{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:15:22Z","timestamp":1790396122242,"version":"4.1.0"},"reference-count":55,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":812,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Microbiology"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.resmic.2021.103876","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T11:15:49Z","timestamp":1630322149000},"page":"103876","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterisation of the manganese superoxide dismutase of Enterococcus faecium"],"prefix":"10.1016","volume":"172","author":[{"given":"Valentin","family":"Wasselin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Staerck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Rinc\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"L\u00e9ger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Budin-Verneuil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Hartke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9349-6807","authenticated-orcid":false,"given":"Abdellah","family":"Benachour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1205-2468","authenticated-orcid":false,"given":"Eliette","family":"Riboulet-Bisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.resmic.2021.103876_bib1","series-title":"Enterococci: from commensals to leading causes of drug resistant infection","article-title":"Enterococcus diversity, origins in nature, and gut colonization","author":"Lebreton","year":"2014"},{"key":"10.1016\/j.resmic.2021.103876_bib2","doi-asserted-by":"crossref","DOI":"10.1186\/s12864-018-5043-9","article-title":"Comparative genome analysis reveals key genetic factors associated with probiotic property in Enterococcus faecium strains","volume":"19","author":"Ghattargi","year":"2018","journal-title":"BMC Genom"},{"key":"10.1016\/j.resmic.2021.103876_bib3","article-title":"Pathogenicity of enterococci","volume":"7","author":"Selleck","year":"2019","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.resmic.2021.103876_bib4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4161\/viru.21282","article-title":"Intrinsic and acquired resistance mechanisms in enterococcus","volume":"3","author":"Hollenbeck","year":"2012","journal-title":"Virulence"},{"key":"10.1016\/j.resmic.2021.103876_bib5","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1586\/14787210.2014.956092","article-title":"Mechanisms of antibiotic resistance in enterococci","volume":"12","author":"Miller","year":"2014","journal-title":"Expert Rev Anti Infect Ther"},{"key":"10.1016\/j.resmic.2021.103876_bib6","series-title":"Antibiotic Resistance Threats","year":"2019"},{"key":"10.1016\/j.resmic.2021.103876_bib7","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S1369-5274(99)80033-2","article-title":"Oxidative stress","volume":"2","author":"Storz","year":"1999","journal-title":"Curr Opin Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib8","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1084\/jem.20181886","article-title":"Reactive species and pathogen antioxidant networks during phagocytosis","volume":"216","author":"Piacenza","year":"2019","journal-title":"J\u00a0Exp Med"},{"key":"10.1016\/j.resmic.2021.103876_bib9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1152\/physrev.00044.2005","article-title":"The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology","volume":"87","author":"Bedard","year":"2007","journal-title":"Physiol Rev"},{"key":"10.1016\/j.resmic.2021.103876_bib10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/1462-2920.14445","article-title":"Where in the world do bacteria experience oxidative stress?","volume":"21","author":"Imlay","year":"2019","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib11","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1111\/j.1365-2958.2011.07612.x","article-title":"How does the oxidative burst of macrophages kill bacteria? Still an open question","volume":"80","author":"Slauch","year":"2011","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib12","doi-asserted-by":"crossref","first-page":"22369","DOI":"10.1016\/S0021-9258(18)41538-4","article-title":"The inactivation of Fe-S cluster containing hydro-lyases by superoxide","volume":"268","author":"Flint","year":"1993","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.resmic.2021.103876_bib13","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.bbapap.2004.08.007","article-title":"The oxidative environment and protein damage","volume":"1703","author":"Davies","year":"2005","journal-title":"Biochim Biophys Acta Protein Proteonomics"},{"key":"10.1016\/j.resmic.2021.103876_bib14","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/nrmicro3032","article-title":"The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium","volume":"11","author":"Imlay","year":"2013","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib15","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1099\/mic.0.28922-0","article-title":"Implication of (Mn)superoxide dismutase of Enterococcus faecalis in oxidative stress responses and survival inside macrophages","volume":"152","author":"Verneuil","year":"2006","journal-title":"Microbiology"},{"key":"10.1016\/j.resmic.2021.103876_bib16","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/jac\/dkp369","article-title":"The Enterococcus faecalis superoxide dismutase is essential for its tolerance to vancomycin and penicillin","volume":"64","author":"Bizzini","year":"2009","journal-title":"J\u00a0Antimicrob Chemother"},{"key":"10.1016\/j.resmic.2021.103876_bib17","doi-asserted-by":"crossref","first-page":"2083","DOI":"10.1093\/jac\/dkt157","article-title":"Analysis of the tolerance of pathogenic enterococci and Staphylococcus aureus to cell wall active antibiotics","volume":"68","author":"Ladjouzi","year":"2013","journal-title":"J\u00a0Antimicrob Chemother"},{"key":"10.1016\/j.resmic.2021.103876_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002834","article-title":"AsrR is an oxidative stress sensing regulator modulating Enterococcus faecium opportunistic traits, antimicrobial resistance, and pathogenicity","volume":"8","author":"Lebreton","year":"2012","journal-title":"PLOS Pathog"},{"key":"10.1016\/j.resmic.2021.103876_bib19","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1128\/JB.00259-12","article-title":"Comparative analysis of the first complete Enterococcus faecium genome","volume":"194","author":"Lam","year":"2012","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.resmic.2021.103876_bib20","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1002\/path.1700350614","article-title":"A\u00a0new culture medium for the production of antigenic streptococcal h\u00e6molysin","volume":"35","author":"Todd","year":"1932","journal-title":"J\u00a0Pathol"},{"key":"10.1016\/j.resmic.2021.103876_bib21","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1128\/jb.62.3.293-300.1951","article-title":"Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli","volume":"62","author":"Bertani","year":"1951","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.resmic.2021.103876_bib22","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/j.1462-2920.2010.02355.x","article-title":"A\u00a0genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source","volume":"13","author":"Zhang","year":"2011","journal-title":"Environ Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib23","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1073\/pnas.86.7.2172","article-title":"One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution","volume":"86","author":"Chung","year":"1989","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.resmic.2021.103876_bib24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide dismutase: improved assays and an assay applicable to acrylamide gels","volume":"44","author":"Beauchamp","year":"1971","journal-title":"Anal Biochem"},{"key":"10.1016\/j.resmic.2021.103876_bib25","series-title":"The proteomics protocols handbook","first-page":"571","article-title":"Protein identification and analysis tools on the ExPASy server","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.resmic.2021.103876_bib26","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J\u00a0Mol Biol"},{"key":"10.1016\/j.resmic.2021.103876_bib27","doi-asserted-by":"crossref","first-page":"W459","DOI":"10.1093\/nar\/gky320","article-title":"BUSCA: an integrative web server to predict subcellular localization of proteins","volume":"46","author":"Savojardo","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.resmic.2021.103876_bib28","series-title":"A\u00a0Language and Environment for Statistical Computing","year":"2020"},{"key":"10.1016\/j.resmic.2021.103876_bib29","first-page":"31812","article-title":"Control of Listeria superoxide dismutase by phosphorylation","volume":"281","author":"Archambaud","year":"2006","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.resmic.2021.103876_bib30","doi-asserted-by":"crossref","first-page":"600325","DOI":"10.3389\/fcimb.2020.600325","article-title":"Single-nucleotide RNA maps for the two major nosocomial pathogens Enterococcus faecalis and Enterococcus faecium","volume":"10","author":"Michaux","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib31","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1128\/JCM.38.1.415-418.2000","article-title":"Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci","volume":"38","author":"Poyart","year":"2000","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib32","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/j.cell.2017.04.027","article-title":"Tracing the enterococci from Paleozoic origins to the hospital","volume":"169","author":"Lebreton","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.resmic.2021.103876_bib33","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1046\/j.1365-2958.2001.02638.x","article-title":"Extracellular superoxide production by Enterococcus faecalis requires demethylmenaquinone and is attenuated by functional terminal quinol oxidases","volume":"42","author":"Huycke","year":"2008","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib34","series-title":"The Physiology and Metabolism of Enterococci","author":"Ramsey","year":"2014"},{"key":"10.1016\/j.resmic.2021.103876_bib35","doi-asserted-by":"crossref","first-page":"4512","DOI":"10.1128\/IAI.72.8.4512-4520.2004","article-title":"Cytotoxicity of hydrogen peroxide produced by Enterococcus faecium","volume":"72","author":"Moy","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.resmic.2021.103876_bib36","doi-asserted-by":"crossref","first-page":"40485","DOI":"10.1074\/jbc.M607704200","article-title":"Menadione-induced reactive oxygen species generation via redox cycling promotes apoptosis of murine pancreatic acinar cells","volume":"281","author":"Criddle","year":"2006","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.resmic.2021.103876_bib37","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1099\/mic.0.26353-0","article-title":"Role and regulation of the superoxide dismutases of Staphylococcus aureus","volume":"149","author":"Karavolos","year":"2003","journal-title":"Microbiology"},{"key":"10.1016\/j.resmic.2021.103876_bib38","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.1128\/JB.01496-08","article-title":"Regulation of superoxide dismutase (sod) genes by SarA in Staphylococcus aureus","volume":"191","author":"Ballal","year":"2009","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.resmic.2021.103876_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/abbi.1999.1518","article-title":"Iron and oxidative stress in bacteria","volume":"373","author":"Touati","year":"2000","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/j.resmic.2021.103876_bib40","doi-asserted-by":"crossref","first-page":"5617","DOI":"10.1128\/JB.183.19.5617-5631.2001","article-title":"Global analysis of the general stress response of Bacillus subtilis","volume":"183","author":"Petersohn","year":"2001","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.resmic.2021.103876_bib41","doi-asserted-by":"crossref","first-page":"10846","DOI":"10.1016\/S0021-9258(19)86598-5","article-title":"Paraquat and Escherichia coli. Mechanism of production of extracellular superoxide radical","volume":"254","author":"Hassan","year":"1979","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.resmic.2021.103876_bib42","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1128\/aem.60.7.2360-2366.1994","article-title":"Expression of superoxide dismutase in Listeria monocytogenes","volume":"60","author":"Vasconcelos","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib43","doi-asserted-by":"crossref","first-page":"3732","DOI":"10.1128\/aem.63.9.3732-3735.1997","article-title":"Characterization of superoxide dismutase in Streptococcus thermophilus","volume":"63","author":"Chang","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib44","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1128\/JB.180.14.3697-3703.1998","article-title":"Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis","volume":"180","author":"Inaoka","year":"1998","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.resmic.2021.103876_bib45","doi-asserted-by":"crossref","first-page":"13635","DOI":"10.1073\/pnas.93.24.13635","article-title":"Superoxide accelerates DNA damage by elevating free-iron levels","volume":"93","author":"Keyer","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.resmic.2021.103876_bib46","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/0952-7915(95)80022-0","article-title":"Innate immunity of insects","volume":"7","author":"Hoffmann","year":"1995","journal-title":"Curr Opin Immunol"},{"key":"10.1016\/j.resmic.2021.103876_bib47","doi-asserted-by":"crossref","first-page":"4161","DOI":"10.1128\/IAI.73.7.4161-4170.2005","article-title":"Superoxide production in Galleria mellonella hemocytes: identification of proteins homologous to the NADPH oxidase complex of human neutrophils","volume":"73","author":"Bergin","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.resmic.2021.103876_bib48","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.dci.2006.06.007","article-title":"Translocation of proteins homologous to human neutrophil p47phox and p67phox to the cell membrane in activated hemocytes of Galleria mellonella","volume":"31","author":"Renwick","year":"2007","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.resmic.2021.103876_bib49","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/S0022-1910(99)00223-1","article-title":"Involvement of both granular cells and plasmatocytes in phagocytic reactions in the greater wax moth, Galleria mellonella","volume":"46","author":"Tojo","year":"2000","journal-title":"J\u00a0Insect Physiol"},{"key":"10.1016\/j.resmic.2021.103876_bib50","first-page":"191","article-title":"Galleria mellonella as a model for studying Enterococcus faecium host persistence","volume":"21","author":"Lebreton","year":"2011","journal-title":"J\u00a0Mol Microbiol Biotechnol"},{"key":"10.1016\/j.resmic.2021.103876_bib51","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1365-2958.1993.tb01668.x","article-title":"Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA","volume":"9","author":"Tardat","year":"1993","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib52","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1574-6976.1994.tb00105.x","article-title":"Roles of manganese and iron in the regulation of the biosynthesis of manganese-superoxide dismutase in Escherichia coli","volume":"14","author":"Hassan","year":"1994","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.resmic.2021.103876_bib53","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1046\/j.1365-2958.2002.03113.x","article-title":"Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon","volume":"45","author":"Baichoo","year":"2002","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.resmic.2021.103876_bib54","doi-asserted-by":"crossref","first-page":"4424","DOI":"10.1128\/IAI.72.8.4424-4431.2004","article-title":"Effects of the Enterococcus faecalis hypRgene encoding a new transcriptional regulator on oxidative stress response and intracellular survival within macrophages","volume":"72","author":"Verneuil","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.resmic.2021.103876_bib55","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.femsle.2005.08.043","article-title":"Implication of hypR in the virulence and oxidative stress response of Enterococcus faecalis","volume":"252","author":"Verneuil","year":"2005","journal-title":"FEMS Microbiol Lett"}],"container-title":["Research in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0923250821000905?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0923250821000905?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T05:17:40Z","timestamp":1760332660000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0923250821000905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":55,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["S0923250821000905"],"URL":"https:\/\/doi.org\/10.1016\/j.resmic.2021.103876","relation":{},"ISSN":["0923-2508"],"issn-type":[{"value":"0923-2508","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterisation of the manganese superoxide dismutase of Enterococcus faecium","name":"articletitle","label":"Article Title"},{"value":"Research in Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.resmic.2021.103876","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Institut Pasteur. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"103876"}}