{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:07:11Z","timestamp":1788970031085,"version":"build-2803163510"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001407","name":"India Ministry of Science & Technology Department of Biotechnology","doi-asserted-by":"publisher","award":["BT\/PR46380\/AAQ\/1\/863\/2022"],"award-info":[{"award-number":["BT\/PR46380\/AAQ\/1\/863\/2022"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific & Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Reproductive Toxicology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.reprotox.2026.109330","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T23:37:57Z","timestamp":1787269077000},"page":"109330","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Chlorpyrifos exposure remodels the spermatogenic niche and alters testicular epigenetic dynamics in the absence of overt reproductive toxicity"],"prefix":"10.1016","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1771-4688","authenticated-orcid":false,"given":"Meenakshi","family":"Mansukhani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruqaiya","family":"Tasneem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souvik","family":"Sen Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.reprotox.2026.109330_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph191912209","article-title":"Chlorpyrifos occurrence and toxicological risk assessment: a review","volume":"19","author":"Wo\u0142ejko","year":"2022","journal-title":"Int. J. Environ. Res. Public. Health"},{"key":"10.1016\/j.reprotox.2026.109330_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.142649","article-title":"A comprehensive review on chlorpyrifos toxicity with special reference to endocrine disruption: evidence of mechanisms, exposures and mitigation strategies","volume":"755","author":"Ubaid ur Rahman","year":"2021","journal-title":"Sci. Total. Environ."},{"key":"10.1016\/j.reprotox.2026.109330_bib3","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1289\/ehp.9087245","article-title":"Mechanism of action of organophosphorus and carbamate insecticides","volume":"87","author":"Fukuto","year":"1990","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.reprotox.2026.109330_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2024.109022","article-title":"Chlorpyrifos modulates the mouse gut microbiota and metabolic activity","volume":"192","author":"Nichols","year":"2024","journal-title":"Environ. Int."},{"key":"10.1016\/j.reprotox.2026.109330_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jsbmb.2015.10.010","article-title":"Pesticide chlorpyrifos acts as an endocrine disruptor in adult rats causing changes in mammary gland and hormonal balance","volume":"156","author":"Ventura","year":"2016","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.reprotox.2026.109330_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2025.154296","article-title":"Chronic oral exposure to chlorpyrifos disrupts hepatic epigenetic regulation and induces metabolic dysfunction in mice","volume":"519","author":"Mansukhani","year":"2026","journal-title":"Toxicology"},{"key":"10.1016\/j.reprotox.2026.109330_bib7","doi-asserted-by":"crossref","first-page":"7871","DOI":"10.1073\/pnas.1203396109","article-title":"Brain anomalies in children exposed prenatally to a common organophosphate pesticide","volume":"109","author":"Rauh","year":"2012","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.reprotox.2026.109330_bib8","doi-asserted-by":"crossref","first-page":"20859","DOI":"10.1007\/s11356-017-9617-x","article-title":"Low doses of chlorpyrifos interfere with spermatogenesis of rats through reduction of sex hormones","volume":"24","author":"Peiris","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.reprotox.2026.109330_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2021.152883","article-title":"3,5,6-trichloro-2-pyridinol intensifies the effect of chlorpyrifos on the paracrine function of Sertoli cells by preventing binding of testosterone and the androgen receptor","volume":"460","author":"Gao","year":"2021","journal-title":"Toxicology"},{"key":"10.1016\/j.reprotox.2026.109330_bib10","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1177\/0748233707080908","article-title":"Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat","volume":"23","author":"Joshi","year":"2007","journal-title":"Toxicol. Ind. Health"},{"key":"10.1016\/j.reprotox.2026.109330_bib11","doi-asserted-by":"crossref","first-page":"12455","DOI":"10.1021\/acs.jafc.8b03407","article-title":"Chlorpyrifos induction of testicular-cell apoptosis through generation of reactive oxygen species and phosphorylation of AMPK","volume":"66","author":"Chen","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.reprotox.2026.109330_bib12","doi-asserted-by":"crossref","first-page":"6027","DOI":"10.1080\/07391102.2021.1875885","article-title":"Disruption of androgen receptor signaling by chlorpyrifos (CPF) and its environmental degradation products: a structural insight","volume":"40","author":"Hazarika","year":"2022","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.reprotox.2026.109330_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2020.109785","article-title":"Chlorpyrifos inhibits sperm maturation and induces a decrease in mouse male fertility","volume":"188","author":"Zhang","year":"2020","journal-title":"Environ. Res."},{"key":"10.1016\/j.reprotox.2026.109330_bib14","article-title":"Chlorpyrifos induces male infertility in pigs through ROS and PI3K-AKT pathway","volume":"26","author":"Zhang","year":"2023","journal-title":"IScience"},{"key":"10.1016\/j.reprotox.2026.109330_bib15","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.reprotox.2009.10.003","article-title":"Chlorpyrifos induced reproductive toxicity in male mice","volume":"29","author":"Farag","year":"2010","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.reprotox.2026.109330_bib16","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/andr.12165","article-title":"The Sertoli cell: one hundred fifty years of beauty and plasticity","volume":"4","author":"Fran\u00e7a","year":"2016","journal-title":"Andrology"},{"key":"10.1016\/j.reprotox.2026.109330_bib17","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1530\/rep.1.00358","article-title":"FSH and testosterone signaling in Sertoli cells","volume":"130","author":"Walker","year":"2005","journal-title":"Reproduction"},{"key":"10.1016\/j.reprotox.2026.109330_bib18","doi-asserted-by":"crossref","unstructured":"M.D. Griswold, The central role of Sertoli cells in spermatogenesis, 1998.","DOI":"10.1006\/scdb.1998.0203"},{"key":"10.1016\/j.reprotox.2026.109330_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2024.106065","article-title":"Organophosphate pesticide chlorpyrifos and its metabolite 3,5,6-trichloropyridinol downregulate the expression of genes essential for spermatogenesis in caprine testes","volume":"204","author":"Mansukhani","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.reprotox.2026.109330_bib20","article-title":"Chlorpyrifos induces spermatogenic dysfunction via ferroptosis in Sertoli cells","volume":"12","author":"Fu","year":"2025","journal-title":"Genes. Dis."},{"key":"10.1016\/j.reprotox.2026.109330_bib21","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/toxres\/tfaa050","article-title":"Genome-wide analysis of DNA methylation in testis of male rat exposed to chlorpyrifos","volume":"9","author":"Sai","year":"2020","journal-title":"Toxicol. Res. (Camb. )."},{"key":"10.1016\/j.reprotox.2026.109330_bib22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/em.22242","article-title":"vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo development","volume":"60","author":"Pallotta","year":"2019","journal-title":"Environ. Mol. Mutagen."},{"key":"10.1016\/j.reprotox.2026.109330_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.taap.2020.115275","article-title":"Transgenerational effects of developmental exposure to chlorpyrifos-oxon in zebrafish (Danio rerio)","volume":"408","author":"Schmitt","year":"2020","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.reprotox.2026.109330_bib24","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph191912209","article-title":"Chlorpyrifos Occurrence and Toxicological Risk Assessment: A Review","volume":"19","author":"Wo\u0142ejko","year":"2022","journal-title":"Int. J. Environ. Res. Public. Health"},{"key":"10.1016\/j.reprotox.2026.109330_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2025.154296","article-title":"Chronic oral exposure to chlorpyrifos disrupts hepatic epigenetic regulation and induces metabolic dysfunction in mice","volume":"519","author":"Mansukhani","year":"2026","journal-title":"Toxicology"},{"key":"10.1016\/j.reprotox.2026.109330_bib26","doi-asserted-by":"crossref","DOI":"10.1038\/s41419-026-08606-3","article-title":"Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerability","author":"Tian","year":"2026","journal-title":"Cell. Death & Dis. 2026"},{"key":"10.1016\/j.reprotox.2026.109330_bib27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0022-1759(92)90008-H","article-title":"A microplate assay for the detection of oxidative products using 2\u2032,7\u2032-dichlorofluorescin-diacetate","volume":"156","author":"Rosenkranz","year":"1992","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.reprotox.2026.109330_bib28","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.83129","article-title":"The Slingshot phosphatase 2 is required for acrosome biogenesis during spermatogenesis in mice","volume":"12","author":"Xu","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.reprotox.2026.109330_bib29","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"P, Sacchi, Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate\u2013Phenol\u2013Chloroform Extraction","volume":"162","author":"Chomczyskni","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.reprotox.2026.109330_bib30","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.reprotox.2026.109330_bib31","doi-asserted-by":"crossref","first-page":"6514","DOI":"10.1002\/jcb.26698","article-title":"Sertoli cell specific decline in NOR-1 leads to germ cell apoptosis and reduced fertility","volume":"119","author":"Shukla","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.reprotox.2026.109330_bib32","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1074\/mcp.TIR118.001270","article-title":"Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation","volume":"18","author":"Batth","year":"2019","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.reprotox.2026.109330_bib33","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update)","volume":"50","author":"Sherman","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.reprotox.2026.109330_bib34","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.reprotox.2026.109330_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0294236","article-title":"SRplot: A free online platform for data visualization and graphing","volume":"18","author":"Tang","year":"2023","journal-title":"PLoS. One"},{"key":"10.1016\/j.reprotox.2026.109330_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2021.117010","article-title":"Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides)","volume":"282","author":"Zhao","year":"2021","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.reprotox.2026.109330_bib37","doi-asserted-by":"crossref","first-page":"18566","DOI":"10.1007\/s11356-024-32201-8","article-title":"Grape seed extract prevents chlorpyrifos-induced toxicity in rat liver through the modulation of phase I detoxification pathway","volume":"31","author":"Ghrir","year":"2024","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.reprotox.2026.109330_bib38","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.scitotenv.2015.11.162","article-title":"Hepatotoxicity and nephrotoxicity induced by the chlorpyrifos and chlorpyrifos-methyl metabolite, 3,5,6-trichloro-2-pyridinol, in orally exposed mice","volume":"544","author":"Deng","year":"2016","journal-title":"Sci. Total. Environ."},{"key":"10.1016\/j.reprotox.2026.109330_bib39","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jsbmb.2010.02.032","article-title":"Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos","volume":"120","author":"Viswanath","year":"2010","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.reprotox.2026.109330_bib40","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.toxlet.2025.03.011","article-title":"Dose-dependent effects of chlorpyrifos on liver injury, intestinal dysbiosis, and metabolic perturbations in C57BL\/6J mice","volume":"407","author":"Wei","year":"2025","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.reprotox.2026.109330_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2025.151287","article-title":"Toxicological Impact of Chronic Chlorpyrifos Exposure: DNA Damage and Epigenetic Alterations Induces Neoplastic Transformation of Liver Cells","volume":"746","author":"Balakrishnan","year":"2025","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.reprotox.2026.109330_bib42","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1042\/BST20200026","article-title":"Extracellular matrix stiffness and Wnt\/\u03b2-catenin signaling in physiology and disease","volume":"48","author":"Astudillo","year":"2020","journal-title":"Biochem. Soc. Trans."},{"issue":"1 6","key":"10.1016\/j.reprotox.2026.109330_bib43","article-title":"Extracellular matrix stiffness dictates Wnt expression through integrin pathway","volume":"6","author":"Du","year":"2016","journal-title":"Sci. Rep. 2016"},{"key":"10.1016\/j.reprotox.2026.109330_bib44","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2023.1095894","article-title":"Impact of endocrine disrupting chemicals and pharmaceuticals on Sertoli cell development and functions","volume":"14","author":"Corpuz-Hilsabeck","year":"2023","journal-title":"Front. Endocrinol. (Lausanne)."},{"key":"10.1016\/j.reprotox.2026.109330_bib45","doi-asserted-by":"crossref","unstructured":"C. Mauduit, S. Hamamah, M. Benahmed, Stem cell factor\/c-kit system in spermatogenesis, 1999.","DOI":"10.1093\/humupd\/5.5.535"},{"key":"10.1016\/j.reprotox.2026.109330_bib46","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.tem.2005.07.001","article-title":"Role of connexin-based gap junction channels in testis","volume":"16","author":"Pointis","year":"2005","journal-title":"Trends Endocrinol. Metab."},{"key":"10.1016\/j.reprotox.2026.109330_bib47","doi-asserted-by":"crossref","DOI":"10.1095\/biolreprod.112.105809","article-title":"Regulated wnt\/beta-catenin signaling sustains adult spermatogenesis in mice","volume":"90","author":"Kerr","year":"2014","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.reprotox.2026.109330_bib48","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1095\/biolreprod.109.077446","article-title":"Overactive Beta-Catenin Signaling Causes Testicular Sertoli Cell Tumor Development in the Mouse","volume":"81","author":"Chang","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.reprotox.2026.109330_bib49","doi-asserted-by":"crossref","first-page":"R29","DOI":"10.1530\/REP-15-0463","article-title":"Regulation of blood-testis barrier by actin binding proteins and protein kinases","volume":"151","author":"Li","year":"2015","journal-title":"Reproduction"}],"container-title":["Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623826001735?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890623826001735?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T15:09:27Z","timestamp":1788966567000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890623826001735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":49,"alternative-id":["S0890623826001735"],"URL":"https:\/\/doi.org\/10.1016\/j.reprotox.2026.109330","relation":{},"ISSN":["0890-6238"],"issn-type":[{"value":"0890-6238","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Chlorpyrifos exposure remodels the spermatogenic niche and alters testicular epigenetic dynamics in the absence of overt reproductive toxicity","name":"articletitle","label":"Article Title"},{"value":"Reproductive Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reprotox.2026.109330","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"109330"}}