{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T03:05:29Z","timestamp":1783393529456,"version":"3.54.6"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0501200"],"award-info":[{"award-number":["2017YFD0501200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2015CB150300"],"award-info":[{"award-number":["2015CB150300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872464"],"award-info":[{"award-number":["31872464"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.rvsc.2019.11.013","type":"journal-article","created":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T02:20:35Z","timestamp":1574907635000},"page":"197-204","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Immunodiagnostic potential of recombinant tropomyosin during prepatent Haemonchus contortus infection in goat"],"prefix":"10.1016","volume":"128","author":[{"given":"Muhammad Ali-ul-Husnain","family":"Naqvi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahseen","family":"Jamil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana Zahra","family":"Naqvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ali","family":"Memon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalibixiati","family":"Aimulajiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Tahir","family":"Aleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Ehsan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokai","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruofeng","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2019.11.013_bb0005","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/S1078-1439(98)00009-X","article-title":"Semi-quantitative analysis of telomerase activity of exfoliated cells in urine of patients with urothelial cancers","volume":"3","author":"Akao","year":"1997","journal-title":"Urol. Oncol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0010","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.aca.2004.04.024","article-title":"Electrochemical immunosensor for determination of aflatoxin B 1 in barley","volume":"520","author":"Ammida","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0015","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.actatropica.2013.03.020","article-title":"Diagnosis of Fasciola gigantica infection using a monoclonal antibody-based sandwich ELISA for detection of circulating cathepsin B3 protease","volume":"127","author":"Anuracpreeda","year":"2013","journal-title":"Acta Trop."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0020","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1159\/000048166","article-title":"Identification of continuous, allergenic regions of the major shrimp allergen pen a 1 (tropomyosin)","volume":"127","author":"Ayuso","year":"2002","journal-title":"Int. Arch. Allergy Immunol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.parint.2019.101938","article-title":"Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm","volume":"72","author":"Deo","year":"2019","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0030","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.meegid.2018.12.021","article-title":"Genetic diversity patterns of Haemonchus contortus isolated from sheep and goats in Bangladesh","volume":"68","author":"Dey","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0035","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3844\/ajbbsp.2012.164.170","article-title":"Antigenic and immunogenic components of Haemonchus longistipes identified by western Immunobloting","volume":"8","author":"El-Hassan","year":"2012","journal-title":"Am. J. Biochem. Biotechnol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0040","series-title":"Laboratory Animal Medicine","article-title":"Chapter 24 - Preanesthesia, anesthesia, analgesia, and euthanasia","author":"Flecknell","year":"2015"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0159796","article-title":"Proteomic analysis of the excretory and secretory proteins of Haemonchus contortus (HcESP) binding to goat PBMCs in vivo revealed stage-specific binding profiles","volume":"11","author":"Gadahi","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0050","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.biotechadv.2008.03.003","article-title":"Toward practical, DNA-based diagnostic methods for parasitic nematodes of livestock - bionomic and biotechnological implications","volume":"26","author":"Gasser","year":"2008","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0055","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0020-7519(95)00127-1","article-title":"Identification and partial purification of a 26 kilodalton antigen of adult Haemonchus contortus","volume":"26","author":"Gomez-Mu\u00f1oz","year":"1995","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0060","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1177\/104063870001200409","article-title":"Serodiagnosis of haemonchosis with a somatic antigen (Hc26) in several breeds of sheep","volume":"12","author":"G\u00f3mez-Mu\u00f1oz","year":"2000","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0065","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1007\/s12639-014-0527-2","article-title":"Sero-prevalence of Haemonchus contortus infection in sheep by indirect-ELISA using somatic antigen","volume":"40","author":"Gowda","year":"2016","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0070","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1017\/S0022149X10000763","article-title":"Cloning, expression and characterization of NAD + \u2212dependent glyceraldehyde-3-phosphate dehydrogenase of adult Haemonchus contortus","volume":"85","author":"Han","year":"2011","journal-title":"J. Helminthol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0075","first-page":"238","article-title":"Immunodiagnostic potency of different Haemonchus contortus antigens for diagnosis of experimentally and naturally haemonchosis in Egyptian sheep","volume":"6","author":"Kandil","year":"2015","journal-title":"Acta Parasitol. Glob."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0080","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1007\/s12639-017-0920-8","article-title":"Efficacy of glutathione-S-transferase purified antigen of the gastro-intestinal nematode Haemonchus contortus in diagnosis of sheep haemonchosis","volume":"41","author":"Kandil","year":"2017","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0085","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1007\/s12639-016-0865-3","article-title":"Evaluation of different Haemonchus contortus antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes","volume":"41","author":"Kandil","year":"2017","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0090","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1017\/S0031182096008633","article-title":"Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection","volume":"114","author":"Kooyman","year":"1997","journal-title":"Parasitology"},{"key":"10.1016\/j.rvsc.2019.11.013_bb1000","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0095","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.exppara.2006.09.001","article-title":"Evaluation of a recombinant excretory secretory Haemonchus contortus protein for use in a diagnostic enzyme-linked immunosorbent assay","volume":"115","author":"Li","year":"2007","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0100","article-title":"Comparison of four diagnostic methods for detection and relative quantification of Haemonchus contortus eggs in feces samples","volume":"4","author":"Ljungstr\u00f6m","year":"2017","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0105","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.vetpar.2014.08.020","article-title":"Development and evaluation of serodiagnostic assays with recombinant BgSA1 of Babesia gibsoni","volume":"205","author":"Mandal","year":"2014","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0110","first-page":"26","article-title":"Cellular immune response and abomasum worm burden in goats vaccinated with HC58cDNA vaccine against H. contortus Infection","volume":"13","author":"Muleke","year":"2013","journal-title":"Adv. Life Sci. Technol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0115","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1515\/pjvs-2016-0072","article-title":"Comparison of fecal egg counts and ELISA for the diagnosis of Dicrocoelium dendriticum infection","volume":"19","author":"Naeemipour","year":"2016","journal-title":"Pol. J. Vet. Sci."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0120","doi-asserted-by":"crossref","first-page":"548","DOI":"10.3390\/ani9080548","article-title":"Combined use of indirect ELISA and Western blotting with recombinant hepatocellular carcinoma-associated antigen 59 is a potential immunodiagnostic tool for the detection of prepatent Haemonchus contortus infection in goat","volume":"9","author":"Naqvi","year":"2019","journal-title":"Animals"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0125","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1023\/A:1010303732441","article-title":"Vertebrate tropomyosin: distribution, properties and function","volume":"22","author":"Perry","year":"2001","journal-title":"J. Muscle Res. Cell Motil."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0130","first-page":"94","article-title":"Detection of anti-Haemonchus contortus antibodies in sheep by dot- ELISA with immunoaffinity purified fraction of ES antigen during prepatency","volume":"46","author":"Prasad","year":"2008","journal-title":"Indian J. Exp. Biol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0135","first-page":"90","article-title":"Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests. Open","volume":"5","author":"Rodr\u00edguez","year":"2015","journal-title":"Vet. J."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0140","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/j.biotechadv.2013.01.008","article-title":"Advances in the diagnosis of key gastrointestinal nematode infections of livestock, with an emphasis on small ruminants","volume":"31","author":"Roeber","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0145","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/0304-4017(94)00693-7","article-title":"Comparison of two enzyme immunoassays for the detection of Haemonchus contortus infections in sheep","volume":"57","author":"Schallig","year":"1994","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0150","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3346\/jkms.2018.33.e238","article-title":"Comparison of ELISA and urine microscopy for diagnosis of Schistosoma haematobium infection","volume":"33","author":"Song","year":"2018","journal-title":"J. Korean Med. Sci."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0155","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.mimet.2017.01.008","article-title":"Evaluation of an in-house indirect ELISA for detection of antibody against haemorrhagic septicemia in Asian elephants","volume":"134","author":"Tankaew","year":"2017","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0160","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.mimet.2017.12.005","article-title":"Comparison of two indirect ELISA coating antigens for the detection of dairy cow antibodies against Pasteurella multocida","volume":"145","author":"Tankaew","year":"2017","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0165","first-page":"527","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"19","author":"Towbin","year":"1997","journal-title":"BioEssays"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-019-3375-1","article-title":"Hepatocellular carcinoma-associated antigen 59 of Haemonchus contortus modulates the functions of PBMCs and the differentiation and maturation of monocyte-derived dendritic cells of goats in vitro","volume":"12","author":"Wang","year":"2019","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0175","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.mex.2017.03.002","article-title":"An ELISA protocol to improve the accuracy and reliability of serological antibody assays","volume":"4","author":"Waritani","year":"2017","journal-title":"MethodsX"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0180","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.jim.2012.06.009","article-title":"Enzyme-linked Immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA) - not all BSAs are alike","volume":"384","author":"Xiao","year":"2012","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.rvsc.2019.11.013_bb0185","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000949","article-title":"Evaluation of immunoassays for the diagnosis of Schistosoma japonicum infection using archived sera","volume":"5","author":"Xu","year":"2011","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0190","doi-asserted-by":"crossref","first-page":"16941","DOI":"10.1074\/jbc.M212453200","article-title":"Comprehensive analysis of the secreted proteins of the parasite Haemonchus contortus reveals extensive sequence variation and differential immune recognition","volume":"278","author":"Yatsuda","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.rvsc.2019.11.013_bb0195","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jviromet.2018.12.005","article-title":"Recombinant DENV 2 NS5: an effective antigen for diagnosis of DENV infection","volume":"265","author":"Zhang","year":"2018","journal-title":"J. Virol. Methods"}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528819309336?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528819309336?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:40:48Z","timestamp":1777441248000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0034528819309336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":40,"alternative-id":["S0034528819309336"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2019.11.013","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immunodiagnostic potential of recombinant tropomyosin during prepatent Haemonchus contortus infection in goat","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2019.11.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}