{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:32:59Z","timestamp":1793421179208,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007480","name":"University of Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007480","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011698","name":"Regional Government of Castilla-La Mancha","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011698","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Gobierno de Espana Ministerio de Ciencia e Innovacion","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.saa.2026.128487","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:30:11Z","timestamp":1784676611000},"page":"128487","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P2","title":["Fluorescence lifetime mapping of hydrophobic microenvironments via self-assembly of a 7-azaindole probe"],"prefix":"10.1016","volume":"363","author":[{"given":"Mar\u00eda","family":"Rosell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo","family":"Ripoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"\u00c1lvarez-Conde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Garz\u00f3n-Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M.","family":"Garc\u00eda-Frutos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2026.128487_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.physa.2021.126189","article-title":"Hydrophilicity and hydrophobicity: key aspects for biomedical and technological purposes","volume":"580","author":"Corsaro","year":"2021","journal-title":"Phys. A"},{"key":"10.1016\/j.saa.2026.128487_bb0010","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1021\/acs.chemrev.3c00573","article-title":"Design and application of fluorescent probes to detect cellular physical microenvironments","volume":"124","author":"Ma","year":"2024","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2026.128487_bb0015","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1039\/D1MD00170A","article-title":"Polarity-based fluorescence probes: properties and applications","volume":"12","author":"Qin","year":"2021","journal-title":"RSC Med. Chem."},{"key":"10.1016\/j.saa.2026.128487_bb0020","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1021\/acs.accounts.6b00292","article-title":"Fluorescent probes for sensing and imaging within specific cellular organelles","volume":"49","author":"Zhu","year":"2016","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.saa.2026.128487_bb0025","doi-asserted-by":"crossref","DOI":"10.1038\/srep18337","article-title":"BODIPY-based fluorescent probes for sensing protein surface hydrophobicity","volume":"5","author":"Dorh","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.saa.2026.128487_bb0030","doi-asserted-by":"crossref","first-page":"7577","DOI":"10.1039\/D4TB00567H","article-title":"Protein aggregation monitoring in cells under oxidative stress: a novel fluorescent probe based on a 7-azaindole-BODIPY derivative","volume":"12","author":"Herrera-Ochoa","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.saa.2026.128487_bb0035","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1021\/la00070a011","article-title":"Pyrene excimer formations in micelles of nonionic detergents and of water-soluble polymers","volume":"2","author":"Turro","year":"1986","journal-title":"Langmuir"},{"key":"10.1016\/j.saa.2026.128487_bb0040","article-title":"Unlocking new frontiers in bioimaging: the power of FLIM-AIE integration","volume":"7","author":"Blas-G\u00f3mez","year":"2025","journal-title":"Innovation (Cambridge (Mass.))"},{"key":"10.1016\/j.saa.2026.128487_bb0045","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1021\/cr900343z","article-title":"Fluorescence lifetime measurements and biological imaging","volume":"110","author":"Berezin","year":"2010","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2026.128487_bb0050","series-title":"FLIM strategies for intracellular sensing","author":"Ruedas-Rama","year":"2014"},{"key":"10.1016\/j.saa.2026.128487_bb0055","doi-asserted-by":"crossref","DOI":"10.1002\/cben.70006","article-title":"Advances and challenges in fluorescence-based biosensors for biological and chemical detection","volume":"12","author":"Alharthi","year":"2025","journal-title":"ChemBioEng Rev."},{"key":"10.1016\/j.saa.2026.128487_bb0060","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1021\/jacsau.5c00314","article-title":"Advancing multicolor super-resolution volume imaging: illuminating complex cellular dynamics","volume":"5","author":"Rabiee","year":"2025","journal-title":"JACS Au"},{"key":"10.1016\/j.saa.2026.128487_bb0065","doi-asserted-by":"crossref","first-page":"3142","DOI":"10.1039\/c001897j","article-title":"Luminescence and reactivity of 7-azaindole derivatives and complexes","volume":"39","author":"Zhao","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.saa.2026.128487_bb0070","doi-asserted-by":"crossref","first-page":"8663","DOI":"10.1021\/j100175a046","article-title":"Photophysics of a novel optical probe: 7-azaindole","volume":"95","author":"Negrerie","year":"1991","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.saa.2026.128487_bb0075","doi-asserted-by":"crossref","first-page":"1702176","DOI":"10.1002\/adfm.201702176","article-title":"Self-assembling azaindole organogel for organic light-emitting devices (OLEDs)","volume":"27","author":"Mart\u00edn","year":"2017","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.saa.2026.128487_bb0080","doi-asserted-by":"crossref","first-page":"2402859","DOI":"10.1002\/adfm.202402859","article-title":"Lasing in crystals based on 7-azaindole-phenylhydrazone organoboron compounds","volume":"35","author":"\u00c1lvarez-Conde","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.saa.2026.128487_bb0085","series-title":"Gaussian 16, Revision C.01","author":"Frisch","year":"2016"},{"key":"10.1016\/j.saa.2026.128487_bb0090","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1103\/PhysRevLett.77.3865","article-title":"Generalized gradient approximation made simple","volume":"77","author":"Perdew","year":"1996","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.saa.2026.128487_bb0095","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1002\/jcc.10189","article-title":"Energies, structures, and electronic properties of molecules in solution with the C-PCM solvation model","volume":"24","author":"Cossi","year":"2003","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.saa.2026.128487_bb0100","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1021\/cr9904009","article-title":"Quantum mechanical continuum solvation models","volume":"105","author":"Tomasi","year":"2005","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2026.128487_bb0105","doi-asserted-by":"crossref","DOI":"10.1063\/1.1867373","article-title":"Electronic excitation energies of molecules in solution: state specific and linear response methods for nonequilibrium continuum solvation models","volume":"122","author":"Cammi","year":"2005","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.saa.2026.128487_bb0110","doi-asserted-by":"crossref","first-page":"154104","DOI":"10.1063\/1.3382344","article-title":"A consistent and accurate ab initio parametrization of density functional dispersion correction (DFT-D) for the 94 elements H\u2013Pu","volume":"132","author":"Grimme","year":"2010","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.saa.2026.128487_bb0115","doi-asserted-by":"crossref","first-page":"3118","DOI":"10.1021\/ct400337e","article-title":"On the metric of charge transfer molecular excitations: a simple chemical descriptor","volume":"9","author":"Guido","year":"2013","journal-title":"J. Chem. Theory Comput."},{"key":"10.1016\/j.saa.2026.128487_bb0120","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/jcc.22885","article-title":"Multiwfn: a multifunctional wavefunction analyzer","volume":"33","author":"Lu","year":"2012","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.saa.2026.128487_bb0125","doi-asserted-by":"crossref","first-page":"8338","DOI":"10.1039\/c0jm03711g","article-title":"Polymorphic and mechanochromic luminescence modulation in the highly emissive dicyanodistyrylbenzene crystal: secondary bonding interaction in molecular stacking assembly","volume":"21","author":"Yoon","year":"2011","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.saa.2026.128487_bb0130","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0301-0104(00)00336-0","article-title":"A theoretical study of the absorption spectra of indole and its analogs: indene, benzimidazole, and 7-azaindole","volume":"262","author":"Borin","year":"2000","journal-title":"Chem. Phys."},{"key":"10.1016\/j.saa.2026.128487_bb0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2025.125699","article-title":"Unusual high fluorescence of a 7,7\u2032-diazaisoindigo derivative: a photophysical study","volume":"330","author":"Moral","year":"2025","journal-title":"Spectrochim. Acta A"},{"key":"10.1016\/j.saa.2026.128487_bb0140","first-page":"3373","article-title":"Understanding the driving mechanisms of enhanced luminescence emission of oligo(styryl)benzenes and tri(styryl)-s-triazine","volume":"26","author":"Dom\u00ednguez","year":"2020","journal-title":"Chemistry (Weinheim an der Bergstrasse, Germany)"},{"key":"10.1016\/j.saa.2026.128487_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.dyepig.2023.111179","article-title":"Effect of bulky side groups on photophysical properties and electroluminescent performance of oligo(styryl)benzenes","volume":"213","author":"de Lera-Garrido","year":"2023","journal-title":"Dyes Pigments"},{"key":"10.1016\/j.saa.2026.128487_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.dyepig.2021.109427","article-title":"Styrylbenzene organogels and how the cyano groups tune the aggregation-induced emission","volume":"192","author":"Dom\u00ednguez","year":"2021","journal-title":"Dyes Pigments"},{"key":"10.1016\/j.saa.2026.128487_bb0155","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.cplett.2010.05.022","article-title":"Evidence for the phantom state in photoinduced cis\u2013trans isomerization of stilbene","volume":"493","author":"Kovalenko","year":"2010","journal-title":"Chem. Phys. Lett."},{"key":"10.1016\/j.saa.2026.128487_bb0160","doi-asserted-by":"crossref","first-page":"23166","DOI":"10.1021\/acs.jpcc.7b08060","article-title":"Solid state luminescence enhancement in \u03c0-conjugated materials: unraveling the mechanism beyond the framework of AIE\/AIEE","volume":"121","author":"Shi","year":"2017","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.saa.2026.128487_bb0165","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1515\/zna-1988-0609","article-title":"Fluoreszenz-untersuchungen an distyrylbenzolen","volume":"43","author":"Oelkrug","year":"1988","journal-title":"Zeitschrift f\u00fcr Naturforschung A"},{"key":"10.1016\/j.saa.2026.128487_bb0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.jphotochem.2020.112881","article-title":"Effect of the transition rate between two excited states on the spectral dynamics of dual fluorescence: blurring of the isoemissive point","volume":"404","author":"Nazarov","year":"2021","journal-title":"J. Photochem. Photobiol. A"},{"key":"10.1016\/j.saa.2026.128487_bb0175","doi-asserted-by":"crossref","DOI":"10.1002\/cbic.202200744","article-title":"Quality data from messy spectra: how isometric points increase information content in highly overlapping spectra","volume":"24","author":"Kaspar","year":"2023","journal-title":"ChemBioChem"},{"key":"10.1016\/j.saa.2026.128487_bb0180","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jmb.2005.07.075","article-title":"Structural basis of the drug-binding specificity of human serum albumin","volume":"353","author":"Ghuman","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.saa.2026.128487_bb0185","doi-asserted-by":"crossref","first-page":"32047","DOI":"10.1021\/acsami.8b13749","article-title":"Temperature-induced denaturation of BSA protein molecules for improved surface passivation coatings","volume":"10","author":"Park","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.saa.2026.128487_bb0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodhyd.2019.105450","article-title":"Mg2+ inhibits heat-induced aggregation of BSA: the mechanism and its binding site","volume":"101","author":"Liu","year":"2020","journal-title":"Food Hydrocoll."},{"key":"10.1016\/j.saa.2026.128487_bb0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2020.118975","article-title":"Molecular mechanisms causing albumin aggregation: the main role of the porphyrins of the blood group","volume":"246","author":"Lebedeva","year":"2021","journal-title":"Spectrochim. Acta A"},{"key":"10.1016\/j.saa.2026.128487_bb0200","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1021\/acs.molpharmaceut.8b00115","article-title":"Protein aggregation and performance optimization based on microconformational changes of aromatic hydrophobic regions","volume":"15","author":"Wen","year":"2018","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.saa.2026.128487_bb0205","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/nrm.2017.16","article-title":"The mystery of membrane organization: composition, regulation and roles of lipid rafts","volume":"18","author":"Sezgin","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.saa.2026.128487_bb0210","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S0968-0004(01)01938-7","article-title":"Macromolecular crowding: obvious but underappreciated","volume":"26","author":"Ellis","year":"2001","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.saa.2026.128487_bb0215","doi-asserted-by":"crossref","first-page":"6672","DOI":"10.1021\/ja800570d","article-title":"Molecular rotor measures viscosity of live cells via fluorescence lifetime imaging","volume":"130","author":"Kuimova","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.saa.2026.128487_bb0220","doi-asserted-by":"crossref","first-page":"13327","DOI":"10.1073\/pnas.1703781114","article-title":"Water is an active matrix of life for cell and molecular biology","volume":"114","author":"Ball","year":"2017","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.saa.2026.128487_bb0225","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1021\/acs.accounts.6b00517","article-title":"Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications","volume":"50","author":"Klymchenko","year":"2017","journal-title":"Acc. Chem. Res."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526010589?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526010589?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:29:42Z","timestamp":1793420982000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142526010589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":45,"alternative-id":["S1386142526010589"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2026.128487","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Fluorescence lifetime mapping of hydrophobic microenvironments via self-assembly of a 7-azaindole probe","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2026.128487","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"128487"}}