{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:30:05Z","timestamp":1784086205326,"version":"3.55.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004881","name":"IMSS","doi-asserted-by":"crossref","award":["2010021"],"award-info":[{"award-number":["2010021"]}],"id":[{"id":"10.13039\/501100004881","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CONACyT","award":["231497"],"award-info":[{"award-number":["231497"]}]},{"name":"CONACyT","award":["168718"],"award-info":[{"award-number":["168718"]}]},{"name":"CONACyT"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.rvsc.2016.02.006","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T13:07:36Z","timestamp":1455023256000},"page":"171-179","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Proteomic and ultrastructural analysis of the effect of a new nitazoxanide-N-methyl-1H-benzimidazole hybrid against Giardia intestinalis"],"prefix":"10.1016","volume":"105","author":[{"given":"F\u00e9lix","family":"Matadamas-Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Hern\u00e1ndez-Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"M\u00e9ndez-Cuesta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanderley","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1807-5938","authenticated-orcid":false,"given":"Ana Paula","family":"Gadelha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjam\u00edn","family":"Nogueda-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili\u00e1n","family":"Y\u00e9pez-Mulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2016.02.006_bb0005","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1159\/000321682","article-title":"Giardia lamblia infection and its implications for vitamin A liver stores in school children","volume":"57","author":"Astiazaran-Garcia","year":"2010","journal-title":"Ann. Nutr. Metab."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0010","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1017\/S003118201200011X","article-title":"Programmed cell death in Giardia","volume":"139","author":"Bagchi","year":"2012","journal-title":"Parasitology"},{"key":"10.1016\/j.rvsc.2016.02.006_bb0015","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1099\/00222615-37-3-221","article-title":"In-vitro susceptibility of Giardia lamblia to albendazole, mebendazole and other chemotherapeutic agents","volume":"37","author":"Cedillo-Rivera","year":"1992","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0020","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1550-7408.2002.tb00523.x","article-title":"In vitro effect of nitazoxanide against Entamoeba histolytica, Giardia intestinalis and Trichomonas vaginalis trophozoites","volume":"49","author":"Cedillo-Rivera","year":"2002","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0025","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/j.1550-7408.1992.tb04841.x","article-title":"Giardia lambla: ultrastructural study of the in vitro effect of benzimidazoles","volume":"39","author":"Chavez","year":"1992","journal-title":"J. Protozool."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0030","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1006\/excr.2002.5496","article-title":"A form of cell death with some features resembling apoptosis in the amitochondrial unicellular organism Trichomonas vaginalis","volume":"276","author":"Chose","year":"2002","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0035","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.parint.2009.08.006","article-title":"Cell death induction in Giardia lamblia: effect of beta-lapachone and starvation","volume":"58","author":"Corr\u00eaa","year":"2009","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0040","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.actatropica.2011.12.009","article-title":"In vitro and in vivo trypanocidal activity of some benzimidazole derivatives against two strains of Trypanosoma cruzi","volume":"122","author":"D\u00edaz-Chiguer","year":"2012","journal-title":"Acta Trop."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0045","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0020-7519(02)00228-X","article-title":"The cytoskeleton of Giardia lamblia","volume":"33","author":"Elmendorf","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0050","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.ijpara.2005.03.009","article-title":"Examination of a novel head-stalk protein family in Giardia lamblia characterised by the pairing of ankyrin repeats and coiled-coil domains","volume":"35","author":"Elmendorf","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0055","first-page":"103","article-title":"Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia","volume":"3","author":"Ghosh","year":"2009","journal-title":"Drug Des. Devel. Ther."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0060","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1645\/GE-1851R.1","article-title":"Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites","volume":"95","author":"Jenkins","year":"2009","journal-title":"J. Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0065","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1128\/EC.00092-12","article-title":"Plasmid vectors for proteomic analyses in Giardia: purification of virulence factors and analysis of the proteasome","volume":"11","author":"Jerlstr\u00f6m-Hultqvist","year":"2012","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.rvsc.2016.02.006_bb0070","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00436-008-1223-x","article-title":"Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia","volume":"104","author":"Kim","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0075","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1756-0500-5-41","article-title":"Proteomic analysis of the ventral disc of Giardia lamblia","volume":"5","author":"Louren\u00e7o","year":"2012","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.rvsc.2016.02.006_bb0080","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.bbrc.2007.08.174","article-title":"Azasterols impair Giardia lamblia proliferation and induces encystation","volume":"363","author":"Maia","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0085","doi-asserted-by":"crossref","first-page":"R66","DOI":"10.1186\/gb-2011-12-7-r66","article-title":"The minimal kinome of Giardia lamblia illuminates early kinase evolution and unique parasite biology","volume":"12","author":"Manning","year":"2011","journal-title":"Genome Biol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0090","doi-asserted-by":"crossref","first-page":"800","DOI":"10.3389\/fmicb.2015.00800","article-title":"Albendazole induces oxidative stress and DNA damage in the parasitic protozoan Giardia duodenalis","volume":"6","author":"Mart\u00ednez-Espinosa","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0095","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/jac\/dkn142","article-title":"Identification of differentially expressed genes in a Giardia lamblia WB C6 clone resistant to nitazoxanide and metronidazole","volume":"62","author":"M\u00fcller","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0100","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1128\/AAC.50.1.162-170.2006","article-title":"In vitro effects of thiazolides on Giardia lamblia WB clone C6 cultured axenically and in coculture with Caco2 cells","volume":"50","author":"M\u00fcller","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0105","doi-asserted-by":"crossref","first-page":"4615","DOI":"10.1016\/S0968-0896(03)00497-8","article-title":"Synthesis and antiparasitic activity of albendazole and mebendazole analogues","volume":"11","author":"Navarrete-V\u00e1zquez","year":"2003","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0110","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.actatropica.2006.03.005","article-title":"Cytotoxic effect of curcumin on Giardia lamblia trophozoites","volume":"98","author":"P\u00e9rez-Arriaga","year":"2006","journal-title":"Acta Trop."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0115","doi-asserted-by":"crossref","first-page":"3251","DOI":"10.1007\/s00436-013-3521-1","article-title":"Albendazole and its derivative JVG9 induce encystation on Giardia intestinalis trophozoites","volume":"112","author":"P\u00e9rez-Rangel","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0120","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.biolcel.2004.05.006","article-title":"The median body of Giardia lamblia: an ultrastructural study","volume":"96","author":"Piva","year":"2004","journal-title":"Biol. Cell."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0125","doi-asserted-by":"crossref","first-page":"7072","DOI":"10.1128\/AAC.02900-14","article-title":"Giardial triosephosphate isomerase as possible target of the cytotoxic effect of omeprazole in Giardia lamblia","volume":"58","author":"Reyes-Vivas","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0130","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.pt.2009.11.010","article-title":"Giardiasis\u2014why do the symptoms sometimes never stop?","volume":"26","author":"Robertson","year":"2010","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0135","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/S0035-9203(97)90531-9","article-title":"Nitazoxanide for the treatment of intestinal protozoan and helminthic infections in Mexico","volume":"91","author":"Romero-Cabello","year":"1997","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0140","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.ijpara.2013.06.001","article-title":"Zoonotic potential of Giardia","volume":"43","author":"Ryan","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0145","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.pt.2006.02.015","article-title":"Giardia and Cryptosporidium join the \u2018Neglected Diseases Initiative\u2019","volume":"22","author":"Savioli","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0150","doi-asserted-by":"crossref","first-page":"6838","DOI":"10.1016\/j.bmcl.2013.10.011","article-title":"Synthesis and antiprotozoal activity of nitazoxanide-N-methylbenzimidazole hybrids","volume":"23","author":"Soria-Arteche","year":"2013","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0155","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1097\/QCO.0b013e32834ad401","article-title":"New approaches to the treatment of giardiasis","volume":"24","author":"Tejman-Yarden","year":"2011","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0160","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1016\/S0960-894X(02)00346-3","article-title":"Synthesis and antiparasitic activity of 1H-benzimidazole derivatives","volume":"12","author":"Valdez","year":"2002","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0165","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1016\/j.bmc.2008.12.059","article-title":"Synthesis and antiprotozoal activity of novel 1-methylbenzimidazole derivatives","volume":"17","author":"Valdez-Padilla","year":"2009","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0170","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.1099\/mic.0.2007\/008599-0","article-title":"Programmed cell death in Entamoeba histolytica induced by the aminoglycoside G418","volume":"153","author":"Villalba","year":"2007","journal-title":"Microbiology"},{"key":"10.1016\/j.rvsc.2016.02.006_bb0175","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.ijpara.2004.12.009","article-title":"Annexin-like alpha giardins: a new cytoskeletal gene family in Giardia lamblia","volume":"35","author":"Weiland","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2016.02.006_bb0180","series-title":"The World Health Report 1996","author":"WHO","year":"1996"},{"key":"10.1016\/j.rvsc.2016.02.006_bb0185","first-page":"15","article-title":"Giardiasis Surveillance United States, 2006\u20132008","volume":"59","author":"Yoder","year":"2010"}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528816300285?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528816300285?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T04:05:31Z","timestamp":1758513931000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0034528816300285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":37,"alternative-id":["S0034528816300285"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2016.02.006","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Proteomic and ultrastructural analysis of the effect of a new nitazoxanide-N-methyl-1H-benzimidazole hybrid against Giardia intestinalis","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2016.02.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}