{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T22:15:09Z","timestamp":1784240109231,"version":"3.55.0"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2025MS217"],"award-info":[{"award-number":["ZR2025MS217"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.saa.2026.128104","type":"journal-article","created":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:41:29Z","timestamp":1779583289000},"page":"128104","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Explore the interaction mechanism between artificial synthetic food colorants and digestive proteases via multi-spectral technology and computer simulation"],"prefix":"10.1016","volume":"361","author":[{"given":"Ruofei","family":"Du","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2026.128104_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2024.141925","article-title":"Food additives as photosensitizers: a systematic review and narrative synthesis","volume":"464","author":"Dai","year":"2025","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jfp.2025.100607","article-title":"Progress and challenge in the risk management of food additives","volume":"88","author":"Niu","year":"2025","journal-title":"J. Food Protect."},{"key":"10.1016\/j.saa.2026.128104_bb0015","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1021\/acs.jafc.1c07533","article-title":"Natural food colorants and preservatives: a review, a demand, and a challenge","volume":"70","author":"Novais","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0020","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.foodchem.2014.08.088","article-title":"Characterisation of interaction between food colourant allura red AC and human serum albumin: multispectroscopic analyses and docking simulations","volume":"170","author":"Wu","year":"2015","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0025","doi-asserted-by":"crossref","first-page":"8497","DOI":"10.1080\/10408398.2021.1929823","article-title":"Food additives: from functions to analytical methods","volume":"62","author":"Wu","year":"2022","journal-title":"Crit. Rev. Food Sci."},{"key":"10.1016\/j.saa.2026.128104_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2021.117838","article-title":"Molecular interaction of sunset yellow with whey protein: multi-spectroscopic techniques and computational study","volume":"345","author":"Khan","year":"2022","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2021.116715","article-title":"Evaluation of interactions between food colorant, tartrazine, and apo-transferrin using spectroscopic analysis and docking simulation","volume":"339","author":"Jafari-Arvari","year":"2021","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0040","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ijbiomac.2021.12.121","article-title":"The inhibitory effect of sunset yellow on thermally induced human serum albumin aggregates: possible role in naturopathy","volume":"199","author":"Zakariya","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0045","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1136\/archdischild-2023-326565","article-title":"Artificial food additives: hazardous to long-term health?","volume":"109","author":"Warner","year":"2024","journal-title":"Arch. Dis. Child."},{"key":"10.1016\/j.saa.2026.128104_bb0050","first-page":"1","article-title":"Food additives: over-view of related safety concerns","volume":"5","author":"Sunday","year":"2022","journal-title":"Food Sci. Nutr."},{"key":"10.1016\/j.saa.2026.128104_bb0055","article-title":"Toxicological and teratogenic effect of various food additives: an updated review","volume":"24","author":"Sambu","year":"2022","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.saa.2026.128104_bb0060","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1080\/1028415X.2024.2433257","article-title":"Food additives for the central nervous system, useful or harmful? An evidence-based review","volume":"28","author":"Fadaei","year":"2025","journal-title":"Nutr. Neurosci."},{"key":"10.1016\/j.saa.2026.128104_bb0065","article-title":"Ponceau 4R induces aggregation in human serum albumin and morin acts as an anti-aggregating agent against dye induced aggregates","volume":"335","author":"Fatima","year":"2024","journal-title":"Spectrochimi. Acta A"},{"key":"10.1016\/j.saa.2026.128104_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2025.127133","article-title":"Amyloid fibril formation in human serum albumin on interaction with indigo carmine","volume":"424","author":"James","year":"2025","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0075","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1080\/07391102.2023.2289046","article-title":"Aggregation and cytotoxicity of food additive dye (azorubine)-albumin adducts: a multi-spectroscopic, microscopic and computational analysis","volume":"43","author":"Khan","year":"2025","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.saa.2026.128104_bb0080","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.compbiolchem.2015.04.006","article-title":"Study on the interaction of artificial and natural food colorants with human serum albumin: a computational point of view","volume":"56","author":"Masone","year":"2015","journal-title":"Comput. Biol. Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0085","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.foodchem.2012.09.026","article-title":"Mechanistic and conformational studies on the interaction of food dye amaranth with human serum albumin by multispectroscopic methods","volume":"136","author":"Zhang","year":"2013","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0090","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1038\/s41598-018-38240-9","article-title":"Computational and spectroscopic analysis of interaction between food colorant citrus red 2 and human serum albumin","volume":"9","author":"Wu","year":"2019","journal-title":"Sci. Rep-UK."},{"key":"10.1016\/j.saa.2026.128104_bb0095","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1021\/jp407057f","article-title":"Exploration of pH-dependent behavior of the anion receptor pocket of subdomain IIA of HSA: determination of effective pocket charge using the debye\u2212h\u00fcckel limiting law","volume":"118","author":"Bolel","year":"2014","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.saa.2026.128104_bb0100","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.foodchem.2014.11.141","article-title":"Thermodynamics of the interaction of the food additive tartrazine with serum albumins: a microcalorimetric investigation","volume":"175","author":"Basu","year":"2015","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0105","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.saa.2017.09.062","article-title":"Unveiling the stimulatory effects of tartrazine on human and bovine serum albumin fibrillogenesis: spectroscopic and microscopic study","volume":"191","author":"Al-Shabib","year":"2018","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.saa.2026.128104_bb0110","doi-asserted-by":"crossref","first-page":"10195","DOI":"10.1021\/jp304537m","article-title":"Spectroscopic investigation of the effect of salt on binding of tartrazine with two homologous serum albumins: quantification by use of the debye\u2212h\u00fcckel limiting law and observation of enthalpy\u2212entropy compensation","volume":"116","author":"Bolel","year":"2012","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.saa.2026.128104_bb0115","doi-asserted-by":"crossref","first-page":"6650","DOI":"10.1021\/jf200907x","article-title":"Characterizing the interaction between tartrazine and two serum albumins by a hybrid spectroscopic approach","volume":"59","author":"Pan","year":"2011","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0120","doi-asserted-by":"crossref","first-page":"3727","DOI":"10.1021\/jf205219w","article-title":"Optical spectroscopic exploration of binding of cochineal red a with two homologous serum albumins","volume":"60","author":"Bolel","year":"2012","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.129143","article-title":"Interaction of food colorant indigo carmine with human and bovine serum albumins: a multispectroscopic, calorimetric, and theoretical investigation","volume":"259","author":"Jana","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0130","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.talanta.2005.03.027","article-title":"A rapid and sensitive method for the determination of trace proteins based on the interaction between proteins and ponceau 4R","volume":"67","author":"Zhong","year":"2005","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128104_bb0135","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1080\/10934529.2020.1729616","article-title":"Investigation on the interaction of food colorant Sudan III with bovine serum albumin using spectroscopic and molecular docking methods","volume":"55","author":"Bai","year":"2020","journal-title":"J. Environ. Sci. Heal. A"},{"key":"10.1016\/j.saa.2026.128104_bb0140","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1016\/j.foodchem.2012.06.095","article-title":"Study on the interaction of food colourant quinoline yellow with bovine serum albumin by spectroscopic techniques","volume":"135","author":"Shahabadi","year":"2012","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0145","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.foodchem.2016.05.053","article-title":"Interactions of two food colourants with BSA: analysis by debye-h\u00fcckel theory","volume":"211","author":"Li","year":"2016","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0150","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s00706-011-0593-4","article-title":"Effects of synthetic food colorants on the interaction between norfloxacin and bovine serum albumin by fluorescence spectroscopy","volume":"143","author":"Liu","year":"2012","journal-title":"Monatsh. Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0155","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/137531","article-title":"Investigation on the competition interaction of synthetic food colorants and ciprofloxacin hydrochloride with bovine serum albumin by fluorescence spectroscopy","volume":"2011","author":"Liu","year":"2011","journal-title":"J. Thermodyn."},{"key":"10.1016\/j.saa.2026.128104_bb0160","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.jhazmat.2015.02.044","article-title":"Interaction of toxic azo dyes with heme protein: biophysical insights into the binding aspect of the food additive amaranth with human hemoglobin","volume":"289","author":"Basu","year":"2015","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.saa.2026.128104_bb0165","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.jhazmat.2016.07.023","article-title":"Multispectroscopic and calorimetric studies on the binding of the food colorant tartrazine with human hemoglobin","volume":"318","author":"Basu","year":"2016","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.saa.2026.128104_bb0170","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.foodchem.2016.06.078","article-title":"Degradation products of the artificial azo dye, allura red, inhibit esterase activity of carbonic anhydrase II: a basic in vitro study on the food safety of the colorant in terms of enzyme inhibition","volume":"213","author":"Esmaeili","year":"2016","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2023.135826","article-title":"Comparative analysis of the interaction between alpha-lactalbumin and two edible azo colorants equipped with different sulfonyl group numbers","volume":"416","author":"Li","year":"2023","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0180","doi-asserted-by":"crossref","first-page":"11579","DOI":"10.1021\/acs.langmuir.9b01242","article-title":"Curious results in the prospective binding interactions of the food additive tartrazine with \u03b2-lactoglobulin","volume":"35","author":"Patel","year":"2019","journal-title":"Langmuir"},{"key":"10.1016\/j.saa.2026.128104_bb0185","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.foodchem.2019.01.103","article-title":"Comparative analysis of the interaction of mono-, dis-, and tris-azo food dyes with egg white lysozyme: a combined spectroscopic and computational simulation approach","volume":"284","author":"Wu","year":"2019","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128104_bb0190","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.ijbiomac.2022.08.199","article-title":"Role of salts and solvents on the defibrillation of food dye \u201csunset yellow\u201d induced hen egg white lysozyme amyloid fibrils","volume":"219","author":"Hakeem","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0195","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ijbiomac.2019.01.049","article-title":"Allura red rapidly induces amyloid-like fibril formation in hen egg white lysozyme at physiological pH","volume":"127","author":"Al-Shabib","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0200","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ijbiomac.2018.04.080","article-title":"Spectroscopy and molecular dynamics simulation study on the interaction of sunset yellow food additive with pepsin","volume":"115","author":"Mohseni-Shahri","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0205","doi-asserted-by":"crossref","first-page":"58","DOI":"10.4103\/1735-5362.305189","article-title":"Interactions and effects of food additive dye allura red on pepsin structure and protease activity; experimental and computational supports","volume":"16","author":"Balaei","year":"2020","journal-title":"Res. Pharm. Sci."},{"key":"10.1016\/j.saa.2026.128104_bb0210","article-title":"Evaluation of interaction between ponceau 4R (P4R) and trypsin using kinetic, spectroscopic, and molecular dynamics simulation methods","volume":"362","author":"Mostafavi","year":"2022","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0215","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2023.122702","article-title":"Effects of two food colorants on catalase and trypsin: binding evidences from experimental and computational analysis","volume":"297","author":"Pan","year":"2023","journal-title":"Spectrochimi. Acta A"},{"key":"10.1016\/j.saa.2026.128104_bb0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.130442","article-title":"Probing the interaction mechanisms between sunset yellow dye and trypsin protein leading to amorphous aggregation under low pH conditions","volume":"265","author":"Al-Shabib","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.saa.2026.128104_bb0225","article-title":"The interaction between the azo dye Tartrazine and a \u2013chymotrypsin enzyme: molecular dynamics simulation and multi-spectroscopic investigations","volume":"344","author":"Dehkordi","year":"2021","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0230","series-title":"Principles of Fluorescence Spectroscopy","author":"Lakowicz","year":"2006"},{"key":"10.1016\/j.saa.2026.128104_bb0235","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2021.119859","article-title":"Exploring the interactions of naringenin and naringin with trypsin and pepsin: experimental and computational modeling approaches","volume":"258","author":"Li","year":"2021","journal-title":"Spectrochimi. Acta A"},{"key":"10.1016\/j.saa.2026.128104_bb0240","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.molstruc.2004.05.026","article-title":"Investigation of the interaction between flavonoids and human serum albumin","volume":"703","author":"Bi","year":"2004","journal-title":"J. Mol. Struct."},{"key":"10.1016\/j.saa.2026.128104_bb0245","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.saa.2004.05.028","article-title":"Molecular spectroscopic study on the interaction of tetracyclines with serum albumins","volume":"61","author":"Bi","year":"2005","journal-title":"Spectrochimi. Acta A"},{"key":"10.1016\/j.saa.2026.128104_bb0250","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1021\/bi00514a017","article-title":"Thermodynamics of protein association reactions: forces contributing to stability","volume":"20","author":"Ross","year":"1981","journal-title":"Biochemistry"},{"key":"10.1016\/j.saa.2026.128104_bb0255","doi-asserted-by":"crossref","DOI":"10.1016\/j.molstruc.2024.140592","article-title":"Study on the interaction mechanism of trypsin\/pepsin-doxycycline hyclate-quercetin ternary system","volume":"1322","author":"Li","year":"2025","journal-title":"J. Mol. Struct."},{"key":"10.1016\/j.saa.2026.128104_bb0260","series-title":"Circular dichroism and the conformational analysis of biomolecules","author":"Fasman","year":"2013"},{"key":"10.1016\/j.saa.2026.128104_bb0265","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2022.120194","article-title":"A comprehensive insight into the effects of punicalagin on pepsin: multispectroscopy and simulations methods","volume":"365","author":"Yue","year":"2022","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.saa.2026.128104_bb0270","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2022.120095","article-title":"Comparison of the interactions of fanetizole with pepsin and trypsin: spectroscopic and molecular docking approach","volume":"365","author":"Yue","year":"2022","journal-title":"J. Mol. Liq."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S138614252600675X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S138614252600675X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T21:54:11Z","timestamp":1784238851000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S138614252600675X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":54,"alternative-id":["S138614252600675X"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2026.128104","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Explore the interaction mechanism between artificial synthetic food colorants and digestive proteases via multi-spectral technology and computer simulation","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2026.128104","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128104"}}