{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T10:26:04Z","timestamp":1786271164996,"version":"build-2736575974"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Research in Veterinary Science"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.rvsc.2026.106248","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T22:58:36Z","timestamp":1778108316000},"page":"106248","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Diagnostic value of digital PCR (dPCR) in the diagnosis of leishmania infantum from canine chronic dermatitis"],"prefix":"10.1016","volume":"208","author":[{"given":"Leonardo","family":"Brustenga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Brachelente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Morganti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Porcellato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Gavaudan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Canonico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizia","family":"Veronesi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Capomaccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.rvsc.2026.106248_bb0005","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/s13071-023-05756-7","article-title":"Digital PCR: modern solution to parasite diagnostics and population trait genetics","volume":"16","author":"Baltru\u0161is","year":"2023","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.rvsc.2026.106248_bb0010","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.tvjl.2006.11.011","article-title":"Canine leishmaniasis: a diagnostic and clinical challenge","volume":"175","author":"Baneth","year":"2008","journal-title":"Vet. J."},{"issue":"8476","key":"10.1016\/j.rvsc.2026.106248_bb0015","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0140-6736(86)90837-8","article-title":"Statistical methods for assessing agreement between two methods of clinical measurement","volume":"1","author":"Bland","year":"1986","journal-title":"Lancet"},{"issue":"7","key":"10.1016\/j.rvsc.2026.106248_bb0020","doi-asserted-by":"crossref","first-page":"865","DOI":"10.3390\/pathogens10070865","article-title":"Molecular diagnosis of leishmaniasis: quantification of parasite load by a real-time PCR assay with high sensitivity","volume":"10","author":"Castelli","year":"2021","journal-title":"Pathogens"},{"key":"10.1016\/j.rvsc.2026.106248_bb0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2021.109368","article-title":"Correlations between tissue parasite load and common clinical signs in dogs naturally infected by leishmania infantum","volume":"291","author":"Chagas","year":"2021","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0001596","article-title":"Canine skin and conjunctival swab samples for the detection and quantification of leishmania infantum DNA in an endemic urban area in Brazil","volume":"6","author":"de Almeida Ferreira","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.rvsc.2026.106248_bb0035","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1128\/JCM.00558-12","article-title":"Diagnostic value of conjunctival swab sampling associated with nested PCR for different categories of dogs naturally exposed to leishmania infantum infection","volume":"50","author":"Di Muccio","year":"2012","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.rvsc.2026.106248_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0077771","article-title":"Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition","volume":"8","author":"Dietrich","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.rvsc.2026.106248_bb0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006922","article-title":"Validation of SYBR green\u00a0I based closed-tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL)","volume":"12","author":"Dixit","year":"2018","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.rvsc.2026.106248_bb0050","series-title":"Veterinary Epidemiologic Research","author":"Dohoo","year":"2009"},{"key":"10.1016\/j.rvsc.2026.106248_bb0055","author":"Dorai-Raj"},{"key":"10.1016\/j.rvsc.2026.106248_bb0060","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.vetpar.2007.12.022","article-title":"Evaluation of the conjunctival swab for canine visceral leishmaniasis diagnosis by PCR-hybridization in Minas Gerais state, Brazil","volume":"152","author":"Ferreira","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0065","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/j.1750-3639.2007.00073.x","article-title":"Effects of formalin fixation, paraffin embedding, and time of storage on DNA preservation in brain tissue: a BrainNet Europe study","volume":"17","author":"Ferrer","year":"2007","journal-title":"Brain Pathol."},{"issue":"6","key":"10.1016\/j.rvsc.2026.106248_bb0070","doi-asserted-by":"crossref","first-page":"634","DOI":"10.3390\/pathogens11060634","article-title":"Detection of leishmania spp. in chronic dermatitis: retrospective study in exposed horse populations","volume":"11","author":"Gazzonis","year":"2022","journal-title":"Pathogens"},{"key":"10.1016\/j.rvsc.2026.106248_bb0075","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jcpa.2006.06.005","article-title":"Relationship between canine visceral leishmaniasis and the leishmania (leishmania) chagasi burden in dermal inflammatory foci","volume":"135","author":"Giunchetti","year":"2006","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0080","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.ijpara.2005.07.001","article-title":"The current status of zoonotic leishmaniases and approaches to disease control","volume":"35","author":"Gramiccia","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0085","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1006\/meth.2001.1263","article-title":"Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies","volume":"25","author":"Lehmann","year":"2001","journal-title":"Methods"},{"issue":"1","key":"10.1016\/j.rvsc.2026.106248_bb0090","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2307\/2532051","article-title":"A concordance correlation coefficient to evaluate reproducibility","volume":"45","author":"Lin","year":"1989","journal-title":"Biometrics"},{"key":"10.1016\/j.rvsc.2026.106248_bb0095","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.rvsc.2009.03.008","article-title":"Parasitological diagnosis of canine visceral leishmaniasis: is intact skin a good target?","volume":"87","author":"Madeira","year":"2009","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.rvsc.2026.106248_bb0100","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tvjl.2007.08.009","article-title":"Diagnosis of canine leishmaniasis: conventional and molecular techniques using different tissues","volume":"179","author":"Maia","year":"2009","journal-title":"Vet. J."},{"key":"10.1016\/j.rvsc.2026.106248_bb0105","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.rvsc.2008.12.009","article-title":"Evidence for a relationship between leishmania load and clinical manifestations","volume":"87","author":"Manna","year":"2009","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.rvsc.2026.106248_bb0110","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/1756-3305-4-57","article-title":"Canine leishmaniasis: the key points for qPCR result interpretation","volume":"4","author":"Mart\u00ednez","year":"2011","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.rvsc.2026.106248_bb0115","article-title":"Clinical, molecular and serological diagnosis of canine leishmaniosis: an integrated approach","volume":"7","author":"Maurelli","year":"2020","journal-title":"Vet. Sci."},{"key":"10.1016\/j.rvsc.2026.106248_bb0120","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/BF02295996","article-title":"Note on the use of the chi-square statistic for 2 \u00d7 2 contingency tables","volume":"12","author":"McNemar","year":"1947","journal-title":"Psychometrika"},{"issue":"9","key":"10.1016\/j.rvsc.2026.106248_bb0125","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.pt.2017.05.005","article-title":"Novel areas for prevention and control of canine leishmaniosis","volume":"33","author":"Mir\u00f3","year":"2017","journal-title":"Trends Parasitol."},{"issue":"3","key":"10.1016\/j.rvsc.2026.106248_bb0130","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0304-4017(03)00139-0","article-title":"PCR-based detection of canine leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction","volume":"114","author":"M\u00fcller","year":"2003","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0135","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1007\/s11010-023-04672-9","article-title":"Development of quantitative PCR and digital PCR for the quantification of leishmania infantum in dogs","volume":"478","author":"Pereira","year":"2023","journal-title":"Mol. Cell. Biochem."},{"issue":"12","key":"10.1016\/j.rvsc.2026.106248_bb0140","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.cmi.2019.06.009","article-title":"Digital PCR: a new technology for diagnosis of parasitic infections","volume":"25","author":"Pomari","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"issue":"4","key":"10.1016\/j.rvsc.2026.106248_bb0145","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/mve.12464","article-title":"Molecular detection of Leishmania (Sauroleishmania) tarentolae in human blood and Leishmania (Leishmania) infantum in Sergentomyia minuta: unexpected host-parasite contacts","volume":"34","author":"Pombi","year":"2020","journal-title":"Med. Vet. Entomol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0150","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-022-05218-6","article-title":"Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of Leishmania in dogs living in endemic areas: a preliminary study","volume":"15","author":"Porcellato","year":"2022","journal-title":"Parasit. Vectors"},{"issue":"3\u20134","key":"10.1016\/j.rvsc.2026.106248_bb0155","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.vetpar.2013.07.006","article-title":"Molecular diagnosis of canine visceral leishmaniasis: a comparative study of three methods using skin and spleen from dogs with natural Leishmania infantum infection","volume":"197","author":"Reis","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0160","series-title":"Psych: Procedures for Psychological, Psychometric, and Personality Research","author":"Revelle","year":"2025"},{"issue":"4","key":"10.1016\/j.rvsc.2026.106248_bb0165","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1097\/00019606-200112000-00009","article-title":"Comparison of the DNA extraction methods for PCR amplification from formalin-fixed and paraffin-embedded tissues","volume":"10","author":"Sato","year":"2001","journal-title":"Diagn. Mol. Pathol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0170","series-title":"DescTools: Tools for Descriptive Statistics","author":"Signorelli","year":"2025"},{"key":"10.1016\/j.rvsc.2026.106248_bb0175","unstructured":"Slowikowski, K., 2024. Ggrepel: Automatically Position Non-Overlapping Text Labels [R package]. https:\/\/CRAN.R-project.org\/package=ggrepel."},{"issue":"1\u20132","key":"10.1016\/j.rvsc.2026.106248_bb0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2009.05.022","article-title":"Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis","volume":"165","author":"Solano-Gallego","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.rvsc.2026.106248_bb0185","doi-asserted-by":"crossref","DOI":"10.1186\/1756-3305-4-86","article-title":"LeishVet guidelines for the practical management of canine leishmaniosis","volume":"4","author":"Solano-Gallego","year":"2011","journal-title":"Parasit. Vectors"},{"issue":"2","key":"10.1016\/j.rvsc.2026.106248_bb0190","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s12639-020-01212-w","article-title":"Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods","volume":"44","author":"Thakur","year":"2020","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.rvsc.2026.106248_bb0195","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1196\/annals.1307.018","article-title":"TaqMan-based detection of leishmania infantum DNA using canine samples","volume":"1026","author":"Vitale","year":"2004","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.rvsc.2026.106248_bb0200","doi-asserted-by":"crossref","first-page":"6323","DOI":"10.1007\/s11033-023-08491-5","article-title":"The quality of DNA isolated from autopsy formalin-fixed and paraffin-embedded tissues: study of 1662 samples","volume":"50","author":"Vito\u0161evi\u0107","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.rvsc.2026.106248_bb0205","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"10.1016\/j.rvsc.2026.106248_bb0210","series-title":"dplyr: A Grammar of Data Manipulation [R package]","author":"Wickham","year":"2023"}],"container-title":["Research in Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003452882600202X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S003452882600202X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:36:10Z","timestamp":1785159370000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S003452882600202X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":42,"alternative-id":["S003452882600202X"],"URL":"https:\/\/doi.org\/10.1016\/j.rvsc.2026.106248","relation":{},"ISSN":["0034-5288"],"issn-type":[{"value":"0034-5288","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Diagnostic value of digital PCR (dPCR) in the diagnosis of leishmania infantum from canine chronic dermatitis","name":"articletitle","label":"Article Title"},{"value":"Research in Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.rvsc.2026.106248","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"106248"}}