{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T03:49:42Z","timestamp":1768794582523,"version":"3.49.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1016\/j.saa.2021.119988","type":"journal-article","created":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T12:33:12Z","timestamp":1621600392000},"page":"119988","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["A sensitive bio-probe for tracking lipid droplets with large Stokes shift and its application in cell imaging"],"prefix":"10.1016","volume":"260","author":[{"given":"Yizhe","family":"Yan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoqing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Hao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbo","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Yong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.saa.2021.119988_b0005","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.ceb.2008.05.009","article-title":"Cell biology of lipid droplets","volume":"20","author":"Thiele","year":"2008","journal-title":"Curr. Opin. Cell. Biol."},{"key":"10.1016\/j.saa.2021.119988_b0010","doi-asserted-by":"crossref","unstructured":"R. V, F. Jr, T. C. Walther, Lipid droplets finally get a little R-E-S-P-E-C-T, Cell 139(5) (2009) 855-860.","DOI":"10.1016\/j.cell.2009.11.005"},{"issue":"11","key":"10.1016\/j.saa.2021.119988_b0015","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1016\/j.febslet.2010.03.022","article-title":"Lipid droplets: A dynamic organelle moves into focus","volume":"584","author":"Beller","year":"2010","journal-title":"Febs Lett."},{"key":"10.1016\/j.saa.2021.119988_b0020","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3389\/fcell.2015.00049","article-title":"The lipid droplet-a well-connected organelle","volume":"3","author":"Gao","year":"2015","journal-title":"Front. Cell. Dev. Biol."},{"issue":"1\u20132","key":"10.1016\/j.saa.2021.119988_b0025","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.cell.2014.12.019","article-title":"Glial lipid droplets and ROS induced by mitochondrial defects promote neurodegeneration","volume":"160","author":"Liu","year":"2015","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.saa.2021.119988_b0030","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1007\/BF01033631","article-title":"Imidazole-buffered osmium tetroxide: an excellent stain for visualization of lipids in transmission electron microscopy","volume":"14","author":"Angerm\u00fcller","year":"1982","journal-title":"Histochem. J."},{"issue":"1","key":"10.1016\/j.saa.2021.119988_b0035","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s004180100297","article-title":"Optimistation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids","volume":"116","author":"Koopman","year":"2001","journal-title":"Histochem. Cell. Biol."},{"issue":"13","key":"10.1016\/j.saa.2021.119988_b0040","doi-asserted-by":"crossref","first-page":"6666","DOI":"10.1021\/ac501395g","article-title":"Raman imaging providing insights into chemical composition of lipid droplets of different size and origin: in hepatocytes and endothelium","volume":"86","author":"Majzner","year":"2014","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.saa.2021.119988_b0045","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1021\/cr2004103","article-title":"Luminescent chemodosimeters for bioimaging","volume":"113","author":"Yang","year":"2013","journal-title":"Chem. Rev."},{"issue":"12","key":"10.1016\/j.saa.2021.119988_b0050","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1039\/c2cs00004k","article-title":"Fluorescent chemodosimeters using \u201cmild\u201d chemical events for the detection of small anions and cations in biological and environmental media","volume":"41","author":"Du","year":"2012","journal-title":"Chem. Soc. Rev."},{"issue":"19","key":"10.1016\/j.saa.2021.119988_b0055","doi-asserted-by":"crossref","first-page":"7943","DOI":"10.1039\/c3cs60123d","article-title":"Colourimetric and fluorescent probes for the optical detection of palladium ions","volume":"42","author":"Li","year":"2013","journal-title":"Chem. Soc. Rev."},{"issue":"1","key":"10.1016\/j.saa.2021.119988_b0060","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1021\/cr300508p","article-title":"Design strategies for water-soluble small molecular chromogenic and fluorogenic probes","volume":"114","author":"Li","year":"2014","journal-title":"Chem. Rev."},{"issue":"14","key":"10.1016\/j.saa.2021.119988_b0065","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1039\/C4CS00298A","article-title":"Chemical probes for molecular imaging and detection of hydrogen sulfide and reactive sulfur species in biological systems","volume":"44","author":"Lin","year":"2015","journal-title":"Chem. Soc. Rev."},{"issue":"15","key":"10.1016\/j.saa.2021.119988_b0070","doi-asserted-by":"crossref","first-page":"7944","DOI":"10.1021\/cr500567r","article-title":"Recent progress on the development of chemsensors for gases","volume":"115","author":"Zhou","year":"2015","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2021.119988_b0075","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.trac.2015.11.014","article-title":"Recent progress in the fluorescent probes for the specific imaging of small molecular weight thiols in living cells","volume":"76","author":"Chen","year":"2016","journal-title":"Trac-Trend. Anal. Chem."},{"issue":"29","key":"10.1016\/j.saa.2021.119988_b0080","doi-asserted-by":"crossref","first-page":"7676","DOI":"10.1039\/D0SC01213K","article-title":"Cancer cell discrimination and dynamic viability monitoring through wash-free bioimaging using AIEgens","volume":"11","author":"Zhang","year":"2020","journal-title":"Chem. Sci."},{"issue":"9","key":"10.1016\/j.saa.2021.119988_b0085","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1039\/D0AY02247K","article-title":"Construction of fluorescent rotors with multiple intramolecular rotation sites for visualization of cellular viscous compartments with elevated fidelity","volume":"13","author":"Hao","year":"2021","journal-title":"Anal. Methods"},{"issue":"3","key":"10.1016\/j.saa.2021.119988_b0090","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1083\/jcb.100.3.965","article-title":"Nile red: a selective fluorescent stain for intracellular lipid droplets","volume":"100","author":"Greenspan","year":"1985","journal-title":"The J. Cell Biol."},{"key":"10.1016\/j.saa.2021.119988_b0095","first-page":"598358","article-title":"Use of BODIPY (493\/503) to visualize intramuscular lipid droplets in skeletal muscle","author":"Spangenburg","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"issue":"15","key":"10.1016\/j.saa.2021.119988_b0100","doi-asserted-by":"crossref","first-page":"4622","DOI":"10.1002\/ange.201500229","article-title":"Environment-sensitive fluorescent probe: a benzophosphole oxide with an electron-donating substituent","volume":"127","author":"Yamaguchi","year":"2015","journal-title":"Angew. Chem. Int. Ed."},{"issue":"5","key":"10.1016\/j.saa.2021.119988_b0105","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1039\/C6CC09471F","article-title":"An easily accessible aggregation-induced emission probe for lipid droplet-specific imaging and movement tracking","volume":"53","author":"Gao","year":"2017","journal-title":"Chem. Commun."},{"issue":"19","key":"10.1016\/j.saa.2021.119988_b0110","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1002\/asia.201701010","article-title":"Precise labeling and tracking of lipid droplets in adipocytes using a luminescent ZnSalen complex","volume":"12","author":"Tang","year":"2017","journal-title":"Chem-Asian. J."},{"key":"10.1016\/j.saa.2021.119988_b0115","doi-asserted-by":"crossref","first-page":"3148","DOI":"10.1016\/j.snb.2017.09.139","article-title":"A specific bioprobe for super-resolution fluorescence imaging of lipid droplets","volume":"255","author":"Zheng","year":"2018","journal-title":"Sensor. Actuat. B-Chem."},{"issue":"42","key":"10.1016\/j.saa.2021.119988_b0120","doi-asserted-by":"crossref","first-page":"23727","DOI":"10.1021\/acs.jpcc.7b07571","article-title":"Quantum dot-based designed nanoprobe for imaging lipid droplet","volume":"121","author":"Mandal","year":"2017","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.saa.2021.119988_b0125","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.snb.2018.02.022","article-title":"Lipophilic phosphorescent iridium (III) complexes as one-and two-photon selective bioprobes for lipid droplets imaging in living cells","volume":"262","author":"He","year":"2018","journal-title":"Sensor. Actuat. B-Chem."},{"issue":"13","key":"10.1016\/j.saa.2021.119988_b0130","doi-asserted-by":"crossref","first-page":"10706","DOI":"10.1021\/acsami.8b00278","article-title":"Interface-targeting strategy enables two-photon fluorescent lipid droplet probes for high-fidelity imaging of turbid tissues and detecting fatty liver","volume":"10","author":"Guo","year":"2018","journal-title":"ACS Appl. Mater. Inter."},{"issue":"14","key":"10.1016\/j.saa.2021.119988_b0135","doi-asserted-by":"crossref","first-page":"4778","DOI":"10.1021\/acs.chemmater.8b01943","article-title":"Specific two-photon imaging of live cellular and deep-tissue lipid droplets by lipophilic AIEgens at ultralow concentration","volume":"30","author":"Niu","year":"2018","journal-title":"Chem. Mater."},{"issue":"11","key":"10.1016\/j.saa.2021.119988_b0140","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.1021\/acs.bioconjchem.8b00552","article-title":"Imaging and quantitative detection of lipid droplets by yellow fluorescent probes in liver sections of plasmodium infected mice and third stage human cervical cancer tissues","volume":"29","author":"Sharma","year":"2018","journal-title":"Bioconjugate Chem."},{"issue":"6","key":"10.1016\/j.saa.2021.119988_b0145","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1021\/acs.biochem.8b01071","article-title":"Monitoring lipid droplet dynamics in living cells by using fluorescent probes","volume":"58","author":"Tatenaka","year":"2019","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2021.119988_b0150","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.snb.2018.12.162","article-title":"An AIEgen-based fluorescent probe for highly selective and specific imaging of lipid droplets in L02 and HepG2 cells","volume":"284","author":"Shi","year":"2019","journal-title":"Sensor. Actuat. B-Chem."},{"key":"10.1016\/j.saa.2021.119988_b0155","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.snb.2019.02.122","article-title":"Two-photon fluorescence imaging of lipid drops polarity toward cancer diagnosis in living cells and tissue","volume":"288","author":"Yin","year":"2019","journal-title":"Sensor. Actuat. B-Chem."},{"issue":"23","key":"10.1016\/j.saa.2021.119988_b0160","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1039\/C9TB00421A","article-title":"Identification of fatty liver disease at diverse stages using two-photon absorption of triphenylamine-based BODIPY analogues","volume":"7","author":"Shao","year":"2019","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.saa.2021.119988_b0165","doi-asserted-by":"crossref","first-page":"107718","DOI":"10.1016\/j.dyepig.2019.107718","article-title":"A rationally designed polarity-viscosity sensitive probe for imaging lipid droplets","volume":"171","author":"Song","year":"2019","journal-title":"Dyes Pigments"},{"issue":"3","key":"10.1016\/j.saa.2021.119988_b0170","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1021\/acs.analchem.8b04218","article-title":"Probing polarity and heterogeneity of lipid droplets in live cells using a push-pull fluorophore","volume":"91","author":"Collot","year":"2018","journal-title":"Anal. Chem."},{"issue":"16","key":"10.1016\/j.saa.2021.119988_b0175","doi-asserted-by":"crossref","first-page":"5401","DOI":"10.1021\/jacs.7b12817","article-title":"Ultrabright and fluorogenic probes for multicolor imaging and tracking of lipid droplets in cells and tissues","volume":"140","author":"Collot","year":"2018","journal-title":"J. Am. Chem. Soc."},{"issue":"7","key":"10.1016\/j.saa.2021.119988_b0180","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1021\/acs.analchem.9b05184","article-title":"Visualization of lipid droplets in living cells and fatty livers of mice based on the fluorescence of \u03c0-extended coumarin using fluorescence lifetime imaging microscopy","volume":"92","author":"Yoshihara","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2021.119988_b0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2020.129148","article-title":"Simultaneous visualization of lipid droplets and lysosomes using a single fluorescent probe","volume":"329","author":"Meng","year":"2021","journal-title":"Sensor. Actuat. B-Chem."},{"key":"10.1016\/j.saa.2021.119988_b0190","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1021\/acssensors.0c02015","article-title":"Revealing the viscosity changes in lipid droplets during ferroptosis by the real-time and in situ near-infrared imaging","volume":"6","author":"Dong","year":"2021","journal-title":"Acs Sens."},{"issue":"9","key":"10.1016\/j.saa.2021.119988_b0195","doi-asserted-by":"crossref","first-page":"4347","DOI":"10.1039\/D0NJ05900E","article-title":"A fluorogenic probe for dynamic tracking of lipid droplets\u2019 polarity during the evolution of cancer","volume":"45","author":"Liu","year":"2021","journal-title":"New J. Chem."},{"key":"10.1016\/j.saa.2021.119988_b0200","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1021\/jacs.0c12323","article-title":"Simultaneous two-color visualization of lipid droplets and endoplasmic reticulum and their interplay by single fluorescent probes in lambda mode","volume":"143","author":"Guo","year":"2021","journal-title":"J. Am. Chem. Soc."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142521005655?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142521005655?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T01:45:25Z","timestamp":1761356725000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142521005655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":40,"alternative-id":["S1386142521005655"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2021.119988","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A sensitive bio-probe for tracking lipid droplets with large Stokes shift and its application in cell imaging","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2021.119988","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"119988"}}