{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T08:55:06Z","timestamp":1768726506591,"version":"3.49.0"},"reference-count":84,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.saa.2025.126272","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T19:00:12Z","timestamp":1745002812000},"page":"126272","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["On the behavior of Hoechst 33258 in DNA-PEG mixtures"],"prefix":"10.1016","volume":"339","author":[{"given":"Vladimir N.","family":"Morozov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail A.","family":"Klimovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Kolyvanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir A.","family":"Kuzmin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2025.126272_b0005","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s00418-013-1090-0","article-title":"Uptake and localisation of small-molecule fluorescent probes in living cells: a critical appraisal of QSAR models and a case study concerning probes for DNA and RNA","volume":"139","author":"Horobin","year":"2013","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.saa.2025.126272_b0010","doi-asserted-by":"crossref","first-page":"5515","DOI":"10.3390\/molecules26185515","article-title":"DNA dyes\u2014highly sensitive reporters of cell quantification: comparison with other cell quantification methods","volume":"26","author":"Ligasov\u00e1","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.saa.2025.126272_b0015","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0074-7696(08)60527-6","article-title":"Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area","volume":"192","author":"Luby-Phelps","year":"1999","journal-title":"Int. Rev. Cytol."},{"key":"10.1016\/j.saa.2025.126272_b0020","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S0968-0004(01)01938-7","article-title":"Macromolecular crowding: obvious but underappreciated","volume":"26","author":"Ellis","year":"2001","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.saa.2025.126272_b0025","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1083\/jcb.100.4.1091","article-title":"Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy","volume":"100","author":"Lanni","year":"1985","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.saa.2025.126272_b0030","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1021\/acs.analchem.8b04177","article-title":"Characterization of intracellular crowding environments with topology-based DNA quadruplex sensors","volume":"91","author":"Takahashi","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0035","series-title":"DNA liquid-Crystalline Dispersions and Nanoconstructions","author":"Yevdokimov","year":"2011"},{"key":"10.1016\/j.saa.2025.126272_b0040","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.tibs.2020.06.007","article-title":"Biomolecular condensates in the nucleus","volume":"45","author":"Sabari","year":"2020","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.saa.2025.126272_b0045","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/cyto.990050411","article-title":"Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Friend leukemia cells","volume":"5","author":"Darzynkiewicz","year":"1984","journal-title":"Cytometry A"},{"key":"10.1016\/j.saa.2025.126272_b0050","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1021\/bi00703a003","article-title":"Studies on the structure of deoxyribonucleoproteins. Spectroscopic characterization of the ethidium bromide binding sites","volume":"13","author":"Angerer","year":"1974","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0055","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1002\/cyto.a.20573","article-title":"Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells\u2013influence on chromatin organization and histone-DNA interactions","volume":"73","author":"Wojcik","year":"2008","journal-title":"Cytometry A"},{"key":"10.1016\/j.saa.2025.126272_b0060","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.1021\/ja211115q","article-title":"Protein crowding affects hydration structure and dynamics","volume":"134","author":"Harada","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.saa.2025.126272_b0065","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3389\/fchem.2019.00731","article-title":"Molecular basis of water activity in glycerol\u2013water mixtures","volume":"7","author":"Nakagawa","year":"2019","journal-title":"Front. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0070","article-title":"Short- and long-range crowding effects on water\u2019s hydrogen bond networks","volume":"2","author":"You","year":"2021","journal-title":"Cell Rep."},{"key":"10.1016\/j.saa.2025.126272_b0075","doi-asserted-by":"crossref","unstructured":"F.A. Goldbaum, F.P. Schwarz, E. Eisenstein, A. Cauerhff, R.A. Mariuzza, R.J. Poljak, The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1, J. Mol. Recogn. 9 (1996) 6\u201312. doi: 10.1002\/(sici)1099-1352(199601)9:1<6::aid-jmr240>3.0.co;2-v.","DOI":"10.1002\/(SICI)1099-1352(199601)9:1<6::AID-JMR240>3.3.CO;2-M"},{"key":"10.1016\/j.saa.2025.126272_b0080","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0141-8130(01)00127-1","article-title":"Effects of water activity and aqueous solvent ordering on thermal stability of lysozyme, alpha-chymotrypsinogen A, and alcohol dehydrogenase","volume":"28","author":"Matsue","year":"2001","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2025.126272_b0085","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/j.procbio.2014.08.009","article-title":"Influence of water availability on the enzymatic hydrolysis of proteins","volume":"49","author":"Butr\u00e9","year":"2014","journal-title":"Process Biochem."},{"key":"10.1016\/j.saa.2025.126272_b0090","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1021\/cr400113m","article-title":"Effects of molecular crowding on the structures, interactions, and functions of nucleic acids","volume":"114","author":"Nakano","year":"2014","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2025.126272_b0095","doi-asserted-by":"crossref","first-page":"23090","DOI":"10.3390\/ijms151223090","article-title":"What macromolecular crowding can do to a protein","volume":"15","author":"Kuznetsova","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.saa.2025.126272_b0100","doi-asserted-by":"crossref","DOI":"10.5402\/2013\/737805","article-title":"Macromolecular crowding enhances catalytic efficiency and stability of \u03b1-amylase","volume":"2013","author":"Yadav","year":"2013","journal-title":"ISRN Biotechnol."},{"key":"10.1016\/j.saa.2025.126272_b0105","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/j.febslet.2014.01.043","article-title":"Synthetic crowding agent dextran causes excluded volume interactions exclusively to tracer protein apoazurin","volume":"588","author":"Christiansen","year":"2014","journal-title":"FEBS Lett."},{"key":"10.1016\/j.saa.2025.126272_b0110","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1016\/j.bpj.2021.06.032","article-title":"Macromolecular crowding modulates \u03b1-synuclein amyloid fiber growth","volume":"120","author":"Horvath","year":"2021","journal-title":"Biophys. J."},{"key":"10.1016\/j.saa.2025.126272_b0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2020.114969","article-title":"The magnitude of macromolecular crowding caused by Dextran and Ficoll for the conformational stability of globular proteins","volume":"322","author":"Cozzolino","year":"2021","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2025.126272_b0120","doi-asserted-by":"crossref","first-page":"16614","DOI":"10.1021\/ja305300m","article-title":"Macromolecular crowding and protein stability","volume":"134","author":"Wang","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.saa.2025.126272_b0125","doi-asserted-by":"crossref","first-page":"4087","DOI":"10.1093\/nar\/gkv301","article-title":"Molecular crowding enhances facilitated diffusion of two human DNA glycosylases","volume":"43","author":"Cravens","year":"2015","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.saa.2025.126272_b0130","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1002\/bip.360350405","article-title":"The osmotic sensitivity of netropsin analogue binding to DNA","volume":"35","author":"Sidorova","year":"1995","journal-title":"Biopolymers"},{"key":"10.1016\/j.saa.2025.126272_b0135","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/ja002793v","article-title":"Hydration changes for DNA intercalation reactions","volume":"123","author":"Qu","year":"2001","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.saa.2025.126272_b0140","doi-asserted-by":"crossref","first-page":"16988","DOI":"10.1021\/bi051769x","article-title":"Hydration changes in the association of Hoechst 33258 with DNA","volume":"44","author":"Kiser","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0145","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1529\/biophysj.106.097451","article-title":"Hydration changes accompanying the binding of minor groove ligands with DNA","volume":"92","author":"Degtyareva","year":"2007","journal-title":"Biophys. J."},{"key":"10.1016\/j.saa.2025.126272_b0150","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1248\/cpb.58.1447","article-title":"Osmolyte changes the binding affinity and mode of interaction of minor groove binder Hoechst 33258 with calf thymus DNA","volume":"58","author":"Anuradha","year":"2010","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.saa.2025.126272_b0155","doi-asserted-by":"crossref","first-page":"3035","DOI":"10.1080\/07391102.2017.1375992","article-title":"Retained binding mode of various DNA-binding molecules under molecular crowding condition","volume":"36","author":"Oh","year":"2018","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.saa.2025.126272_b0160","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0378-4371(91)90436-G","article-title":"Ordered phases of DNA in vivo and in vitro","volume":"176","author":"Livolant","year":"1991","journal-title":"Phys. A"},{"key":"10.1016\/j.saa.2025.126272_b0165","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/newbio236067a0","article-title":"Structure and circular dichroism of DNA in concentrated polymer solutions","volume":"236","author":"Jordan","year":"1972","journal-title":"Nat. New Biol."},{"key":"10.1016\/j.saa.2025.126272_b0170","doi-asserted-by":"crossref","first-page":"6595","DOI":"10.1063\/1.469375","article-title":"Collapse of single DNA molecule in poly(ethylene glycol) solutions","volume":"102","author":"Vasilevskaya","year":"1995","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.saa.2025.126272_b0175","doi-asserted-by":"crossref","first-page":"947","DOI":"10.3367\/UFNe.2020.09.038843","article-title":"Multiplicity of 're-entrant' cholesteric structures in DNA liquid-crystalline dispersions","volume":"64","author":"Yevdokimov","year":"2021","journal-title":"Phys. Usp."},{"key":"10.1016\/j.saa.2025.126272_b0180","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/S0079-6700(96)00016-0","article-title":"Condensed phases of DNA: structures and phase transitions","volume":"21","author":"Livolant","year":"1996","journal-title":"Prog. Polym. Sci."},{"key":"10.1016\/j.saa.2025.126272_b0185","doi-asserted-by":"crossref","first-page":"7363","DOI":"10.1073\/pnas.95.13.7363","article-title":"In disperse solution, \u201cosmotic stress\u201d is a restricted case of preferential interactions","volume":"95","author":"Timasheff","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.saa.2025.126272_b0190","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1002\/chir.530030404","article-title":"The effect of ethidium bromide on the liquid crystalline phases of aqueous DNA","volume":"3","author":"Gottarelli","year":"1991","journal-title":"Chirality"},{"key":"10.1016\/j.saa.2025.126272_b0195","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1039\/b211671e","article-title":"Optical microscopy and multinuclear NMR investigation of the liquid crystalline netropsin\u2013DNA complex","volume":"5","author":"Cesare-Marincola","year":"2003","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.saa.2025.126272_b0200","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1295\/polymj.PJ2005210","article-title":"The EB-DNA liquid crystalline complex with high concentration of Mg2+","volume":"38","author":"Liao","year":"2006","journal-title":"Polym. J."},{"key":"10.1016\/j.saa.2025.126272_b0205","doi-asserted-by":"crossref","first-page":"7183","DOI":"10.1039\/D0OB01723J","article-title":"Guanine damage by singlet oxygen from SYBR Green I in liquid crystalline DNA","volume":"18","author":"Sakurai","year":"2020","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0210","doi-asserted-by":"crossref","DOI":"10.1016\/j.jlumin.2022.119381","article-title":"F\u00f6rster resonance energy transfer from Hoechst 33258 to SYBR Green I in cholesteric liquid-crystalline DNA","volume":"252","author":"Morozov","year":"2022","journal-title":"J. Lumin."},{"key":"10.1016\/j.saa.2025.126272_b0215","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3390\/chemosensors6020018","article-title":"The use of Hoechst dyes for DNA staining and beyond","volume":"6","author":"Bucevi\u010dius","year":"2018","journal-title":"Chemosensors"},{"key":"10.1016\/j.saa.2025.126272_b0220","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1080\/09553009214550641","article-title":"DNA ligands as radioprotectors: molecular studies with Hoechst 33342 and Hoechst 33258","volume":"61","author":"Denison","year":"1992","journal-title":"Int. J. Radiat. Biol."},{"key":"10.1016\/j.saa.2025.126272_b0225","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1134\/S0018143924010107","article-title":"On a novel approach to investigation and evaluation of the efficacy of DNA-binding radioprotectors","volume":"58","author":"Kolyvanova","year":"2024","journal-title":"High Energy Chem."},{"key":"10.1016\/j.saa.2025.126272_b0230","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3390\/photonics10060671","article-title":"Chemical dosimetry using bisbenzimidazoles: solvent-dependent fluorescence response of Hoechst 33258 to radiation exposure","volume":"10","author":"Kolyvanova","year":"2023","journal-title":"Photonics"},{"key":"10.1016\/j.saa.2025.126272_b0235","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/BF00364015","article-title":"Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes","volume":"52","author":"Latt","year":"1975","journal-title":"Chromosoma"},{"key":"10.1016\/j.saa.2025.126272_b0240","doi-asserted-by":"crossref","first-page":"7336","DOI":"10.1021\/jp066344e","article-title":"Multiple binding modes for dicationic Hoechst 33258 to DNA","volume":"111","author":"Guan","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.saa.2025.126272_b0245","doi-asserted-by":"crossref","first-page":"9029","DOI":"10.1021\/bi00490a021","article-title":"Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)], and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities","volume":"29","author":"Loontiens","year":"1990","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0250","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF00492587","article-title":"Accurate determination of DNA content in single cell nuclei stained with Hoechst 33258 fluorochrome at high salt concentration","volume":"87","author":"Araki","year":"1987","journal-title":"Histochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0255","doi-asserted-by":"crossref","first-page":"42041","DOI":"10.1074\/jbc.M406053200","article-title":"Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared and capillary electrophoresis","volume":"279","author":"Ouameur","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0260","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1080\/07391102.2009.10507283","article-title":"Binding of Hoechst 33258 and its derivatives to DNA","volume":"26","author":"Bazhulina","year":"2009","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.saa.2025.126272_b0265","doi-asserted-by":"crossref","first-page":"e103","DOI":"10.1093\/nar\/gnh101","article-title":"Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications","volume":"32","author":"Zipper","year":"2004","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.saa.2025.126272_b0270","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1070\/PU2004v047n06ABEH001828","article-title":"Portable optical biosensors for the determination of biologically active and toxic compounds","volume":"47","author":"Kompanets","year":"2004","journal-title":"Phys. Usp."},{"key":"10.1016\/j.saa.2025.126272_b0275","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.2174\/092986707781389673","article-title":"DNA minor groove binders: an overview on molecular modeling and QSAR approaches","volume":"14","author":"Lauria","year":"2007","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0280","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jphotobiol.2012.07.003","article-title":"Chemistry of DNA minor groove binding agents","volume":"115","author":"Khan","year":"2012","journal-title":"J. Photochem. Photobiol. B"},{"key":"10.1016\/j.saa.2025.126272_b0285","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0022-2836(87)90123-9","article-title":"Binding of Hoechst 33258 to the minor groove of B-DNA","volume":"197","author":"Pjura","year":"1987","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.saa.2025.126272_b0290","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1093\/nar\/16.6.2671","article-title":"The molecular structure of the complex of Hoechst 33258 and the DNA dodecamer d(CGCGAATTCGCG)","volume":"16","author":"Teng","year":"1988","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.saa.2025.126272_b0295","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1093\/nar\/18.13.3753","article-title":"Sequence-specific interaction of Hoechst 33258 with the minor groove of an adenine-tract DNA duplex studied in solution by 1H NMR spectroscopy","volume":"18","author":"Searle","year":"1990","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.saa.2025.126272_b0300","doi-asserted-by":"crossref","first-page":"11377","DOI":"10.1021\/bi00112a004","article-title":"Dynamics and binding mode of Hoechst 33258 to d(GTGGAATTCCAC)2 in the 1:1 solution complex as determined by two-dimensional 1H NMR","volume":"30","author":"Fede","year":"1991","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0305","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1177\/33.4.2579998","article-title":"Multiple binding modes for Hoechst 33258 to DNA","volume":"33","author":"Stokke","year":"1985","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.saa.2025.126272_b0310","doi-asserted-by":"crossref","first-page":"5820","DOI":"10.1021\/jp400418w","article-title":"Minor-groove binding drugs: where is the second Hoechst 33258 molecule?","volume":"117","author":"Fornander","year":"2013","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.saa.2025.126272_b0315","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1751-1097.1990.tb01686.x","article-title":"A fluorescence study of the binding of Hoechst 33258 and DAPI to halogenated DNAs","volume":"51","author":"H\u00e4rd","year":"1990","journal-title":"Photochem. Photobiol."},{"key":"10.1016\/j.saa.2025.126272_b0320","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1070\/RC2011v080n12ABEH004246","article-title":"Molecular rotors as luminescence sensors of local viscosity and viscous flow in solutions and organized systems","volume":"80","author":"Uzhinov","year":"2011","journal-title":"Russ. Chem. Rev."},{"key":"10.1016\/j.saa.2025.126272_b0325","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.colsurfa.2006.12.069","article-title":"Aggregation of azo dye Orange I induced by polyethylene glycol in aqueous solution","volume":"301","author":"Ouyang","year":"2007","journal-title":"Colloids Surf. A"},{"key":"10.1016\/j.saa.2025.126272_b0330","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.jmb.2008.05.073","article-title":"Calorimetric and spectroscopic studies of Hoechst 33258: self-association and binding to non-cognate DNA","volume":"381","author":"Buurma","year":"2008","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.saa.2025.126272_b0335","doi-asserted-by":"crossref","first-page":"4575","DOI":"10.1021\/jp512306c","article-title":"Aggregation features and fluorescence of Hoechst 33258","volume":"119","author":"Busto","year":"2015","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.saa.2025.126272_b0340","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.aca.2006.03.106","article-title":"Determination of nucleic acids based on the fluorescence quenching of Hoechst 33258 at pH 4.5","volume":"570","author":"Guan","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.saa.2025.126272_b0345","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1006\/jmbi.1997.1170","article-title":"Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies","volume":"271","author":"Haq","year":"1997","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.saa.2025.126272_b0350","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF00495018","article-title":"NaCl-aided Hoechst 33258 staining method for DNA quantification and its application","volume":"92","author":"Yamamoto","year":"1989","journal-title":"Histochemistry"},{"key":"10.1016\/j.saa.2025.126272_b0355","doi-asserted-by":"crossref","first-page":"11365","DOI":"10.3390\/ijms241411365","article-title":"Optical polymorphism of liquid\u2013crystalline dispersions of DNA at high concentrations of crowding polymer","volume":"24","author":"Morozov","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.saa.2025.126272_b0360","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1134\/S1061933X24600234","article-title":"Interaction of ultrasmall gold nanoparticles with liquid-crystalline DNA microparticles: destruction vs stabilization","volume":"86","author":"Kolyvanova","year":"2024","journal-title":"Colloid J."},{"key":"10.1016\/j.saa.2025.126272_b0365","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1002\/chir.10220","article-title":"Induced circular dichroism by chiral molecular interaction","volume":"15","author":"Allenmark","year":"2003","journal-title":"Chirality"},{"key":"10.1016\/j.saa.2025.126272_b0370","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1080\/07391102.1985.10506319","article-title":"Two binding modes of netropsin are involved in the complex formation with poly(dA-dT).poly(dA-dT) and other alternating DNA duplex polymers","volume":"2","author":"Burckhardt","year":"1985","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.saa.2025.126272_b0375","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0009-2797(91)90078-L","article-title":"The origin of the DNA induced circular dichroism of CC-1065 and analogs","volume":"79","author":"Krueger","year":"1991","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.saa.2025.126272_b0380","doi-asserted-by":"crossref","first-page":"14947","DOI":"10.1021\/jacs.7b05994","article-title":"Origin of DNA-induced circular dichroism in a minor-groove binder","volume":"139","author":"List","year":"2017","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.saa.2025.126272_b0385","doi-asserted-by":"crossref","first-page":"2972","DOI":"10.1063\/1.450278","article-title":"Theory of the interaction of light with large inhomogeneous molecular aggregates. II. Psi\u2010type circular dichroism","volume":"84","author":"Keller","year":"1986","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.saa.2025.126272_b0390","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1134\/S0006350915050036","article-title":"About the spatial organization of double-stranded DNA molecules in the cholesteric liquid-crystalline phase and dispersion particles of this phase","volume":"60","author":"Yevdokimov","year":"2015","journal-title":"Biophysics"},{"key":"10.1016\/j.saa.2025.126272_b0395","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1562\/0031-8655(2001)073<0585:PPOFDD>2.0.CO;2","article-title":"Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution","volume":"73","author":"Cosa","year":"2001","journal-title":"Photochem. Photobiol."},{"key":"10.1016\/j.saa.2025.126272_b0400","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s10895-012-1059-8","article-title":"SYBR Green I: fluorescence properties and interaction with DNA","volume":"22","author":"Dragan","year":"2012","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.saa.2025.126272_b0405","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1073\/pnas.98.4.1999","article-title":"Hyperosmolality in the form of elevated NaCl but not urea causes DNA damage in murine kidney cells","volume":"98","author":"K\u00fcltz","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.saa.2025.126272_b0410","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.comptc.2011.09.010","article-title":"Structure and hydrogen bonding in aqueous sodium chloride solutions using theoretical water model AB4: effects of concentration","volume":"977","author":"Beladjine","year":"2011","journal-title":"Comput. Theor. Chem."},{"key":"10.1016\/j.saa.2025.126272_b0415","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.chroma.2015.11.019","article-title":"Effect of sodium chloride on solute\u2013solvent interactions in aqueous polyethylene glycol\u2013sodium sulfate two-phase systems","volume":"1425","author":"da Silva","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.saa.2025.126272_b0420","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1016\/S0006-3495(99)77023-2","article-title":"Sodium and chlorine ions as part of the DNA solvation shell","volume":"77","author":"Feig","year":"1999","journal-title":"Biophys. J."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142525005785?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142525005785?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:32:45Z","timestamp":1762543965000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142525005785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":84,"alternative-id":["S1386142525005785"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2025.126272","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"On the behavior of Hoechst 33258 in DNA-PEG mixtures","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2025.126272","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"126272"}}