{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:21:34Z","timestamp":1783045294865,"version":"3.54.6"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Hebei Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["B2024201026"],"award-info":[{"award-number":["B2024201026"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.saa.2026.128042","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:10:29Z","timestamp":1778109029000},"page":"128042","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Dopamine-mediated signal amplification integrated with CCK-8 detection for highly sensitive colorimetric immunoassay"],"prefix":"10.1016","volume":"360","author":[{"given":"Nan","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaoyuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2026.128042_bb0005","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1021\/acs.chemrev.8b00257","article-title":"Highly sensitive and multiplexed protein measurements","volume":"119","author":"Cohen","year":"2019","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2026.128042_bb0010","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1039\/D3SD00144J","article-title":"Recent progress in digital immunoassay: how to achieve ultrasensitive, multiplex and clinical accessible detection","volume":"3","author":"Zhang","year":"2024","journal-title":"Sens. Diagn."},{"key":"10.1016\/j.saa.2026.128042_bb0015","doi-asserted-by":"crossref","first-page":"8385","DOI":"10.1021\/acs.analchem.4c07031","article-title":"Single-molecule liquid biopsy detects low- and high-abundance protein markers simultaneously for pancreatic cancer diagnosis","volume":"97","author":"Wang","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0020","doi-asserted-by":"crossref","first-page":"10947","DOI":"10.1021\/acs.analchem.5c02358","article-title":"A bimetallic nanozyme synergistic effect-driven enzyme cascade nanoreactor for instant immunoassay","volume":"97","author":"Wu","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2025.118604","article-title":"Recent advances in single-molecule immunoassays and their clinical applications","volume":"195","author":"Li","year":"2026","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0030","article-title":"Signal amplification strategy design in nanozyme-based biosensors for highly sensitive detection of trace biomarkers","volume":"7","author":"Wang","year":"2023","journal-title":"Small Methods"},{"key":"10.1016\/j.saa.2026.128042_bb0035","doi-asserted-by":"crossref","first-page":"5512","DOI":"10.1021\/acsabm.5c00738","article-title":"Plasmonic ELISA for biomarker detection: a review of mechanisms, functionalization strategies, and emerging modalities","volume":"8","author":"Shoukat","year":"2025","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.saa.2026.128042_bb0040","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1021\/acsnano.1c08675","article-title":"High-throughput, high-multiplex digital protein detection with attomolar sensitivity","volume":"16","author":"Wu","year":"2022","journal-title":"ACS Nano"},{"key":"10.1016\/j.saa.2026.128042_bb0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117502","article-title":"Digital platforms enabling single-molecule analysis for cancer detection","volume":"171","author":"Li","year":"2024","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112349","article-title":"Diagnostic methods and potential portable biosensors for coronavirus disease 2019","volume":"165","author":"Cui","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128042_bb0055","doi-asserted-by":"crossref","first-page":"17109","DOI":"10.1021\/acsami.3c18304","article-title":"Sandwich immuno-RCA assay with single molecule counting readout: the importance of biointerface design","volume":"16","author":"Schmidt","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.saa.2026.128042_bb0060","doi-asserted-by":"crossref","first-page":"22692","DOI":"10.1021\/acs.analchem.5c04062","article-title":"IDSP-IA: Immunotriggered double stem-loop probe-mediated isothermal amplification for attomolar protein quantification","volume":"97","author":"Zhang","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0065","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s13206-024-00165-z","article-title":"Development of multi-HRP-conjugated branched PEI\/antibody-functionalized gold nanoparticles for ultra-sensitive ELISA","volume":"18","author":"Shin","year":"2024","journal-title":"Biochip J."},{"key":"10.1016\/j.saa.2026.128042_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2025.127715","article-title":"Multifunctional dopamine-modified conjugated polymer nanoparticles for ultrasensitive immunoassays","volume":"288","author":"Wang","year":"2025","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128042_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2024.111602","article-title":"Recent advances of nanozyme-enhanced lateral flow assay sensing in clinic diagnosis","volume":"206","author":"Xiong","year":"2024","journal-title":"Microchem. J."},{"key":"10.1016\/j.saa.2026.128042_bb0080","doi-asserted-by":"crossref","DOI":"10.3389\/fonc.2024.1473414","article-title":"Research progress and perspectives on the application of tyramide signal amplification-based multiplex immunohistochemistry\/immunofluorescence: a bibliometrics analysis","volume":"14","author":"Yu","year":"2025","journal-title":"Front. Oncol."},{"key":"10.1016\/j.saa.2026.128042_bb0085","doi-asserted-by":"crossref","first-page":"21922","DOI":"10.1038\/s41598-023-48959-9","article-title":"Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging","volume":"13","author":"Wang","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.saa.2026.128042_bb0090","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3390\/cancers12020255","article-title":"Procedural requirements and recommendations for multiplex immunofluorescence tyramide signal amplification assays to support translational oncology studies","volume":"12","author":"Parra","year":"2020","journal-title":"Cancers"},{"key":"10.1016\/j.saa.2026.128042_bb0095","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1021\/acs.analchem.3c05140","article-title":"Tyramine-invertase bioconjugate-amplified personal glucose meter signaling for ultrasensitive immunoassay","volume":"96","author":"Dai","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0100","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.talanta.2018.01.068","article-title":"Synergistic electron transfer effect-based signal amplification strategy for the ultrasensitive detection of dopamine","volume":"182","author":"Lu","year":"2018","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128042_bb0105","doi-asserted-by":"crossref","first-page":"8506","DOI":"10.1039\/C7TB02291C","article-title":"In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO 2 nanoflakes for a sensitive immunoassay of cancer biomarkers","volume":"5","author":"Lin","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.saa.2026.128042_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2022.129257","article-title":"A universal, portable, and ultra-sensitive pipet immunoassay platform for deoxynivalenol detection based on dopamine self-polymerization-mediated bioconjugation and signal amplification","volume":"436","author":"Hong","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.saa.2026.128042_bb0115","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1039\/C5TB02525G","article-title":"In situ generation of electron acceptor to amplify the photoelectrochemical signal from poly(dopamine)-sensitized TiO2 signal crystal for immunoassay","volume":"4","author":"Li","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.saa.2026.128042_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.yofte.2023.103468","article-title":"A polydopamine nanospheres modified fiber optic SPR biosensor for specific detection of C-reactive protein","volume":"80","author":"Cao","year":"2023","journal-title":"Opt. Fiber Technol."},{"key":"10.1016\/j.saa.2026.128042_bb0125","doi-asserted-by":"crossref","DOI":"10.1038\/s41551-017-0082","article-title":"Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine","volume":"1","author":"Li","year":"2017","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.saa.2026.128042_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116836","article-title":"New sensing methods using commercially available products: based on PGM and PTS","volume":"267","author":"Jiang","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128042_bb0135","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3389\/fmolb.2020.00137","article-title":"VASH-1 regulates oxidative stress and fibrosis in diabetic kidney disease via SIRT1\/HIF1\u03b1 and TGF\u03b21\/Smad3 signaling pathways","volume":"7","author":"Ren","year":"2020","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.saa.2026.128042_bb0140","doi-asserted-by":"crossref","first-page":"343","DOI":"10.3390\/pathogens12020343","article-title":"Potential application of the WST-8-mPMS assay for rapid viable microorganism detection","volume":"12","author":"Chen","year":"2023","journal-title":"Pathogens"},{"key":"10.1016\/j.saa.2026.128042_bb0145","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1021\/acs.chemrestox.3c00339","article-title":"Cell via cell viability assay changes cellular metabolic characteristics by intervening with glycolysis and pentose phosphate pathway","volume":"37","author":"Fan","year":"2024","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.saa.2026.128042_bb0150","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0022-1759(99)00166-0","article-title":"A stable and highly sensitive 3,3\u2032,5,5\u2032-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays","volume":"233","author":"Frey","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.saa.2026.128042_bb0155","doi-asserted-by":"crossref","first-page":"7372","DOI":"10.1039\/D4AY01480D","article-title":"A dual-mode homogeneous electrochemical-colorimetric biosensing sensor for carcinoembryonic antigen detection based on a microfluidic paper-based analysis device","volume":"16","author":"Zhang","year":"2024","journal-title":"Anal. Methods"},{"key":"10.1016\/j.saa.2026.128042_bb0160","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1021\/acssensors.1c02025","article-title":"Bimodal multiplexed detection of tumor markers in non-small cell lung cancer with polymer dot-based immunoassay","volume":"6","author":"Yang","year":"2021","journal-title":"ACS Sens."},{"key":"10.1016\/j.saa.2026.128042_bb0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.124414","article-title":"Novel rapid coordination of ascorbic acid 2-phosphate and iron(III) as chromogenic substrate system based on Fe2O3 nanoparticle and application in immunoassay for the colorimetric detection of carcinoembryonic antigen","volume":"258","author":"Chen","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128042_bb0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2024.123008","article-title":"Vanadium single-atoms coordinated artificial peroxidases as biocatalyst-linked immunosorbent assay for highly-sensitive carcinoembryonic antigen immunoassay","volume":"316","author":"Yuan","year":"2025","journal-title":"Biomaterials"},{"key":"10.1016\/j.saa.2026.128042_bb0175","doi-asserted-by":"crossref","first-page":"16942","DOI":"10.1021\/acs.analchem.5c02403","article-title":"Dual-enhanced lateral flow immunoassay: synergizing oriented antibody anchoring and nanozyme catalytic amplification for ultrasensitive cancer detection","volume":"97","author":"Yang","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2023.115370","article-title":"Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection","volume":"683","author":"Xiang","year":"2023","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.saa.2026.128042_bb0185","doi-asserted-by":"crossref","first-page":"13558","DOI":"10.1039\/C8NJ02038H","article-title":"A metal\u2013organic framework nanomaterial as an ideal loading platform for ultrasensitive electrochemiluminescence immunoassays","volume":"42","author":"Huang","year":"2018","journal-title":"New J. Chem."},{"key":"10.1016\/j.saa.2026.128042_bb0190","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202000540","article-title":"Nature-inspired adhesive catecholamines for highly concentrated colorimetric signal in spatial biomarker labeling","volume":"9","author":"Kim","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.saa.2026.128042_bb0195","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1021\/acs.nanolett.5c05256","article-title":"HepatoAILFA: machine-learning-assisted nano-enhanced point-of-care system for personalized precise diagnosis of hepatocellular carcinoma","volume":"26","author":"Zuo","year":"2025","journal-title":"Nano Lett."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S138614252600613X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S138614252600613X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:01:32Z","timestamp":1783044092000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S138614252600613X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":39,"alternative-id":["S138614252600613X"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2026.128042","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dopamine-mediated signal amplification integrated with CCK-8 detection for highly sensitive colorimetric immunoassay","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2026.128042","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128042"}}