{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:34:32Z","timestamp":1787592872240,"version":"build-2736575974"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015060","name":"Nagasaki University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H03885"],"award-info":[{"award-number":["20H03885"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H03142"],"award-info":[{"award-number":["21H03142"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H04952"],"award-info":[{"award-number":["21H04952"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K19611"],"award-info":[{"award-number":["21K19611"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004721","name":"University of Tokyo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004721","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006004","name":"Tohoku University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006004","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Regenerative Therapy"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.reth.2023.09.017","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T01:44:52Z","timestamp":1696902292000},"page":"536-546","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives"],"prefix":"10.1016","volume":"24","author":[{"given":"Yuki","family":"Ikeda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3090-5073","authenticated-orcid":false,"given":"Shoichiro","family":"Tani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Moriishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiko","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Matsuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Saeki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chizuko","family":"Inui-Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Abe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7852-7775","authenticated-orcid":false,"given":"David W.","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4691-6394","authenticated-orcid":false,"given":"Ung-il","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2245-6853","authenticated-orcid":false,"given":"Hironori","family":"Hojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9577-3249","authenticated-orcid":false,"given":"Yuki","family":"Matsushita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sawase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6874-8539","authenticated-orcid":false,"given":"Shinsuke","family":"Ohba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.reth.2023.09.017_bib1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1002\/dvdy.278","article-title":"Making and shaping endochondral and intramembranous bones","volume":"250","author":"Galea","year":"2021","journal-title":"Dev Dynam"},{"key":"10.1016\/j.reth.2023.09.017_bib2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jdsr.2021.10.001","article-title":"Genome-scale actions of master regulators directing skeletal development","volume":"57","author":"Ohba","year":"2021","journal-title":"Jpn Dent Sci Rev"},{"key":"10.1016\/j.reth.2023.09.017_bib3","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/s12276-020-0482-1","article-title":"Understanding paraxial mesoderm development and sclerotome specification for skeletal repair","volume":"52","author":"Tani","year":"2020","journal-title":"Exp Mol Med"},{"key":"10.1016\/j.reth.2023.09.017_bib4","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/jbmr.4524","article-title":"Differentiation of induced pluripotent stem cells into chondrocytes: methods and applications for disease modeling and drug discovery","volume":"37","author":"De Kinderen","year":"2022","journal-title":"J\u00a0Bone Miner Res"},{"key":"10.1016\/j.reth.2023.09.017_bib5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.semcdb.2021.11.024","article-title":"Developmental principles informing human pluripotent stem cell differentiation to cartilage and bone","volume":"127","author":"Humphreys","year":"2022","journal-title":"Semin Cell Dev Biol"},{"key":"10.1016\/j.reth.2023.09.017_bib6","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.cell.2016.06.011","article-title":"Mapping the pairwise choices leading from pluripotency to human bone, heart, and other mesoderm cell types","volume":"166","author":"Loh","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.reth.2023.09.017_bib7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/s41467-020-20598-y","article-title":"Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis","volume":"12","author":"Wu","year":"2021","journal-title":"Nat Commun"},{"key":"10.1016\/j.reth.2023.09.017_bib8","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/srep00455","article-title":"Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells","volume":"2","author":"Umeda","year":"2012","journal-title":"Sci Rep"},{"key":"10.1016\/j.reth.2023.09.017_bib9","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13287-020-01597-8","article-title":"Prospective isolation of chondroprogenitors from human iPSCs based on cell surface markers identified using a CRISPR-Cas9-generated reporter","volume":"11","author":"Dicks","year":"2020","journal-title":"Stem Cell Res Ther"},{"key":"10.1016\/j.reth.2023.09.017_bib10","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/nbt.3210","article-title":"Generation of articular chondrocytes from human pluripotent stem cells","volume":"33","author":"Craft","year":"2015","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.reth.2023.09.017_bib11","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.celrep.2017.01.040","article-title":"In\u00a0vivo human somitogenesis guides somite development from hPSCs","volume":"18","author":"Xi","year":"2017","journal-title":"Cell Rep"},{"key":"10.1016\/j.reth.2023.09.017_bib12","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1002\/stem.3206","article-title":"Lineage-specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1-RUNX2 association in neural crest-derived osteoprogenitors","volume":"38","author":"Kidwai","year":"2020","journal-title":"Stem Cell"},{"key":"10.1016\/j.reth.2023.09.017_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112276","article-title":"Stem cell-based modeling and single-cell multiomics reveal gene-regulatory mechanisms underlying human skeletal development","volume":"42","author":"Tani","year":"2023","journal-title":"Cell Rep"},{"key":"10.1016\/j.reth.2023.09.017_bib14","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.5966\/sctm.2013-0128","article-title":"A\u00a0site-specific integrated Col2.3GFP reporter identifies osteoblasts within mineralized tissue formed in\u00a0vivo by human embryonic stem cells","volume":"3","author":"Xin","year":"2014","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.reth.2023.09.017_bib15","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.stem.2016.03.014","article-title":"Chemical control of grafted human PSC-derived neurons in a mouse model of Parkinson's disease","volume":"18","author":"Chen","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.reth.2023.09.017_bib16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.reth.2023.09.017_bib17","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1538\/expanim.60.481","article-title":"Effect of three types of mixed\u00a0anesthetic agents alternate to ketamine in mice","volume":"60","author":"Kawai","year":"2011","journal-title":"Exp Anim"},{"key":"10.1016\/j.reth.2023.09.017_bib18","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/j.bone.2009.11.022","article-title":"Hand2 regulates chondrogenesis in\u00a0vitro and in\u00a0vivo","volume":"46","author":"Abe","year":"2010","journal-title":"Bone"},{"key":"10.1016\/j.reth.2023.09.017_bib19","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3727\/000000002783992361","article-title":"Transcripts encoding HAND genes are differentially expressed and regulated by BMP4 and GDNF in developing avian gut","volume":"10","author":"Wu","year":"2002","journal-title":"Gene Expr"},{"key":"10.1016\/j.reth.2023.09.017_bib20","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s00441-014-1931-8","article-title":"Kruppel-like factor 4 expression in osteoblasts represses osteoblast-dependent osteoclast maturation","volume":"358","author":"Fujikawa","year":"2014","journal-title":"Cell Tissue Res"},{"key":"10.1016\/j.reth.2023.09.017_bib21","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.reth.2023.09.017_bib22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.reth.2023.09.017_bib23","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.reth.2023.09.017_bib24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"10.1016\/j.reth.2023.09.017_bib25","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1186\/s12859-018-2486-6","article-title":"iDEP: an integrated web application for differential expression and pathway analysis of RNA-Seq data","volume":"19","author":"Ge","year":"2018","journal-title":"BMC Bioinf"},{"key":"10.1016\/j.reth.2023.09.017_bib26","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"10.1016\/j.reth.2023.09.017_bib27","doi-asserted-by":"crossref","first-page":"5530","DOI":"10.1016\/j.biomaterials.2013.03.089","article-title":"Bone healing by sterilizable calcium phosphate tetrapods eluting osteogenic molecules","volume":"34","author":"Maeda","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.reth.2023.09.017_bib28","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.stemcr.2014.04.016","article-title":"Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions","volume":"2","author":"Kanke","year":"2014","journal-title":"Stem Cell Rep"},{"key":"10.1016\/j.reth.2023.09.017_bib29","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1602875","article-title":"Three-dimensional system enabling the maintenance and directed differentiation of pluripotent stem cells under defined conditions","volume":"3","author":"Zujur","year":"2017","journal-title":"Sci Adv"},{"key":"10.1016\/j.reth.2023.09.017_bib30","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.reth.2019.12.010","article-title":"Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers","volume":"14","author":"Zujur","year":"2020","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2023.09.017_bib31","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.celrep.2015.06.013","article-title":"Distinct transcriptional programs underlie Sox9 regulation of the mammalian chondrocyte","volume":"12","author":"Ohba","year":"2015","journal-title":"Cell Rep"},{"key":"10.1016\/j.reth.2023.09.017_bib32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/s41586-018-0554-8","article-title":"Discovery of a periosteal stem cell mediating intramembranous bone formation","volume":"562","author":"Debnath","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.reth.2023.09.017_bib33","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1002\/dvdy.23992","article-title":"Angiogenesis and intramembranous osteogenesis","volume":"242","author":"Percival","year":"2013","journal-title":"Dev Dynam"},{"key":"10.1016\/j.reth.2023.09.017_bib34","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21186665","article-title":"Hedgehog signaling in skeletal development: roles of Indian hedgehog and the mode of its action","volume":"21","author":"Ohba","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.reth.2023.09.017_bib35","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1101\/gad.13.16.2072","article-title":"Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation","volume":"13","author":"St-Jacques","year":"1999","journal-title":"Genes Dev"},{"key":"10.1016\/j.reth.2023.09.017_bib36","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1101\/gad.13.2.225","article-title":"Sonic hedgehog promotes somitic chondrogenesis by altering the cellular response to BMP signaling","volume":"13","author":"Murtaugh","year":"1999","journal-title":"Genes Dev"},{"key":"10.1016\/j.reth.2023.09.017_bib37","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1101\/gad.1008002","article-title":"Shh establishes an Nkx3.2\/Sox9 autoregulatory loop that is maintained by BMP signals to induce somitic chondrogenesis","volume":"16","author":"Zeng","year":"2002","journal-title":"Genes Dev"},{"key":"10.1016\/j.reth.2023.09.017_bib38","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/j.bbrc.2007.03.173","article-title":"A\u00a0novel osteogenic helioxanthin-derivative acts in a BMP-dependent manner","volume":"357","author":"Ohba","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.reth.2023.09.017_bib39","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1007\/s00774-020-01176-0","article-title":"Sclerostin: from bench to bedside","volume":"39","author":"Tanaka","year":"2021","journal-title":"J\u00a0Bone Miner Metabol"},{"key":"10.1016\/j.reth.2023.09.017_bib40","doi-asserted-by":"crossref","first-page":"6271","DOI":"10.1038\/s41467-021-26571-7","article-title":"Control of osteocyte dendrite formation by Sp7 and its target gene osteocrin","volume":"12","author":"Wang","year":"2021","journal-title":"Nat Commun"},{"key":"10.1016\/j.reth.2023.09.017_bib41","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1210\/er.2017-00220","article-title":"X-linked hypophosphatemia and FGF23-related hypophosphatemic diseases: prospect for new treatment","volume":"39","author":"Kinoshita","year":"2018","journal-title":"Endocr Rev"},{"key":"10.1016\/j.reth.2023.09.017_bib42","doi-asserted-by":"crossref","first-page":"E38","DOI":"10.1152\/ajpendo.00008.2006","article-title":"Pathogenic role of Fgf23 in hyp mice","volume":"291","author":"Liu","year":"2006","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"10.1016\/j.reth.2023.09.017_bib43","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1750-1172-2-42","article-title":"Mowat-Wilson syndrome","volume":"2","author":"Garavelli","year":"2007","journal-title":"Orphanet J Rare Dis"},{"key":"10.1016\/j.reth.2023.09.017_bib44","first-page":"575","article-title":"Relations and interactions between cranial mesoderm and neural crest populations","volume":"207","author":"Noden","year":"2005","journal-title":"J\u00a0Anat"},{"key":"10.1016\/j.reth.2023.09.017_bib45","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.devcel.2016.04.002","article-title":"Sp7\/Osterix is restricted to bone-forming vertebrates where it acts as a dlx Co-factor in osteoblast specification","volume":"37","author":"Hojo","year":"2016","journal-title":"Dev Cell"}],"container-title":["Regenerative Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320423000998?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320423000998?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T17:45:36Z","timestamp":1762451136000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352320423000998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":45,"alternative-id":["S2352320423000998"],"URL":"https:\/\/doi.org\/10.1016\/j.reth.2023.09.017","relation":{},"ISSN":["2352-3204"],"issn-type":[{"value":"2352-3204","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives","name":"articletitle","label":"Article Title"},{"value":"Regenerative Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reth.2023.09.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}