{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T16:41:30Z","timestamp":1772901690385,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307733\/2021\\u20131"],"award-info":[{"award-number":["307733\/2021\\u20131"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.rvsc.2025.105898","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T04:15:16Z","timestamp":1757909716000},"page":"105898","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Parasitological, serological and molecular evaluation of Trypanosoma vivax in cattle experimentally infected and early treated with isometamidium chloride in a region lacking the biological vector"],"prefix":"10.1016","volume":"196","author":[{"given":"Haryie Vict\u00f3ria","family":"Iuasse","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Ferreira","family":"Salvador","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor Maciel Lopes","family":"de Morais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luccas Lourenzzo Lima Lins","family":"Leal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Fellipe Monteiro","family":"Couto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur Siqueira Nunes","family":"Trindade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Lopes","family":"Tamiozo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00eddia Mendes","family":"de Aquino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Souza Azeredo","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena Lopes","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano Ant\u00f4nio","family":"Cadioli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Welber Daniel Zanetti","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2025.105898_bb0005","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1590\/S0074-02761999000200025","article-title":"Clinical, parasitological and immunological aspects of experimental infection with Trypanosoma evansi in dogs","volume":"94","author":"Aquino","year":"1999","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.rvsc.2025.105898_bb0010","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1590\/s1984-29612017019","article-title":"First outbreak and subsequent cases of Trypanosoma vivax in the state of Goi\u00e1s","volume":"26","author":"Bastos","year":"2017","journal-title":"Brazil Rev. Bras Parasitol. Vet."},{"key":"10.1016\/j.rvsc.2025.105898_bb0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.prevetmed.2020.105040","article-title":"Comparison of therapeutic efficacy of different drugs against Trypanosoma vivax on experimentally infected cattle","volume":"181","author":"Bastos","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.rvsc.2025.105898_bb0020","doi-asserted-by":"crossref","DOI":"10.1017\/S0031182020001006","article-title":"Epidemiological and molecular identification of Trypanosoma vivax diagnosed in cattle during outbreaks in Central Brazil","volume":"147","author":"Bastos","year":"2020","journal-title":"Parasitology"},{"issue":"8","key":"10.1016\/j.rvsc.2025.105898_bb0025","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1007\/s00436-021-07226-4","article-title":"Delayed reduction of Anaplasma marginale parasitemia and packed cell volume normalization despite prolonged enrofloxacin treatment of cattle co-infected with Trypanosoma vivax","volume":"120","author":"Bastos","year":"2021","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.rvsc.2025.105898_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2006.08.017","article-title":"Trypanosomiasis by Trypanosoma vivax in cattle in the Brazilian semiarid: description of an outbreak and lesions in the nervous system","volume":"143","author":"Batista","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0035","doi-asserted-by":"crossref","DOI":"10.1590\/S0100-736X2008000100010","article-title":"Aspectos cl\u00ednicos, epidemiol\u00f3gicos e patol\u00f3gicos da infec\u00e7\u00e3o natural em bovinos por Trypanosoma vivax na Para\u00edba","volume":"28","author":"Batista","year":"2008","journal-title":"Pesqui. Vet. Bras."},{"key":"10.1016\/j.rvsc.2025.105898_bb0040","article-title":"Clinical evaluation and reproductive indices of dairy cows naturally infected with Trypanosoma vivax","volume":"38","author":"Batista","year":"2017","journal-title":"Semin Agrar"},{"key":"10.1016\/j.rvsc.2025.105898_bb0045","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and evaluation of a seminested PCR for detection and differentiation of babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples","volume":"41","author":"Birkenheuer","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0050","series-title":"Contribui\u00e7\u00e3o ao estudo da terap\u00eautica experimental da Doen\u00e7a de Chagas","author":"Brener","year":"1961"},{"key":"10.1016\/j.rvsc.2025.105898_bb0055","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1590\/S1984-29612012000200009","article-title":"First report of Trypanosoma vivax outbreak in dairy cattle in S\u00e3o Paulo state, Brazil","volume":"21","author":"Cadioli","year":"2012","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.rvsc.2025.105898_bb0060","article-title":"Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatment with isometamidium chloride","volume":"30","author":"Castilho-Neto","year":"2021","journal-title":"Braz. J. Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0065","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.mcp.2008.11.003","article-title":"Cathepsin L-like genes of Trypanosoma vivax from Africa and South America - characterization, relationships and diagnostic implications","volume":"23","author":"Cortez","year":"2009","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.rvsc.2025.105898_bb0070","article-title":"In vitro and in vivo effectiveness of disinfectants against Trypanosoma vivax","volume":"25","author":"Couto","year":"2021","journal-title":"Vet. Parasitol. Reg. Stud. Reports."},{"key":"10.1016\/j.rvsc.2025.105898_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2022.109761","article-title":"Presence of Trypanosoma vivax DNA in cattle semen and reproductive tissues and related changes in sperm parameters","volume":"309","author":"Couto","year":"2022","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2024.110221","article-title":"Reproductive, productive and financial consequences of chronic Trypanosoma vivax infection in a dairy cattle herd in a region without a cyclic vector","volume":"330","author":"Couto","year":"2024","journal-title":"Vet. Parasitol."},{"issue":"109","key":"10.1016\/j.rvsc.2025.105898_bb0085","article-title":"Pharmacokinetics and efficacy of isometamidium chloride against Trypanosoma vivax in cattle","volume":"14","author":"Couto","year":"2025","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.rvsc.2025.105898_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2025.110553","article-title":"Can the tick Rhipicephalus microplus transmit Trypanosoma vivax to cattle by the transovarial and mechanical routes?","volume":"338","author":"Couto","year":"2025","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0095","first-page":"41","article-title":"Review on Trypanosoma vivax","volume":"7","author":"Dagnachew","year":"2015","journal-title":"African J. Basic Appl. Sci."},{"key":"10.1016\/j.rvsc.2025.105898_bb0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.rvsc.2024.105290","article-title":"Trypanosoma vivax in and outside cattle blood: parasitological, molecular, and serological detection, reservoir tissues, histopathological lesions, and vertical transmission evaluation","volume":"174","author":"De Melo-Junior","year":"2024","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.rvsc.2025.105898_bb0100","article-title":"How many cattle can be infected by Trypanosoma vivax by reusing the same needle and syringe, and what is the viability time of this protozoan in injectable veterinary products?","volume":"1\u201313","author":"De Melo-Junior","year":"2021","journal-title":"Parasitology"},{"key":"10.1016\/j.rvsc.2025.105898_bb0110","article-title":"First record of a possible trypanotolerant cattle breed in Latin America: parasitological, serological, and clinical aspects","volume":"54","author":"De Mendon\u00e7a","year":"2024","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"10.1016\/j.rvsc.2025.105898_bb0115","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-022-05190-1","article-title":"A review on the diagnosis of animal trypanosomoses","author":"Desquesnes","year":"2022","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.rvsc.2025.105898_bb0120","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-022-05352-1","article-title":"Diagnosis of animal trypanosomoses: proper use of current tools and future prospects","author":"Desquesnes","year":"2022","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.rvsc.2025.105898_bb0125","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-021-04584-x","article-title":"Global distribution, host range and prevalence of Trypanosoma vivax: a systematic review and meta-analysis","volume":"14","author":"Fetene","year":"2021","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.rvsc.2025.105898_bb0130","doi-asserted-by":"crossref","DOI":"10.1590\/S1984-29612016013","article-title":"Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax","volume":"25","author":"Fidelis Junior","year":"2016","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.rvsc.2025.105898_bb0135","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1471-4922(00)01827-4","article-title":"African bovine trypanosomiasis: the problem of drug resistance","volume":"17","author":"Geerts","year":"2001","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0140","doi-asserted-by":"crossref","DOI":"10.1017\/S0031182016001268","article-title":"The animal trypanosomiases and their chemotherapy: a review","volume":"143","author":"Giordani","year":"2016","journal-title":"Parasitol"},{"key":"10.1016\/j.rvsc.2025.105898_bb0145","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1007\/s00436-023-08102-z","article-title":"Evaluation of mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans in a region without a cyclic vector","volume":"123","author":"Heller","year":"2024","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.rvsc.2025.105898_bb0150","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.828111","article-title":"An update on African trypanocide pharmaceutics and resistance","volume":"9","author":"Kasozi","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.rvsc.2025.105898_bb0155","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0304-4017(97)00003-4","article-title":"An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against babesia bovis in cattle","volume":"71","author":"Machado","year":"1997","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0160","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1590\/S1984-29612008000100002","article-title":"A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test","volume":"17","author":"Oliveira","year":"2008","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.rvsc.2025.105898_bb0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0074-02762008000100001","article-title":"Trypanosoma (Duttonella) vivax: its biology, epidemiology, pathogenesis, and introduction in the New World-a review","volume":"103","author":"Osorio","year":"2008","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.rvsc.2025.105898_bb0170","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.pt.2021.04.007","article-title":"Pharma to farmer: field challenges of optimizing trypanocide use in African animal trypanosomiasis","volume":"37","author":"Richards","year":"2021","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0175","article-title":"Tissue tropism in parasitic diseases","volume":"9","author":"Silva-Pereira","year":"2019","journal-title":"Open Biol."},{"key":"10.1016\/j.rvsc.2025.105898_bb0180","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1017\/S0031182000066737","article-title":"Extravascular foci of Trypanosoma vivax in goats: the central nervous system and aqueous humor of the eye as potential sources of relapse infections after chemotherapy","volume":"97","author":"Whitelaw","year":"1988","journal-title":"Parasitol"},{"key":"10.1016\/j.rvsc.2025.105898_bb0185","first-page":"384","article-title":"The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis","volume":"27","author":"Woo","year":"1970","journal-title":"Acta Trop."}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528825003728?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0034528825003728?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T22:37:47Z","timestamp":1772836667000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0034528825003728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":37,"alternative-id":["S0034528825003728"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2025.105898","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Parasitological, serological and molecular evaluation of Trypanosoma vivax in cattle experimentally infected and early treated with isometamidium chloride in a region lacking the biological vector","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2025.105898","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105898"}}