{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:50:14Z","timestamp":1768017014688,"version":"3.49.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.rvsc.2007.02.006","type":"journal-article","created":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T07:33:03Z","timestamp":1176881583000},"page":"340-346","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis"],"prefix":"10.1016","volume":"83","author":[{"given":"Stella Maria","family":"Barrouin-Melo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Padilla","family":"Poester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Borges","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano Costa","family":"de Alc\u00e2ntara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Henrique Palis","family":"Aguiar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana L\u00facia","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Eduard","family":"Schaer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Meyer","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songel\u00ed Menezes","family":"Freire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2007.02.006_bib1","series-title":"Techniques for the Brucellosis Laboratory","author":"Alton","year":"1988"},{"key":"10.1016\/j.rvsc.2007.02.006_bib2","series-title":"Statistical Methods in Medical Research","author":"Armitage","year":"1994"},{"key":"10.1016\/j.rvsc.2007.02.006_bib3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0378-1135(94)90142-2","article-title":"Brucella abortus cytoplasmic proteins used as antigens in an ELISA potentially useful for the diagnosis of canine brucellosis","volume":"41","author":"Baldi","year":"1994","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib4","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0378-1135(97)00134-X","article-title":"Diagnosis of canine brucellosis by detection of serum antibodies against an 18kDa cytoplasmic protein of Brucella spp","volume":"51","author":"Baldi","year":"1997","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib5","first-page":"125","article-title":"Brucelloses canines","volume":"25","author":"Berthelot","year":"1993","journal-title":"Le Point Veterinaire"},{"key":"10.1016\/j.rvsc.2007.02.006_bib6","unstructured":"Brasil, 2001. Departamento de Defesa Animal. Informa\u00e7\u00f5es sobre o PNCBET. Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento, Bras\u00edlia, Brasil. <http:www.agricultura.gov.br\/sda\/dda\/programa.htm>."},{"key":"10.1016\/j.rvsc.2007.02.006_bib7","first-page":"1126","article-title":"Abortion in 200 Beagles","volume":"149","author":"Carmichael","year":"1966","journal-title":"JAVMA"},{"key":"10.1016\/j.rvsc.2007.02.006_bib8","first-page":"579","article-title":"Characterization of a newly recognized species of Brucella responsible for infectious canine abortions","volume":"58","author":"Carmichael","year":"1968","journal-title":"Cornell Vet."},{"key":"10.1016\/j.rvsc.2007.02.006_bib9","first-page":"63","article-title":"Transmission of Brucella canis by contact exposure","volume":"78","author":"Carmichael","year":"1988","journal-title":"Cornell Vet."},{"key":"10.1016\/j.rvsc.2007.02.006_bib10","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1096-2867(96)80028-4","article-title":"Canine brucellosis: a diagnostician\u2019s dilemma","volume":"11","author":"Carmichael","year":"1996","journal-title":"Semin. Vet. Med. Surg. (Small Animal)"},{"key":"10.1016\/j.rvsc.2007.02.006_bib11","series-title":"Manual of Clinical Laboratory Immunology","first-page":"20","article-title":"Enzyme-linked immunoassays","author":"Carpenter","year":"1997"},{"key":"10.1016\/j.rvsc.2007.02.006_bib12","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.1128\/iai.58.12.3980-3987.1990","article-title":"Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay","volume":"58","author":"Cloeckaert","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1016\/j.rvsc.2007.02.006_bib13","first-page":"65","article-title":"Serological diagnosis of brucellosis caused by Brucella canis","volume":"26","author":"Ebani","year":"2003","journal-title":"New Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib14","unstructured":"Ferreira, W., \u00c1vila, S.L.M., 1996. Diagn\u00f3stico laboratorial das principais doen\u00e7as infecciosas e auto-imunes. Guanabara Koogan, Rio de Janeiro; p. 302."},{"key":"10.1016\/j.rvsc.2007.02.006_bib15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0022-1759(98)00170-7","article-title":"A statistically defined endpoint titer determination method for immunoassays","volume":"221","author":"Frey","year":"1998","journal-title":"J. Immunol. Method"},{"key":"10.1016\/j.rvsc.2007.02.006_bib16","first-page":"35","article-title":"Isolamento de Brucella canis em Minas Gerais Brasil","volume":"29","author":"Godoy","year":"1977","journal-title":"Arq. Esc. Vet. Univ. Fed. Minas Gerais"},{"key":"10.1016\/j.rvsc.2007.02.006_bib17","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1099\/00222615-48-9-833","article-title":"The 18-kDa cytoplasmic protein of Brucella species \u2013 an antigen useful for diagnosis \u2013 is a lumazine synthase","volume":"48","author":"Goldbaun","year":"1999","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib18","first-page":"555","article-title":"Selection and interpretation of diagnostic tests and procedures","volume":"94","author":"Griner","year":"1981","journal-title":"Ann. Intern. Med."},{"key":"10.1016\/j.rvsc.2007.02.006_bib19","first-page":"763","article-title":"Clinical signs and diagnosis of Brucella canis infection","volume":"14","author":"Johnson","year":"1992","journal-title":"Comp. Cont. Educ."},{"key":"10.1016\/j.rvsc.2007.02.006_bib20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1590\/S1517-83822004000100027","article-title":"Brucella spp. isolation from dogs from commercial breeding kennels in S\u00e3o Paulo State, Brazil","volume":"35","author":"Keid","year":"2004","journal-title":"Braz. J. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-1135(97)00196-X","article-title":"Characterization of an immuno-dominant antigen in Brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay","volume":"59","author":"Kittelberger","year":"1998","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib22","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1128\/cdli.4.5.556-564.1997","article-title":"Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay","volume":"4","author":"Letesson","year":"1997","journal-title":"Clin. Diag. Lab. Immunol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib23","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.vetmic.2004.10.020","article-title":"Use of Brucella canis antigen for detection of ovine serum antibodies against Brucella ovis","volume":"105","author":"Lopez","year":"2005","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib24","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.rvsc.2007.02.006_bib25","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1099\/0022-1317-51-8-656","article-title":"Sensitivity and specificity of an indirect enzyme-linked immunoassay for the diagnosis of Brucella canis infection in dogs","volume":"51","author":"Lucero","year":"2002","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib26","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1099\/jmm.0.45927-0","article-title":"Diagnosis of human brucellosis caused by Brucella canis","volume":"54","author":"Lucero","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib27","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.2460\/ajvr.1993.54.07.1043","article-title":"Use of indirect enzyme-linked assay with hot saline solution extracts of a variant (M-) strain of Brucella canis for diagnosis of brucellosis in dogs","volume":"54","author":"Mateu-de-Antonio","year":"1993","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.rvsc.2007.02.006_bib28","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.2460\/javma.1970.156.12.1737","article-title":"Epizootiology, diagnosis and control of Brucella canis","volume":"156","author":"Moore","year":"1970","journal-title":"JAVMA"},{"key":"10.1016\/j.rvsc.2007.02.006_bib29","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1128\/am.23.5.894-902.1972","article-title":"Studies of antigens for complement fixation and gel diffusion tests in the diagnosis of infections caused by Brucella ovis and other Brucellae","volume":"23","author":"Myers","year":"1972","journal-title":"Appl. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib30","first-page":"258","article-title":"Serological and bacteriological detection of Brucella canis infection of stray dogs in Moreno, Argentina","volume":"70","author":"Myers","year":"1980","journal-title":"Cornell Vet."},{"key":"10.1016\/j.rvsc.2007.02.006_bib31","series-title":"M\u00e9thodes statistiques \u00e0 l\u2019usage des m\u00e9decins et des biologistes","author":"Schwartz","year":"1987"},{"key":"10.1016\/j.rvsc.2007.02.006_bib32","first-page":"8","article-title":"Inqu\u00e9rito sorol\u00f3gico para brucelose canina por Brucella canis na regi\u00e3o do planalto catarinense \u2013 Brasil","volume":"12","author":"Schlemper","year":"1990","journal-title":"Rev. Bras. Med. Vet."},{"key":"10.1016\/j.rvsc.2007.02.006_bib33","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1128\/jcm.27.5.837-842.1989","article-title":"Purification of Brucella canis cell wall antigen using immunosorbent assay for specific diagnosis of canine brucellosis","volume":"27","author":"Serikawa","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib34","doi-asserted-by":"crossref","first-page":"914","DOI":"10.2460\/javma.1996.209.05.914","article-title":"Proficiency testing of selected antigen and antibody tests for use in dogs and cats","volume":"209","author":"Troy","year":"1996","journal-title":"JAVMA"},{"key":"10.1016\/j.rvsc.2007.02.006_bib35","first-page":"305","article-title":"Brucelose canina: Relato de caso","volume":"26","author":"Vargas","year":"1996","journal-title":"Ci\u00eancia Veterin\u00e1ria"},{"key":"10.1016\/j.rvsc.2007.02.006_bib36","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.anireprosci.2004.05.005","article-title":"Canine brucellosis","volume":"82\u201383","author":"Wanke","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.rvsc.2007.02.006_bib37","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0378-1135(02)00152-9","article-title":"Comparative performance of tests using cytosolic or outer membrane antigens of Brucella for the serodiagnosis of canine brucellosis","volume":"88","author":"Wanke","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib38","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.theriogenology.2006.01.034","article-title":"Use of enrofloxacina in the treatment of canine brucellosis in a dog kennel (clinical trial)","volume":"66","author":"Wanke","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2007.02.006_bib39","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/jb.61.2.239-240.1951","article-title":"Differentiation of smooth and non-smooth colonies of brucellae","volume":"61","author":"White","year":"1951","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.rvsc.2007.02.006_bib40","unstructured":"Zwirner, N.W., 1996. ELISA. In: Margni, R.A. Imunolog\u00eda e Imunoqu\u00edmica. fifth ed. Editorial M\u00e9dica Panamericana., Buenos Aires; pp. 798\u2013820."}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528807000501?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528807000501?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T03:40:26Z","timestamp":1758512426000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0034528807000501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S0034528807000501"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2007.02.006","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2007.02.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}