{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:07:20Z","timestamp":1788970040826,"version":"build-2803163510"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021214","name":"Farhangian University","doi-asserted-by":"publisher","award":["25,26"],"award-info":[{"award-number":["25,26"]}],"id":[{"id":"10.13039\/501100021214","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 24K13435"],"award-info":[{"award-number":["JP 24K13435"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Regenerative Therapy"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.reth.2025.10.019","type":"journal-article","created":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T01:45:51Z","timestamp":1761961551000},"page":"991-998","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Microbiome-based profiles of airborne bacteria to support microbial risk assessment in cleanroom environments"],"prefix":"10.1016","volume":"30","author":[{"given":"Mitsuru","family":"Mizuno","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5993-4356","authenticated-orcid":false,"given":"Yusuke","family":"Ogata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuto","family":"Nishihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwako","family":"Nishio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6952-895X","authenticated-orcid":false,"given":"Hisako","family":"Katano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6331-722X","authenticated-orcid":false,"given":"Ichiro","family":"Sekiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.reth.2025.10.019_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0318006","article-title":"Changeover method for biosafety cabinets using ozone gas","volume":"20","author":"Mizuno","year":"2025","journal-title":"PLoS One"},{"key":"10.1016\/j.reth.2025.10.019_bib2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.reth.2024.01.001","article-title":"Operator-derived particles and falling bacteria in biosafety cabinets","volume":"25","author":"Mizuno","year":"2024","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.reth.2024.11.020","article-title":"Cleaning methods for biosafety cabinet to eliminate residual mycoplasmas, viruses, and endotoxins after changeover","volume":"28","author":"Mizuno","year":"2025","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib4","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.reth.2025.03.020","article-title":"Operator stress factors and cell contamination risks in cell processing facilities: an online survey-based analysis","volume":"29","author":"Sugahara","year":"2025","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib5","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/j.reth.2024.11.002","article-title":"Decreasing electricity costs of clean room for cell products during non-operation","volume":"26","author":"Mizuno","year":"2024","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.reth.2023.07.002","article-title":"Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators","volume":"24","author":"Mizuno","year":"2023","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.reth.2023.01.009","article-title":"Effect of disinfectants and manual wiping for processing the cell product changeover in a biosafety cabinet","volume":"22","author":"Mizuno","year":"2023","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib8","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.reth.2022.10.004","article-title":"Stress and motivation of cell processing operators: a pilot study of an online questionnaire survey","volume":"21","author":"Mizuno","year":"2022","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.reth.2024.07.002","article-title":"Evaluation of cleaning methods for change-over after the processing of cell products to avoid cross-contamination risk","volume":"26","author":"Mizuno","year":"2024","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0236600","article-title":"The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution","volume":"15","author":"Mizuno","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.reth.2025.10.019_bib11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.reth.2016.06.004","article-title":"Experience of contamination during autologous cell manufacturing in cell processing facility under the Japanese medical practitioners act and the Medical Care Act","volume":"5","author":"Mizutani","year":"2016","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib12","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.reth.2024.03.028","article-title":"Microbes identified from monitoring cell manipulations in 5-year life of the Cell Factory G. Gaslini","volume":"27","author":"Morandi","year":"2024","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2024.109170","article-title":"Noninvasive total counting of cultured cells using a home-use scanner with a pattern sheet","volume":"27","author":"Mizuno","year":"2024","journal-title":"iScience"},{"key":"10.1016\/j.reth.2025.10.019_bib14","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s13287-022-02850-y","article-title":"Cell membrane fluidity and ROS resistance define DMSO tolerance of cryopreserved synovial MSCs and HUVECs","volume":"13","author":"Mizuno","year":"2022","journal-title":"Stem Cell Res Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib15","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.3109\/14653249.2012.698728","article-title":"Real-time monitoring of non-viable airborne particles correlates with airborne colonies and represents an acceptable surrogate for daily assessment of cell-processing cleanroom performance","volume":"14","author":"Raval","year":"2012","journal-title":"Cytotherapy"},{"key":"10.1016\/j.reth.2025.10.019_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejps.2019.104973","article-title":"Culture independent approach reveals domination of human-oriented microbes in a pharmaceutical manufacturing facility","volume":"137","author":"Kawai","year":"2019","journal-title":"Eur J Pharmaceut Sci"},{"key":"10.1016\/j.reth.2025.10.019_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01257-24","article-title":"The environmental microbial retrieving assessment of cell-processing facilities for cell therapy in a hospital laboratory","volume":"12","author":"Lian","year":"2024","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.reth.2025.10.019_bib18","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.reth.2025.10.019_bib19","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nrmicro.2017.157","article-title":"The human skin microbiome","volume":"16","author":"Byrd","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib20","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1038\/s41579-024-01077-3","article-title":"The indoors microbiome and human health","volume":"22","author":"Gilbert","year":"2024","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib21","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/srep00525","article-title":"Global dispersion of bacterial cells on Asian dust","volume":"2","author":"Yamaguchi","year":"2012","journal-title":"Sci Rep"},{"key":"10.1016\/j.reth.2025.10.019_bib22","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1099\/ijsem.0.003376","article-title":"Paracoccus haematequi sp. Nov., isolated from horse blood","volume":"69","author":"K\u00e4mpfer","year":"2019","journal-title":"Int J Syst Evol Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib23","doi-asserted-by":"crossref","DOI":"10.1099\/ijsem.0.005838","article-title":"Whole-genome sequencing of Paracoccus species isolated from the healthy human eye and description of paracoccus shanxieyensis sp","volume":"73","author":"Dong","year":"2023","journal-title":"Int J Syst Evol Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib24","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1086\/420824","article-title":"Clinical significance of Roseomonas species isolated from catheter and blood samples: analysis of 36 cases in patients with cancer","volume":"38","author":"D\u00e9","year":"2004","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.reth.2025.10.019_bib25","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1134\/S0026261713020070","article-title":"Diversity of bacteria of the family Halomonadaceae at the mining area of the Verkhnekamsk salt deposit","volume":"82","author":"Korsakova","year":"2013","journal-title":"Microbiology"},{"key":"10.1016\/j.reth.2025.10.019_bib26","doi-asserted-by":"crossref","first-page":"729","DOI":"10.3389\/fmicb.2016.00729","article-title":"Osmotic stress confers enhanced cell integrity to hydrostatic pressure but impairs growth in Alcanivorax borkumensis SK2","volume":"7","author":"Scoma","year":"2016","journal-title":"Front Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib27","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.reth.2022.12.003","article-title":"Cross-contamination risk and decontamination during changeover after cell-product processing","volume":"22","author":"Mizuno","year":"2023","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2025.10.019_bib28","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s40168-015-0116-3","article-title":"Microbiomes of the dust particles collected from the International space station and spacecraft assembly facilities","volume":"3","author":"Checinska","year":"2015","journal-title":"Microbiome"},{"key":"10.1016\/j.reth.2025.10.019_bib29","doi-asserted-by":"crossref","first-page":"6760","DOI":"10.1128\/AEM.02244-14","article-title":"Indoor-air microbiome in an urban subway network: diversity and dynamics","volume":"80","author":"Leung","year":"2014","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.reth.2025.10.019_bib30","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00931-24","article-title":"rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA","volume":"12","author":"Won","year":"2024","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.reth.2025.10.019_bib31","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1038\/s41598-017-02516-3","article-title":"Comparison of DNA-, PMA-, and RNA-Based 16S rRNA Illumina sequencing for detection of live bacteria in water","volume":"7","author":"Li","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.reth.2025.10.019_bib32","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s12915-014-0087-z","article-title":"Reagent and laboratory contamination can critically impact sequence-based microbiome analyses","volume":"12","author":"Salter","year":"2014","journal-title":"BMC Biol"},{"key":"10.1016\/j.reth.2025.10.019_bib33","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.mimet.2007.12.011","article-title":"Reagent decontamination to eliminate false-positives in Escherichia coli qPCR","volume":"72","author":"Silkie","year":"2008","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.reth.2025.10.019_bib34","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/apm.13010","article-title":"The environmental occurrence of Pseudomonas aeruginosa","volume":"128","author":"Crone","year":"2020","journal-title":"APMIS"},{"key":"10.1016\/j.reth.2025.10.019_bib35","doi-asserted-by":"crossref","DOI":"10.1099\/mic.0.001335","article-title":"Genetic and environmental determinants of surface adaptations in Pseudomonas aeruginosa","volume":"169","author":"Ambreetha","year":"2023","journal-title":"Microbiol"}],"container-title":["Regenerative Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320425002160?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320425002160?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:09:17Z","timestamp":1773274157000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352320425002160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":35,"alternative-id":["S2352320425002160"],"URL":"https:\/\/doi.org\/10.1016\/j.reth.2025.10.019","relation":{},"ISSN":["2352-3204"],"issn-type":[{"value":"2352-3204","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microbiome-based profiles of airborne bacteria to support microbial risk assessment in cleanroom environments","name":"articletitle","label":"Article Title"},{"value":"Regenerative Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reth.2025.10.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine.","name":"copyright","label":"Copyright"}]}}