{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T21:20:53Z","timestamp":1793654453178,"version":"4.5.1"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.saa.2022.121394","type":"journal-article","created":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T19:30:32Z","timestamp":1653161432000},"page":"121394","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Optical absorbance-based rapid test for the detection of sickle cell trait and sickle cell disease at the point-of-care"],"prefix":"10.1016","volume":"279","author":[{"given":"Rajesh","family":"Srinivasan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.R.","family":"Eugene Christo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhika","family":"Nambannor Kunnath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prateek","family":"Katare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravind","family":"Venukumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisanth K.M.","family":"Nambison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai Siva","family":"Gorthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9079","key":"10.1016\/j.saa.2022.121394_b0005","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0140-6736(97)07330-3","article-title":"Sickle-cell disease","volume":"350","author":"Serjeant","year":"1997","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.saa.2022.121394_b0010","first-page":"1193","article-title":"Sickle cell disease","volume":"49","author":"Fixler","year":"2002","journal-title":"Pediatri. Clin."},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-36077-w","article-title":"The spatial epidemiology of sickle-cell anaemia in india","volume":"8","author":"Hockham","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.saa.2022.121394_b0020","series-title":"Sickle cell disease: basic principles and clinical practice","author":"Hebbel","year":"1994"},{"key":"10.1016\/j.saa.2022.121394_b0025","article-title":"Sickle cell anemia","author":"Mangla","year":"2021","journal-title":"StatPearls"},{"issue":"5880","key":"10.1016\/j.saa.2022.121394_b0030","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/299231a0","article-title":"The effects of \u03b1chain mutations cis and trans to the \u03b26 mutation on the polymerization of sickle cell haemoglobin","volume":"299","author":"Benesch","year":"1982","journal-title":"Nature"},{"issue":"5750","key":"10.1016\/j.saa.2022.121394_b0035","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1038\/283832a0","article-title":"\u03b2-chain contact sites in the haemoglobin s polymer","volume":"283","author":"Nagel","year":"1980","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0040","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0950-3536(98)80074-7","article-title":"6 pathophysiology of sickle cell disease","volume":"11","author":"Steinberg","year":"1998","journal-title":"Bailli\u00e8re\u2019s Clin. Haematol."},{"issue":"7","key":"10.1016\/j.saa.2022.121394_b0045","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1177\/1535370216640385","article-title":"Minireview: clinical severity in sickle cell disease: the challenges of definition and prognostication","volume":"241","author":"Quinn","year":"2016","journal-title":"Exp. Biol. Med."},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0050","first-page":"1","article-title":"Sickle cell disease","volume":"4","author":"Kato","year":"2018","journal-title":"Nat. Rev. Dis. Prim."},{"key":"10.1016\/j.saa.2022.121394_b0055","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.cca.2014.10.006","article-title":"Advances in detection of hemoglobinopathies","volume":"439","author":"Greene","year":"2015","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.saa.2022.121394_b0060","series-title":"The peripheral blood film","author":"Arthur Harper","year":"1974"},{"issue":"4","key":"10.1016\/j.saa.2022.121394_b0065","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1093\/labmed\/6.4.38","article-title":"A solubility test for sickle cell-hemoglobin","volume":"6","author":"Diggs","year":"1975","journal-title":"Lab. Med."},{"key":"10.1016\/j.saa.2022.121394_b0070","doi-asserted-by":"crossref","unstructured":"V.N. Tubman, J.J. Field, Sickle solubility test to screen for sickle cell trait: what\u2019s the harm?, Hematology 2014, the American Society of Hematology Education Program Book 2015 (1) (2015) 433\u2013435.","DOI":"10.1182\/asheducation-2015.1.433"},{"key":"10.1016\/j.saa.2022.121394_b0075","doi-asserted-by":"crossref","unstructured":"A. Okwi, W. Byarugaba, A. Parkes, M. Ocaido, The reliability of sickling and solubility tests and peripheral blood film method for sickle cell disease screening at district health centers in Uganda, Clinics Mother Child Health 7(1).","DOI":"10.4303\/cmch\/C101947"},{"issue":"5","key":"10.1016\/j.saa.2022.121394_b0080","doi-asserted-by":"crossref","first-page":"519","DOI":"10.3390\/mi12050519","article-title":"Techniques for the detection of sickle cell disease: A review","volume":"12","author":"Arishi","year":"2021","journal-title":"Micromachines"},{"issue":"4","key":"10.1016\/j.saa.2022.121394_b0085","first-page":"538","article-title":"Abnormal haemoglobins: detection & characterization","volume":"134","author":"Wajcman","year":"2011","journal-title":"Indian J. Med. Res."},{"issue":"4","key":"10.1016\/j.saa.2022.121394_b0090","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3390\/ijns4040039","article-title":"Newborn screening for sickle cell disease and other hemoglobinopathies: a short review on classical laboratory methods\u2014isoelectric focusing, hplc, and capillary electrophoresis","volume":"4","author":"Fr\u00f6mmel","year":"2018","journal-title":"Int. J. Neonatal Screening"},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0095","doi-asserted-by":"crossref","first-page":"26","DOI":"10.29074\/ascls.25.1.26","article-title":"Novel test method (sickle confirm) to differentiate sickle cell anemia from sickle cell trait for potential use in developing countries","volume":"25","author":"Randolph","year":"2012","journal-title":"Am. Soc. Clin. Lab. Sci."},{"issue":"3","key":"10.1016\/j.saa.2022.121394_b0100","doi-asserted-by":"crossref","first-page":"427","DOI":"10.3390\/electronics9030427","article-title":"Deep learning models for classification of red blood cells in microscopy images to aid in sickle cell anemia diagnosis","volume":"9","author":"Alzubaidi","year":"2020","journal-title":"Electronics"},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12916-015-0473-6","article-title":"Validation of a novel point of care testing device for sickle cell disease","volume":"13","author":"Kanter","year":"2015","journal-title":"BMC Med."},{"key":"10.1016\/j.saa.2022.121394_b0110","series-title":"2018 International Conference on Advancement in Electrical and Electronic Engineering (ICAEEE), IEEE","first-page":"1","article-title":"Automatic sickle cell anemia detection using image processing technique","author":"Chy","year":"2018"},{"issue":"5","key":"10.1016\/j.saa.2022.121394_b0115","doi-asserted-by":"crossref","first-page":"e0177732","DOI":"10.1371\/journal.pone.0177732","article-title":"Towards a point-of-care strip test to diagnose sickle cell anemia","volume":"12","author":"Bond","year":"2017","journal-title":"PloS One"},{"issue":"12","key":"10.1016\/j.saa.2022.121394_b0120","doi-asserted-by":"crossref","first-page":"e114540","DOI":"10.1371\/journal.pone.0114540","article-title":"Evaluation of a density-based rapid diagnostic test for sickle cell disease in a clinical setting in zambia","volume":"9","author":"Kumar","year":"2014","journal-title":"PloS One"},{"issue":"8","key":"10.1016\/j.saa.2022.121394_b0125","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1039\/c3lc41302k","article-title":"A simple, rapid, low-cost diagnostic test for sickle cell disease","volume":"13","author":"Yang","year":"2013","journal-title":"Lab Chip"},{"issue":"4","key":"10.1016\/j.saa.2022.121394_b0130","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/bjh.14298","article-title":"A rapid, inexpensive and disposable point-of-care blood test for sickle cell disease using novel, highly specific monoclonal antibodies","volume":"175","author":"Quinn","year":"2016","journal-title":"Brit. J. Haematol."},{"key":"10.1016\/j.saa.2022.121394_b0135","series-title":"IEEE Biomedical Circuits and Systems Conference (BioCAS), IEEE","first-page":"1","article-title":"Live demonstration: Hemechip-a portable microchip electrophoresis technology for point-of-care sickle cell disease screening","author":"Hasan","year":"2018"},{"key":"10.1016\/j.saa.2022.121394_b0140","unstructured":"B.F. Kim, J. Bohandy, Spectroscopy of porphyrins, Johns Hopkins APL Technical Digest 2(3)."},{"issue":"35","key":"10.1016\/j.saa.2022.121394_b0145","doi-asserted-by":"crossref","first-page":"9921","DOI":"10.1021\/acs.jpcb.1c01684","article-title":"Molecular dynamics of hemoglobin reveals structural alterations and explains the interactions driving sickle cell fibrillation","volume":"125","author":"Maity","year":"2021","journal-title":"J. Phys. Chem. B"},{"issue":"3","key":"10.1016\/j.saa.2022.121394_b0150","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1006\/jmbi.1997.1253","article-title":"The high resolution crystal structure of deoxyhemoglobin s","volume":"272","author":"Harrington","year":"1997","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.saa.2022.121394_b0155","first-page":"24","article-title":"Haemoglobinopathy and sickle cell disease","volume":"10","author":"Wilson","year":"2010","journal-title":"Continuing Educ. Anaesthesia Crit. Care Pain"}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142522005431?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142522005431?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T02:12:46Z","timestamp":1761358366000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142522005431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":31,"alternative-id":["S1386142522005431"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2022.121394","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optical absorbance-based rapid test for the detection of sickle cell trait and sickle cell disease at the point-of-care","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2022.121394","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"121394"}}