{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:51:44Z","timestamp":1791661904983,"version":"4.3.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.saa.2026.128910","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:15:46Z","timestamp":1790810146000},"page":"128910","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P1","title":["Modular secondary-antibody-based SERS immunoprobes for detecting membrane-associated proteins in adherent cells"],"prefix":"10.1016","volume":"367","author":[{"given":"Yuxuan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianghui","family":"Mian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lufeng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiutong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.saa.2026.128910_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116819","article-title":"In situ surface-enhanced raman spectroscopy for membrane protein analysis and sensing","volume":"267","author":"Xu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128910_bb0010","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1021\/acs.chemrev.4c00554","article-title":"Engineered proteins and chemical tools to probe the cell surface proteome","volume":"125","author":"Leung","year":"2025","journal-title":"Chem. Rev."},{"key":"10.1016\/j.saa.2026.128910_bb0015","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.jcis.2024.04.170","article-title":"Single-cell SERS imaging of dual cell membrane receptors expression influenced by extracellular matrix stiffness","volume":"668","author":"Liu","year":"2024","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.saa.2026.128910_bb0020","article-title":"Multiplexed integrin detection and cancer cell classification using multicolor gap-enhanced gold nanorods and machine learning algorithm","volume":"15","author":"Shah","year":"2025","journal-title":"Nanomaterials (Basel, Switzerland)"},{"key":"10.1016\/j.saa.2026.128910_bb0025","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1038\/s41592-024-02268-9","article-title":"Molecular pixelation: spatial proteomics of single cells by sequencing","volume":"21","author":"Karlsson","year":"2024","journal-title":"Nat. Methods"},{"key":"10.1016\/j.saa.2026.128910_bb0030","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25042080","article-title":"Immunoassays: analytical and clinical performance, challenges, and perspectives of SERS detection in comparison with fluorescent spectroscopic detection","volume":"25","author":"Terzapulo","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.saa.2026.128910_bb0035","doi-asserted-by":"crossref","first-page":"8957","DOI":"10.1039\/D4CS00090K","article-title":"Biomedical SERS - the current state and future trends","volume":"53","author":"Cialla-May","year":"2024","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.saa.2026.128910_bb0040","article-title":"Design and synthesis of SERS materials for In vivo molecular imaging and biosensing","volume":"10","author":"Li","year":"2023","journal-title":"Adv. Sci. (Weinh. Baden-Wurtt. Ger.)"},{"key":"10.1016\/j.saa.2026.128910_bb0045","doi-asserted-by":"crossref","first-page":"6675","DOI":"10.1039\/D3CS01060K","article-title":"ISERS: from nanotag design to protein assays and ex vivo imaging","volume":"53","author":"Choi","year":"2024","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.saa.2026.128910_bb0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2023.115366","article-title":"Highly selective and quantitative in situ monitoring of cell surface proteins by SERS immunoassay system","volume":"234","author":"Park","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128910_bb0055","doi-asserted-by":"crossref","first-page":"32321","DOI":"10.1021\/acsami.0c07269","article-title":"6-color\/1-target immuno-SERS microscopy on the same single cancer cell","volume":"12","author":"Stepula","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.saa.2026.128910_bb0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2022.123900","article-title":"In-situ and amplification-free imaging of hERG ion channels at single-cell level using a unique core-molecule-shell-secondary antibody SERS nanoprobe","volume":"253","author":"Zhang","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128910_bb0065","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jcis.2024.02.113","article-title":"AMP coated SERS NanoTags with hydrophobic locking: maximizing brightness, stability, and cellular targetability","volume":"663","author":"Lane","year":"2024","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.saa.2026.128910_bb0070","doi-asserted-by":"crossref","DOI":"10.1186\/s40580-024-00443-4","article-title":"Advancing SERS as a quantitative technique: challenges, considerations, and correlative approaches to aid validation","volume":"11","author":"Sloan-Dennison","year":"2024","journal-title":"Nano Convergence"},{"key":"10.1016\/j.saa.2026.128910_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.124818","article-title":"SERS immuno- and apta-assays in biosensing\/bio-detection: performance comparison, clinical applications, challenges","volume":"265","author":"Ilyas","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128910_bb0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.canlet.2025.217713","article-title":"CD44 identified as a diagnostic biomarker for highly malignant CA19\u20139 negative pancreatic cancer","volume":"622","author":"Tang","year":"2025","journal-title":"Cancer Lett."},{"key":"10.1016\/j.saa.2026.128910_bb0085","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.714841","article-title":"Kv1.3 channel up-regulation in peripheral blood T lymphocytes of patients with multiple sclerosis","volume":"12","author":"Markakis","year":"2021","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.saa.2026.128910_bb0090","article-title":"A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis","volume":"120","author":"Wang","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.saa.2026.128910_bb0095","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/anie.201810917","article-title":"Rapid, quantitative, and ultrasensitive point-of-care testing: a portable SERS reader for lateral flow assays in clinical chemistry","volume":"58","author":"Tran","year":"2019","journal-title":"Angew. Chem."},{"key":"10.1016\/j.saa.2026.128910_bb0100","doi-asserted-by":"crossref","first-page":"11012","DOI":"10.1039\/D4TB01367K","article-title":"Combined and rapid detection of anti-brucella IgG\/IgM in clinical samples based on surface-enhanced raman scattering-lateral flow immunochromatography","volume":"12","author":"Zhang","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.saa.2026.128910_bb0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2024.108472","article-title":"Harnessing raman spectroscopy for cell therapy bioprocessing","volume":"77","author":"Costa","year":"2024","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.saa.2026.128910_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.saa.2025.126160","article-title":"Synthesis and characterization of Au@Ag nanoparticles for multiwavelength SERS biosensing","volume":"338","author":"Sirgedaite","year":"2025","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.saa.2026.128910_bb0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116973","article-title":"Digital SERS immunoassay of interleukin-6 based on Au@Ag-Au nanotags","volume":"270","author":"Li","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128910_bb0120","doi-asserted-by":"crossref","DOI":"10.1002\/smsc.202400259","article-title":"Pushing the limits of lateral flow immunoassay by digital SERS for the ultralow detection of SARS-CoV-2 virus","volume":"4","author":"Gonz\u00e1lez-Cabaleiro","year":"2024","journal-title":"Small Science"},{"key":"10.1016\/j.saa.2026.128910_bb0125","first-page":"248","article-title":"SERS sensing for cancer biomarker: approaches and directions","volume":"34","author":"V\u00e1zquez-Iglesias","year":"2024","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.saa.2026.128910_bb0130","doi-asserted-by":"crossref","DOI":"10.1002\/wnan.70015","article-title":"Surface-enhanced raman scattering nanotags: design strategies, biomedical applications, and integration of machine learning","volume":"17","author":"Vasquez","year":"2025","journal-title":"WIREs Nanomed. Nanobiotechnol."},{"key":"10.1016\/j.saa.2026.128910_bb0135","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-024-52205-9","article-title":"Hybrid lipid-AuNP clusters as highly efficient SERS substrates for biomedical applications","volume":"15","author":"Cardellini","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.saa.2026.128910_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2025.146364","article-title":"Reusable Ag-nanocube-embedded SERS platform for quantitative detection of spoilage yeasts in kimchi and finite-difference time-domain (FDTD)-guided hotspot design strategy","volume":"495","author":"Lee","year":"2025","journal-title":"Food Chem."},{"key":"10.1016\/j.saa.2026.128910_bb0145","doi-asserted-by":"crossref","first-page":"5394","DOI":"10.1039\/D3CS01055D","article-title":"SERS-based microdevices for use as in vitro diagnostic biosensors","volume":"53","author":"Lee","year":"2024","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.saa.2026.128910_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2022.123888","article-title":"Core-shell SERS nanotags-based western blot","volume":"253","author":"Liu","year":"2023","journal-title":"Talanta"},{"key":"10.1016\/j.saa.2026.128910_bb0155","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1021\/acssensors.3c00100","article-title":"Ultrasensitive multiplex imaging of cell surface proteins via core-shell surface-enhanced raman scattering nanoprobes","volume":"8","author":"Wang","year":"2023","journal-title":"ACS Sens."},{"key":"10.1016\/j.saa.2026.128910_bb0160","doi-asserted-by":"crossref","first-page":"11257","DOI":"10.1021\/acsnano.4c00543","article-title":"A scaffold-assisted 3D cancer cell model for surface-enhanced raman scattering-based real-time sensing and imaging","volume":"18","author":"Garc\u00eda-Astrain","year":"2024","journal-title":"ACS Nano"},{"key":"10.1016\/j.saa.2026.128910_bb0165","doi-asserted-by":"crossref","first-page":"37747","DOI":"10.1021\/acsami.5c08079","article-title":"A permeabilization workflow to enable specific multiplexed profiling using SERS nanoparticles","volume":"17","author":"Bagheri","year":"2025","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.saa.2026.128910_bb0170","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1186\/s12951-024-02600-7","article-title":"Combined SERS-raman screening of HER2-overexpressing or silenced breast cancer cell lines","volume":"22","author":"Spaziani","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.saa.2026.128910_bb0175","doi-asserted-by":"crossref","first-page":"12869","DOI":"10.1039\/D3SC04650H","article-title":"Surface-enhanced raman scattering sensing for detection and mapping of key cellular biomarkers","volume":"14","author":"Yang","year":"2023","journal-title":"Chem. Sci."},{"key":"10.1016\/j.saa.2026.128910_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116964","article-title":"A fluorescence and SERS dual-mode biosensor for quantification and imaging of Mucin1 in living cells","volume":"270","author":"Wang","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.saa.2026.128910_bb0185","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1002\/med.21800","article-title":"Discovery of KV 1.3 ion channel inhibitors: medicinal chemistry approaches and challenges","volume":"41","author":"Gubi\u010d","year":"2021","journal-title":"Med. Res. Rev."},{"key":"10.1016\/j.saa.2026.128910_bb0190","doi-asserted-by":"crossref","first-page":"15847","DOI":"10.1021\/acsami.3c18959","article-title":"A systematic approach toward enabling maximal targeting efficiency of cell surface proteins with actively targeted SERS nanoparticles","volume":"16","author":"Bagheri","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"}],"container-title":["Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526014812?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386142526014812?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:37:36Z","timestamp":1791661056000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386142526014812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":38,"alternative-id":["S1386142526014812"],"URL":"https:\/\/doi.org\/10.1016\/j.saa.2026.128910","relation":{},"ISSN":["1386-1425"],"issn-type":[{"value":"1386-1425","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Modular secondary-antibody-based SERS immunoprobes for detecting membrane-associated proteins in adherent cells","name":"articletitle","label":"Article Title"},{"value":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.saa.2026.128910","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"128910"}}