{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:46:48Z","timestamp":1782733608233,"version":"3.54.5"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007962","name":"Jikei University School of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007962","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03806"],"award-info":[{"award-number":["19H03806"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25861597"],"award-info":[{"award-number":["25861597"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K18785"],"award-info":[{"award-number":["19K18785"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K09357"],"award-info":[{"award-number":["18K09357"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K11198"],"award-info":[{"award-number":["16K11198"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K16907"],"award-info":[{"award-number":["18K16907"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["19bk0104086"],"award-info":[{"award-number":["19bk0104086"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["18bk0104051"],"award-info":[{"award-number":["18bk0104051"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Regenerative Therapy"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.reth.2026.101111","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:48:04Z","timestamp":1775522884000},"page":"101111","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Long-term outcomes of middle ear regenerative therapy using cell sheet transplantation combined with tympanoplasty"],"prefix":"10.1016","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6509-929X","authenticated-orcid":false,"given":"Kazuhisa","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsunetaro","family":"Morino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-5307","authenticated-orcid":false,"given":"Akira","family":"Baba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Kasai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3042-0719","authenticated-orcid":false,"given":"Yuki","family":"Takizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuki","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Yamato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-2110","authenticated-orcid":false,"given":"Hiromi","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.reth.2026.101111_bib1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1097\/MAO.0000000000004757","article-title":"Investigation of mastoid cavity aeration following canal wall up tympanoplasty with mastoidectomy in middle ear cholesteatoma","volume":"47","author":"Yamamoto","year":"2025","journal-title":"Otol Neurotol"},{"key":"10.1016\/j.reth.2026.101111_bib2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1080\/00016489950181972","article-title":"Gas exchange function through the middle ear mucosa in piglets: comparative study of normal and inflamed ears","volume":"119","author":"Yamamoto","year":"1999","journal-title":"Acta Otolaryngol"},{"key":"10.1016\/j.reth.2026.101111_bib3","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/s41536-017-0010-7","article-title":"Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation","volume":"2","author":"Yamamoto","year":"2017","journal-title":"npj Regen Med"},{"key":"10.1016\/j.reth.2026.101111_bib4","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1002\/jbm.820271005","article-title":"A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly (N-isopropylacrylamide)","volume":"27","author":"Okano","year":"1993","journal-title":"J Biomed Mater Res"},{"key":"10.1016\/j.reth.2026.101111_bib5","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1089\/10763270152436517","article-title":"Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature","volume":"7","author":"Yamato","year":"2001","journal-title":"Tissue Eng"},{"key":"10.1016\/j.reth.2026.101111_bib6","doi-asserted-by":"crossref","first-page":"156","DOI":"10.2492\/inflammregen.27.156","article-title":"Cell sheet engineering for regenerative medicine: from the viewpoint of inflammation","volume":"27","author":"Yamato","year":"2007","journal-title":"Inflamm Regen"},{"key":"10.1016\/j.reth.2026.101111_bib7","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1056\/NEJMoa040455","article-title":"Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium","volume":"351","author":"Nishida","year":"2004","journal-title":"N Engl J Med"},{"key":"10.1016\/j.reth.2026.101111_bib8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1053\/j.gastro.2012.04.050","article-title":"Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets","volume":"143","author":"Ohki","year":"2012","journal-title":"Gastroenterology"},{"key":"10.1016\/j.reth.2026.101111_bib9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s00595-011-0106-4","article-title":"Tissue engineered myoblast sheets improved cardiac function sufficiently to discontinue LVAS in a patient with DCM: report of a case","volume":"42","author":"Sawa","year":"2012","journal-title":"Surg Today"},{"key":"10.1016\/j.reth.2026.101111_bib10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41536-017-0031-2","article-title":"Bio-artificial pleura using an autologous dermal fibroblast sheet","volume":"2","author":"Kanzaki","year":"2017","journal-title":"npj Regen Med"},{"key":"10.1016\/j.reth.2026.101111_bib11","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.reth.2025.06.001","article-title":"Mid-term outcomes of a bio-artificial pleura composed of autologous dermal fibroblasts used to close intraoperative pulmonary air leaks caused by intraoperative pleural injury: a case report","volume":"30","author":"Kanzaki","year":"2025","journal-title":"Regen Ther"},{"key":"10.1016\/j.reth.2026.101111_bib12","article-title":"Long-term outcomes of regenerative treatment by endoscopic oral mucosal epithelial cell sheet transplantation for the prevention of esophageal stricture after endoscopic resection","volume":"19","author":"Ohki","year":"2023","journal-title":"J Immunol Regen Med"},{"issue":"1","key":"10.1016\/j.reth.2026.101111_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5152\/iao.2017.3363","article-title":"EAONO\/JOS joint consensus statements on the definitions, classification and staging of middle ear cholesteatoma","volume":"13","author":"Yung","year":"2017","journal-title":"Int Adv Otol"},{"issue":"2","key":"10.1016\/j.reth.2026.101111_bib14","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.anl.2016.06.012","article-title":"Staging and classification criteria for middle ear cholesteatoma proposed by the Japan otological society","volume":"44","author":"Tono","year":"2017","journal-title":"Auris Nasus Larynx"},{"key":"10.1016\/j.reth.2026.101111_bib15","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.reth.2019.05.001","article-title":"Analysis of human nasal mucosal cell sheets fabricated using transported tissue and blood specimens","volume":"11","author":"Kasai","year":"2019","journal-title":"Regen Ther"},{"issue":"3","key":"10.1016\/j.reth.2026.101111_bib16","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0194-5998(95)70103-6","article-title":"Committee on hearing and equilibrium guidelines for the evaluation of results of treatment of conductive hearing loss. American academy of otolaryngology-head and neck surgery foundation","volume":"113","year":"1995","journal-title":"Inc Otolaryngol Head Neck Surg"},{"issue":"6","key":"10.1016\/j.reth.2026.101111_bib17","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.anl.2020.06.006","article-title":"The impact of middle ear aeration on surgical outcome after intact canal wall tympanoplasty for cholesteatoma","volume":"47","author":"Motegi","year":"2020","journal-title":"Auris Nasus Larynx"},{"issue":"Suppl. 414","key":"10.1016\/j.reth.2026.101111_bib18","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3109\/00016488409122876","article-title":"Ultrastructure of the middle ear mucosa","author":"Hentzer","year":"1984","journal-title":"Acta Otolaryngol"},{"issue":"4","key":"10.1016\/j.reth.2026.101111_bib19","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1002\/term.2012","article-title":"Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery","volume":"11","author":"Hama","year":"2017","journal-title":"J Tissue Eng Regen Med"},{"key":"10.1016\/j.reth.2026.101111_bib20","first-page":"178","article-title":"The partially implantable middle ear implant, case reports","volume":"37","author":"Suzuki","year":"1987","journal-title":"Adv Otorhinolaryngol"},{"key":"10.1016\/j.reth.2026.101111_bib21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/01.mlg.0000154728.23657.3a","article-title":"Regeneration of mastoid air cells in clinical applications by in situ tissue engineering","volume":"115","author":"Kanemaru","year":"2005","journal-title":"Laryngoscope"}],"container-title":["Regenerative Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320426000520?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352320426000520?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:21:50Z","timestamp":1782732110000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352320426000520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":21,"alternative-id":["S2352320426000520"],"URL":"https:\/\/doi.org\/10.1016\/j.reth.2026.101111","relation":{},"ISSN":["2352-3204"],"issn-type":[{"value":"2352-3204","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Long-term outcomes of middle ear regenerative therapy using cell sheet transplantation combined with tympanoplasty","name":"articletitle","label":"Article Title"},{"value":"Regenerative Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.reth.2026.101111","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine.","name":"copyright","label":"Copyright"}],"article-number":"101111"}}