study in Hubei province, China","volume":"10","author":"Tan","year":"2023","journal-title":"Front. Med."},{"issue":"18","key":"10.1016\/j.reprotox.2025.108980_bib12","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1056\/NEJMoa1511691","article-title":"Benefits and risks of antiretroviral therapy for perinatal HIV prevention","volume":"375","author":"Fowler","year":"2016","journal-title":"N. Engl. J. Med."},{"issue":"15","key":"10.1016\/j.reprotox.2025.108980_bib13","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1097\/QAD.0000000000002367","article-title":"Association between HIV antiretroviral therapy and preterm birth based on antenatal ultrasound gestational age determination: a comparative analysis","volume":"33","author":"Venkatesh","year":"2019","journal-title":"Aids"},{"issue":"3","key":"10.1016\/j.reprotox.2025.108980_bib14","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1124\/mol.105.012898","article-title":"HIV protease inhibitors activate the unfolded protein response in macrophages: implication for atherosclerosis and cardiovascular disease","volume":"68","author":"Zhou","year":"2005","journal-title":"Mol. Pharmacol."},{"issue":"1","key":"10.1016\/j.reprotox.2025.108980_bib15","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1053\/j.gastro.2009.08.054","article-title":"HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells","volume":"138","author":"Wu","year":"2010","journal-title":"Gastroenterology"},{"issue":"1","key":"10.1016\/j.reprotox.2025.108980_bib16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1093\/infdis\/jiu393","article-title":"HIV protease inhibitor use during pregnancy is associated with decreased progesterone levels, suggesting a potential mechanism contributing to fetal growth restriction","volume":"211","author":"Papp","year":"2015","journal-title":"J. Infect. Dis."},{"issue":"10","key":"10.1016\/j.reprotox.2025.108980_bib17","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1093\/infdis\/jiw004","article-title":"Low prolactin and high 20-alpha-hydroxysteroid dehydrogenase levels contribute to lower progesterone levels in HIV-infected pregnant women exposed to protease inhibitor-based combination antiretroviral therapy","volume":"213","author":"Papp","year":"2016","journal-title":"J. Infect. Dis."},{"issue":"8","key":"10.1016\/j.reprotox.2025.108980_bib18","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1093\/humrep\/deaa151","article-title":"Periconceptional exposure to lopinavir, but not darunavir, impairs decidualization: a potential mechanism leading to poor birth outcomes in HIV-positive pregnancies","volume":"35","author":"Kala","year":"2020","journal-title":"Hum. Reprod."},{"issue":"7","key":"10.1016\/j.reprotox.2025.108980_bib19","first-page":"1231","article-title":"The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro","volume":"28","author":"Pattillo","year":"1968","journal-title":"Cancer Res."},{"issue":"5","key":"10.1016\/j.reprotox.2025.108980_bib20","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1530\/REP-10-0221","article-title":"The choriocarcinoma cell line BeWo: syncytial fusion and expression of syncytium-specific proteins","volume":"140","author":"Orendi","year":"2010","journal-title":"Reproduction"},{"issue":"6","key":"10.1016\/j.reprotox.2025.108980_bib21","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.jnutbio.2003.12.007","article-title":"Caffeine suppresses the expression of the Bcl-2 mRNA in BeWo cell culture and rat placenta","volume":"15","author":"Nomura","year":"2004","journal-title":"J. Nutr. Biochem."},{"issue":"6","key":"10.1016\/j.reprotox.2025.108980_bib22","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.placenta.2003.12.001","article-title":"An analysis using DNA microarray of the time course of gene expression during syncytialization of a human placental cell line (BeWo)","volume":"25","author":"Kudo","year":"2004","journal-title":"Placenta"},{"issue":"24","key":"10.1016\/j.reprotox.2025.108980_bib23","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00034-21","article-title":"Simultaneous or prior activation of intrahepatic type I interferon signaling leads to hepatitis B virus persistence in a mouse model","volume":"95","author":"Zou","year":"2021","journal-title":"J. Virol."},{"issue":"7","key":"10.1016\/j.reprotox.2025.108980_bib24","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1038\/s41374-018-0057-0","article-title":"Principles and approaches for reproducible scoring of tissue stains in research","volume":"98","author":"Meyerholz","year":"2018","journal-title":"Lab. Investig. a J. Tech. Methods Pathol."},{"issue":"11","key":"10.1016\/j.reprotox.2025.108980_bib25","first-page":"1028","article-title":"Liu Jiarong, Ming Fangzhao, Gan Honghui, Cheng Huan, Wu Bo, Liang Ke: incidence and risk factors for low birth weight of infants born to HIV positive women","volume":"23","author":"Xiang Yanni","year":"2017","journal-title":"Chin. J. AIDS STD"},{"issue":"1","key":"10.1016\/j.reprotox.2025.108980_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbcan.2021.188556","article-title":"Tumor suppressor p53: biology, signaling pathways, and therapeutic targeting","volume":"1876","author":"Hernandez Borrero","year":"2021","journal-title":"Biochim. Et. Biophys. Acta Rev. Cancer"},{"issue":"3","key":"10.1016\/j.reprotox.2025.108980_bib27","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.21037\/tcr.2019.12.89","article-title":"The MDM2\/MDMX\/p53 axis in the adaptive stress response","volume":"9","author":"Wang","year":"2020","journal-title":"Transl. Cancer Res."},{"issue":"3","key":"10.1016\/j.reprotox.2025.108980_bib28","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/j.1600-0897.2010.00837.x","article-title":"Placental apoptosis in health and disease","volume":"64","author":"Sharp","year":"2010","journal-title":"Am. J. Reprod. Immunol."},{"key":"10.1016\/j.reprotox.2025.108980_bib29","unstructured":"L. Cobellis, M. De Falco, M. Torella, E. Trabucco, F. Caprio, E. Federico, L. Manente, G. Coppola, V. Laforgia, R. Cassandro, et al: Modulation of Bax expression in physiological and pathological human placentas throughout pregnancy. In vivo 2007, 21(5):777-783.."},{"issue":"1","key":"10.1016\/j.reprotox.2025.108980_bib30","doi-asserted-by":"crossref","first-page":"118","DOI":"10.5603\/FHC.2012.0017","article-title":"Increased apoptotic activity on inflammatory human placentas in spontaneous abortions during the first and second trimester of gestation: a histochemical and immunohistochemical study","volume":"50","author":"Deftereou","year":"2012","journal-title":"Folia Histochem Cytobiol."},{"key":"10.1016\/j.reprotox.2025.108980_bib31","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/B978-0-12-385928-0.00008-0","article-title":"Analysis of nelfinavir-induced endoplasmic reticulum stress","volume":"491","author":"Bruning","year":"2011","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.reprotox.2025.108980_bib32","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.reprotox.2014.10.012","article-title":"Analysis of endoplasmic reticulum stress in placentas of HIV-infected women treated with protease inhibitors","volume":"50","author":"Bruning","year":"2014","journal-title":"Reprod. Toxicol."},{"issue":"1-2","key":"10.1016\/j.reprotox.2025.108980_bib33","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0009-8981(99)00255-7","article-title":"Antioxidant defence in recurrent abortion","volume":"295","author":"Vural","year":"2000","journal-title":"Clin. Chim. Acta Int. J. Clin. Chem."},{"issue":"6","key":"10.1016\/j.reprotox.2025.108980_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2017.301","article-title":"Role of peroxiredoxin2 downregulation in recurrent miscarriage through regulation of trophoblast proliferation and apoptosis","volume":"8","author":"Wu","year":"2017","journal-title":"Cell death Dis."},{"key":"10.1016\/j.reprotox.2025.108980_bib35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.placenta.2020.04.013","article-title":"Region-specific changes in the mRNA and protein expression of LCPUFA biosynthesis enzymes and transporters in the placentae of women with preeclampsia","volume":"95","author":"Rani","year":"2020","journal-title":"Placenta"}],"container-title":["Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623825001510?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623825001510?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T15:36:06Z","timestamp":1752593766000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890623825001510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":35,"alternative-id":["S0890623825001510"],"URL":"https:\/\/doi.org\/10.1016\/j.reprotox.2025.108980","relation":{},"ISSN":["0890-6238"],"issn-type":[{"value":"0890-6238","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Deep RNA analyses for BeWo cells and placentae of HIV positive pregnant women treated with lopinavir\/ritonavir","name":"articletitle","label":"Article Title"},{"value":"Reproductive Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reprotox.2025.108980","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108980"}}