{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T07:17:22Z","timestamp":1794035842009,"version":"4.8.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Belgian Technical Cooperation"},{"name":"SPW-DGARNE","award":["D31-1284"],"award-info":[{"award-number":["D31-1284"]}]},{"DOI":"10.13039\/501100002661","name":"FNRS","doi-asserted-by":"crossref","award":["1.5155.12"],"award-info":[{"award-number":["1.5155.12"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FSR-ULg","award":["C-02\/66"],"award-info":[{"award-number":["C-02\/66"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1016\/j.rvsc.2014.12.016","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T02:32:44Z","timestamp":1419906764000},"page":"30-36","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"special_numbering":"C","title":["Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep"],"prefix":"10.1016","volume":"99","author":[{"given":"B.","family":"El Amiri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.M.","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Alvarez Oxiley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Hadarbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.F.","family":"Beckers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2014.12.016_bib0010","unstructured":"Ali, M., 1999. Bovine pregnancy-associated glycoprotein in biological fluids (Ph.D. thesis). University of Aberdeen, UK."},{"key":"10.1016\/j.rvsc.2014.12.016_bib0015","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.theriogenology.2009.06.020","article-title":"Validation of a new pregnancy-associated glycoprotein radioimmunoassay method for the detection of early pregnancy in ewes","volume":"72","author":"Barbato","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0020","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0921-4488(01)00242-5","article-title":"Relationship between maternal concentrations of caprine pregnancy-associated glycoprotein in alpine goats and the number of fetuses using a homologous radioimmunoassay","volume":"42","author":"Batalha","year":"2001","journal-title":"Small Rumimant Research"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0025","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1095\/biolreprod26.5.925","article-title":"Detection and partial characterization of two bovine pregnancy specific proteins","volume":"26","author":"Butler","year":"1982","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0030","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/mrd.10246","article-title":"Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta","volume":"64","author":"El Amiri","year":"2003","journal-title":"Molecular Reproduction Development"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0035","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1051\/rnd:2004025","article-title":"Isolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between d 60 and d 100 of gestation","volume":"44","author":"El Amiri","year":"2004","journal-title":"Reproduction, Nutrition, Development"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0040","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1439-0531.2006.00761.x","article-title":"Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep","volume":"42","author":"El Amiri","year":"2007","journal-title":"Reproduction in Domestic Animals"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0045","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1439-0531.2008.01287.x","article-title":"Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection","volume":"45","author":"Friedrich","year":"2010","journal-title":"Reproduction in Domestic Animals"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0050","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1439-0531.2006.00834.x","article-title":"Early detection of pregnancy and embryonic loss in dairy cattle by ELISA tests","volume":"42","author":"G\u00e1bor","year":"2007","journal-title":"Reproduction in Domestic Animals"},{"issue":"Suppl. 9","key":"10.1016\/j.rvsc.2014.12.016_bib0055","first-page":"55","article-title":"Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows","volume":"59","author":"Gajewski","year":"2008","journal-title":"Journal of Physiology and Pharmacology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0060","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.anireprosci.2006.12.002","article-title":"Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants","volume":"103","author":"Garbayo","year":"2008","journal-title":"Animal Reproduction Science"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0065","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/0093-691X(88)90180-X","article-title":"Real-time ultrasonography for determining pregnancy status and viable foetal numbers in ewes","volume":"30","author":"Gearhart","year":"1988","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0070","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/S0093-691X(01)00598-2","article-title":"Pregnancy-associated glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goats","volume":"56","author":"Gonzalez","year":"2001","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0075","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1095\/biolreprod62.6.1624","article-title":"Pregnancy-associated glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy","volume":"62","author":"Green","year":"2000","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0080","first-page":"114","article-title":"The preparation of 131-I labeled human growth hormone of high specific radioactivity","volume":"89","author":"Greenwood","year":"1963","journal-title":"Biochemistry"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0085","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/j.theriogenology.2003.07.018","article-title":"Accuracy of transrectal ultrasonography for determination of pregnancy in sheep: effect of fasting and handling of the animals","volume":"61","author":"Karen","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0090","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.theriogenology.2005.11.017","article-title":"Comparison of accuracy of transabdominal ultrasonography, progesterone and pregnancy-associated glycoproteins tests for discrimination between single and multiple pregnancy in sheep","volume":"66","author":"Karen","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0095","first-page":"269","article-title":"Effect of genotype, age of dams, litter size, birth weight and rams on morphological parameters of the placenta in sheep","volume":"107","author":"Kaulfu\u00df","year":"2000","journal-title":"Deutsche tierarztliche Wochenschrift"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0100","doi-asserted-by":"crossref","first-page":"6316","DOI":"10.3168\/jds.2014-8233","article-title":"A pregnancy detection assay using milk samples: evaluation and considerations","volume":"97","author":"Lawson","year":"2014","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0105","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.3168\/jds.2012-6414","article-title":"Short communication: field evaluation of a pregnancy confirmation test using milk samples in dairy cows","volume":"96","author":"Leblanc","year":"2013","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0110","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.domaniend.2005.12.007","article-title":"Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses","volume":"32","author":"L\u00f3pez-Gatius","year":"2007","journal-title":"Domestic Animal Endocrinology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0115","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1051\/rnd:19930309","article-title":"Peripheral concentrations of a 60-kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle","volume":"33","author":"Mialon","year":"1993","journal-title":"Reproduction, Nutrition, Development"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0120","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1046\/j.1439-0531.2002.t01-1-00366.x","article-title":"Aspartic proteinase members secreted by the ruminant placenta: specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins","volume":"37","author":"Per\u00e9nyi","year":"2002","journal-title":"Reproduction in Domestic Animals"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0125","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1046\/j.1439-0531.2002.00341.x","article-title":"Comparison of the ability of three radioimmunoassays to detect pregnancy-associated glycoproteins in bovine plasma","volume":"37","author":"Per\u00e9nyi","year":"2002","journal-title":"Reproduction in Domestic Animals"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0130","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/0093-691X(94)90691-B","article-title":"Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in Churra and Merino sheep","volume":"42","author":"Ranilla","year":"1994","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0135","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0921-4488(96)00922-4","article-title":"Plasma pregnancy-associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs","volume":"24","author":"Ranilla","year":"1997","journal-title":"Small Ruminant Research"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0140","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1093\/clinchem\/20.10.1255","article-title":"Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays","volume":"20","author":"Rodbard","year":"1974","journal-title":"Clinical Chemistry"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0145","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1095\/biolreprod35.4.936","article-title":"Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation","volume":"35","author":"Sasser","year":"1986","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0205","series-title":"SAS User's Guide","author":"SAS\/STAT Institute","year":"2001"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0150","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1093\/clinchem\/19.2.146","article-title":"Radioimmunossay","volume":"19","author":"Skelley","year":"1973","journal-title":"Clinical Chemistry"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0155","series-title":"Actualit\u00e9 Scientifique Reproduction et Production Laiti\u00e8re","first-page":"129","article-title":"Cin\u00e9tique de la bPAG dans le plasma et dans le lait au cours des trois semaines suivant le part chez la vache laiti\u00e8re","author":"Tainturier","year":"1996"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0160","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1016\/j.theriogenology.2004.08.009","article-title":"Use of a homologous radioimmunoassay (RIA) to evaluate the effect of maternal and foetal parameters on pregnancy-associated glycoprotein (PAG) concentrations in sheep","volume":"63","author":"Vandaele","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0165","doi-asserted-by":"crossref","first-page":"960","DOI":"10.2527\/1995.734960x","article-title":"Detection of fetal twins in sheep using a radioimmunoassay for pregnancy-specific protein B","volume":"73","author":"Willard","year":"1995","journal-title":"Journal of Animal Science"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0170","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/aja.1001700208","article-title":"Role of binucleate cells in fetomaternal cell fusion at implantation in the sheep","volume":"170","author":"Wooding","year":"1984","journal-title":"American Journal of Anatomy"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0175","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.placenta.2004.10.014","article-title":"Light and electron microscope immunocytochemical studies of the distribution of pregnancy-associated glycoproteins (PAGs)","volume":"26","author":"Wooding","year":"2005","journal-title":"Placenta"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0180","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1095\/biolreprod57.6.1384","article-title":"Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue","volume":"57","author":"Xie","year":"1997","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0185","doi-asserted-by":"crossref","first-page":"12809","DOI":"10.1073\/pnas.94.24.12809","article-title":"The diversity and evolutionary relationships of the pregnancy-associated glycoproteins, an aspartic proteinase subfamily consisting of many trophoblast-expressed genes","volume":"94","author":"Xie","year":"1997","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0190","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod45.1.1","article-title":"Purification and characterization of a bovine pregnancy-associated glycoproteins","volume":"45","author":"Zoli","year":"1991","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0195","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1095\/biolreprod46.4.623","article-title":"Light and electron microscopic immunolocalization of bovine pregnancy-associated glycoprotein in the bovine placentome","volume":"46","author":"Zoli","year":"1992","journal-title":"Biology of Reproduction"},{"key":"10.1016\/j.rvsc.2014.12.016_bib0200","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1095\/biolreprod46.1.83","article-title":"Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis","volume":"46","author":"Zoli","year":"1992","journal-title":"Biology of Reproduction"}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528814003579?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528814003579?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T04:00:47Z","timestamp":1758513647000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0034528814003579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":40,"alternative-id":["S0034528814003579"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2014.12.016","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2014.12.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}