{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:02:56Z","timestamp":1790625776358,"version":"4.1.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.saa.2026.128745","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:50:07Z","timestamp":1788796207000},"page":"128745","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Single-cell Raman spectroscopy combined with deep learning for antimicrobial resistance detection in Mycobacterium abscessus"],"prefix":"10.1016","volume":"365","author":[{"given":"Junhao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoujie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengchan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2026.128745_bb0005","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ccm.2014.10.003","article-title":"Environmental sources of nontuberculous mycobacteria","volume":"36","author":"Falkinham","year":"2015","journal-title":"Clin. Chest Med."},{"key":"10.1016\/j.saa.2026.128745_bb0010","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1164\/rccm.200604-571ST","article-title":"An official ATS\/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases","volume":"175","author":"Griffith","year":"2007","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.saa.2026.128745_bb0015","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1093\/jac\/dkr578","article-title":"Mycobacterium abscessus: a new antibiotic nightmare","volume":"67","author":"Nessar","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.saa.2026.128745_bb0020","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in vitro evaluating antimicrobial activity: a review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J Pharm Anal"},{"key":"10.1016\/j.saa.2026.128745_bb0025","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1007\/s10096-015-2424-8","article-title":"Evaluation of the PREVI\u00ae Isola automated seeder system compared to reference manual inoculation for antibiotic susceptibility testing by the disk diffusion method","volume":"34","author":"Le Page","year":"2015","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.saa.2026.128745_bb0030","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.saa.2026.128745_bb0035","article-title":"Current and emerging methods of antibiotic susceptibility testing","volume":"9","author":"Khan","year":"2019","journal-title":"Diagnostics (Basel)"},{"key":"10.1016\/j.saa.2026.128745_bb0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.sjbs.2023.103582","article-title":"Conventional methods and future trends in antimicrobial susceptibility testing","volume":"30","author":"Salam","year":"2023","journal-title":"Saudi J. Biol. Sci."},{"key":"10.1016\/j.saa.2026.128745_bb0045","doi-asserted-by":"crossref","first-page":"5098","DOI":"10.1021\/acs.analchem.0c04709","article-title":"Development of a fast Raman-assisted antibiotic susceptibility test (FRAST) for the antibiotic resistance analysis of clinical urine and blood samples","volume":"93","author":"Yi","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128745_bb0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.338622","article-title":"Rapid antimicrobial susceptibility testing by stimulated Raman scattering metabolic imaging and morphological deformation of bacteria","volume":"1168","author":"Zhang","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.saa.2026.128745_bb0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2024.126354","article-title":"Micro-flow cell washing technique combined with single-cell Raman spectroscopy for rapid and automatic antimicrobial susceptibility test of pathogen in urine","volume":"277","author":"Zhao","year":"2024","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128745_bb0060","doi-asserted-by":"crossref","first-page":"4927","DOI":"10.1038\/s41467-019-12898-9","article-title":"Rapid identification of pathogenic bacteria using Raman spectroscopy and deep learning","volume":"10","author":"Ho","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.saa.2026.128745_bb0065","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.copbio.2011.11.019","article-title":"Single cell Raman spectroscopy for cell sorting and imaging","volume":"23","author":"Li","year":"2012","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.saa.2026.128745_bb0070","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.copbio.2016.04.018","article-title":"Single cell stable isotope probing in microbiology using Raman microspectroscopy","volume":"41","author":"Wang","year":"2016","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.saa.2026.128745_bb0075","doi-asserted-by":"crossref","first-page":"34359","DOI":"10.1038\/srep34359","article-title":"Label-free, rapid and quantitative phenotyping of stress response in E. Coli via ramanome","volume":"6","author":"Teng","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.saa.2026.128745_bb0080","doi-asserted-by":"crossref","first-page":"E194","DOI":"10.1073\/pnas.1420406112","article-title":"Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells","volume":"112","author":"Berry","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.saa.2026.128745_bb0085","article-title":"Single-cell Raman spectroscopy identifies Escherichia coli persisters and reveals their enhanced metabolic activities","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.saa.2026.128745_bb0090","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1111\/1751-7915.13960","article-title":"Identification of antibiotic resistance and virulence-encoding factors in Klebsiella pneumoniae by Raman spectroscopy and deep learning","volume":"15","author":"Lu","year":"2022","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.saa.2026.128745_bb0095","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.bsheal.2025.03.004","article-title":"Detecting and classifying metabolic activity of Staphylococcus aureus by D2O-probed single-cell Raman spectroscopy and machine learning","volume":"7","author":"Liu","year":"2025","journal-title":"Biosaf. Health"},{"key":"10.1016\/j.saa.2026.128745_bb0100","doi-asserted-by":"crossref","first-page":"4108","DOI":"10.1021\/acs.analchem.6b05051","article-title":"Metabolic-activity-based assessment of antimicrobial effects by D(2)O-Labeled single-cell Raman microspectroscopy","volume":"89","author":"Tao","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128745_bb0105","doi-asserted-by":"crossref","first-page":"15171","DOI":"10.1021\/acs.analchem.9b03960","article-title":"Revealing the metabolic activity of Persisters in mycobacteria by single-cell D(2)O Raman imaging spectroscopy","volume":"91","author":"Ueno","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128745_bb0110","article-title":"Assessing efficacy of clinical disinfectants for pathogenic Fungi by single-cell Raman microspectroscopy","volume":"12","author":"Li","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.saa.2026.128745_bb0115","article-title":"An isotope-Labeled single-cell Raman spectroscopy approach for tracking the physiological evolution trajectory of Bacteria toward antibiotic resistance","volume":"62","author":"Yang","year":"2023","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.saa.2026.128745_bb0120","series-title":"Clinical and Laboratory Standards Institute Copyright \u00a92011 Clinical and Laboratory Standards Institute","article-title":"Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes","author":"Woods","year":"2011"},{"key":"10.1016\/j.saa.2026.128745_bb0125","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1002\/jcla.21933","article-title":"Resazurin microtiter assay for clarithromycin susceptibility testing of clinical isolates of mycobacterium abscessus group","volume":"30","author":"Garcia de Carvalho","year":"2016","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.saa.2026.128745_bb0130","article-title":"Robust spontaneous Raman flow cytometry for single-cell metabolic phenome profiling via pDEP-DLD-RFC","volume":"10","author":"Wang","year":"2023","journal-title":"Adv Sci (Weinh)"},{"key":"10.1016\/j.saa.2026.128745_bb0135","doi-asserted-by":"crossref","first-page":"eabb3521","DOI":"10.1126\/sciadv.abb3521","article-title":"Positive dielectrophoresis-based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo","volume":"6","author":"Wang","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.saa.2026.128745_bb0140","doi-asserted-by":"crossref","first-page":"3631","DOI":"10.1021\/ac034173t","article-title":"A perfect smoother","volume":"75","author":"Eilers","year":"2003","journal-title":"Anal. Chem."},{"key":"10.1016\/j.saa.2026.128745_bb0145","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1109\/72.788640","article-title":"An overview of statistical learning theory","volume":"10","author":"Vapnik","year":"1999","journal-title":"IEEE Trans. Neural Netw."},{"key":"10.1016\/j.saa.2026.128745_bb0150","article-title":"Deep learning in analytical chemistry","volume":"145","author":"Bruno","year":"2021","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.saa.2026.128745_bb0155","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/nature14539","article-title":"Deep learning","volume":"521","author":"LeCun","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.saa.2026.128745_bb0160","first-page":"818","article-title":"Visualizing and understanding convolutional networks","volume":"abs\/1311.2901","author":"Zeiler","year":"2013","journal-title":"CoRR"},{"key":"10.1016\/j.saa.2026.128745_bb0165","doi-asserted-by":"crossref","first-page":"5962","DOI":"10.1109\/TPAMI.2021.3087709","article-title":"ArcFace: additive angular margin loss for deep face recognition","volume":"44","author":"Deng","year":"2022","journal-title":"IEEE Trans. Pattern Anal. Mach. Intell."},{"key":"10.1016\/j.saa.2026.128745_bb0170","article-title":"Adam: a method for stochastic optimization","volume":"abs\/1412.6980","author":"Kingma","year":"2014","journal-title":"CoRR"},{"key":"10.1016\/j.saa.2026.128745_bb0175","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.patrec.2005.10.010","article-title":"An introduction to ROC analysis","volume":"27","author":"Fawcett","year":"2006","journal-title":"Pattern Recogn. Lett."},{"key":"10.1016\/j.saa.2026.128745_bb0215","article-title":"Liquid crystal-amplified optofluidic biosensor for ultra-highly sensitive and stable protein assay","volume":"2","author":"Ziyihui","year":"2021","journal-title":"PhotoniX"},{"key":"10.1016\/j.saa.2026.128745_bb0220","first-page":"115970","article-title":"Hollow-microsphere-integrated optofluidic immunochip for myocardial infarction biomarker microanalysis","volume":"248","author":"Panpan","year":"2023","journal-title":"Biosens. Bioelectron."}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526013168?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526013168?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:33:38Z","timestamp":1790624018000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142526013168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":37,"alternative-id":["S1386142526013168"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2026.128745","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-cell Raman spectroscopy combined with deep learning for antimicrobial resistance detection in Mycobacterium abscessus","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2026.128745","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128745"}}